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Citations for Millipore Sigma :
5151 - 5200 of 5739 citations for Heme Assay Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: Free fatty acid (FFA) content was quantified in mouse plasma samples using the Free Fatty Acid Quantitation Kit (Sigma, #MAK0044) in line with the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... GLP-1 content in the supernatant of T0 and T20 samples was assessed using a Glucagon-Like Peptide-1 (GLP-1) Total ELISA KIT (96-Well Plate Cat. # EZGLP1T-36K, EZGLP1T-36BK, Millipore).
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bioRxiv - Cell Biology 2023Quote: ... histone H1.2 knockout heterozygous (Het) and homozygous (KO) mice are isolated using the Nuclei Isolation ‘Kit Nuclei EZ Prep’ (NUC101, Sigma). Then the nuclei are treated with 500 µM MNNG with fresh-made NAD+ (N0632 ...
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bioRxiv - Genomics 2023Quote: To obtain sufficient nuclei to run the same suspension across both fixed kits we used EZ lysis buffer (Sigma NUC101), at the expense of greater debris ...
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bioRxiv - Immunology 2023Quote: The serum alanine aminotransferase (ALT) activity was analysed using a commercially available kit according to the manufacturer’s guidance (Sigma-Aldrich) and a Spark multimode microplate reader (Tecan ...
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bioRxiv - Immunology 2023Quote: ... the proteins of the parietal-cell cytosol and membrane/organelle fractions were extracted with the ProteoExtract Subcellular Proteome Extraction Kit (Millipore) to exclude the nucleic and cytoskeletal fractions ...
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bioRxiv - Plant Biology 2023Quote: ... bacteria were harvested by centrifugation and pellets were resuspended in 5 ml of binding buffer (His-bind buffer kit (Millipore), proteins were extracted using a Constant cell disrupter system (Constant Systems ...
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bioRxiv - Biochemistry 2023Quote: Apoptotic cell death was detected using the The ApopTag Plus Fluorescein In Situ Apoptosis Detection Kit (Sigma, S7111, supplemental methods).
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bioRxiv - Cell Biology 2023Quote: ... Mycoplasma contamination of cells was tested and ruled out with the LookOut Mycoplasma Detection kit (Sigma-Aldrich; catalog number: MP0035).
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bioRxiv - Bioengineering 2022Quote: ... The cell line was tested as being negative for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) and Jumpstart™ Taq DNA Polymerase (Sigma-Aldrich).
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bioRxiv - Cell Biology 2022Quote: ... growth and pattern of metastasis in vivo and routinely screened for Mycoplasma (LookOut® Mycoplasma PCR Detection kit, Sigma-Aldrich). None of the cells used are listed in the International Cell Line Authentication Committee (ICLAC ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed cells were stained either for ALP activity using an ALP detection kit according to the manufacturer’s instructions (Sigma-Aldrich) or with crystal violet ...
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bioRxiv - Cell Biology 2023Quote: ... NPCs were differentiated to astrocytes using the Stem Cell Technologies STEMdiff astrocyte differentiation and maturation kits during which they were split weekly with Accutase (Millipore).
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bioRxiv - Cell Biology 2023Quote: ... 1 μM of carbonylcyanide-4-(trifluoromethoxy)-phenylhydrazone (FCCP) (Seahorse XF Cell Energy Phenotype Test Kit from Agilent or Sigma Aldrich), and 1 μM of antimycin A and rotenone (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: Ratios between the oxidized form NAD+ and the reduced form NADH were measured using NAD+/NADH quantification kit (MAK037, Sigma) with whole cell lysates of wild type S ...
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bioRxiv - Genomics 2023Quote: ... The material was ground with mortar and pestle and the RNA was extracted with the Spectrum Plant Total RNA kit (Sigma) and treated with DNAse I amplification grade (Sigma) ...
