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Citations for Millipore Sigma :
5351 - 5400 of 7654 citations for Parvovirus VP2 Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: The in vitro phosphorylation is conducted using the human Src-GST protein (Sigma-Aldrich, Catalogue number: S1076) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... The DNA was digested by incubation with benzonase (Sigma, 25 U per expected 1 mg of protein) for 30 min at room temperature ...
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bioRxiv - Biochemistry 2024Quote: The NSP3(1-110) protein was then immobilized on Anti-Flag M2 Affinity gel (Sigma Aldrich, A2220), and the cell lysates from stable cell line 293T-N ...
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bioRxiv - Biochemistry 2024Quote: ... Bound protein was eluted off agarose gel with 30-50 μL 1X 3XFLAG peptide (Sigma catalog #F4799) at 4 °C with rotation for 1 h ...
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bioRxiv - Biophysics 2024Quote: ... Then 495 µL of the protein sample was mixed with 55 µL 99.8% deuterated water (Sigma-Aldrich). The diffusion NMR NMR samples for the PHL4FL and PHL4effector domain were prepared by concentrating pure non-isotopically labeled ...
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bioRxiv - Cell Biology 2024Quote: ... The eluted proteins were collected and concentrated to 500 µL with a 30K MWCO concentrator (EMD Millipore). Concentrated Ndel1 was then purified via SEC on a Superdex200 10/300 column (Cytiva ...
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bioRxiv - Biophysics 2024Quote: ... cells were resuspended and lysed in BugBuster® Master Mix Protein Extraction Reagent (Millipore Sigma, Cat# 71456) at room temperature following the manufacturers recommendation ...
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bioRxiv - Cell Biology 2024Quote: ... Twenty micrograms of total protein were separated on 10% SDS-PAGE and transferred onto PVDF membranes (Millipore). The resulting membranes were blocked for 1 hour at 37°C with TBST buffer (12 mM Tris-HCl ...
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bioRxiv - Microbiology 2024Quote: For top-down EfpL measurements the proteins were desalted on the ZipTip with C4 resin (Millipore, ZTC04S096) and eluted with 50 % (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of proteins were separated by SDS-PAGE and transferred to PVDF membranes (Millipore, Cat. IPVH00010). Membranes were incubated with primary antibodies in 5% BSA in 1x TBST buffer overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were then transferred to nitrocellulose membrane for immunodetection using mouse anti-puromycin (1:1000,12D10, MABE943 Sigma) and mouse anti-actin (1:25 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein fractions from a single peak were collected and concentrated in 50 kDa MWCO concentrator (Millipore Sigma), flash frozen in liquid nitrogen and stored at −80 °C until further use ...
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bioRxiv - Biochemistry 2024Quote: ... Protein fractions from a single peak were collected and concentrated in 50 kDa MWCO concentrator (Millipore Sigma), flash-frozen in liquid nitrogen and stored at −80 °C until further use ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 µM protein and 5 mg/ml of one of the following carbohydrates was used: maltose (Sigma), maltotriose (Carboexpert-BoGH13BSus/SusA ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were separated using SDS-PAGE and subsequently transferred to a polyvinylidene fluoride membrane (PVDF, Millipore Corporation). Then blocked PVDF membrane with blocking buffer (5% fat-free milk ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 mL of the cleared lysate (∼2.5 mg total protein) was combined with anti-Flag (Sigma, 1804) coated Dynabeads Protein G (Invitrogen 10003D ...
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bioRxiv - Molecular Biology 2024Quote: ... Equivalent amounts of sample proteins in Laemmli buffer (With reducing agent, 2-Mercaptoethanol (Sigma-Aldrich, CAT# 8057400005) were electrophoresed on 4-20% Mini-PROTEAN® TGX Stain-Free gels (Bio-rad ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin concentrations were normalized among samples and pre-cleared with Protein G agarose beads (Millipore, 100 μL). The chromatin samples were split in two and diluted with IP buffer (10 mM Tris-HCl pH = 8.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 µg of protein was reduced with 5 mM Tris(2-carboxyethyl) phosphine hydrochloride (TCEP) (Sigma Aldrich) for 2 min and alkylated with 20 mM chloroacetamide (CAA ...
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bioRxiv - Molecular Biology 2024Quote: ... diluted and precleared with 30 μL protein A/G agarose beads (Merck-Millipore, 16-157/16-201) for 1h at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was cleaved from the GST tag by incubating the beads with human thrombin (Sigma-Aldrich) in the presence of 50 µM CaCl2 overnight at 4 °C with rocking ...
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bioRxiv - Plant Biology 2024Quote: ... Purified proteins were further concentrated using Amicon Ultra centrifugal filter device (3 kDa MW cut-off, Millipore). Protein quantity was measured using Bradford method (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... The eluted protein solution was in turn transferred to an ultrafiltration column (Amicon Ultra-15, Merck Millipore) and concentrated by centrifugation ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were separated by SDS-PAGE and transferred onto 0.45 µm pore size Immobilon PVDF membrane (Millipore). PVDF membranes were blocked with TBS-T (50 mM Tris ...
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bioRxiv - Synthetic Biology 2024Quote: ... Purified protein was concentrated using an Amicon Ultra-4 Centrifugal 50 kDa Filter Units (Sigma Aldrich NV). Protein was quantified against a standard curve made with Bovine Serum Albumin (BSA) ...
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bioRxiv - Developmental Biology 2024Quote: ... that had been pre-coated with Protein A from Staphylococcus aureus (Sigma P6031; 100 ng/μl PA) and contained 800 μl culture media (Leibovitz’s L15 Medium ...
