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Citations for Millipore Sigma :
5351 - 5400 of 10000+ citations for Suppressor of CDC2 With RNA Binding Motif 2 RBMS1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... RNA was extracted from the in vitro samples using the Spectrum™ Plant Total RNA kit (Sigma-Aldrich, MO, USA) according to the manufacturers’ instructions ...
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bioRxiv - Plant Biology 2021Quote: ... RNA was isolated at different time points using Spectrum™ Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA). Aliquots with 50 ng RNA were subjected to DNAse treatment (Thermo Scientific) ...
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bioRxiv - Cell Biology 2021Quote: RNA was purified from lysates (total cellular RNA) and separately from SG or mFA core fractions using TRI-reagent (Sigma). Total RNA was approximately 10 times more concentrated than RNA from SG/mFA core fractions (∼100 ng/µl vs ...
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bioRxiv - Plant Biology 2022Quote: ... total RNA was extracted from 14 day-old Arabidopsis thaliana seedlings using the SpectrumTM Plant Total RNA Kit (Sigma-Aldrich) and treated with DNAase I (RapidOut DNA Removal Kit ...
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bioRxiv - Pathology 2022Quote: ... resuspended in RNA extract lysis buffer (100µL RLT lysis buffer from RNA extraction kit supplemented with 40mM DTT, Sigma, #D9779), vortexed and let sit (10min. ...
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bioRxiv - Plant Biology 2021Quote: ... Total RNA was extracted from 100 mg of whole seedling tissue using the Spectrum Plant Total RNA Kit (Sigma, STRN50) and l μl of eluted RNA was used for complementary DNA (cDNA ...
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bioRxiv - Immunology 2020Quote: Total RNA was extracted from lung tissue or sorted cells as indicated in the text with Total RNA purification kit (Sigma) and treated with DNase I (Invitrogen) ...
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bioRxiv - Developmental Biology 2019Quote: ... Digoxigenin (DIG)-labeled RNA probes were prepared by in vitro transcription with linearized constructs using DIG RNA Labeling Kit (SP6/T7) (Sigma). Embryos were fixed in 4% PFA in PBS ...
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bioRxiv - Microbiology 2021Quote: ... A small fraction of m7G*ppp-RNA and of G*ppp-RNA were digested with 5 μg nuclease P1 (Sigma) in 50 mM NaOAc pH5.2 buffer to originate the m7G*ppp and G*pppG markers in the thin-layer chromatography (TLC ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was extracted from leaf tissue using the Spectrum Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA) following Protocol A.
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bioRxiv - Biochemistry 2020Quote: To examine the RNA binding mode of the N-NTD we used a commercially available 7mer RNA duplex that was prepared by annealing of RNA oligonucleotides 5’-CACUGAC-3’ and 5’-GUCAGUG-3’ (Sigma). The RNA oligonucleotides were mixed in a molar ratio 1:1 at the final concetration 200 μM of each oligonucleotide and water supplemented with 50 mM NaCl ...
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bioRxiv - Genomics 2019Quote: ... Total RNA was isolated from control and stressed plants root samples using the Sigma plant RNA kit (Sigma, Inc, USA). RNA quality and concentration were determined by Qubit 2.0 fluorometer (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: RNA extraction using Trizol-Total RNA was extracted using the TRI reagent kit according to the manufacturer’s instructions (Sigma-Aldrich).
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bioRxiv - Plant Biology 2023Quote: ... total RNA was extracted from each tissue using the Spectrum Plant Total RNA kit (Sigma-Aldrich, St. Louis, MO, USA) with 2% PVP40 added to the extraction buffer to sequester phenolic inhibitors ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA from sucrose gradient fractions with heparin (55 u/ml) were extracted with the RNA Purification kit (Sigma RNB100-50RXN). Northern blots were performed as described elsewhere45 ...
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bioRxiv - Plant Biology 2024Quote: ... were detached and immediately snap frozen in liquid N2 and ground in a tissue lyser for RNA isolation using TRI RNA Isolation Reagent (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: Seedlings were harvested in liquid nitrogen and total RNA was extracted using a Spectrum Plant Total RNA kit (Sigma-Aldrich). The sequencing was made on an Illumina HiSeq 2500 system using 100-bp single-end reads protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Seedlings were flash-frozen in liquid nitrogen before total RNA was extracted using SpectrumTM Plant Total RNA Kit (SIGMA Aldrich).
