Labshake search
Citations for Millipore Sigma :
5251 - 5300 of 7633 citations for Yellow Fever Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... Protein bands were visualized on X-ray film by electro-chemiluminescence (Immobilon Western HRP Substrate, WBKLS0500, Millipore). For the isolation of chromatin-enriched proteins ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The pellets were resuspended in 50 μL of BugBuster Protein Extraction Reagent (Millipore Sigma, SKU 70584-4) and vortexed on Benchmark Scientific Incu-Mixer MP at room temperature ...
-
bioRxiv - Neuroscience 2023Quote: ... The protein sample was concentrated using 100kDa MWCO Amicon® Ultra-15 Centrifugal Filter Unit (EMD Millipore) to a final concentration of ∼0.8mg/ml and filtered by 0.22 µm pore size PVDF membrane prior to deposition on the grid for EM imaging.
-
Aromatase in adipose tissue exerts an osteoprotective function in male mice via phosphate regulationbioRxiv - Physiology 2024Quote: ... The separated proteins were transferred by electrophoresis to Immobilon-P polyvinylidene difluoride membrane (Millipore, Billerica, MA, USA) and treated with diluted antibodies ...
-
bioRxiv - Biophysics 2024Quote: NMR experiments were performed on protein in 90% NMR Buffer and 10% deuterated water (99.8%, Sigma-Aldrich). The PHL4effector domain concentration was 73.2 µM for the 13C and 15N labeled sample and 98.2 µM for the 15N labeled sample ...
-
bioRxiv - Biophysics 2024Quote: ... The cleaved WW4-HECT protein was concentrated (Amicon Ultra-15 centrifugal filter, 10 kDa cutoff; EMD Millipore), filtered ...
-
bioRxiv - Cancer Biology 2024Quote: ... Bound material was recovered after incubation with 15 μL of protein G beads (Millipore, cat # 16-266) at 4°C ...
-
bioRxiv - Biochemistry 2024Quote: The in vitro phosphorylation is conducted using the human Src-GST protein (Sigma-Aldrich, Catalogue number: S1076) according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2024Quote: ... The DNA was digested by incubation with benzonase (Sigma, 25 U per expected 1 mg of protein) for 30 min at room temperature ...
-
bioRxiv - Biochemistry 2024Quote: The NSP3(1-110) protein was then immobilized on Anti-Flag M2 Affinity gel (Sigma Aldrich, A2220), and the cell lysates from stable cell line 293T-N ...
-
bioRxiv - Biochemistry 2024Quote: ... Bound protein was eluted off agarose gel with 30-50 μL 1X 3XFLAG peptide (Sigma catalog #F4799) at 4 °C with rotation for 1 h ...
-
bioRxiv - Biophysics 2024Quote: ... Then 495 µL of the protein sample was mixed with 55 µL 99.8% deuterated water (Sigma-Aldrich). The diffusion NMR NMR samples for the PHL4FL and PHL4effector domain were prepared by concentrating pure non-isotopically labeled ...
-
bioRxiv - Cell Biology 2024Quote: ... The eluted proteins were collected and concentrated to 500 µL with a 30K MWCO concentrator (EMD Millipore). Concentrated Ndel1 was then purified via SEC on a Superdex200 10/300 column (Cytiva ...
-
bioRxiv - Biophysics 2024Quote: ... cells were resuspended and lysed in BugBuster® Master Mix Protein Extraction Reagent (Millipore Sigma, Cat# 71456) at room temperature following the manufacturers recommendation ...
-
bioRxiv - Cell Biology 2024Quote: ... Twenty micrograms of total protein were separated on 10% SDS-PAGE and transferred onto PVDF membranes (Millipore). The resulting membranes were blocked for 1 hour at 37°C with TBST buffer (12 mM Tris-HCl ...
-
bioRxiv - Microbiology 2024Quote: For top-down EfpL measurements the proteins were desalted on the ZipTip with C4 resin (Millipore, ZTC04S096) and eluted with 50 % (v/v ...
