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Citations for Millipore Sigma :
5601 - 5650 of 10000+ citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2022Quote: ... The sections were blocked using 1% BSA for 1 hr and incubated with rabbit anti-hamster ACE2 antibody (Product no. HPA000288, Millipore Sigma, Burlington, MA, USA) overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... the samples were washed 3x for 5min with PBS and incubated with secondary antibodies (1:1000) and Hoechst (Sigma, 1mg/ml stock, 1:1000) diluted in staining solution for 2hrs at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Nitrocellulose membranes were blocked for 1 h with 5 % dried milk resuspended in TBS-Tween 1 % and incubated over-night with primary antibodies: anti-alpha-tubulin (Sigma, T6074, 1:1000 dilution) or anti-UKHC (Santa Cruz Biotechnology ...
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bioRxiv - Genomics 2021Quote: ... 5% normal donkey serum in PBS for 1 hour and incubated at 4°C overnight with primary antibody (EMD Millipore, cat. Ab3786, 1:300), then at room temperature for 1 hour with Alexa Fluor 594-conjugated secondary antibody (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... the calcium free medium was replaced with the cell culture medium with or without E-cadherin blocking monoclonal antibody DECMA-1 (Millipore, 10 µg mL−1), and cells migration was carried out for 4 hours at 37°C in a 5% CO2 humidified incubator.
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bioRxiv - Cell Biology 2022Quote: ... Sections were then co-labelled overnight at 4°C with primary antibodies directed against Paired Box 7 (Pax7; DSHB; 1:50) and Laminin α1 (Sigma-Aldrich #L9393; 1:100) in 2% BSA ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated in primary antibodies (diluted in the blocking solution, see Table 1) against myc (rabbit c-Myc, 1:1000, Sigma-Aldrich, C3956, RRID: AB_439680) and FLAG (mouse-FLAG ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunofluorescence staining was performed as previously described [16], using primary antibodies for DR5 (1:100; Cell Signalling Technologies, cat. 8074) and α-tubulin (1:500; Sigma-Aldrich, cat. T9026). Secondary antibodies used were goat anti-rabbit AF488 (1:500 ...
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bioRxiv - Pathology 2024Quote: ... The membranes were blocked with 3% bovine serum albumin in 0.5% Tween-20/Tris-buffered saline (TTBS) for 1 h and incubated with an anti-2,4-dinitrophenylhydrazone (DNP) adducts polyclonal antibody (1:100, EMD Millipore, Billerica, MA, USA, #MAB2223) or HNE polyclonal antibody (1:2000 ...
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bioRxiv - Neuroscience 2024Quote: ... Filters were washed three times with TTBS buffer and then incubated for 1 hour with secondary peroxidase-coupled anti-rabbit antibody (1:7000, Millipore, Cat #401393-2 ML). Immunostaining was revealed by enhanced chemiluminescence luminosity (Amersham Pharmacia Biotech ...
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bioRxiv - Developmental Biology 2024Quote: ... They were then incubated overnight at 4°C with primary antibodies in blocking solution: rabbit anti-GFP (MBL Cat# 598; 1/300) and mouse anti-HA (Sigma, Cat# 12ca5; 1/50). On the next day ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were then co-labelled overnight at 4°C with primary antibodies directed against Paired Box 7 (Pax7; DSHB; 1:50) and Laminin α1 (Sigma-Aldrich #L9393; 1:100) in 2% BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... in PBS-T (1× PBS and 0.1% Tween 20) for 1 hour at room temperature and incubated overnight at 4°C with primary antibodies staining for FLAG (Sigma Cat# F1804, 1:500) and DDX50 (Proteintech Cat# 10358-I-AP ...