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bioRxiv - Genomics 2023Quote: ... immunoprecipitated NS from the four S-phase fractions collected by flow cytometry or from an asynchronous cell population were amplified by whole-genome amplification (GenomePlex Complete Whole Genome Amplification kit #WGA2; Sigma) according to the manufacturer’s recommendations to obtain sufficient DNA amount ...
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bioRxiv - Plant Biology 2022Quote: ... 3-6 µg total protein was derivatized with DNPH and subsequently detected with an anti-DNP antibody according to the manufacturer’s instruction of the OxyBlot Protein Oxidation Detection Kit (S7150, Sigma-Aldrich). Equal amounts of the derivatized protein samples were employed as loading control on a separate SDS-gel and stained with Coomassie or silver staining.
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bioRxiv - Genomics 2022Quote: ... Genome extraction was then performed using the GenElute Bacterial Genomic DNA (gDNA) extraction kit (Sigma-Aldrich, St. Louis, MO, USA) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was extracted using GenEluteTM Mammalian Total RNA Miniprep Kit and On-Column DNase I Digestion Set (both Sigma-Aldrich) and a total of 9 samples fulfilling the submission requirements (0.5-1.0μg total RNA in nuclease free dH2O with A260/A280 and A60/A230 ratios above 1.8 ...
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bioRxiv - Genomics 2023Quote: ... The bisulfite-treated DNA fragments were ligated with a nine-nucleotide adapter and amplified using a GenomePlex Single Cell Whole Genome Amplification Kit (Sigma) for three rounds ...
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bioRxiv - Neuroscience 2023Quote: ... all cultures were monthly tested for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (Sigma, cat. no MP0035-1KT).
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bioRxiv - Bioengineering 2023Quote: ... (Shrock, Shrock, and Elledge 2022) The ligated inserts were packaged with the T7Select packaging kit (Millipore Sigma, St. Louis, MO) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ER was purified according to the protocol optimised by combining the traditional microsome-based density gradient isolation method (Endoplasmic Reticulum Isolation Kit developed by Sigma) with the cell surface biotinylation reaction method (developed and optimised by Pierce) ...
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bioRxiv - Biochemistry 2023Quote: ... A standard cell-free reaction (2-3 ml of RM) was carried out in 50 ml tubes using a Pur-A-Lyzer Maxi dialysis kit (#PURX35050, Sigma). The TrkBtm was expressed as a precipitate ...
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bioRxiv - Immunology 2023Quote: ... EasySep™ Human NK Cell Isolation Kit) and were frozen down in CS10 CryoStor cell cryopreservation media media (Sigma-Aldrich).
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bioRxiv - Systems Biology 2023Quote: ... The amount of glucose remaining at each time point was measured by using a glucose-oxidase-peroxidase reaction kit (Sigma). Glucose phosphorylation activity was evaluated from the total amount of glucose consumed per unit of time.
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bioRxiv - Immunology 2023Quote: ... Neisserial genomic DNA was extracted from cell suspensions of overnight culture plates using the GenElute Bacterial Genomic DNA Kit (Sigma). Genomic libraries were generated using Illumina Nextera DNA Flex Library Prep according to the manufacturer’s instructions and sequenced on Illumina MiSeq 2×250bp paired end platform ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The concentration of tGSH (nM/mg of erythrocyte pellet) was assayed using a commercial kit (Sigma-Aldrich, St Louis, MO) and according to (Galván and Alonso-Alvarez ...
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bioRxiv - Cell Biology 2023Quote: ... Cytokine analysis was performed by Cincinnati Children’s Hospital Research Flow Cytometry Core per kit instructions (Milliplex MAP human protein panel, Millipore-Sigma).
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... The quantity of extracellular vesicles was evaluated by measuring the protein concentrations that were measured using Bicinchoninic Acid Kit (Sigma). Cardiomyocytes or spheroids were treated with pelleted vesicles corresponding to 0.1 mg of proteins per 25 000 cells every two days from day 10 until day 24 after the initiation of differentiation.
