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Citations for Millipore Sigma :
5651 - 5700 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... PreScission-protease eluted supernatant was added to 100 µL of S-protein agarose (69704-3, EMD Millipore) to capture S-tagged protein ...
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bioRxiv - Cell Biology 2024Quote: ... UCCs were incubated with the corresponding retroviral supernatants containing 3 µg/ml Polybrene (Sigma-Aldrich, #H9268-106) for 72 h and subsequently selected in medium containing 200 µg/ml hygromycin or 1-2.5 µg/ml puromycin for about one week ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed 3×5mins with buffer A before incubation with PLA anti-rabbit PLUS (Millipore, DUO92002) and anti-mouse MINUS probes (Millipore ...
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bioRxiv - Cancer Biology 2024Quote: ... and 3 µM Alexa Fluor Dye 647 picolyl azide in Tris-Buffered Saline (TBS) (Sigma-Aldrich, T6066) (pH 8.3 ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were anesthetized using 168 µg/ml (w/v) of Tricaine (ethyl 3-aminobenzoate methanesulfonate) (E10521, Sigma) in ERM ...
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bioRxiv - Cell Biology 2024Quote: ... or chemically by incubating embryos for 3 minutes in 1.5 mg/mL Pronase E (Sigma-Aldrich, 107433) in Danieau’s 0.3X ...
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bioRxiv - Biochemistry 2020Quote: ... tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (34 μM, Sigma), copper(II ...
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bioRxiv - Biochemistry 2023Quote: ... tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (34 μM, Sigma), copper (II ...
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bioRxiv - Plant Biology 2020Quote: ... of internode above and below the node was selected for in vitro culture on the MS-0 medium with 3 gL−1 Phytagel (Sigma Chemical Co., St. Louis) and no plant growth regulator ...
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bioRxiv - Neuroscience 2020Quote: CSF samples were prepared for NMR by adding 16 µL 3-(Trimethylsilyl)-1-propanesulfonic acid-d6 sodium salt (DSS-D6; Chenomix/Sigma-Aldrich, Oakville, ON, Canada) to 30 µL CSF (pooled from 1 – 3 animals ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were further incubated at room temperature for 1 h with a blocking buffer (3% normal donkey serum; S30, Chemicon by EMD Millipore, Billerica, MA, USA) in PBS solution containing 0.2% Triton X-100 and 0.05% Tween 20 (PBST) ...
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bioRxiv - Biochemistry 2021Quote: ... Fixed cells were thoroughly washed with 1XPBS and then the cells were incubated in 3% BSA with 0.1% Triton X-100 in 1XPBS (Sigma, Cat. no. 9002-93-1) for permeabilization ...
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bioRxiv - Molecular Biology 2020Quote: ... 10× lentivirus concentrates were diluted in 1 ml of Opti-MEM containing 8 × 10−3 µg/ml of polybrene (Sigma Aldrich; St. Louis, MO) and incubated overnight at 37 °C ...
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bioRxiv - Genomics 2021Quote: ... the aliquot of sheared chromatin fragments were incubated with antibody conjugated Protein A Dynabeads using total histone 3- (unmodified H3 antibody, Millipore #05-499; 1:1000) or histone 4- (unmodified H4 antibody ...
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bioRxiv - Biochemistry 2022Quote: ... The parafilm was washed with water and blocked with 1x phosphate-buffered saline (PBS) containing 3% (w/v) BSA and then incubated in α-HA antibody (Sigma; 1:1.500 dilution) diluted in PBS buffer and 3% (w/v ...
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bioRxiv - Physiology 2022Quote: ... 3051.2) for 5 min and blocked in PBS containing 3% bovine serum albumin and 1% normal goat serum (Sigma-Aldrich, Taufkirchen, Germany, A4503 & G0023) at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... for 1-3 hours at room temperature (RT) and then permeabilized with 0.1% (v/v) Triton X-100 (Sigma-Aldrich, Cat # 9036-19-5) for 1 hour before blocking with 3% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... . Ultrathin sections placed on grids were stained with 1% aqueous uranyl acetate (Agar Scientific, Stansted, UK) and 3% lead citrate (Sigma Aldrich, St. Louis, USA), and observed at the FEI Morgagni 268D microscope.
