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Citations for Millipore Sigma :
5201 - 5250 of 10000+ citations for Catenin alpha 1 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... A second incubation of 1 hour at room temperature was then performed with goat anti mouse Alexa Fluor 555 and anti-rabbit FITC conjugated (1:400, F7512, Sigma Aldrich). For all the labelling ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies were used at the following dilutions: horseradish peroxidase coupled secondary antibodies (α-mouse and α-rabbit, Sigma, 1:20,000), anti-mouse Oregon Green (Molecular Probes ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were washed five times with TBS and incubated with secondary antibody (goat-anti-rabbit HRP-conjugated,1:2000 in TBS + 5% dry milk, Sigma Aldrich) for one hour at RT ...
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bioRxiv - Biochemistry 2022Quote: ... in PBS for 1 hour at room temperature before probing with anti-Hs haemoglobin (rabbit IgG, 5 µg/mL, Sigma Aldrich), anti-HA (mouse monoclonal Clone HA-7 ...
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bioRxiv - Neuroscience 2022Quote: ... followed by an overnight incubation at 4°C with rabbit anti-HA antibody (1:1000, Cat no. H6908, Sigma-Aldrich, USA). For c-Fos ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated in PBS containing 0.5% normal donkey serum and 0.03% Triton X-100 for 1 h at room temperature (RT, 23 ± 7□) and then stained with primary antibodies overnight at 4□ (rabbit anti-c-Fos, 1:5000, Sigma-Aldrich; rat anti-CTIP2 ...
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bioRxiv - Physiology 2022Quote: ... Membranes was incubated with the primary antibodies against target proteins: rabbit polyclonal anti-TSG 101 (T5701, Sigma-Aldrich Co., 1:200), rabbit polyclonal anti-CD63 (SAB4301607 ...
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bioRxiv - Molecular Biology 2022Quote: ... Western blots were probed with the following antibodies at the indicated concentrations: rabbit polyclonal anti-FLAG (Millipore Sigma, F7425; 1:1,000), mouse monoclonal anti-FLAG M2 (Millipore Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated overnight at 4°C with the primary antibodies (Abs): 1:400 rabbit anti-GFAP (EMD Millipore catalog#ABS5804); 1:1000 rabbit anti-Iba1 (Wako catalog#019-19741) ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated with one or more of the following primary antibodies (rabbit anti-TH, 1:2000, AB152, EMD Millipore; guinea pig anti-VGLUT2 ...
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bioRxiv - Immunology 2021Quote: ... antibody diluted 1:1000 in blocking solution or rabbit Anti-Monkey IgG (whole molecule)-Peroxidase antibody (Sigma-Aldrich Cat# A2054, RRID:AB_257967) were added to each well ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were collected in PBS in 24-well plates and processed for free-floating immunocytochemistry using primary polyclonal antibodies of phosphorylated Tau pSer214 (, rabbit, 1:1000 Sigma, USA) and NOS (Cayman Chemical,1:1000 ...
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bioRxiv - Developmental Biology 2021Quote: ... the membrane was then incubated overnight at 4°C with primary antibodies: 1∶15000 rabbit anti-V5 antibody (SIGMA, Cat. # V8137), and 1:12000 mouse anti-α-Tubulin antibody (SIGMA ...
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bioRxiv - Neuroscience 2021Quote: The following primary antibodies were used for staining: anti-phospho-CREB (ser133) antibody (1:1000 dilution, rabbit, Millipore Sigma, 06-519), Phospho-Myosin Light Chain 2 (Ser19 ...
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bioRxiv - Neuroscience 2021Quote: ... washed 3 x 10 min in PBS-TX (0.2% Triton X-100 in 0.1M PBS) and incubated with the primary antibody rabbit anti-CTB overnight (1:3000) (Sigma-Aldrich, UK). Sections were washed for 3 x 10 min (PBS-TX ...
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bioRxiv - Neuroscience 2021Quote: ... permeabilized with 0.3% Triton X-100 in PBS (PBST) for 10 minutes and incubated with rabbit primary antibody against TH (1:1000, AB152; Millipore/Sigma) at 4°C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then blocked for 1h in PBST with 5% of Bovine Serum Albumin (BSA) and incubated overnight with rabbit anti-TH antibody (1:1000, Millipore, AB152) diluted in PBST with 2% of BSA ...
