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Citations for Millipore Sigma :
501 - 550 of 4804 citations for Dnp pro leu gly cys me his ala D arg nh2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The iPSC clusters were picked in 16mM glucose containing RPMI-1640 medium lacking Cys/Met (Cat. No. R7513, Sigma-Aldrich), washed twice with the same medium and pulse labeled for 15 min with 35S-Cys/Met (300 μCi ...
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bioRxiv - Cell Biology 2021Quote: ... and C-terminal (YAPGTGGGATI-NH2, 1.5 mg) regions of proGATI were individually conjugated to keyhole limpet hemocyanin (KLH; 3 mg; Sigma H-7017, Lot 110K4833). An additional Cys residue added to the N-ter peptide allowed conjugation to KLH using m-maleimidobenzoyl-N-hydroxysuccinimide ester ...
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bioRxiv - Immunology 2024Quote: TFH cells (PD-1hiCXCR5hi) were generated by immunizing NOD mice intraperitoneally with KLH (keyhole limpet hemocyanin) or KLH-DNP (Sigma-Aldrich, St. Louis, MO, USA) 3 times (100 µg/dose ...
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bioRxiv - Biophysics 2021Quote: ... D-(+)-Glucose (D-Glu; Sigma-Aldrich, 20 mM) or L-(–)-Glucose (L-Glu ...
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bioRxiv - Systems Biology 2021Quote: ... plus D-glucose (EMD) and D-galactose (Sigma). Cultures were grown in a humidified incubator (Infors Multitron ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... D-mannitol and D-trehalose dihydrate (Sigma-Aldrich) at a concentration between 50 and 500 mg/mL were prepared by simple mixing (Table 1) ...
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bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
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bioRxiv - Genetics 2020Quote: ... Pro-inflammatory stimulation with LPS (Sigma, #055:B5, 0.5 μg/ml) and IFNγ (PeproTech ...
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bioRxiv - Developmental Biology 2022Quote: ... polyclonal rabbit anti-pro surfactant protein C (1:4000, Millipore, #AB3786), mouse monoclonal acetylated tubulin (Sigma ...
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bioRxiv - Immunology 2021Quote: ... and the following antibodies: anti-mouse pro-SPC (AB3786, EMD Millipore), anti-mouse MHCII (107601 ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-pro-surfactant protein C (SFTPC, 1:1000, AB3786, Millipore), goat anti-SOX9 (SOX9 ...
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bioRxiv - Immunology 2020Quote: ... 55μM 2-mercaptoethanol (2-ME) supplemented with 50 U/ml Benzonase (Sigma). Aliquots derived from the same donors were combined and all samples were enriched for T cells by removing CD14 and CD19 expressing cells using a column-based magnetic depletion approach according to the manufacturer’s recommendations (Miltenyi) ...
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bioRxiv - Neuroscience 2020Quote: ... in 0.2 M MES buffer (pH6.5) with or without Thiorphan (Sigma T6031) for 1 hour at 37°C ...
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bioRxiv - Immunology 2022Quote: ... 5% b-ME) supplemented with protease and phosphatase inhibitors (Roche/Sigma-Aldrich). A Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... in 100 mM 2-(N-morpholino)ethanesulfonic acid (MES; Sigma-Aldrich #M1317) buffer (pH 6.5 ...
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bioRxiv - Genomics 2024Quote: ... and 19 (clones: C-11, PCK-26, CY-90, KS-1A3, M20, A53-B/A2, C2562, Sigma, St. Louis, MO, USA), mouse IgG1 anti-human CK 19 (clone ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were cultured and passaged every 2 or 3 days in Dulbecco’s modified Eagle’s medium (DMEM) (Hi Media Laboratories) supplemented with 10% FBS (Hi Media Laboratories) and 1% penicillin/streptomycin (Sigma-Aldrich, St. Louis, MO, USA). Both cell types were cultured at 37°C and in a humidified incubator with a 5% CO2 atmosphere ...
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bioRxiv - Immunology 2021Quote: ... This was followed by 0.5ug/mL anti-his HRP (Sigma), and binding was detected after incubation with substrate at an absorbance of 450nm ...
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bioRxiv - Biochemistry 2020Quote: ... The antibodies used for western blotting were anti-His (Sigma), anti-Strep HRP conjugated (iba ...
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bioRxiv - Biochemistry 2020Quote: ... FLAG tag (F1804) or His tag (H1029) were from Sigma. Mouse monoclonal antibody raised against hemagglutinin (HA ...
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bioRxiv - Plant Biology 2020Quote: ... and 1:5000 dilution of primary anti-His (SIGMA, USA), 1:10000 for anti-GST (SIGMA ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 ng recombinant His-AKT1 (inactive, Upstate/Millipore #14-279), at a final volume of 30 μl ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were detected using α -His from mouse (Sigma H1029), α-Gfp from mouse ...
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bioRxiv - Biochemistry 2020Quote: Recombinant Ded1-His was expressed from the pET22b plasmid (Novagen) and purified as previously described (46) ...
