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Citations for Millipore Sigma :
5251 - 5300 of 10000+ citations for Catenin alpha 1 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The membranes were then blocked in 5% milk in Tris Buffered Saline with 1% Tween (TBS-T) overnight at 4° C before being probed overnight at 4° C with Rabbit anti Magi-2 (1:1000, Sigma M4221); Mouse anti-NR1 (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed with PBS and incubated with secondary antibodies at 1:500 dilution (goat anti-rabbit Alexa 594, Sigma Aldrich,) for 1h at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... 2003) and a goat anti-rabbit IgG secondary antibody conjugated with alkaline phosphatase (1:4000, Chemicon®, Sigma-Aldrich, www.sigmaaldrich.com, #AP307P). Detection by chemiluminescence with Immun-Star AP Substrate (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... The blots were washed thrice with TBST buffer and incubated with HRP-conjugated anti-rabbit antibodies (dilution 1:20000; Sigma-Aldrich) at 25°C for 1 h ...
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bioRxiv - Microbiology 2024Quote: ... α-PilT (66) (dilution 1:2000) and α-PilB (66) were used together with horseradish peroxidase-conjugated goat anti-rabbit immunoglobulin G (Sigma) as a secondary antibody (dilution 1:10000) ...
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bioRxiv - Neuroscience 2023Quote: ... Poly-ubiquitinated CHTs were detected by stripping the membranes with Restore Plus buffer (Pierce) and incubating them with 1:2000 diluted rabbit anti-CHT polyclonal antibody (ABN458; EMD Millipore). The resulting chemiluminescent signal was acquired with a Chemidoc Touch Imaging System (Bio-Rad) ...
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bioRxiv - Physiology 2023Quote: ... washed three times with PBS-0.05% tween and followed by incubation with secondary antibodies directed against goat anti-mouse or rabbit HRP conjugated (1:5000, Sigma-Aldrich). The visualization of immunoreactive bands was performed using the enhanced chemiluminescence plus western blotting detection system (GE Healthcare) ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated in anti-TH monoclonal rabbit IgG primary antibody (1:2000 overnight at 4°C; Sigma-Aldrich) in solution with PBS and normal goat serum (NGS) ...
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bioRxiv - Neuroscience 2023Quote: Cytoplasmic and nuclear fractions were then subjected to western blotting and labelled with rabbit monoclonal anti-GAPDH (Sigma, G9545, 1:5,000) antibody and rabbit polyclonal anti-LSD1 antibody (Abcam ...
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bioRxiv - Synthetic Biology 2023Quote: ... in TBS-T for 1 h at room temperature and stained overnight at 4 °C with a rabbit anti-FLAG antibody (1:1000 dilution, Millipore F7425). After removing unbound antibodies by three 5-minute washes with TBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... then washed with PBS again and incubated for 48h with a rabbit anti-TH antibody (1:1000, Millipore Cat# AB152, RRID:AB_390204) at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were washed four times with TBST and incubated with secondary antibody (goat-anti-rabbit or donkey-anti-mouse HRP-conjugated,1:5000 in TBST + 10% dry milk, Sigma Aldrich) for 1 hour at RT ...
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bioRxiv - Genetics 2023Quote: Liver protein isolation and western blots on liver extracts and plasma samples were performed as previously described.17 Primary antibodies to the 2A peptide (1:5000, rabbit, ABS31, Sigma-Aldrich), GFP (1:3000 ...
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bioRxiv - Genetics 2023Quote: ... as previously described.17 The following primary antibodies were used for immunohistochemistry: rabbit anti 2A peptide (1:7500, ABS31, Sigma-Aldrich), rabbit anti Ki67 (1:60 ...
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bioRxiv - Molecular Biology 2023Quote: ... The secondary antibody was the anti-mouse IgG (whole molecule)–peroxidase antibody produced in rabbit (Sigma; cat# A9044; 1:10000 dilution).
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bioRxiv - Cell Biology 2023Quote: ... membranes were washed four times with TBST and incubated with secondary antibody (goat-anti-rabbit or donkey-anti-mouse HRP-conjugated,1:5000 in TBST + 10% dry milk, Sigma Aldrich) for 1 hour at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were incubated in primary antibody solution (1:1000 rabbit anti-GFP or 1:1000 rabbit anti-RNA Pol II CTD repeat YSPTSPS (phospho S5) in blocking buffer (1:10 Western Blocking Reagent (Sigma 11921673001) in PTx) ...
