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Citations for Millipore Sigma :
5701 - 5750 of 10000+ citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... at room temperature with a thick layer of self-indicating silica gel (a granular material with c. 3 to 5mm irregular beads; e.g. Type III Sigma-Aldrich, S7625 ...
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bioRxiv - Physiology 2019Quote: Mouse islets were isolated as previously described61 using Type XI collagenase (1000 units/mL; #C7657, Sigma-Aldrich, St. Louis, MO). Following digestion and filtration ...
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bioRxiv - Physiology 2021Quote: ... TREH2B-GFP mice were collected and incubated in high-glucose DMEM (HyClone Laboratories) with 0.3% collagenase type I (Sigma-Aldrich) at 37°C for 40 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... the eye cup was rinsed with sterile PBS and the interior was digested using 0.5 mg/ml each of collagenase Type I-A (from Clostridium histolyticum, Sigma-Aldrich) and Type IV (from Clostridium ...
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bioRxiv - Biochemistry 2021Quote: ... wild-type (WT) or D274N were individually expressed from a pET30-TEV vector in BL21 (DE3) pLysS Escherichia coli (Novagen), with protein expression being induced with 0.4 mM IPTG for 18 h at 18 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... the GBM tissue was cut into small pieces and incubated with dissociation medium (200 μL Collagenase type I (10 mg / mL, Sigma), 200 μL Dispase II (20 mg / mL ...
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bioRxiv - Developmental Biology 2021Quote: ... mRNA was first isolated from 40 dpf wild-type AB strain ovaries using TRI reagent (Cat. No. T9424; Sigma-Aldrich) and then cDNAs were synthesized with RETROScript Reverse Transcription Kit (Cat ...
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bioRxiv - Pathology 2021Quote: ... Splenocytes were isolated from wild-type mouse spleens and cultured overnight with 100ng/ml LPS and 5ug/ml PHA (Sigma). The cells were then labeled with cell trace violet (CTV ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was discarded and liver tissue pieces were resuspended in 10 mL of Dissociation buffer [Liver Wash Buffer with 0.15mg/mL collagenase type XI (Sigma, C9407), 0.3U/mL Dispase (Corning ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Seeds were surface-sterilized and sown in Petri dishes on half-strength Murashige and Skoog (MS/2) solid medium containing 0.8 % (w/v) agar-agar type E (Sigma Aldrich) and 1% sucrose ...
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bioRxiv - Microbiology 2020Quote: ... we coated the well with 0.5 mg/ml Concanavalin A from Canavalia ensiformis (Jack bean) Type IV-S (lyophilized powder, aseptically processed, Sigma-Aldrich). Each well was loaded with 300 µL of Concanavalin A solution (dissolved in water ...
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bioRxiv - Immunology 2021Quote: ... the ovaries were cut using scissors and incubated for 30 minutes with ~7,800 IU/mL (Lot dependent) of collagenase type IV (Sigma-Aldrich) at 37°C ...
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bioRxiv - Immunology 2020Quote: ... Dissociated DRGs were washed in supplemented DMEM and were triturated with glass pipettes of decreasing sizes into 2 mL of fully supplemented DMEM and 62.5 U/mL Deoxyribonuclease I type IV (Sigma-Aldrich). The DRG cells were then passed over a 3% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured as a monolayer in tissue culture flasks coated with 0.1 mg/mL Bovine type I collagen solution (Sigma-Aldrich). At passage two ...
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The intestinal circadian clock drives microbial rhythmicity to maintain gastrointestinal homeostasisbioRxiv - Physiology 2022Quote: ... jejunum tissue was then digested at 37°C for approximately 15 min in Thermoshake (200rpm) with 0.6 mg/ml type VIII collagenase (Sigma-Aldrich). Colonic tissue was simultaneously digested under the same condition for approximately 25 min but with the mixture of 0.85 mg/ml type V collagenase (Sigma-Aldrich) ...
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The intestinal circadian clock drives microbial rhythmicity to maintain gastrointestinal homeostasisbioRxiv - Physiology 2022Quote: ... Colonic tissue was simultaneously digested under the same condition for approximately 25 min but with the mixture of 0.85 mg/ml type V collagenase (Sigma-Aldrich), 1.25 mg/ml collagenase D (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2022Quote: ... and the top channel was filled with a mixture of rat tail type I collagen (Sigma Aldrich, St. Louis, MO) at about 100 μg/mL ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from the wild-type and bsu-q seedlings treated with flg22 or mock solution using the Spectrum Plant Total RNA kit (Sigma). cDNA libraries were constructed using a TruSeq RNA sample preparation kit (Illumina ...