-
bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of proteins were separated by SDS-PAGE and transferred to PVDF membranes (Millipore, Cat. IPVH00010). Membranes were incubated with primary antibodies in 5% BSA in 1x TBST buffer overnight at 4°C ...
-
bioRxiv - Microbiology 2024Quote: ... Proteins were then transferred to nitrocellulose membrane for immunodetection using mouse anti-puromycin (1:1000,12D10, MABE943 Sigma) and mouse anti-actin (1:25 ...
-
bioRxiv - Biochemistry 2024Quote: ... Protein fractions from a single peak were collected and concentrated in 50 kDa MWCO concentrator (Millipore Sigma), flash frozen in liquid nitrogen and stored at −80 °C until further use ...
-
bioRxiv - Biochemistry 2024Quote: ... Protein fractions from a single peak were collected and concentrated in 50 kDa MWCO concentrator (Millipore Sigma), flash-frozen in liquid nitrogen and stored at −80 °C until further use ...
-
bioRxiv - Microbiology 2024Quote: ... 0.5 µM protein and 5 mg/ml of one of the following carbohydrates was used: maltose (Sigma), maltotriose (Carboexpert-BoGH13BSus/SusA ...
-
bioRxiv - Cell Biology 2024Quote: ... proteins were separated using SDS-PAGE and subsequently transferred to a polyvinylidene fluoride membrane (PVDF, Millipore Corporation). Then blocked PVDF membrane with blocking buffer (5% fat-free milk ...
-
bioRxiv - Molecular Biology 2024Quote: ... 0.5 mL of the cleared lysate (∼2.5 mg total protein) was combined with anti-Flag (Sigma, 1804) coated Dynabeads Protein G (Invitrogen 10003D ...
-
bioRxiv - Molecular Biology 2024Quote: ... Equivalent amounts of sample proteins in Laemmli buffer (With reducing agent, 2-Mercaptoethanol (Sigma-Aldrich, CAT# 8057400005) were electrophoresed on 4-20% Mini-PROTEAN® TGX Stain-Free gels (Bio-rad ...
-
bioRxiv - Molecular Biology 2024Quote: ... Chromatin concentrations were normalized among samples and pre-cleared with Protein G agarose beads (Millipore, 100 μL). The chromatin samples were split in two and diluted with IP buffer (10 mM Tris-HCl pH = 8.0 ...
-
bioRxiv - Molecular Biology 2024Quote: ... 200 µg of protein was reduced with 5 mM Tris(2-carboxyethyl) phosphine hydrochloride (TCEP) (Sigma Aldrich) for 2 min and alkylated with 20 mM chloroacetamide (CAA ...
-
bioRxiv - Molecular Biology 2024Quote: ... diluted and precleared with 30 μL protein A/G agarose beads (Merck-Millipore, 16-157/16-201) for 1h at 4°C ...
-
bioRxiv - Biochemistry 2024Quote: ... The protein was cleaved from the GST tag by incubating the beads with human thrombin (Sigma-Aldrich) in the presence of 50 µM CaCl2 overnight at 4 °C with rocking ...
-
bioRxiv - Plant Biology 2024Quote: ... Purified proteins were further concentrated using Amicon Ultra centrifugal filter device (3 kDa MW cut-off, Millipore). Protein quantity was measured using Bradford method (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2024Quote: ... The eluted protein solution was in turn transferred to an ultrafiltration column (Amicon Ultra-15, Merck Millipore) and concentrated by centrifugation ...
-
bioRxiv - Cell Biology 2024Quote: ... The expression of the recombinant proteins was checked by Western blotting with monoclonal anti-HA antibodies (Sigma).
-
bioRxiv - Molecular Biology 2024Quote: ... Proteins were separated by SDS-PAGE and transferred onto 0.45 µm pore size Immobilon PVDF membrane (Millipore). PVDF membranes were blocked with TBS-T (50 mM Tris ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Purified protein was concentrated using an Amicon Ultra-4 Centrifugal 50 kDa Filter Units (Sigma Aldrich NV). Protein was quantified against a standard curve made with Bovine Serum Albumin (BSA) ...