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bioRxiv - Genomics 2023Quote: ... and cells were resuspended in 100 μL dig-wash buffer containing 2 mM EDTA and a 1:50 dilution of rabbit anti-mouse antibody (Sigma, M7023) and incubated at RT for 1 hour with slow rotation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... This was followed by a 1 h incubation at room temperature with anti-mouse or anti-rabbit secondary antibodies conjugated with horseradish peroxidase (HRP; Sigma, A8924). Blots were developed using a Konica SRX101A X-Ray Film Processor.
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed three times with 1× PBS and i ncubated with anti-mouse and anti-rabbit plus PLA probes (PLA kit, Sigma-Aldrich) at 37 °C for 1 h ...
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bioRxiv - Plant Biology 2023Quote: ... Anti-rabbit secondary antibody conjugated with horse radish peroxidase enzyme was used in the ratio of 1:10000 (Sigma Aldrich, USA) and blots were visualized using chemiluminescent substrate as described above.
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bioRxiv - Cell Biology 2023Quote: ... and subjected to PLA with an anti-Rad51 and anti-VE-Cadherin (see Supplementary Table 1) using the Duolink in situ red starter kit (mouse/rabbit) (Sigma-Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... in PBS (BSA/PBS-T) for 1 hour prior to washing with PBS and incubation with primary anti-TH rabbit polyclonal antibody (EMD Millipore) at 1:300 BSA/PBS-T overnight at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Processing of brain and brain sections for TH immunohistochemistry was similar to the protocol described above except for primary (rabbit anti-TH antibody 1/200, Millipore ab152) and secondary (Alexa Fluor 555-conjugated donkey anti-rabbit ...
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bioRxiv - Cancer Biology 2023Quote: ... This was followed by incubation with an anti-EMILIN-1 rabbit polyclonal antibody (x400, HPA002822; Sigma Aldrich, Saint Louis, MO, USA) in Dako REAL Antibody Diluent at 4°C for 24 h ...
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bioRxiv - Developmental Biology 2023Quote: ... the sections were incubated overnight at RT with a rabbit polyclonal anti-pH3 antibody (1:500; Sigma; Cat#: H0412; RRID: AB_477043). Sections were then rinsed 3 times in TBS for 15 min each and incubated for 1 hour at room temperature with a Cy3-conjugated goat anti-rabbit IgG antibody (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were washed in 0.3% PBST several times and then incubated with AlexaFluor goat anti rabbit 633 (1:250, Sigma Cat# SAB4600141) overnight ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... then incubated for 30 min at RT with blocking buffer (1% Bovine Serum Albumin in 1X PBS) and further incubated for at least 1 hour at RT with rabbit anti-hACE2 antibody (SAB 3500978, Sigma-Aldrich) or rabbit anti-TMPRSS2 antibody (BS-6285R ...
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bioRxiv - Neuroscience 2024Quote: ... and probed for iFGF12 and iFGF13 expression using validated (Angsutararux et al., 2023; Nerbonne, unpublished) rabbit polyclonal anti-iFGF12 (Rb-α-iFGF12; 1:500; Sigma) and anti-FGF13 (Rb-α-iFGF13 ...
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bioRxiv - Neuroscience 2024Quote: ... all precipitates were solubilized in SDS sample buffer and detected by western blotting with rabbit anti-FLAG (1:1000; F7425; Sigma-Aldrich), rabbit anti-EZH2 (1:1000 ...
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bioRxiv - Immunology 2024Quote: ... wells were incubated with 10 µg/mL of sera for approximately 1 hr at 20 °C then washed 4 times with PBS/T before the addition of 50 µL of 1:1000 dilution of goat anti-rabbit gamma-chain alkaline phosphatase-conjugated secondary antibody (Sigma-Aldrich) for 1 hr at 20 °C ...
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bioRxiv - Neuroscience 2024Quote: ... dorsal and ventral transverse slices were incubated with the following primary and secondary antibody combinations using the protocol described: PCP4 (1:300, rabbit anti-PCP4 #HPA005792, Sigma-Aldrich)/goat anti-rabbit IgG (Life Technologies ...
