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Citations for Millipore Sigma :
5401 - 5450 of 7654 citations for Parvovirus VP2 Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... The silver stained protein spots were de-stained using the ProteoSilver Plus Stain Kit (Sigma-Aldrich, MO, USA), and in-gel enzymatic digestion was done overnight at 37°C using Trypsin Singles ...
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bioRxiv - Physiology 2019Quote: ... Gels containing protein spots were then stained using the ProteoSilver Plus Silver Stain Kit (Sigma-Aldrich, MO, USA) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... and 0.1% NP-40 substitute (Fluka)) and added to 5-7 µL of protein A sepharose beads (Sigma) in a 50% slurry from the vendor that had been washed in IPP150 and conjugated to ∼5-10 µg of affinity-purified or protein A-purified anti-Prp43p ...
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bioRxiv - Neuroscience 2019Quote: ... Proteins were separated using SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (Immobilon-FL, EMD Millipore). Western blotting was performed using rabbit-anti-SRGAP2 N-terminal (raised against residues 193-205 ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified protein was buffer exchanged into TBS using a 10,000 Da molecular weight cut-off filter (Millipore-Sigma) through 3 successive washes ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified protein was buffer exchanged into TBS using a 10,000 Da molecular weight cut-off filter (Millipore-Sigma) through 3 successive washes ...
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bioRxiv - Molecular Biology 2020Quote: ... Incubated RdRp complex protein was concentrated with a 100 kDa molecular weight cut-off Centrifugal Filter (Millipore Corporation) and then size-separated by a Superdex S200 10/300 GL column in 25 mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA-cross-linked proteins were immunoprecipitated (1% kept as input) using 5 μg of NF-kB/p65 (Millipore) or control mouse IgG antibody ...
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bioRxiv - Cell Biology 2019Quote: ... Protein extracts were then loaded on 10% SDS-page acrylamide gels and transferred on PVDF membrane (Millipore #IPVH00010) overnight ...
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bioRxiv - Cell Biology 2020Quote: ... the proteins were eluted by adding 125 ng/ml FLAG peptide to the OG-containing buffer (Sigma-Aldrich) and incubating the beads for 30 min per round of elution ...
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bioRxiv - Bioengineering 2019Quote: ... Lyophilized proteins were purchased: human serum albumin (HSA; from human plasma, ≤0.02% Fatty acids, Lot #SLBZ2785, Millipore Sigma) and fibrinogen (FBG ...
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were then trypsinised by incubating with 5 µg/ml trypsin in 25 mM triethylammonium bicarbonate (Sigma-Aldrich) overnight ...
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bioRxiv - Developmental Biology 2019Quote: ... then resuspended in 2ml papain solution [0.75% v/v of papain (25mg/ml, 17U/mg protein, Sigma-Aldrich), 40μg/ml DNase I type IV ...
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bioRxiv - Microbiology 2019Quote: ... The eluted protein complex was concentrated using a 100 kD MWCO Amicon Ultra Centrifugal Filter Unit (EMD Millipore). The concentrated protein complex was further purified using size-exclusion chromatography with a Superose-6 Increase 10/300-GL (GE Life Sciences ...
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bioRxiv - Microbiology 2019Quote: ... The eluted LytH protein was concentrated using a 50 kD MWCO Amicon Ultra Centrifugal Filter Unit (EMD Millipore). The LytH protein was further purified using size-exclusion chromatography with a Superdex 200 Increase 10/300 GL column (GE Life Sciences ...
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bioRxiv - Neuroscience 2019Quote: ... 700-1000 µg total protein was incubated overnight at 4°C with 1:100 anti-FLAG (Sigma, F1804) or anti-Myc (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein samples were denatured and then separated on SDS-PAGE and transferred to PVDF membrane (Millipore, Burlington, USA). After blocking by non-fat milk for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... Fractions containing the protein were pooled and concentrated using an Amicon with a 3 KDa cut-off (Millipore). The protein was conserved in 10 mM Tris-HCl pH7.63 and 33% glycerol ...