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bioRxiv - Bioengineering 2022Quote: ... according to methods previously published.[28,29] Microcapsules were constituted of 1.0% w/v alginic acid (PH163S2, JRS Pharma GmbH, Germany) and 1.0% w/v gelatin type A (Sigma-Aldrich, Germany). These polymers were dissolved in a solution containing 0.1M HEPES (pH 7.4 ...
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bioRxiv - Developmental Biology 2022Quote: ... were routinely cultured at 37°C and 5% CO2 on dishes coated with 0.1% gelatin type B (Sigma Cat3G9391-100G) in GMEM (Sigma ...
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bioRxiv - Immunology 2022Quote: Dorsal and ventral side ears of either Mas-TRECK mice or wild type (C57BL/6) mice (WT) were injected intradermally (i.d.) with 10 ng of diphtheria toxin (DT) (Sigma-Aldrich) in 20 µl of PBS for one time ...
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bioRxiv - Neuroscience 2022Quote: ... The nerve stub was predominantly composed of sympathetic preganglionic fibers and was dissociated by 0.5% type IV collagenase (C5138, Sigma-Aldrich). During experiments ...
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bioRxiv - Molecular Biology 2023Quote: ... a second perfusion (≈ 50mL) was performed with Dissociation buffer (25mM Hepes, pH7.4, 1mM CaCl2 in 1xHBSS) supplemented with collagenase (type IV, Sigma #C5138)(100U/mL ...
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bioRxiv - Neuroscience 2022Quote: ... 9-week-old male wild-type C57BL/6J mice were fed a diet containing 0.3% (w/w) cuprizone (bis[cyclohexanone]oxaldihydrazone, Sigma-Aldrich) mixed in powdered standard chow ad libitum for 5 weeks ...
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bioRxiv - Physiology 2023Quote: One kidney from each mouse was mechanically dissociated using scissors and digested with 0.156mg/ml Collagenase type XI (Sigma-Aldrich), 0.030mg/ml Hyaluronidase type IV-S (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2023Quote: ... 0.5 mM luminol probe 8-amino-5-chloro-7-phenyl-pyrido[3,4-d]pyridazine-1,4(2H,3H)dione (L-012) (Wako Chemicals, Richmond, Virginia, USA) and 20 μg/mL horseradish peroxidase Type VI-A (Sigma, Saint Louis ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were gently minced and digested at 37[°C for 25[minutes with an enzyme cocktail (5 mg collagenase type I with 2.5 mg trypsin, Sigma-Aldrich) in 5 ml DMEM ...
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bioRxiv - Plant Biology 2024Quote: ... 2.5 cm sections were dissected from excised leaves (cut 3 cm from the leaf tip) and placed on 12 cm2 0.5% agar (type M; Sigma-Aldrich) Petri plates ...
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bioRxiv - Biophysics 2024Quote: ... The remaining iron nitrate salt was allowed to cool then quantified using a colorimetric iron assay kit with 3 technical replicates for each cell type (MAK025, Sigma). The total iron from 3 technical replicates was averaged then divided by the number of cells to obtain calculated pg Fe/cell.
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bioRxiv - Cancer Biology 2023Quote: ... Tumors were dissociated (5 mice per group) mechanically using scissors and enzymatically digested in RPMI containing 0.5LJmg/mL Collagenase type I-S (Sigma-Aldrich), 0.4LJmg/mL Dispase II protease (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FBS and then seeded in 6-well plates (5×105 cells/well) that had been pre-coated the day before with type I collagen (Sigma) dissolved in 0.02 N acetic acid ...
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bioRxiv - Molecular Biology 2024Quote: Immunofluorescence analysis was performed on C2C12 cells grown and differentiated on glass coverslips coated with collagen Type I (Sigma-Aldrich), fixed for 10 min in 10% formalin ...
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bioRxiv - Molecular Biology 2024Quote: ... pneumoniae serotype 3 (ST3) with or without addition of either Hyaluronidase from bovine testes Type I-S (H3506 Sigma-Aldrich, St ...