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bioRxiv - Microbiology 2024Quote: ... in Tris-buffered saline with Tween 20 (TBST) and incubated with 1:5000 rabbit anti-FtsA or mouse anti-FLAG (F1804, Sigma-Aldrich) antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... The hippocampus was sliced in a transverse orientation and then labeled with PCP4 (1:300, rabbit anti-PCP4 #HPA005792, Sigma-Aldrich) as primary and goat anti-rabbit IgG (Life Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... Kinase assays were performed as described in (20) using 200ng of purified EPHA2 recombinant protein (OriGen Technologies) or indicated concentration of purified Enolase (Sigma Aldrich), in the absence or presence of about 50ng of purified Src-GFP (or Src3A-GFP as indicated ...
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bioRxiv - Molecular Biology 2020Quote: ... coli cells and large quantities of His6 tagged recombinant Nbs were purified with 1ml of HIS-Select Nickel Affinity Gel (Sigma-Aldrich) following periplasmic expression through osmotic shock ...
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bioRxiv - Cell Biology 2021Quote: ... Base media for the culture of mESCs were supplemented with 1000 units/mL ESGRO Recombinant mouse LIF protein (EMD Millipore ESG1107), and 2i small molecule inhibitors ...
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bioRxiv - Biochemistry 2021Quote: ... The membrane was cut in two (one half for the commercial and one half for the recombinant anti-FLAG™-M2) and each half was probed with either commercial (Sigma) or recombinant anti-FLAG™-M2 at 1 µg/mL in PBS for 45 minutes ...
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bioRxiv - Microbiology 2021Quote: ... both Bactrian camel and llama AEC cultures were treated with recombinant universal type I and III IFNs (100 IU/ml; Sigma Aldrich) or recombinant type III IFN (100 ng/ml ...
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bioRxiv - Microbiology 2020Quote: ... strain BL21(DE3) was used for the production of recombinant proteins expressed from the pET series of plasmids (Novagen Sigma-Aldrich). All L ...
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bioRxiv - Microbiology 2020Quote: ... strain BL21(DE3) was used for the production of recombinant proteins expressed from the pET series of plasmids (Novagen Sigma-Aldrich). All L ...
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bioRxiv - Microbiology 2020Quote: ... mouse polyclonal antibody was produced immunizing a mouse with 50 mg of purified recombinant MtEPSPS containing Freund’s incomplete adjuvant (Sigma-Aldrich, USA) (total volume of 100 µL ...
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bioRxiv - Developmental Biology 2022Quote: ... The dish was then incubated with 40 µg/mL mouse E-cadherin recombinant protein (8875-EC-050, Bio-Techne) or 40 µg/mL fibronectin (F1141, Sigma-Aldrich) at 4 □ overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... The chopped and digested pieces were placed in a serrated 60mm petri dishes and supplemented with 10ng/μl each of human recombinant epidermal growth factor (hEGF, Cat # E9644, Sigma-Aldrich), N2 suppliment-1X (Cat # 17502048 ...
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bioRxiv - Cell Biology 2022Quote: ... were used as a substrate for phospho-PKCα in a 25 µl in vitro kinase reaction using 100 ng of active recombinant PKCα enzyme (Millipore-Sigma, MA), 5 µl of a lipid activator (Millipore-Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing the respective recombinant proteins were pooled and concentrated using Amicon® Ultra-15 spin concentrators with 10 kDa cut-off (Merck Millipore). Concentrated protein pools were run on a Superdex 200 Increase 10/300 GL ...
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bioRxiv - Immunology 2020Quote: ... extracellular Cn were removed by washing 2X with PBS and the macrophages were cultured for a further 24 h in fresh growth media containing either IFNγ to maintain the M1 polarization state or 100 ng/mL recombinant interleukin-4 (IL-4; Sigma-Aldrich, St ...
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bioRxiv - Microbiology 2021Quote: ... Sublines expressing a doxycycline-inducible BZLF1 transactivator were produced by culturing 106 LCL cells with the recombinant lentivirus in presence of 8 μg/ml polybrene (TR-1003-G, Sigma-Aldrich) for 24 hours followed by replacement of the infection medium with fresh complete medium ...
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bioRxiv - Microbiology 2019Quote: ... we performed growth curves in YPD liquid medium containing different concentrations of Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) in a 96-well plate (Costar ...
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bioRxiv - Biochemistry 2019Quote: ... recombinant mTLR9-ECD was titrated into a solution of oligonucleotide labeled at the 5’ end with Alexa 488 Fluor (Sigma-Aldrich). The oligonucleotide concentration ...