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Citations for Millipore Sigma :
5201 - 5250 of 7633 citations for Yellow Fever Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Collected protein was incubated 1 hour with 200 µL ANTI-FLAG-M2 affinity resin (Sigma-Aldrich; A2220) pre-washed with 3 times with1.0 mL Buffer B (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μL of protein sample was incubated in 57μL sodium acetate buffer pH 5.0 reconstituted in D2O (99.90%, Sigma). Deuterium labelling was performed for 0.5 min ...
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bioRxiv - Cell Biology 2023Quote: ... 1mM DTT) at varying protein or salt concentrations in the presence or absence of 10% PEG6000 (Sigma) and 10% 1,6-hexanediol ...
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bioRxiv - Cell Biology 2022Quote: ... Fusion protein solution was concentrated using a pre-soaked Amicon® Ultra Centrifugal Filter Unit 50MWCO (Millipore) and concentration estimated with NanoDrop2000 UV-Vis spectrophotometer (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were resolved using SDS-PAGE and transferred onto a activated polyvinylidene difluoride (PVDF) membrane (EMD Millipore) at 400 mA for 3 hours using a MiniTransblot Module (BioRad) ...
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bioRxiv - Cell Biology 2022Quote: ... FLAG-tagged proteins were incubated with 30 μl pre-washed anti-FLAG M2 affinity gel (Sigma, #A2220) for 3 h at 4 °C and washed four times with IP wash buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were separated by SDS-PAGE on 12% gels and transferred to PVDF membranes (Immobilon-FL, Millipore). After blocking with for 1 h with blocking buffer (1X TBST with 5% w/v non-fat dry milk) ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were transferred to PVDF membranes and immunoblotted for visualization with Immobilon Western chemiluminescent HRP substrate (Millipore) using a GeneGnome XRQ-chemiluminescence imaging system ...
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bioRxiv - Cell Biology 2023Quote: ... and the His-labelled prey proteins were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen-Millipore, 70954) and incubated in liquid cultures ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted proteins were pooled and concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated sample was loaded onto a HiLoad Superdex S200 (16/600 ...
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bioRxiv - Biochemistry 2023Quote: ... The solubilized proteins were mixed with 20 µL (40 µL slurry) anti-Flag M2 magnetic beads (Sigma) and rolled for 3 hours at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... The RafH protein fraction was pooled and concentrated in a 10 kDa cut-off Amicon filter (Millipore). The protein concentration was checked by measuring absorbance at 280 nm ...
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bioRxiv - Cancer Biology 2023Quote: ... protein samples were separated by SDS-PAGE and transferred to polyvinylidene fluoride membranes (Millipore Sigma, MO, USA). The membrane was stained with Ponceau S to visualize proteins Membranes were blocked with PBS supplemented with 5% nonfat milk before incubation with primary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated by SDS-PAGE and transferred to Immobilon PVDF membranes (EMD Millipore, Billerica, MA, USA). The membrane was incubated for 1 h with antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Total protein was extracted from pili-free cell pellets and whole cell lysates using Bugbuster reagent (Novagen) following the manufacturer’s instructions and quantified using a Bradford assay.
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A crucial role for dynamic expression of components encoding the negative arm of the circadian clockbioRxiv - Biochemistry 2023Quote: ... 2 mgs of total protein were incubated with 20 μL of V5 agarose (Sigma-Aldrich, Catalog # 7345) or FLAG M2 resin (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The protein samples were resolved by 10% SDS-PAGE and transferred to PVDF membranes (Millipore, Billerica, MA) through standard electroblotting procedures ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins were concentrated using centrifugal filters (Amicon Ultra, MWCO 30 kDa and MWCO 10 kDa, Sigma) to 9.96 mg/ml (RTCB ...
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bioRxiv - Immunology 2023Quote: ... Protein expression was induced by the addition of 125 ng/mL doxycycline hyclate (Sigma-Aldrich #D9891-5G) to cells cultured in complete RPMI 1640 containing tetracycline-approved FBS (Sigma-Aldrich #F0926-50ML).
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bioRxiv - Immunology 2023Quote: ... cells were expanded in triple flasks and protein production induced with doxycycline (0.5 µg/ml, Sigma-Aldrich). Supernatants of confluent cells were harvested every 3 days ...
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bioRxiv - Cell Biology 2023Quote: ... total proteins were loaded in the Laemmli sample buffer supplemented with 5% 2-mercaptoethanol (Sigma, catalog #: M3148) to reduce the disulfide bonds ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then treated with 100 µg/mL of the protein synthesis inhibitor cycloheximide (Sigma- Aldrich C4859) for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 10 ng/ml of the recombinant human TNFα protein (Merck-Millipore, Cat# GF023) and the outcome was assessed at RNA or protein levels.
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bioRxiv - Cell Biology 2022Quote: ... The following antibodies were used: PDT-tagged proteins - anti-HA antibody (Sigma, cat# 11666606001, dilution 1:1000), mf-Lon - anti-Flag (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated by SDS-PAGE (7.5%–10% PAG) and transferred onto Immobilon-P membranes (Merck Millipore). Membranes were immunoblotted with the respective antibodies as follows ...
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Dose-dependent effects of histone methyltransferase NSD2 on site-specific double-strand break repairbioRxiv - Cell Biology 2023Quote: Proteins were separated on a by 12% SDS polyacrylamide electrophoresis and transferred to a PVDF membrane (Millipore). Membranes were incubated in 3% skim milk in wash buffer (2 mM Tris-Cl ...