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bioRxiv - Plant Biology 2023Quote: ... and RNA was extracted from three to four replicates per plant and stage using the Spectrum Plant Total RNA kit (SIGMA). MagNA Lyser Green Beads (Roche ...
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bioRxiv - Genomics 2023Quote: ... The material was ground with mortar and pestle and the RNA was extracted with the Spectrum Plant Total RNA kit (Sigma) and treated with DNAse I amplification grade (Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: ... the PCR products were used as templates for in vitro transcription of DIG-labeled RNA probe using T7 RNA polymerase (SIGMA) and DIG RNA Labeling Mix (SIGMA ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was extracted using GenEluteTM Mammalian Total RNA Miniprep Kit and On-Column DNase I Digestion Set (both Sigma-Aldrich) and a total of 9 samples fulfilling the submission requirements (0.5-1.0μg total RNA in nuclease free dH2O with A260/A280 and A60/A230 ratios above 1.8 ...
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bioRxiv - Plant Biology 2024Quote: All samples were homogenised using Geno/Grinder 2010 (SPEX SamplePrep) and total RNA was isolated from homogenised samples using the Sigma Spectrum Plant Total RNA kit (Sigma) supplemented with the On-Column DNase I Digestion step to remove genomic DNA ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA from control and treated samples was extracted after Sirodesmin PL treatment using a Plant Spectrum Total RNA Kit (Sigma), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: Proteins of interest were labeled with well-validated custom and commercial antibodies: Connexin43 (Cx43; mouse monoclonal antibody, Cat. #: MAB3067, EMD Millipore Corp., Darmstadt, Germany; rabbit polyclonal antibody, Cat. # C6219, Millipore Sigma, St. Louis, MO), N-cadherin (N-cad ...
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bioRxiv - Biophysics 2019Quote: ... Cultures were then diluted in synthetic complete low-fluorescence media (SC-LFM) and either 2% galactose (Acros, cat. 150615000) or 2% (w/v) dextrose (Sigma, cat. G5767). After three more hours of incubation ...
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bioRxiv - Immunology 2020Quote: ... isolated brains were post-fixed in 2% PFA overnight and embedded in 2% low-melt agarose (Sigma-Aldrich, St. Louis, MO, USA) in DPBS ...
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bioRxiv - Molecular Biology 2019Quote: ... dissolved in 100 mL PBS containing Ca2+/Mg2+ and 25 mM N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) (HEPES; Sigma, Vienna, Austria), resulting in a final collagenase concentration of 0.1 U/mL ...
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bioRxiv - Bioengineering 2021Quote: ... The yeasts were grown in yeast extract (1%, w/v) peptone (2%, w/v) glucose (2%, w/v) (YPD) media supplemented with 50mg/L ampicillin (Sigma-Aldrich, 69534). The strains were grown in 250 mL Erlenmeyer flasks containing 100 mL of YPD ...
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bioRxiv - Neuroscience 2020Quote: ... Each sample was diluted 1/10 with McIlvaine citrate-phosphate buffer pH 4.5 and activity assayed with 4-methylumbelliferyl-2-acetamido-2-deoxy-β-D-gluco-pyranoside (Sigma, Dorset, UK) in a plate reader.
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bioRxiv - Biochemistry 2022Quote: ... pLANT-2/RIL–RFC[1+5] was co-transformed with pET(11a)-RFC[2+3+4] into BLR(DE3) cells (Novagen, Madison, Wisconsin). The cell lysate was clarified by centrifugation ...
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bioRxiv - Neuroscience 2020Quote: PF-4778574 [N-<(3R,4S)-3-[4-(5-cyano-2-thienyl)phenyl]tetrahydro-2H-pyran-4-yl>propane-2-sulfonamide] was obtained as a powder from Sigma (catalog #PZ0211) and solubilized (2.5 mg/ml ...
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bioRxiv - Microbiology 2021Quote: ... Three subsamples (∼2 ml) were taken from each section with a 3 ml syringe and immediately fixed in 2 ml RNAlater (Sigma Life Science) in a 5 ml tube (Qiagen ...
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bioRxiv - Immunology 2022Quote: Fabs (2-4 mg/ml) were mixed with a 2 to 3-fold molar excess of fen-G4 or fentanyl (Sigma F-013) in Dulbecco’s PBS and incubated for 1-2 hours at 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... The following chemicals were prepared and used according to the manufacturers’ guidelines: 2-deoxy-D-glucose (2-DG, Sigma, cat. no. D8375), Na-iodoacetate (Sigma ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... SB was suspended in 10% 2-hydroxypropyl-b-cyclodextrin in sterile water and 2% dimethyl sulfoxide (Sigma-Aldrich, St. Louis, MO, USA). Three different group of rats were treated with SB as follows ...