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bioRxiv - Bioengineering 2024Quote: ... dissociated hiPSC-Cardiomyocytes and primary cardiac fibroblasts were mixed in a 3:1 ratio and resuspended in fibrinogen by mixing the cell solution with 33 mg/mL human fibrinogen (Sigma-Aldrich, cat. no. F3879) and RPMI-B27 (described above ...
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bioRxiv - Cell Biology 2023Quote: ... the cell lines were treated in triplicate with a top dose of 10uM in 1:3 dilutions for a total of 12 doses with paclitaxel (Sigma-Aldrich Product Number T7191), fulvestrant (Sigma-Aldrich Product Number I4409) ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 3 units/ml of human EPO (Peprotec, #100-64) and 10 ng/ml of G-CSF (Filgrastim, Millipore Sigma, 121181-53-1) (Stem Cell Technologies ...
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bioRxiv - Plant Biology 2023Quote: ... in a petri dish and then placed in 12-well plates containing 90% liquid MS medium (1/4 strength) and 10% polyethylene glycol (PEG, MW 8000, SIGMA, cat # 25322-68-3) (p/v ...
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bioRxiv - Microbiology 2021Quote: Metabolic function was evaluated using a (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay from Sigma [37] ...
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bioRxiv - Biophysics 2022Quote: ... We dissolve 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methyl-morpholinium chloride (DMTMM) powder (Sigma Aldrich) in HEPES buffer (pH 7.18 ...
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bioRxiv - Molecular Biology 2023Quote: ... and the coupling reagent 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium (DMTMM) chloride (Sigma-Aldrich). Stock solutions were prepared in phosphate-buffered saline at concentrations of 140 mM (PDH ...
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bioRxiv - Microbiology 2024Quote: ... CPSs were incubated with 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM, Sigma-Aldrich). The mixture was then ultrafiltered to remove extra DMTMM and mixed with carboxyl latex beads (2 μm ...
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bioRxiv - Neuroscience 2020Quote: PF-4778574 [N-<(3R,4S)-3-[4-(5-cyano-2-thienyl)phenyl]tetrahydro-2H-pyran-4-yl>propane-2-sulfonamide] was obtained as a powder from Sigma (catalog #PZ0211) and solubilized (2.5 mg/ml ...
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bioRxiv - Biophysics 2024Quote: ... Cells were washed with PBS and incubated with 100 µM 2-Deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl) amino]-D-glucose (2NBDG) (Sigma Cat# 186689-07-6) containing RPMI1640 (HiMedia Cat# AT150 ...
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bioRxiv - Microbiology 2020Quote: ... The sample was diluted and purified with a 3 kDa Amicon Ultra centrifugal filter (Millipore, Temecula, CA, USA) to remove low molecular weight impurities ...
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bioRxiv - Microbiology 2020Quote: ... plates supplemented with 3% sheep blood (iTek StPaul MN) and 20 μg/mL neomycin (Sigma St Louis MO) and then grown overnight at 37°C in a 5% CO2 humidified incubator ...
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bioRxiv - Biophysics 2020Quote: ... the cells were fixed in 3% formaldehyde solution (37 wt.% in H2O, 252549, Sigma-Aldrich, St. Louis, MO) with 0.1% glutaraldehyde solution (25% in H2O ...
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bioRxiv - Cancer Biology 2021Quote: ... The cell lines were regularly passaged every 3-4 days by dissociation with Trypsin-EDTA 0.25% (Sigma Aldrich). The cell lines were regularly subjected to monthly screening for mycoplasma infection ...
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bioRxiv - Immunology 2021Quote: ... After 3 washes in TBST the blot was revealed using the EC™ Prime Western Blotting System (Sigma). The membrane was imaged using a Chemidoc MP imaging system from BioRad.