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bioRxiv - Biochemistry 2020Quote: ... the proteins were concentrated using Amicon Ultra centrifugal filter devices (30 kDa molecular weight cut-off, EMD Millipore), and were quantified by Pierce™ BCA Protein Assay Kit (Thermo Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... at a concentration of 1 μg/ml or anti-ubiquitylated protein antibodies (clone FK2, Millipore Sigma #04-263) at a dilution of 1:400 for 3 hours at room temperature.
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A key interfacial residue identified with in-cell structure characterization of a class A GPCR dimerbioRxiv - Biochemistry 2019Quote: Protein samples were separated on a 10% SDS-PAGE and transferred to nitrocellulose membranes (Millipore Corporation, Billerica, USA). The non-denaturing condition was achieved with the use of SDS-free sample buffer and without sample boiling before loading sample ...
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bioRxiv - Plant Biology 2019Quote: ... StrepII-tagged proteins were detected using Strep-Tactin AP conjugate (IBA) or a mouse monoclonal StrepII antibody (Sigma). Further primary antibodies used were α-mCherry (Abcam ...
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bioRxiv - Biochemistry 2020Quote: ... Lamin proteins were detected using mouse anti-myc antibodies (clone 4A6, Millipore cat #05-724; 1:10000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR) ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant (total protein concentration ∼3 mg/ml) was incubated with ANTI-FLAG M2 agarose beads (Sigma Aldrich) at 4°C for 1 h with agitation ...
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bioRxiv - Genetics 2021Quote: ... Complexes were diluted to 2.5uM in a protein storage buffer composed of 50% (v/v) glycerol (Sigma G5516), 100 mM NaCl (Fisher Scientific S271-3) ...
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bioRxiv - Genomics 2020Quote: ... Approximately 10 mg of total protein was added to 30 μl anti-FLAG M2 magnetic beads (Sigma # M8823) prepared according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... anti-parvalbumin (PV;P3088) and anti-glial fibrillary acidic protein (GFAP;G3893) antibodies were obtained from Sigma-Aldrich; bromodeoxyuridine (BrdU ...
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bioRxiv - Molecular Biology 2021Quote: ... The elution of PAN-protein complexes was performed using 0.5 μl Benzonase nuclease (125U final, EDM Millipore 71206) in 1 ml of 0.35 M NaCl for 2 h at 37 °C (45 ...
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bioRxiv - Microbiology 2021Quote: ... MERS S-protein was detected using a monoclonal anti-FLAG M2-HRP conjugated antibody (SIGMA, A8592 @ 1:2000) which bound to a C-terminal FLAG-tag ...
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bioRxiv - Microbiology 2021Quote: ... Equal protein amount were used to perform SDS-PAGE and transferred onto polyvinylidene difluoride membranes (PVDF) (Millipore, USA) by the semi-dry transfer (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were extracted using RIPA buffer with protease inhibitors (cOmplete™, EDTA-free Protease Inhibitor Cocktail; Millipore Sigma). Sample input was normalized using a Bradford protein assay that was calibrated with BSA standards ...
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bioRxiv - Cell Biology 2021Quote: ... Expression of EGFP fusion proteins was then induced by addition of 1 µg/mL doxycycline (Sigma-Aldrich, D9891) and EGFP expressing cells were positively selected by FACS ...
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bioRxiv - Biochemistry 2021Quote: 100 bacterial homologs of nucleobase/cation symporter 2 (NCS2) proteins were cloned into a modified pET28 plasmid (Novagen) with either an N- or a C-terminal deca-histidine tag and a TEV protease recognition site at the central facility of the New York Consortium on Membrane Protein Structure (NYCOMPS ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was treated by 0.5 mg/mL drICE protease and incubated with 2 mM NADPH (Sigma-Aldrich) for 2 h at 4 °C ...