-
bioRxiv - Developmental Biology 2024Quote: ... that had been pre-coated with Protein A from Staphylococcus aureus (Sigma P6031; 100 ng/μl PA) and contained 800 μl culture media (Leibovitz’s L15 Medium ...
-
bioRxiv - Molecular Biology 2024Quote: ... and in N2B27 serum-free media containing Mitogen-Activated Protein Kinase (MEK) inhibitor PD0325901 (1 μM) (Millipore), Glycogen Synthase Kinase 3 (GSK3 ...
-
bioRxiv - Plant Biology 2020Quote: ... 5 μg of purified His fusion protein (PuBZR1-His) was bound to Ni-NTA His binding resin (Novagen). GST fusion proteins containing PuACO1 (PuACO1-GST ...
-
bioRxiv - Cancer Biology 2021Quote: Proteins were extracted from cells in either radioimmunoprecipitation assay (RIPA) buffer 1 mM protease inhibitor cocktail (Sigma-Aldrich). Proteins were loaded on PVDF membranes ...
-
bioRxiv - Cancer Biology 2021Quote: Proteins were extracted from cells in either radioimmunoprecipitation assay (RIPA) buffer 1 mM protease inhibitor cocktail (Sigma-Aldrich). Samples were heated at 100 °C for 10 min in a 4X loading buffer ...
-
bioRxiv - Microbiology 2020Quote: ... The digested proteins were visualized in a 10% acrylamide gel stained with Coomassie Brilliant Blue R (Sigma Aldrich)
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were visualized with 1:20,000 anti-rabbit IgG (whole molecule)-peroxidase secondary antibody (Sigma; A0545, lot 022M4811) and SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred from gels to PVDF membrane (Immobilon-FL, pore size 0.45 μm, Millipore, catalogue number IPFL00010) for immunoblotting ...
-
bioRxiv - Cell Biology 2020Quote: ... a fraction of Proteins was separated on 12% SDS-PAGE gels and transferred to Immobilon PVDF membranes (Millipore). The membranes were blocked in 5% milk or 5% BSA (depending on the primary antibody) ...
-
bioRxiv - Cell Biology 2020Quote: ... The lysates were cleared from insoluble chromatin and were subjected to immunoprecipitation with protein A agarose beads (Millipore) for 1.5 hrs at 4°C ...
-
bioRxiv - Immunology 2021Quote: ... The protein was concentrated using a 30 kDa MWCO Amicon ultra-15 centrifugal filter (Sigma, cat. no. UFC903024), and stored at −70°C ...
-
bioRxiv - Immunology 2021Quote: ... The eluted protein was incubated with 0.5 mM ADP and 0.5 mM MCC950 (Millipore Sigma, Cat. no: 538120), 0.5 mM dATP or no additives for 30 min on ice ...
-
bioRxiv - Developmental Biology 2021Quote: ... 20 ul protein A/G beads pre-saturated with 5 mg/ml BSA in PBS (Sigma, A-3311) were washed in RIPA buffer and subsequently added to the chromatin/antibody mix at 4°C ...
-
bioRxiv - Developmental Biology 2021Quote: ... The obtained antisera were affinity-purified with recombinant Emi2-His protein electroblotted onto a membrane (Immobilon; EMD Millipore).
-
bioRxiv - Biophysics 2022Quote: ... and subsequently blocked with 0.5 % non-fat dried milk (AppliChem) in TBST using the Snap i.d.® 2.0 Protein Detection System (Millipore). The blot was then incubated for 10 min at room temperature with a 1:1000 dilution of rabbit polyclonal anti-ASIC1a antibody (OSR00097W ...
-
bioRxiv - Neuroscience 2021Quote: ... protein extracts from SCN cultures were examined by SDS-PAGE immunoblotted with anti-UBE3A monoclonal antibody (E8655, Sigma), or anti-FLAG monoclonal antibody (F1804 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Immunoprecipitation of FLAG-AGO2 was performed with Dynabeads Protein G (Life) and anti-FLAG M2 mouse (Sigma, F3165) for 3h at 4ºC with rotation ...