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bioRxiv - Pathology 2023Quote: Genomic DNA was isolated from mouse tail biopsies using the GenElute Mammalian Genomic DNA extraction kit (Millipore-Sigma, G1N350-1KT) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: Genomic DNA was isolated from mouse tail biopsies using the GenElute Mammalian Genomic DNA extraction kit (Millipore-Sigma, G1N350-1KT) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Glycerolipid content of male and female whole bodies and female ovaries and fat bodies was measured 12 days after dsGFP and dsXPO1 microinjection using the colorimetric Serum Triglyceride Determination kit (Sigma, Triglyceride Reagent T2449 ...
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bioRxiv - Neuroscience 2023Quote: ... Acyl-ghrelin concentrations in mouse plasma samples were determined by commercial ELISA kits (EMD Millipore, Billerica, MD; Catalog #EZRGRA-90K) (91).
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bioRxiv - Microbiology 2023Quote: ... Regular testing for mycoplasma contamination was performed to confirm contamination free culture using a PCR-detection kit (Sigma-Aldrich/Merck). The details of cell lines are provided in key resources table ...
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bioRxiv - Developmental Biology 2023Quote: ... The resultant samples were load on SDS-PAGE and subjected to pIMAGO phospho-protein detection kit with fluor-680 (Sigma) detection kit and LI-COR Odyssey platform for western blot analyses of phosphorylated proteins (Millipore Sigma) ...
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bioRxiv - Genomics 2023Quote: ... Cells were validated by STR analysis and regularly confirmed to be mycoplasma free by Venor GeM Mycoplasma Detection Kit (Milipore Sigma). 293T cells were cultured at 37 C ...
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bioRxiv - Microbiology 2023Quote: ... The insoluble matter was removed by centrifugation 10000×g for 5 min at 4 °C and protein was estimated using BCA kit (Sigma) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... and DNA was extracted from a 500 mg crushed root sample using GenEluteTM Soil DNA Isolation kit (Sigma-Aldrich, Canada) with slight modifications ...
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bioRxiv - Cancer Biology 2023Quote: ... The abundance of cytokines was determined using a MILLIPLEX Mouse 32-Plex Cytokine Panel 1 kit (Millipore # MCYTMAG-70K-PX32) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... neurons were fixed at 4 days in vitro (DIV4) with 4% PFA and the Duolink PLA kit was employed as previously described 7 following the manufacturer’s (Sigma-Aldrich) protocol 45.
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bioRxiv - Microbiology 2023Quote: The subtype of the mAb was IgG1 as determined using a subtype identification kit (Sigma-Aldrich, St. Louis, MO, USA). Non-immunoglobins in the ascites were removed by precipitation with caprylic acid ...
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bioRxiv - Neuroscience 2024Quote: Binding of cGAS and cytoplasmic mtDNA in human and mouse brain sections was determined by a PLA kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... profiling at Broad between January and December 2018 and were routinely tested for mycoplasma using LookOut Mycoplasma PCR Kit (Sigma)
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bioRxiv - Microbiology 2024Quote: ... The plasmid containing the desired complement was extracted from the confirmed colonies using GenElute™ Plasmid Miniprep Kit (Sigma-Aldrich). The plasmid containing the required complement was then electroporated into the phage-resistant mutants ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... DNA was extracted from the euthanized embryos using Extract-N-Amp Tissue PCR Kit (Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Mycoplasma contamination was tested to confirm contamination-free cell culture using a PCR-detection kit (Sigma-Aldrich, cat no# MP0035).
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bioRxiv - Plant Biology 2024Quote: All samples were homogenised using Geno/Grinder 2010 (SPEX SamplePrep) and total RNA was isolated from homogenised samples using the Sigma Spectrum Plant Total RNA kit (Sigma) supplemented with the On-Column DNase I Digestion step to remove genomic DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... and a random RT-PCR amplification was performed using the TransPlex® Complete Whole Transcriptom Amplification Kit WTA2 (Merck Millipore) following the manufacturers’ instructions ...