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bioRxiv - Molecular Biology 2024Quote: ... and in N2B27 serum-free media containing Mitogen-Activated Protein Kinase (MEK) inhibitor PD0325901 (1 μM) (Millipore), Glycogen Synthase Kinase 3 (GSK3 ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μg of purified His fusion protein (PuBZR1-His) was bound to Ni-NTA His binding resin (Novagen). GST fusion proteins containing PuACO1 (PuACO1-GST ...
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bioRxiv - Cancer Biology 2021Quote: Proteins were extracted from cells in either radioimmunoprecipitation assay (RIPA) buffer 1 mM protease inhibitor cocktail (Sigma-Aldrich). Proteins were loaded on PVDF membranes ...
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bioRxiv - Cancer Biology 2021Quote: Proteins were extracted from cells in either radioimmunoprecipitation assay (RIPA) buffer 1 mM protease inhibitor cocktail (Sigma-Aldrich). Samples were heated at 100 °C for 10 min in a 4X loading buffer ...
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bioRxiv - Microbiology 2020Quote: ... The digested proteins were visualized in a 10% acrylamide gel stained with Coomassie Brilliant Blue R (Sigma Aldrich)
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bioRxiv - Cell Biology 2020Quote: ... Proteins were visualized with 1:20,000 anti-rabbit IgG (whole molecule)-peroxidase secondary antibody (Sigma; A0545, lot 022M4811) and SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred from gels to PVDF membrane (Immobilon-FL, pore size 0.45 μm, Millipore, catalogue number IPFL00010) for immunoblotting ...
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bioRxiv - Cell Biology 2020Quote: ... a fraction of Proteins was separated on 12% SDS-PAGE gels and transferred to Immobilon PVDF membranes (Millipore). The membranes were blocked in 5% milk or 5% BSA (depending on the primary antibody) ...
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bioRxiv - Cell Biology 2020Quote: ... The lysates were cleared from insoluble chromatin and were subjected to immunoprecipitation with protein A agarose beads (Millipore) for 1.5 hrs at 4°C ...
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bioRxiv - Immunology 2021Quote: ... The protein was concentrated using a 30 kDa MWCO Amicon ultra-15 centrifugal filter (Sigma, cat. no. UFC903024), and stored at −70°C ...
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bioRxiv - Immunology 2021Quote: ... The eluted protein was incubated with 0.5 mM ADP and 0.5 mM MCC950 (Millipore Sigma, Cat. no: 538120), 0.5 mM dATP or no additives for 30 min on ice ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 ul protein A/G beads pre-saturated with 5 mg/ml BSA in PBS (Sigma, A-3311) were washed in RIPA buffer and subsequently added to the chromatin/antibody mix at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... and subsequently blocked with 0.5 % non-fat dried milk (AppliChem) in TBST using the Snap i.d.® 2.0 Protein Detection System (Millipore). The blot was then incubated for 10 min at room temperature with a 1:1000 dilution of rabbit polyclonal anti-ASIC1a antibody (OSR00097W ...
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bioRxiv - Neuroscience 2021Quote: ... protein extracts from SCN cultures were examined by SDS-PAGE immunoblotted with anti-UBE3A monoclonal antibody (E8655, Sigma), or anti-FLAG monoclonal antibody (F1804 ...
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bioRxiv - Molecular Biology 2020Quote: ... Immunoprecipitation of FLAG-AGO2 was performed with Dynabeads Protein G (Life) and anti-FLAG M2 mouse (Sigma, F3165) for 3h at 4ºC with rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentrations for the whole cell lysates and the unbound fractions were determined using Bradford reagent (Sigma-Aldrich). For immunoprecipitation ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-GAPDH (1:2000, Protein Tech, #10494-1-AP) and anti-rabbit IgG-AP (1:20000, Sigma, #A3687)
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bioRxiv - Cell Biology 2022Quote: ... Bound proteins were eluted with 5 packed bead volumes of 150 ng/μL 3xFLAG peptide (Sigma-Aldrich, F4799) in TBS for 30 min at 4 °C with rotation.
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: ... Cells were then incubated for 15 minutes in 2% BSA with globular actin-binding protein GC globulin (Sigma). Following incubation ...
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bioRxiv - Immunology 2022Quote: ... Single colonies were inoculated and protein expression was induced using isopropyl-β-D-thiogalacto-pyranoside (IPTG, Sigma Aldrich). Cell lysates were screened for protein expression using SDS-PAGE followed by Coomassie blue staining ...
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bioRxiv - Microbiology 2020Quote: ... ii) 300 μg C g−1 13C-labelled Algal crude protein extract-98 atom% 13C (ISOTEC, Sigma-Aldrich), iii ...
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bioRxiv - Immunology 2021Quote: ... Proteins were electrophoretically separated and transferred to polyvinylidene fluoride (PVDF) membranes (Immobilon P, Merck Millipore, Burlington, MA, USA). Blocking treatment was performed using the PVDF Blocking Reagent for Can Get Signal (Toyobo ...
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bioRxiv - Molecular Biology 2021Quote: ... StrepII-tagged proteins (RBBP6, symplekin and WDR33) were detected using streptavidin-HRP conjugate (Merck Millipore, cat. No. 18152) and Amershan ECL Detection Reagents (Cytiva ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein levels of wild-type or mutant Rad6 were determined by immunoblotting using anti-Flag M2 antibody (Sigma).