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bioRxiv - Genetics 2022Quote: ... Bb was cultured at 37ºC in Barbour-Stonner-Kelly with 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer media (BSK-H) complete with 6% rabbit serum (Millipore Sigma) and 1% Amphotericin B (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: The staining of cells and tissues was performed with the following primary antibodies: rabbit polyclonal anti-CEP164 (1:1,000; EMD Millipore, ABE2621), mouse monoclonal acetylated-α-tubulin (1:1,000 IF ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were subsequently washed with PBS and incubated for 2 h with the proper secondary antibodies (1:300, goat anti-rabbit Alexa-Fluor® 647, AP187SA6, Merck Millipore; 1:300 ...
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bioRxiv - Plant Biology 2019Quote: ... before being rinsed twice for 15 min each in PBS/BSA and incubated for 15 min in a 1/100 dilution (in PBS/BSA) of biotinylated goat anti-rabbit secondary antibody (Sigma Aldrich). Slides were rinsed as before and incubated with a 1/100 dilution (in PBS/BSA ...
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bioRxiv - Neuroscience 2019Quote: ... cells were blocked-permeabilized in 5% gelatin from cold water fish skin and 0.1% triton X-100 for 1 h followed by incubation with primary antibodies (mouse anti-acetylated tubulin, and rabbit anti-Neurofilament heavy chain, Sigma-Aldrich) overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Detection was carried out by incubating with a 1:5000 dilution of a goat anti-rabbit antibody conjugated to peroxidase (Sigma Aldrich). For tagged proteins detection ...
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bioRxiv - Cell Biology 2020Quote: ... Detection was carried out by incubating with a 1:4000 dilution of a rabbit anti-rat antibody conjugated to peroxidase (A5795, Sigma-Aldrich). The membranes were then developed with the ECL Plus™ Western blotting detection system (PerkinElmer Life Sciences ...
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bioRxiv - Plant Biology 2021Quote: ... The PVDF membrane was then incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit (diluted 1:10,000; Sigma-Aldrich, Stockholm, Sweden) for 1 h at room temperature followed by three washes with TBST and detection using a Agrisera ECL kit (catalogue no ...
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bioRxiv - Pathology 2021Quote: ... cryosections were transferred to TBS and incubated for 1 h at 37 °C with anti-mouse PLUS and anti-Rabbit MINUS PLA probes (Sigma-Aldrich). Then ...
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bioRxiv - Developmental Biology 2020Quote: ... blocked with 3% BSA/TBST for 30 min and incubated with rabbit anti-Sox9 polyclonal antibody (1:500, EMD-Millipore, AB5535), rat anti-endomucin (Emcn ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissue was then washed in PBS containing 0.03% tween-20 for two minutes following overnight incubation at 4°C with anti-versican GAGβ (1:100 dilution, rabbit source, Millipore AB1033). Tissue was then washed three times in PBS containing 0.03% tween-20 for fifteen minutes followed by secondary antibody staining for one hour at room temperature (anti-rabbit Alexafluor 488 ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated for 90 min with either horse-radish peroxidase (HRP) conjugated goat anti-rabbit IgG (1:10000; EMD Millipore) or HRP conjugated goat anti-mouse IgG (1:10000 ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were washed five times with TBS and incubated with secondary antibody (goat-anti-rabbit/mouse HRP-conjugated,1:2000 in TBS + 5% dry milk, Sigma Aldrich) for 45 minutes at RT ...
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bioRxiv - Microbiology 2020Quote: ... grids were incubated for 1 hour 37 °C with a 1:2000 dilution of a 10 nm gold-labelled goat anti-rabbit IgG (G7277; Sigma Aldrich), washed with 1x PBS ...
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bioRxiv - Immunology 2021Quote: ... was used at a 1:5000 dilution (T84) or horseradish peroxidase-conjugated goat anti-rabbit IgG secondary antibody (A0545, Sigma-Aldrich) at 1:2000 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... and blocked with 2% donkey serum and then immunostained using rabbit antibody against Cx43 (Figure 6A, Sigma, cat# C6219, 1:500) or Alpaca antibody against Green Fluorescent Protein conjugated to Alexa Fluor 488 (also labels EBFP2 ...
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bioRxiv - Immunology 2020Quote: ... were washed twice with 1 mL of buffer and sensitised with a 1/1000 dilution of rabbit anti-sheep red blood cell stroma antibody (Sigma; S1389). After a 30 °C / 30 minutes incubation ...
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bioRxiv - Genomics 2021Quote: ... The membranes were incubated overnight at 4°C with a 1:5000 dilution of monoclonal rabbit anti-HA antibodies (Sigma-Aldrich) or 1:5000 dilution of monoclonal rabbit anti-FLAG antibodies (Sigma-Aldrich).