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bioRxiv - Bioengineering 2021Quote: ... Fractionation of the nuclear protein was performed according to the Pierce cytoplasmic and nuclear extraction kit instructions (Sigma). Samples were run on an SDS-Page gel (BioRad ...
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bioRxiv - Bioengineering 2020Quote: ... Protein was covalently bound to the slide surface via addition of freshly prepared 2.5 mg/mL NaBH4 (Sigma) dissolved in 25 %v/v ethanol in PBS at RT for 5 min ...
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bioRxiv - Biochemistry 2021Quote: ... the protein was purified from the cleared lysate by affinity to Anti-FLAG M2 Affinity gel (Sigma-Aldrich) and eluted in Flag buffer containing 0.5 mg/ml 3xFLAG peptide ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was purified from the cleared lysate by affinity to Anti-FLAG M2 Affinity gel (Sigma-Aldrich) and eluted with pulldown buffer containing 0.1 mg/ml 3xFlag peptide ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were concentrated using 3 kDa (KrACP, FabD) and 30 kDa (KrKSCLF) Amicon Ultra (Millipore, 15 mL capacity) molecular weight cutoff (MWCO ...
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bioRxiv - Immunology 2020Quote: For immunoprecipitation experiments the Chk1 or GATA3 antibody was incubated with Red Protein G Affinity beads (Sigma-Aldrich) for 1 hour at 4°C on a shaker to allow the antibody to bind to the beads ...
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bioRxiv - Biophysics 2021Quote: ... Next, dissolved membrane proteins (1 nmol) and MSP (50 μL, 1 mg/mL) (MSP1E3D1, Sigma-Aldrich, No. M7074) were added to the lipid suspension and mixed for ∼1 h while rotating in the dark at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was recovered and the protein concentration was estimated using the Direct Detect® Infrared Spectrophotometer (Millipore). 20 μg proteins were loaded on 4-15% Mini-PROTEAN® TGX Precast Protein Gels Stain Free™ (Bio-Rad® ...
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bioRxiv - Microbiology 2021Quote: ... For elution of FLAG-tagged proteins and their complexes 100 µL of 0.2 mg/mL FLAG3 peptide (Sigma) was added to samples ...
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bioRxiv - Cancer Biology 2021Quote: ... Per sample equal amounts of protein were separated by Bis-Tris-PAGE and transferred to PVDF membranes (Millipore). The membranes were incubated with 5% (w/v ...
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bioRxiv - Cancer Biology 2020Quote: ... The pETDuet-1-His-BAP1-ULD+ASXL2-AB protein complex was expressed in Rosetta 2 (DE3) pLysS (Millipore). The bacteria bearing the desired plasmids were propagated with aeration at 37°C in 1L of 2YT to an A600 absorbance of approximately 0.6 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by SDS-PAGE and transferred onto Immobilon-P® PVDF transfer membranes (IPVH00010; EMD Millipore) as previously described (Haynes et al. ...
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bioRxiv - Pathology 2021Quote: ... 2.5-20μg of total protein extracts were separated on acrylamide-SDS gels and transferred to PVDF membranes (Millipore). Specific proteins were detected by using different antibodies listed in supplementary table 1 ...
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bioRxiv - Physiology 2020Quote: ... and purified cell surface proteins were analyzed by western blot using the mαNaVPAN antibody (1:2000, Sigma, #S8809), the anti-transferrin receptor mouse monoclonal antibody (TransR ...
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bioRxiv - Plant Biology 2021Quote: ... Protein complexes were eluted with 100 μl IP buffer supplemented with 200 μg/ml 3xFLAG peptide (Merck Millipore). The eluted protein was then immobilized on anti-c-Myc magnetic beads (Thermo Fisher ...
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bioRxiv - Biochemistry 2020Quote: ... and the sample supernatant was filtered using low-protein binding bottle-top filters (Millipore Express Plus 0.22 µm). This filtered supernatant was supplemented with final concentrations of 2 mM EDTA (stock EDTA 200 mM ...