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bioRxiv - Neuroscience 2023Quote: ... Monoclonal antibodies were purified from the cell culture supernatants by affinity chromatography on Protein G Sepharose (Sigma), using 0.1M glycine ...
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bioRxiv - Biochemistry 2024Quote: ... Gfp-tagged protein of interest was detected using a primary mouse anti-Gfp (1:3,000, Millipore/Sigma). An anti-mouse IgG-horseradish peroxidase (HRP ...
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bioRxiv - Plant Biology 2024Quote: ... Labelled proteins were biotinylated with 30 μM azide-PEG3-biotin (Sigma-Aldrich, 2 mM stock in DMSO) using click chemistry as described above ...
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bioRxiv - Systems Biology 2024Quote: ... Proteins were detected by incubation in BCIP/NBT AP substrate buffer (Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Neuroscience 2023Quote: ... The protein sample was concentrated using 100kDa MWCO Amicon® Ultra-15 Centrifugal Filter Unit (EMD Millipore) to a final concentration of ∼0.8mg/ml and filtered by 0.22 µm pore size PVDF membrane prior to deposition on the grid for EM imaging.
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bioRxiv - Microbiology 2023Quote: ... The following antibodies were used for detection of proteins by western blot: anti-M2-FLAG (Sigma, F3165), anti-β-tubulin (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted with a linear gradient of KCl concentrated with a 30,000 MWCO concentrator (Millipore Sigma), and loaded onto a Superdex 200 Increase 10/300 GL and purified using gel filtration buffer (20 mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein lysates for DNA-RNA hybrid pull-down assays were treated with 10 µL RNaseA (Sigma # R6148) or with 10 µL RNaseH (NEB # M0297S ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were detected using commercially available antibodies: anti-Flag mouse Antibody M2 (1:1000, Sigma-Aldrich M8823) and rabbit anti 4R-tau Antibody (1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... The protein expression was induced by addition of 0.1 mM isopropyl-β-D-thiogalactoside (IPTG, Sigma-Aldrich) for 6 hours at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein samples were denatured with 4x Laemmli Sample buffer with 10% Beta-Mercaptoethanol (Sigma-Aldrich, cat: #M6250) followed by 10 min boiling at 95℃ ...
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bioRxiv - Biophysics 2023Quote: ... The protein solution was concentrated using a 30 kDa cutoff centrifugal filter unit (Millipore, Burlington, MA, U.S.A.) and loaded into a Superdex200 10/300 GL gel filtration column (G.E ...
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bioRxiv - Cancer Biology 2023Quote: ... Equivalent amounts of protein were resolved by SDS-PAGE and transferred to polyvinylidene fluoride microporous membrane (Millipore), blocked with 1.5% BSA in H20 containing 0.1% Tween-20 (TBS-T) ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein was separated by 8%–12% SDS-PAGE gels and transferred onto polyvinylidene fluoride membranes (Millipore). Membranes were blocked in 5% BSA in TBS for 1 hour at room temperature and then incubated with primary antibodies overnight at 4C ...
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bioRxiv - Biochemistry 2023Quote: ... The protease inhibitor cocktail (PIC) used in our protein purifications was purchased from Millipore Sigma (catalog #P2714) and prepared as per the manufacturer’s instructions to generate a 1000X stock.
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bioRxiv - Cancer Biology 2023Quote: ... 30 μL of protein extract was mixed with 200 μL of UA (8 M urea (Sigma, U5128) in 0.1 M Tris/HCl pH 8.5 ...
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bioRxiv - Immunology 2023Quote: ... Proteins were separated by SDS-PAGE and then transferred to 0.45 μm PVDF membrane (IPVH00010, Merck Millipore). To keep the amounts of protein equally ...
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bioRxiv - Cancer Biology 2023Quote: ... Fractions containing monodisperse protein were concentrated in a 100 kDa cut-off Amicon ultra centrifugal filters (Millipore) and flash frozen in liquid nitrogen for further assays ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 mg of protein from each sample were incubated with 3 ug of HOXA9 (Millipore # 07-178) or Rabbit IgG Isotype control (Invitrogen # 02-6102 ...
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bioRxiv - Synthetic Biology 2023Quote: ... The pellets were resuspended in 50 μL of BugBuster Protein Extraction Reagent (Millipore Sigma, SKU 70584-4) and vortexed on Benchmark Scientific Incu-Mixer MP at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein bands were visualized on X-ray film by electro-chemiluminescence (Immobilon Western HRP Substrate, WBKLS0500, Millipore). For the isolation of chromatin-enriched proteins ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated with Protein A Agarose/Salmon Sperm DNA and 5 μg human IgG (Sigma, Cat# I4506) or PPAR-γ Antibody (E-8 ...
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bioRxiv - Cancer Biology 2024Quote: ... and up to 100 µg of protein per sample were reduced with 5 mM dithiothreitol (Sigma Aldrich) and incubated in the dark for 30 min ...
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bioRxiv - Immunology 2023Quote: ... The gene encoding the Streptococcus pyogenes M4 protein (uniprot ID P13050) was cloned into pET28_a (+) vector (Novagen). The M4 endogenous N-terminal signal peptide and C-terminal cell wall sorting signal were not included in the construct and a C-terminal hexahistine-tag was added ...