Labshake search
Citations for Millipore Sigma :
451 - 500 of 4804 citations for Dnp pro leu gly cys me his ala D arg nh2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... Mouse anti-His antibody (1:12000) (Sigma-Aldrich) and biotinylated PNA (1:6000 ...
-
bioRxiv - Biophysics 2020Quote: ... His-purifying resin was from Novagen (Darmstadt, Germany). Bis-(sulfosuccinimidyl ...
-
bioRxiv - Cell Biology 2021Quote: ... and Anti-His(1:3000) antibody(Sigma #H1029).HRP conjugated mouse IgG was used at 1:4000 dilution(Sigma #A3673 ...
-
bioRxiv - Biochemistry 2021Quote: ... anti-His (Sigma H1029; 1:3000 for IB), anti-Hsp70 (Abcam ab47455 ...
-
bioRxiv - Plant Biology 2022Quote: ... followed by blotting using Anti-His (SAB4301134, Sigma) and Anti-GST antibody (ab9085 ...
-
bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
-
bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
-
bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 2% GlutaMAX (200 mM ...
-
bioRxiv - Cell Biology 2024Quote: ... +10% HI FBS(Sigma Aldrich, cat#F4135-500ML) + penicillin/streptomycin (Sigma ...
-
bioRxiv - Genomics 2023Quote: ... with 10% heat-inactivated (HI) FBS (Sigma F0926). Vero/hSLAM cells were a kind gift from Dr ...
-
bioRxiv - Systems Biology 2023Quote: ... MCF7 in DMEM-Hi Glucose medium (Sigma-Aldrich) HCC1937 and ZR-75-1 in RPMI 1640 media (Gibco) ...
-
bioRxiv - Immunology 2023Quote: ... purified human neutrophil or mouse BM-derived neutrophils were stimulated with 10-7M N-formyl-Met-Leu-Phe (fMLF; Sigma-Aldrich) or 1 μM WKYMV (Phoenix Pharmaceuticals ...
-
Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populationsbioRxiv - Evolutionary Biology 2020Quote: ... We spotted the entire volume of each of the other 88 wells on CSM-Arg (Sunrise Scientific) plates supplemented with 100 mg/L L-canavanine (Sigma-Aldrich, St. Louis). Plates were allowed to dry overnight at room temperature ...
-
bioRxiv - Microbiology 2021Quote: ... and extensively dialysed against HEPES-buffered saline and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag mAb, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (Bethyl).
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... thereafter referred to as Mpp75Aa1.1-His) or without the C-terminal His tag (thereafter referred as Mpp75Aa1.1) was cloned into a pET24a vector (Novagen, Madison, WI) and expressed in Rosetta 2 (DE3 ...
-
bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
-
bioRxiv - Immunology 2023Quote: ... All antibodies were incubated separately at a concentration of 10 μg/ml in tubes together with 330 ng/ml HIS-tagged CD40 recombinant protein and 4 μg/ml peroxidase-coupled anti-HIS detection antibody (Sigma-Aldrich) for 60 minutes in ELISA buffer (PBS ...
-
bioRxiv - Plant Biology 2024Quote: 5 μg kinase OsDMI3-GST was incubated with 5 μg substrates (OsPrx20-His, Os Prx20T244A-His, or myelin basic protein [MBP; Sigma-Aldrich]) in kinase reaction solution for 30 min as described previously (Shen et al. ...
-
bioRxiv - Biophysics 2020Quote: TCRβ-reactive H57 antibody single chain fragments featuring a C-terminal unpaired cysteine residue (H57 cys scFV) were expressed in E.coli BL-21 as inclusion bodies using the pET expression system (Novagen) and refolded as described (9 ...
-
bioRxiv - Molecular Biology 2023Quote: ... incubated with a poly-dT oligonucleotide coupled with Cy-2 and a murin anti-FUS primary antibody (Sigma Aldrich) as previously described.
-
bioRxiv - Biophysics 2024Quote: Red-fluorescent carboxy-coated polystyrene beads (diameter = 100 nm, InvitrogenTM FluoSpheresTM F8801) were passivated with amine-terminated methoxy-PEG (mPEG-NH2; MW 750 Da; Millipore Sigma 07964) following the protocol described by Valentine ...
-
bioRxiv - Systems Biology 2021Quote: ... D-maltose or D-glucose (Sigma Aldrich). The bioreactors were inoculated with 100 mL of yeast shake flask cultures ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... D-(+)-Glucose (D-gluc, Sigma-Aldrich #49152), Eucalyptol (euc ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... D-(+)-Galactose (D-gal, Sigma-Aldrich #G6404), D-(+)-Glucose (D-gluc ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... D-serine (D-ser, Sigma-Aldrich #S4250), D-leucine (D-leu ...
-
bioRxiv - Pathology 2024Quote: ... The membranes were blocked with 3% bovine serum albumin in 0.5% Tween-20/Tris-buffered saline (TTBS) for 1 h and incubated with an anti-2,4-dinitrophenylhydrazone (DNP) adducts polyclonal antibody (1:100, EMD Millipore, Billerica, MA, USA, #MAB2223) or HNE polyclonal antibody (1:2000 ...
