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Citations for Millipore Sigma :
501 - 550 of 2403 citations for rno mir 155 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: Primers and DNAzymes were purchased from Sigma Aldrich. Purified S ...
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bioRxiv - Molecular Biology 2021Quote: ... and DuetDOWN1 primers (Novagen Cat. No. 71179-3), as well as the original primers ...
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bioRxiv - Cell Biology 2020Quote: ... E@sy Oligo primers (Millipore-Sigma, Burlington, MA) or TaqMan Assays (Life Technology ...
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bioRxiv - Neuroscience 2020Quote: ... 1 μl of each 10 μM primer (Sigma), 2μl of 10X PCR buffer (Qiagen 203203) ...
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bioRxiv - Immunology 2021Quote: ... 0.5 μg primer for cDNA synthesis (Sigma Aldrich) and RNasin inhibitor (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... long primers (90 bp in total, Sigma-Aldrich) were used ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Oligonucleotides (DNA primers) were obtained from Sigma Aldrich unless specified otherwise and are mentioned in Supplementary Table 1.
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bioRxiv - Molecular Biology 2023Quote: The desalted primers were synthesized by Sigma-Aldrich chemicals Pvt ...
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bioRxiv - Cell Biology 2023Quote: ... All the primers were synthesized from Sigma-Aldrich. Barcode Biosciences ...
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bioRxiv - Immunology 2023Quote: We obtained the AMP primers from Sigma-Aldrich Ltd ...
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bioRxiv - Microbiology 2023Quote: ... primers listed in Table S3 (Sigma, Bangalore, India) were designed to amplify the kanamycin cassette from the template ...
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bioRxiv - Genomics 2023Quote: ... Primers were diluted in Nuclease-free water (Sigma) to use in a final concentration of 10 µM.
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bioRxiv - Cell Biology 2022Quote: ... with 150nM Forward and Reverse primers (Sigma-Aldrich; Supplementary Table 5 for primer sequences) ...
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bioRxiv - Cell Biology 2022Quote: ... Primers used for SYBR Green assays (Sigma-Aldrich) can be found in Table S4.
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bioRxiv - Microbiology 2023Quote: ... and the Uni12 primer (5’-AGCAAAAGCAGG-3’, Sigma). For mRNA analysis Oligo(dT ...
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bioRxiv - Neuroscience 2023Quote: ... 0.5 μl of each primer (25 μM; Sigma), and 4 μl of 1:20 diluted lysed tail DNA in a final volume of 25 µl using dH2O ...
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bioRxiv - Physiology 2024Quote: ... The primers were all purchased from Millipore Sigma with sequences listed as below ...
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bioRxiv - Microbiology 2024Quote: ... Using gene-specific primers purchased from Sigma-Aldrich, the PCR experiment was carried out employing the temperature profile shown in Table S4 ...
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bioRxiv - Neuroscience 2024Quote: ... and the following gene-specific primers (Sigma-Aldrich): csf1 (NM_007778.4 ...
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bioRxiv - Microbiology 2024Quote: ... antibiotics and primers were purchased from Sigma-Aldrich. Restriction and cloning enzymes were obtained from New England Biolabs (NEB) ...
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bioRxiv - Immunology 2024Quote: ... 1 µL i7 Index primer (Sigma-Aldrich®), and 1 µL i5 Index primer (IDT ...
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bioRxiv - Physiology 2020Quote: ... miR-150fl/Cdh5(PAC)-iCreERT2 mice were injected intraperitoneally with 100 µL of 5 mg/mL tamoxifen (Sigma, cat. no. T5648) or vehicle (12.5% vol/vol ethanol in peanut oil ...
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bioRxiv - Cancer Biology 2019Quote: ... mice were treated via tail vein injection with 80 μg of control miRNA or 5-FdU-miR-15a packaged using in vivo-jetPEI (Polyplus Transfection, Illkirch, France) or with 50 mg/kg of gemcitabine (Sigma Aldrich) via intraperitoneal injection (IP injection) ...
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bioRxiv - Molecular Biology 2023Quote: ... with either the wild type Cacna2d2-3’UTR or the Cacna2d2 3’UTR bearing mutations in the miR-423-5p binding sites (Cacna2d2 mut 3’UTR) in HEK 293 cells (Millipore Sigma). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA for reverse transcription polymerase chain reaction (RT-PCR) was isolated from 100 mg of oat leaves using TRI REAGENT® (Sigma-Aldrich, St. Louis, MO, USA) according to the provided protocol ...
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bioRxiv - Biochemistry 2020Quote: ... 10 mM βME and protease inhibitor (Protease Inhibitor Cocktail Set III, Sigma)) and sonicated on ice for 1 minute 30 seconds (15s ON ...
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bioRxiv - Cell Biology 2021Quote: ... PLA was performed using Duolink in situ Starter Set GREEN (Sigma-Aldrich) according to manufacturer’s instructions using rabbit anti-Myc polyclonal antibody and mouse anti-Flag monoclonal antibody.