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bioRxiv - Cell Biology 2021Quote: ... followed by a goat anti-rabbit secondary antibody conjugated with Cy3 for 1hr at room temperature (1:500, Millipore, Cat. #AP132C).
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed in 1xTBS and then in BS before probing with anti-rabbit antibody conjugated to Horse Radish Peroxidase (HRP) (1:10,000 in BS, Sigma, goat, A9169) for 60 minutes at RT ...
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bioRxiv - Neuroscience 2020Quote: ... Tissues were then incubated overnight with the following cocktail of primary antibodies to presynaptic components diluted in blocking solution with rocking: rabbit anti-NF 200 (1:200; Sigma, N4142), mouse anti-synaptophysin (1:300 ...
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bioRxiv - Neuroscience 2022Quote: ... The sections from the animals of different treatment groups were washed using PBS and permeabilized with 0.3% Triton X-100 and followed by incubation overnight at 4°C with the following primary antibodies:: rabbit anti-NeuN (1:100, Sigma-Aldrich), mouse anti-GFAP (1:500 ...
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bioRxiv - Biochemistry 2022Quote: ... The following secondary antibodies diluted to 1:6000 in 3% milk and applied for two hours at room temperature: goat anti-Rabbit IgG peroxidase (Sigma #A0545) and goat anti-mouse IgG peroxidase (Sigma #A0168) ...
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bioRxiv - Immunology 2022Quote: ... Membranes were then washed with PBS-T and incubated with anti-rabbit or anti-mouse peroxidase-labeled antibodies (1:10000, Sigma-Aldrich). After extensive washing with PBS-T ...
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bioRxiv - Genetics 2022Quote: ... TAP-tagged Rrp45 protein was detected with peroxidase anti-peroxidase (PAP) soluble complex antibody produced in rabbit (1:5000, Sigma-Aldrich). The 3-phosphoglycerate kinase (Pgk1 ...
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bioRxiv - Neuroscience 2022Quote: ... the samples were incubated in 1:30 rat anti-DN-cadherin (DN-EX #8, Developmental Studies Hybridoma Bank) and 1:500 rabbit anti-GABA (A2052, Sigma-Aldrich) or 1:200 rabbit anti-Lucifer yellow (A5750 ...
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bioRxiv - Neuroscience 2022Quote: ... brains were first cut into 50 um slices and store at 4C in .1% sodium azide PBS before undergoing NeuN staining procedures using Alexa Fluor 488 Conjugate ABN78A4 Anti-NeuN (rabbit) antibody (Sigma-Aldrich). Slices were washed 3 times for 10 minutes with 1x PBS and pre-incubated in 10% Horse Serum and 0.3% Triton-X-100-PBS with 1% BSA for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Immunofluorescence was performed using an automatic liquid handling dispenser (Personal Pipetter, ApricotDesigns) using Rabbit anti-human SYNAPSIN1 (1:1000, Millipore, AB1543) and Chicken anti-human MAP2 (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: ... TAP- and HTP-tagged proteins were detected after a unique incubation of the membranes with Peroxidase Anti-Peroxidase Complex produced in rabbit (1:2,000; Sigma, Cat# P1291). Chemiluminescence signals were revealed on an Amersham™ Imager 600 (GE Healthcare ...
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bioRxiv - Developmental Biology 2022Quote: ... Specific protein detection was performed using diluted primary antibodies as follows: anti-NDRG1 @ 1:5000 (Sigma-Aldrich, catalog# HPA006881, rabbit polyclonal); anti-GAPDH @1:5000 (GeneTex ...
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bioRxiv - Plant Biology 2022Quote: ... Slides were then washed five times with PBS-BSA at 25°C followed by incubation in a 1:200 dilution of the tetramethylrhodamine-isothiocyanate-labelled goat anti-rabbit antibody (TRITC, Sigma-Aldrich) and fluorescein isothiocyanate-labelled goat anti-mouse antibody (FITC ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue was permeabilized/blocked in a solution containing 1% Triton-X and 10% NGS in PBS for 30 min and then incubated with an anti-HNE-Michael adducts rabbit polyclonal antibody (1:150 dilution, EMD Millipore) in a 10% NGS ...
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bioRxiv - Neuroscience 2022Quote: ... The secondary substrate was horse radish peroxidase tagged anti-rabbit secondary antibody and anti-goat secondary antibody (1:2500, EMD Millipore). The bands were detected using a chemiluminescent substrate (SuperSignal West Pico ...