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Synthetase and Hydrolase Specificity Collectively Excludes 2’-Deoxyguanosine from Bacterial AlarmonebioRxiv - Microbiology 2024Quote: ... and ADP production was quantitatively coupled to the stoichiometric consumption of NADH via the activity of excess pyruvate kinase (PK, type-III from rabbit muscle, Sigma) and lactate dehydrogenase (LDH ...
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bioRxiv - Cell Biology 2024Quote: ... were resuspended at cell densities of 8×106 cells/mL for each cell type into a hydrogel comprised of 6.5mg/mL fibrin (Sigma #341573) and supplemented with 0.2mg/mL fibronectin (Sigma #F1141 ...
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bioRxiv - Pathology 2023Quote: ... and antibiotics on culture plates pre-covered with 0.5% gelatin and 100mg/ml collagen type I (Sigma-Aldrich, C2124-50ML). EC purity was >80% after 1 week of culture ...
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bioRxiv - Immunology 2024Quote: ... Tissue was minced and incubated for 15 min at 37°C with collagenase type IA (Sigma Aldrich; St. Louis, MO) in PBS with 0.5% BSA and 2mM EDTA Following red blood cell lysis ...
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bioRxiv - Immunology 2024Quote: Liver was harvested and cut into pre-warmed 25 mM Hepes 10% FBS RPMI supplemented with 0.4 mg/ml collagenase type VIII (Sigma, C2139) under shaking at 180 rpm for 45 min at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... was employed which was prepared freshly before each measurement: glucose oxidase from Aspergillus niger type VII (0.009 U/µL; Sigma-Aldrich), catalase from bovine liver (594 U/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... and human epithelial type 2 (HEp-2, CCL-23, ATCC) cells were cultured in Dulbecco’s modified eagle medium (DMEM, Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Physiology 2023Quote: ... Jejunum tissue was then digested at 37°C for approximately 15 min in Thermoshake (Gerhardt) with 0.6 mg/ml type VIII collagenase (Sigma-Aldrich). Colonic tissue was digested at 37°C for approximately 25 min with 0.85 mg/ml type V collagenase (Sigma-Aldrich) ...
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bioRxiv - Physiology 2023Quote: ... Colonic tissue was digested at 37°C for approximately 25 min with 0.85 mg/ml type V collagenase (Sigma-Aldrich), 1.25 mg/ml collagenase D (Sigma- Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... the micro-vessels were passed through a 40 μm strainer and backflushed with 2 ml PBS before incubation with the digestion cocktail (1mg/ml of collagenase type IV (Millipore), 10 μg/ml DNAse (Roche ...
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bioRxiv - Immunology 2022Quote: ... a catheter was carefully inserted and the infrarenal part was perfused with sterile isotonic saline containing type I Porcine Pancreatic Elastase (2.5-3 U ml, Sigma-Aldrich) for 5 min at 120 mm Hg ...
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bioRxiv - Microbiology 2022Quote: ... the wild-type H99 and isw1Δ mutant strains were either untreated or were treated with 10 μg/ml FLC (Sigma) in 50 ml YPD media at 30°C until cell densities reached the exponential phase (approximately 6 to 7 hours) ...
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bioRxiv - Developmental Biology 2023Quote: ... The testes were then gently loosened with tweezers and transferred to PBS containing 0.5 mg/ml Collagenase type I (Sigma Aldrich). Tissues were incubated at 37□C for 5-10 min until the seminiferous tubules were dispersed ...
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bioRxiv - Genomics 2023Quote: ... or cell type (roughly 5X106 iPSC-NPCs or 1X106 iPSC-neurons on a 6-well plate, enzymatically detached with Accutase (Millipore) and pelleted with centrifugation for 5 mins at 1,000 G) ...
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bioRxiv - Microbiology 2023Quote: ... and the growth medium was adjusted to a final concentration of 0.5 μg/ml trypsin (porcine Type IX pancreatic trypsin, Sigma Aldrich). Once complete cytopathic effects (CPE ...
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bioRxiv - Cancer Biology 2023Quote: ... age-matched mutant mice together with wild-type negative control C57BL/6J mice were injected intraperitoneally with tamoxifen (Sigma, T5648) in corn oil at a concentration of 2 mg per 20g body weight at age 8 – 12 weeks ...
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bioRxiv - Cell Biology 2022Quote: ... A syringe pump was used to infuse the aorta 100mmHg with saline or saline solution containing 0.04 U / ml of type I porcine pancreatic elastin (Sigma-Aldrich). The perfusion catheter is then removed and the aortomy was repaired without narrowing of the aorta ...