-
bioRxiv - Immunology 2024Quote: ... 2-deoxyglucose (2-DG, ref. D8375), 2,4-dinitrophenol (DNP, ref. D198501) and rotenone (ref. R8875) was purchased from Sigma-Aldrich (Saint-Louis, MO, USA). Poly(I:C ...
-
bioRxiv - Biochemistry 2020Quote: ... The cells were restreaked onto SD/-Trp/-Leu and incubated at 30 °C for 2–3 days before performing a colony-lift β-galactosidase assay (X-gal, Sigma-Aldrich) according to the Yeast Protocols Handbook (PT3024-1 ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... We diluted the saturated culture 1:10 in 96-well plates containing 200 μL of SC[MSG] -ura -leu containing Estradiol (β-estradiol, Sigma-Aldrich) at different concentrations (0 ...
-
bioRxiv - Biochemistry 2024Quote: The activity of the enzyme was estimated by measuring the rate of hydrolysis of the fluorescent substrate L-leucine-7-amido-4-methyl coumarin (Leu-AMC, Sigma–Aldrich, L2145) followed at 460 nm (excitation at 380 nm ...
-
bioRxiv - Systems Biology 2024Quote: - induction of tagging twice on SC-Leu Raf/Gal plates (20 g/l galactose (Serva, 22020) and 20 g/l raffinose (Sigma-Aldrich, R0250) instead of glucose) ...
-
bioRxiv - Systems Biology 2024Quote: - induction of tagging twice on SC-Leu Raf/Gal plates (20 g/l galactose (Serva, 22020) and 20 g/l raffinose (Sigma-Aldrich, R0250) instead of glucose) ...
-
bioRxiv - Neuroscience 2021Quote: ... and then incubated with TO-PRO-3 iodide (T3605, Sigma) for 30 min at RT ...
-
bioRxiv - Cell Biology 2021Quote: ... Sftpc (1:100, anti Pro Surfactant protein, rabbit, AB3786 Millipore), Ki67 (1:200 ...
-
bioRxiv - Developmental Biology 2022Quote: ... rabbit anti-pro-surfactant protein C (Sigma, Ab3786; 1:2000), rabbit anti-TGFβ2 (Proteintech ...
-
bioRxiv - Molecular Biology 2023Quote: ... rabbit anti-pro-Surfactant C (AB3786, Millipore; 1:1500 dilution), and for one hour at room temperature with the following fluorescent secondary antibodies ...
-
bioRxiv - Cell Biology 2024Quote: ... and incubated with 10 µg/mL Pro K (Sigma Aldrich) for 1 h at 37 °C as detailed before [27] ...
-
bioRxiv - Biophysics 2020Quote: ... the soluble His-tagged proteins were produced and purified after binding to a Ni-NTA His-Bind resin (Sigma-Aldrich, MO, US) as described by Martínez-Martínez (19) ...
-
bioRxiv - Plant Biology 2020Quote: ... 0.25 g/L 2-(N-morpholino)ethanesulfonic acid -MES-(Sigma Aldrich), pH 5.7) ...
-
bioRxiv - Plant Biology 2021Quote: ABA (Duchefa Biochemie) was dissolved in a 10mM MES (Sigma-Aldrich) buffer to make a 25mM ABA master mix (pH 5.8) ...
-
bioRxiv - Biochemistry 2023Quote: ... 20 mM KOH- MES (pH6.0)] with 10 μM BKA (Sigma B6179) for 15 min on ice as reported previously 35 ...
-
bioRxiv - Immunology 2024Quote: ... and 20 mM MES hydrate (CAS 1266615-59-1, Sigma-Aldrich). The pH was subsequently adjusted to the desired levels at 37 °C by addition of 1 M NaOH ...
-
bioRxiv - Developmental Biology 2024Quote: ... 0.5g/L MES 2-(MN-morpholino)-ethane sulfonic acid (Sigma M2933), 0.8% Bacto Agar (BD ...
-
bioRxiv - Biophysics 2020Quote: ... The (His)8-tag was cleaved by thrombin (Sigma) using 20 U per 1 mg GltPh in the presence of 5 mM CaCl2 at room temperature overnight ...
-
bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
-
bioRxiv - Biochemistry 2020Quote: ... and quantitative immunoblotting using an anti-His antibody (Novagen).
-
bioRxiv - Cell Biology 2022Quote: ... mouse α-His-HRP (A7058, Sigma-Aldrich, 1:10000).
-
bioRxiv - Biochemistry 2021Quote: ... recombinant His-Akt1 was from EMD Millipore (#14-279); recombinant GST-mTORf (containing a 30 fragment of mTOR encoding amino acids 2144-2175 ...
-
bioRxiv - Biophysics 2022Quote: ... Anti-His mAb was from Sigma (St. Louis, MO). Anti-A1 mAb CR1 was a generous gift from M ...
-
bioRxiv - Biochemistry 2023Quote: ... His tag and Flag tag (1:1000; F3165, Sigma).