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bioRxiv - Neuroscience 2020Quote: ... nAChRα5 and nAChRα6 DNA oligonucleotide sets were synthesised by Sigma-Aldrich (Merck) and enzymatically labelled with ATTO-633 according to (Gaspar et al. ...
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bioRxiv - Neuroscience 2019Quote: ... In the set of experiments involving rapamycin (200 nM, Sigma-Aldrich, Germany) slices were let to recover in rapamycin-infused aCSF or DMSO (control group ...
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bioRxiv - Biochemistry 2022Quote: ... 10 mM βME and protease inhibitor (Protease Inhibitor Cocktail Set III, Sigma)) and sonicated on ice for 1 minute 30 seconds (15s ON ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented with 1:200 Protease Inhibitor Cocktail Set III (#539134, Merck Millipore)) ...
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bioRxiv - Biophysics 2019Quote: ... set on Millicell Cell Culture Inserts (Millipore, 0.4 mm; 30 mm diameter), and cultured for up to 14 days on multiwell-plates at 35°C/5% CO2 in a culture medium composed of 50% Basal Medium Eagle ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue sets were stored either in PBS with 0.1% sodium azide (Sigma) at 4°C or in cryoprotectant comprised of glycerol ...
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bioRxiv - Physiology 2022Quote: ... tissues were homogenized using a KIMBLE Dounce tissue grinder set (Sigma Aldrich, catalog no.
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bioRxiv - Physiology 2024Quote: ... and phosphatase inhibitor cocktail set II (Millipore, 524625-1set, diluted 1:100) using an automatic homogeniser (VWR ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.5% (v/v) Phosphatase Inhibitor Cocktail Set V (Millipore Sigma, 524629). The resulting lysates were centrifuged to collect supernatant for downstream Western Blotting ...
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bioRxiv - Physiology 2019Quote: ... 0.25 μL of each of the two matched primers (one forward and one reverse primer for longer amplicon or one forward and one reverse primer for shorter amplicon) (Sigma-Aldrich), 1 μL of a FAM-labeled MT-ATP 8-probe or 1 μL of a MVIC-labeled GAPDH-probe (both probes from Applied Biosystems ...
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bioRxiv - Systems Biology 2021Quote: ... Three reference genes were selected for gene expression analysis: TBP, HPRT1, and GAPDH (Allen et al, 2008).) All primers were KiCqStart primers designed by and purchased from Sigma Aldrich. Ct values and PCR amplification efficiencies were calculated using LinRegPCR software (version 2017.1 ...
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bioRxiv - Molecular Biology 2020Quote: Primer sequences for markers were obtained from the UCSC genome browser and standard HEX-tagged primers (Sigma-Aldrich, Supplementary Table 2) were used to assess the flanking haplotypes of MMP20 ...
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bioRxiv - Cell Biology 2020Quote: ... Expression of genes of interest was measured using primers designed with the NCBI Primer designing tool and custom-synthesized by Sigma-Aldrich. We list primers used in the present study (Table S9) ...
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bioRxiv - Synthetic Biology 2022Quote: ... based plasmid encoding GR1-CFP was generated in a prior study.21 Three forward primers and one reverse primer were synthesized using the 22c-trick (Sigma-Aldrich) to generate each SSM library as previously described (Table S2).21,59 E ...
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bioRxiv - Genetics 2021Quote: All ORF-specific PCR primer sequences used in this validation are shown in Additional file 1: Table S3 and primers were obtained from Millipore Sigma unless mentioned otherwise from Integrated DNA Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... Sections were washed two times with PBS and incubated for two hours at RT with corresponding secondary antibodies and subsequently incubated for 30 min at RT with DAPI (Sigma). Stained sections were washed two times with PBS and mounted with either Fluorsave (Chemicon ...
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bioRxiv - Microbiology 2020Quote: ... RT SigmaFast O-phenylenediamine (OPD) substrate (P9187, Sigma) was added to the wells after six additional washes to develop the colorimetric reaction ...
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bioRxiv - Physiology 2021Quote: ... For the RT-qPCR the Kappa Mix (Sigma) was used and the primers concentration was 10 µM ...
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bioRxiv - Cell Biology 2023Quote: ... permeabilized at RT with 0.25% TritonX100 (Sigma #T9284) for 10 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... and 50 mg Fast Blue RT (Sigma-Aldrich) in 100 ml of 50 mM 2-amino-2-methyl-1,3-propanediol buffer (pH 9.8) ...
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bioRxiv - Cancer Biology 2021Quote: RIP assays were conducted to explore the relationship between lncRNA NEAT1 and miR-1299 via the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, MA, USA). Initially ...
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bioRxiv - Microbiology 2022Quote: ... and oligo(dT) primers (Eurofins Genomics) for detection of mRNA or a fluA uni12 forward primer [92] (Sigma-Aldrich, 5’-AGCAAAAGCAGG-3’) to detect vRNA according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and treated with DNAse (On-Column DNase I Digestion set, Sigma, Inc., USA). cDNA libraries were prepared with the TruSeq RNA sample Preparation v2 kit (Illumina Inc. ...