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bioRxiv - Biochemistry 2023Quote: ... The blots were probed with the following primary antibodies at room temperature for 1-2 hours or at 4 ℃ overnight: anti-GAPDH (Millipore, #MAB374, 1:5,000 dilution), anti-Flag M2 (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... Nitrocellulose membranes were blocked for 1 h with the blocking buffer containing 5 % dried milk in TBS-Tween 1 % and incubated over-night with primary antibodies: anti-αTubulin (Sigma, T6074, 1:1000 dilution), anti-acetylated Tubulin (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with primary antibody to stain tubulin (mouse anti-α-tubulin B-5-1-2 mAb, Sigma-Aldrich, T5168, dilution 1:250) for 4 h at room temperature ...
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bioRxiv - Genetics 2022Quote: ... 2% normal goat or donkey serum and 0.1% Triton X-100 in PBS (-) at room temperature for 1 h and reacted with mouse anti-parvalbumin monoclonal antibody (Sigma-Aldrich, P3088, 1:4000), goat anti-somatostatin antibody (Santa-Cruz ...
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bioRxiv - Immunology 2022Quote: ... and then incubated for 1 hour at 37°C with secondary goat anti-rabbit antibodies conjugated with fluorescein isothiocyanate (FITC; 1:250; Sigma-Aldrich, Prague, Czech Republic). Fluorescence intensity was measured using the Synergy H1 microplate reader (BioTek ...
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bioRxiv - Neuroscience 2023Quote: ... then washed three times in PBT and incubated in secondary antibody mix (goat anti-chicken Alexa Fluor 488, Jackson Immunoresearch #103-545-155, 1:200, goat anti-rabbit ATTO 647N, Sigma #40839, 1:100) for 24 - 48 hours in the dark while shaking at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 x 106 – 1 x 107 cell equivalents were analyzed on PAA / SDS gels and probed with anti-HA antibodies (Sigma, rat 3F10, 1:1000), anti-Myc antibodies (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... blocked with 5 % non-fat dry milk in TBST (TBS with 0.1 % Tween-20) and probed at 4 °C overnight with the following primary antibodies: mouse monoclonal anti-FLAG M2 (Sigma-Aldrich F3165, 1:1000), mouse monoclonal anti-α-tubulin B512 (Sigma-Aldrich T5168 ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were normalized to either actin or GAPDH housekeeping proteins with monoclonal antibodies (anti-actin; 1∶1000, Sigma or anti-GAPDH; 1:10,000 Abcam).
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bioRxiv - Microbiology 2024Quote: ... Fixed cells were washed twice with PBS + 0.1% Tween 20 and incubated with 1:1000 mouse anti-HCMV IE-1 monoclonal antibody (Millipore, Catalog No: MAB810R, clone 8B1.2) in staining buffer (Dulbecco’s PBS [DPBS] containing 1% FBS and 0.3% Triton X-100 ...
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bioRxiv - Cell Biology 2020Quote: ... Biopsies were digested in a buffer containing 10 mg collagenase II (C6885-1G, Sigma) and 100 mg BSA (A8806-5G ...
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bioRxiv - Developmental Biology 2022Quote: ... Mandibles were then pooled and incubated with 10 mg/ml Dispase II (Sigma-Aldrich) in HBSS supplemented with 10 µg/ml DNase (New England Biolabs ...
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bioRxiv - Bioengineering 2019Quote: ... and 0.5 mM of iron (II) chloride (FeCl3) (Sigma-Aldrich, cat no. 701122-1G). For E ...
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bioRxiv - Evolutionary Biology 2019Quote: ... cells were examined using ImageStream cytometry (ImageStream Mark II Imaging flow cytometer, EMD Millipore) with the following settings ...
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bioRxiv - Cell Biology 2019Quote: ... GW9662 and Akt I-II kinase inhibitor were purchased from Sigma-Aldrich (Madrid, Spain)
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bioRxiv - Biophysics 2020Quote: Epithelial cells from the Madin-Darby Canine Kidney cell line (MDCK II, Sigma #85011435) were maintained in polystyrene T75 flasks of in a cell culture incubator at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... the PTP inhibitor XXXI/II-B08 (PTPN11-inh.; Cat.#565852; EMD Millipore, Burlington, US) was purchased ...