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bioRxiv - Molecular Biology 2020Quote: ... sections of mouse heart were perfused for 1-2 min with 2ml of 2% TTC (Sigma-Aldrich Co., St. Louis, MO, USA) in phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2020Quote: A stock solution (74.8 mM) of the MEK inhibitor 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one (PD98059, PD hereafter, Sigma-Aldrich, Argentina) was conserved at −20 °C and freshly diluted in DMSO to the desired concentration (11.22 mM ...
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bioRxiv - Biophysics 2021Quote: ... the solutions were replaced by the fixing solution overnight (2% glutaraldehyde, 2% paraformaldehyde [EM grade; Science Service GmbH, Germany] in 100 mM aqueous sodium cacodylate buffer [Sigma Aldrich, Germany]). Without rinsing the cells ...
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bioRxiv - Physiology 2022Quote: ... the mice were deeply anesthetized with isoflurane and the brains were sectioned into 2-mm-thick sections and placed in 2% 2,3,5 triphenyltetrazolium chloride (TTC, cat. #T8877, Sigma-Aldrich, St. Louis, MO) for 10 min at 37 °C to delineate infarcts ...
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bioRxiv - Plant Biology 2024Quote: ... specific primers for each T-DNA line (Supplemental Table 2) were designed using SALK site (http://signal.salk.edu/tdnaprimers.2.html) and were ordered from Sigma (https://www.sigmaaldrich.com/GB/en).Ten seeds from each T-DNA mutant line were sown and seedlings were picked and transferred to pots ...
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bioRxiv - Cancer Biology 2024Quote: ... medium supplemented with 10 mM of 2-(4-(2-hydroxyethyl) piperazin-1-yl) ethane sulfonic acid (HEPES; Sigma-Aldrich, St. Louis, MO), 24 mM sodium bicarbonate ...
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bioRxiv - Microbiology 2024Quote: ... the maintenance and expansion of T cells was done by adding medium with 10 ng/ml interleukin-2 (IL-2, Stemcell) (10 ng/ml) and for activation Lectin from Phaseolus vulgaris (red kidney bean, PHA) (Sigma, 1µg/ml).
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bioRxiv - Plant Biology 2023Quote: ... Yeast cells were grown in rich medium (YPD; 1% yeast extract [Difco, 10215203], 2% peptone [Difco, 211705], and 2% glucose [wt/vol]) (Sigma-Aldrich, G7021) on an orbital shaker (200 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... sonicated for 2 minutes of 2 sec on/8 seconds off cycles on ice using probe sonicator (Ultrasonic processor 500W, Sigma Aldrich, USA). 3-5 mL of 5 % NaCl was added to the samples and after thorough vortexing the phases were separated by centrifugation at 3,000 × g for 5 min ...
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bioRxiv - Cancer Biology 2023Quote: Tumors excised from AKB6 xenografts that were treated with vehicle (n=2) and MRTX1133 (n=2) were digested using Liberase (Sigma Cat # 05401020001) to get single cells and sent to RPCI’s Genomics Shared Resources for sequencing using 10X Chromium and Illumina’s NovaSeq instruments ...
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bioRxiv - Biochemistry 2022Quote: ... were mixed with a stock solution of 40% positively charged (3-acrylamidopropyl)-trimethylammonium chloride (75% wt, Aldrich) or negatively charged 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS, Sigma-Aldrich, Inc) containing N,N’-methylenebisacrylamide (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... were resuspended 2 mL of viral particle supernatant for E:T of 2:1 with 8 μg/mL of Polybrene (Millipore Sigma, Burlington, MA), maintained in at 37°C for 4 hours ...
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bioRxiv - Microbiology 2023Quote: ... Cell-laden filters were suspended in 2-ml bead beater tubes (SSIbio, # 21276) containing 1 ml of fresh metabolite extraction solvent (2:2:1 ratio of acetonitrile (Sigma-Aldrich, # 900667), methanol (>99.8%) ...
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bioRxiv - Neuroscience 2024Quote: ... the samples were incubated with fluorescent dye-conjugated secondary antibodies at RT for 2 hours with 1% NDS in PBST with 4’,6-diamidino-2-phenylindole (DAPI, 1:1000, Sigma-Aldrich, D9542). Afterward ...