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bioRxiv - Genetics 2021Quote: ... His-TCF7 was PCR cloned into pET52b (Millipore, 71554-3) using the primers 5’-CGGGGTACCTCATGCCGCAGCTGGACTCC-3’ and 5’-ATAGTTTAGCGGCCGCGAGCACTGTCATCGGAAGGAAC-3’ ...
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bioRxiv - Biochemistry 2021Quote: α-PCNA (P8825) and α-HIS (H1029) were purchased from Sigma; α-Cul2 (EPR3104 ...
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bioRxiv - Microbiology 2023Quote: N-terminal His-tagged KtrD cloned into pRSFDuet-1 (Novagen) was overexpressed in Escherichia coli BL21 (DE3 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Anti-His primary antibody (H1029) was purchased from Sigma Aldrich, HRP conjugated secondary antibody (J1430 ...
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bioRxiv - Biochemistry 2023Quote: ... 25 uL of (His)6-tagged TEV-protease (Sigma-Aldrich) was added and the solution incubated overnight at 4°C.
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bioRxiv - Cancer Biology 2023Quote: ... which were cryopreserved in HI FBS with 10% DMSO (Sigma). Cells were directly transferred to a -80 °C freezer in Coolcell freezing containers (Corning ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3% heat-inactivated fetal bovine serum (HI-FBS; Sigma) at 37°C for 15 ∼ 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... One milliliter of equilibrated HIS-Select Nickel Affinity Gel (Sigma) was added to the cleared cell lysate and incubated with mild agitation for 1 h at 4°C ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% HI-horse serum and vancomycin (Sigma, USA). The inoculated flask was then kept in shaking conditions (100 rpm ...
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bioRxiv - Biochemistry 2023Quote: ... and BRAF NTs/ BRAF KD with His antibody (Sigma SAB5600227). Finally ...
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bioRxiv - Genetics 2022Quote: ... and detected with His and GST antibodies (Sigma-Aldrich, USA), respectively.
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bioRxiv - Cancer Biology 2024Quote: ... 2% heat-inactivated Fetal Bovine Serum (HI-FBS; Sigma-Aldrich), 1% v/v penicillin-Streptomycin (VWR) ...
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bioRxiv - Cancer Biology 2024Quote: ... 10% glycerol) supplemented with His-tag protease inhibitors (Sigma-Aldrich). Samples were sonicated twice using a MSE Soniprep 150 apparatus (Sanyo ...
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bioRxiv - Immunology 2022Quote: ... Non-specific binding sites were blocked using fluorescence-activated cell sorting (FACS) buffer (1× PBS, 2% hi-FBS) supplemented with 20% hi-FBS and 10 μg mouse IgG (Sigma-Aldrich, St-Louis, MO, USA). Cells were stained using the following conjugated mouse anti-human monoclonal antibodies (mAbs) ...
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bioRxiv - Immunology 2021Quote: ... Cy3-STxB was generated by conjugating Cy3 dye onto STxB according to the manufacture manual of CY®3 Ab Kit (PA330000, Sigma-Aldrich).
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bioRxiv - Cell Biology 2020Quote: ... and incubated with Cy-3-conjugated goat anti-rabbit IgG as the secondary antibody (1:500, Cat # AP132C, Millipore Sigma, Burlington, MA) at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... 200nM actinomycin D (act D) (Sigma-Aldrich, St. Louis, MO), an equivalent volume of dimethyl sulfoxide (DMSO ...
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bioRxiv - Immunology 2020Quote: ... [12C]D-d7-glucose(2g/L) (97% D, Sigma-Aldrich) in 100% D2O ...
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bioRxiv - Developmental Biology 2023Quote: ... 2-Deoxy-D-glucose (2DG, Sigma-Aldrich, #D-8375-1G) 1M ...
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bioRxiv - Bioengineering 2023Quote: ... D-(–)-2-Amino-5-phosphonopentanoic Acid (D-APV, Sigma-Aldrich) 80 µM ...
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bioRxiv - Cancer Biology 2020Quote: ... The pre-pro-B-BCR/ABLp185-GFP were cultured in RPMI (Sigma) with 10% FCS and P/S57.
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bioRxiv - Biophysics 2023Quote: ... propidium iodide (Pro) at a concentration of 20 μM (Sigma-Aldrich, P4170). The incubation process was carried out in the dark at room temperature for a duration of 15 minutes ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... AT2 cell marker (rabbit Pro-SPC, 1:200 dilution, Merck Millipore, 3786) or AM marker (rabbit CD11c ...
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bioRxiv - Developmental Biology 2022Quote: ... Anti-Prosurfactant Protein C (pro-SP-C) antibody (1:4000) (Millipore, #AB3786). Antibodies were incubated in 2% powder milk in TBS pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... yeast cells were pre-cultured for 24h in SD–URA at 30°C and then shifted to SD-LEU medium supplemented with 1 µM β-Estradiol (Sigma- Aldrich, St. Louis, MO), incubated for 16-18h at room temperature ...
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bioRxiv - Genetics 2023Quote: ... followed by inoculation into SC-Leu medium supplemented with 100 mM or 25 mM KCl using a 48-pin replica plater (Sigma-Aldrich, St. Louis, MO, USA). Plates were incubated at 30°C and imaged after two days with the Bio-Rad ChemiDoc XRS+ imager ...