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CRISPR-Cas9-mediated knockout of CYP79D1 and CYP79D2 in cassava attenuates toxic cyanogen productionbioRxiv - Plant Biology 2021Quote: ... Regenerated plants were maintained on MS2 in Phytatrays II (Sigma-Aldrich, St. Louis, MO), incubated at 28 °C in high light (90–150 μmol m-2 s-1 for 16 h light/8 h dark conditions ...
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bioRxiv - Cell Biology 2020Quote: ... and 0.00595% HEPES) supplemented with 0.4% collagenase II and 0.6% pancreatin (all from Sigma). Herein ...
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bioRxiv - Pathology 2022Quote: ... cells were plated in 4-well Nunc Lab Tek II chamber slides (Sigma-Aldrich) and fixed with 4% PFA for 10 min at RT ...
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bioRxiv - Neuroscience 2022Quote: ... 0.25% sodium deoxycholate with added protease and phosphatase inhibitor cocktail II (Sigma-Aldrich, Sweden). BCA protein assay kit (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were rinsed with distilled water and counterstained with Gill’s II Hematoxylin (Sigma, USA) to make the pia and superficial layers more visible and allow for pia thickness measurements ...
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bioRxiv - Molecular Biology 2020Quote: ... with stock solutions of 0.5 M ammonium iron(II) sulfate hexahydrate (Sigma-Aldrich, 09719), 0.5 M manganese(II ...
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bioRxiv - Cell Biology 2019Quote: ... Inhibition of Topoisomerase II was done using 10 µM of ICRF-193 (Merck-Millipore). Inhibition of histone deacetylases (HDACs ...
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bioRxiv - Microbiology 2020Quote: ... by using specific primers (supplementary Table II) according to the manufacturer instructions (Sigma-Aldrich).
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bioRxiv - Evolutionary Biology 2020Quote: ... and Dexamethasone at the above concentrations along with iPS Boost Supplements II (Millipore, SCM094). Two days later (5 days after induction) ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein expression for all constructs was conducted using Rosetta II pLac(I) cells (Millipore) grown to high density at 37 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were suspended in Stemline II Hematopoietic Stem Cell Expansion medium (Sigma-Aldrich S0192) supplemented with 1% peni/streptomycin and L-glutamine ...
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bioRxiv - Immunology 2021Quote: Anti-MHC-II was injected intravenously and Fingolimod/FTY720 (3 mg/kg, Sigma Aldrich) intraperitoneally the day before and just before SWAg footpad injection.
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bioRxiv - Bioengineering 2022Quote: ... and (ii) inlets of the hydrogel chamber were sealed using PCR tape (Sigma Aldrich). Depending on the type of analysis ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were resuspended in Stemline II Hematopoietic Stem Cell Expansion medium (Sigma-Aldrich S0192) supplemented with 1% peni-streptomycin and L-glutamine ...
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bioRxiv - Cell Biology 2022Quote: ... (ii) Pre-extraction solution: PEM buffer supplemented with 0.3% Triton X-100 (Sigma, 93443) and 0.125% Glutaraldehyde (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... Muscle lysates were further digested with 2 IU/ml of Dispase II (Sigma Aldrich) diluted in minimum amount of PBS for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... Y-27632 (10 µM) and Deoxyribonuclease II (10 U/ml, Sigma Aldrich #D8764-30KU), hereafter named ‘dissociation solution’ ...
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bioRxiv - Molecular Biology 2024Quote: ... Polymerase II inhibition was performed by adding 50 uM alpha-amanitin (Sigma-Aldrich, A2263) for either 6 hours prior to assaying cells or 1 hour prior to doxycycline addition ...
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bioRxiv - Molecular Biology 2024Quote: ... transfer it to a centrifuge tube and add digestion fluid (collagenase II(Sigma, USA)) ...
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bioRxiv - Neuroscience 2023Quote: ... 10 mM Na-β-glycerophosphate) containing phosphate inhibitor mixture I and II (Sigma-Aldrich) and complete protease inhibitor mixture (Roche ...