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bioRxiv - Developmental Biology 2021Quote: ... Alive embryos older than 16 ss were additionally mounted with 0.016% ethyl 3-aminobenzoate methanesulfonate salt (Tricaine, Sigma) in the LMA and added to the E3 medium to prevent movement during imaging ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed twice with 3% BSA in PBS 1X and stained with 5μg/mL Hoechst (Sigma Aldrich). Samples’ images were acquired and analysed by high content fluorescence microscope Operetta® (PerkinElmer ...
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bioRxiv - Molecular Biology 2021Quote: A2780-DNA329 and OVCAR-3 (ATCC) ovarian cancer cell lines were cultured in RPMI-1640 medium (R8758, Sigma). Ovarian cancer COV362 (ECACC) ...
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bioRxiv - Cell Biology 2021Quote: ... washed 3 times in 1X PBS and incubated for 5 min in 50mM ammonium chloride (Sigma-Aldrich, 254134) before mounting on cavity blades (Marienfeld ...
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bioRxiv - Neuroscience 2021Quote: ... blocking and permeabilization was performed with DPBS + 3% BSA + 0.1% Triton X-100 (Millipore Sigma cat. no. X100) for 30 min at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... Degradation of AID-tagged CHD4 was induced by the addition of 500 μM 3-Indoleacetic acid (I2886, Sigma).
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bioRxiv - Molecular Biology 2021Quote: ... Denmark) as well as the BKCa blockers paxilline (3 μmol/L with 0.03% DMSO, Sigma-Aldrich, Darmstadt, Germany) and iberiotoxin (100 nmol/L ...
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bioRxiv - Immunology 2021Quote: ... The detached peptides were separated from HLA molecules using 3 kDa cut-off centrifugal ultrafilters (Millipore, MO, USA), and then concentrated using vacuum centrifugation.
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bioRxiv - Molecular Biology 2020Quote: ... The eluted nsp7 sample was concentrated with a 3 kDa molecular weight cut-off Centrifuge Filter (Millipore Corporation) and then size-separated by a Superdex S75 10/300 GL column in 25 mM HEPES pH 7.4 ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were split into 3 replicates containing 6.6 million cells each and treated with either DMSO (Sigma-Aldrich) or 40nM erlotinib (Selleckchem) ...
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bioRxiv - Bioengineering 2020Quote: ... Fixed collagen samples were washed 3 times with a solution containing 50 mM PIPES pH 7.4 (Sigma, P6757) kept in ice and then they incubated for 2h in ice and in the dark in a freshly prepared staining solution (SSI ...
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bioRxiv - Cancer Biology 2020Quote: ... Mo and PN were then washed in DMEM and placed in 3 μm Boyden chambers (Merck Millipore, France), at the concentration of 0.5 x 106 cells/chamber in 200 µl DMEM ...
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bioRxiv - Physiology 2020Quote: ... Each experimental condition was plated in triplicate and 3 wells were always treated with fulvestrant (ICI182,240) (Sigma Aldrich) in order to measure the basal ERα transcriptional activity ...
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bioRxiv - Microbiology 2020Quote: ... Fractions containing the protein were pooled and concentrated using an Amicon with a 3 KDa cut-off (Millipore). The protein was conserved in 10 mM Tris-HCl pH7.63 and 33% glycerol ...
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bioRxiv - Biophysics 2020Quote: ... Cleaned glass coverslips were then treated during one hour with 3-(trimethoxysilyl)propyl acrylate (Sigma #2530-85-0) to promote a strong adhesion between the hydroxy-PAAm hydrogel and the glass coverslips and finally dried under a nitrogen flow ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant (total protein concentration ∼3 mg/ml) was incubated with ANTI-FLAG M2 agarose beads (Sigma Aldrich) at 4°C for 1 h with agitation ...
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bioRxiv - Bioengineering 2021Quote: ... then rinsed 3 times with PBS and permeabilized with staining buffer (0.1% Triton X-100/PBS, Sigma, 93443) for 1 hour before immunolabeling.