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Citations for Millipore Sigma :
5051 - 5100 of 5739 citations for Heme Assay Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The proximity ligation reaction and amplification of signal were performed according to the manufacturer’s protocol using the Duolink Detection Kit with PLA PLUS and MINUS probes for mouse and rabbit antibodies (Duolink, Sigma). DAPI stain was used to detect cell nuclei ...
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bioRxiv - Microbiology 2020Quote: ... validated as hex1 deletion mutants by spore PCR and Southern blot analysis (DIG DNA Labeling and Detection Kit, Sigma-Aldrich), and stored as 25% glycerol stocks at −80 °C.
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bioRxiv - Genomics 2020Quote: ... nuclei were isolated by gently homogenizing tissue with a pestle in cold Nuclei Isolation Buffer from a Nuclei PURE Prep Kit (Sigma). Nuclei were pelleted and supernatant removed before being digested by incubating in 70 µl PBS ...
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bioRxiv - Neuroscience 2021Quote: ... plasma was isolated from tail-blood and measured (3 μL in technical duplicates) using the Cholesterol Quantitation Kit (Sigma, MAK043) or the Triglyceride Quantification Colorimetric/Fluorometric Kit (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines were routinely tested for mycoplasma contamination on a monthly basis using the LookOut Mycoplasma PCR Detection Kit (Sigma). Any cells yielding positive mycoplasma results were immediately discarded.
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bioRxiv - Cancer Biology 2021Quote: ... The PLA was performed using reagents supplied in the Duolink in situ Red PLA Mouse/Rabbit kit (Sigma-Aldrich, DUO92101) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Control line C7 was derived from a 41-year-old-female and reprogrammed using the Simplicon™ reprogramming kit (Millipore). Overexpression of the Yamanaka factors was introduced by a non-integrative ...
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bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Total RNA was extracted from lung tissue or sorted cells as indicated in the text with Total RNA purification kit (Sigma) and treated with DNase I (Invitrogen) ...
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bioRxiv - Bioengineering 2021Quote: ... total DNA content was isolated from the 3D constructs at 14 and 21 days using a DNA extraction kit (Sigma). The isolated DNA contents were collected and quantified by using Nano drop (Thermo Scientific-2000).
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bioRxiv - Biochemistry 2021Quote: Total RNA was isolated using the Trizol method and mRNAs were isolated with the GenElute™ mRNA Miniprep Kit (Sigma). The concentration and purity of the mRNA were measured using a NanoDrop 2000 ...
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bioRxiv - Cancer Biology 2021Quote: Detection of apoptosis in vivo was performed using the ApopTag Peroxidase In Situ Apoptosis Detection Kit (Millipore, Billerica, MA, USA) according to the manufacturer’s instructions.
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bioRxiv - Biophysics 2020Quote: ... Cell viability under 42 h compound treatment was determined via the Cell Counting Kit-8 (CCK-8, Sigma-Aldrich #96992) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... The Millipore Mouse Cytokine 32-plex kit was used to measure the serum concentration of 32 cytokines (Cat# MCYTMAG-70K-PX32, Millipore). xPONENT (Luminex ...
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bioRxiv - Microbiology 2022Quote: ... trachomatis serovar L2 stocks were routinely tested for Mycoplasma contamination using the Mycoplasma PCR detection kit (Sigma, St. Louis, MO).
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bioRxiv - Cancer Biology 2022Quote: ... 1×105 cells were used to measure NADPH and NADP+ levels using a NADP+/NADPH colorimetric quantitation kit (Sigma-Aldrich).
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bioRxiv - Cell Biology 2022Quote: Immunoprecipitation was performed using SRF or MKL1 antibody or control rabbit IgG with Magnetic Protein A/G beads (Magna ChIP A/G kit, Millipore), as described previously (Chatterjee et al. ...
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bioRxiv - Neuroscience 2020Quote: ... All DNA fragments were cleaned-up with GenElute™ PCR Clean-Up Kit (Sigma Aldrich Co., St. Louis, MO, USA) prior to PCR analysis ...
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bioRxiv - Physiology 2020Quote: ... Systemic levels of vascular markers were evaluated at the end of the Fc-GDF15 and vehicle treatment of obese ZSF1 rats by using multiplex MILLIplex kits (Millipore). The rat vascular injury panels included VEGF ...
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bioRxiv - Physiology 2020Quote: ... Kidney injury markers KIM-1 and NGAL were evaluated in serum from 20-week-old lean and obese ZSF1 rats by multiplex MILLIplex kits (Millipore). Serum NT-proBNP and rat cardiac injury markers (fatty-acid-binding protein 3 [FABP3] and myosin light chain 3 [Myl3] ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were allowed to recover for 36 hours after media change and Nuclear-Complex Co-IP was performed using commercially available Nuclear-Complex Co-IP kit from ActiveMotif(54001) with manufacturer’s recommended protocol using mouse Anti-Flag (Sigma F3165) or mouse Anti-His (Abcam 18184 ...
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bioRxiv - Pathology 2020Quote: ... and methyl cytosine levels assessed by a specific primary and secondary HRP-conjugated antibody system following the manufacturer’s instructions (Imprint® Methylated DNA Quantification Kit-Sigma). Following development with TMB ...
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bioRxiv - Bioengineering 2020Quote: ... The hiPSC line LUMC0072iCTRL01 was obtained from the hiPSC core facility at the Leiden University Medical Center where they were generated from fibroblasts using the Simplicon RNA reprogramming kit (Millipore).
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bioRxiv - Molecular Biology 2020Quote: ... The cloning was performed according to the manufacturer’s protocol using the T7Select® 415-1 Cloning Kit (Millipore, 70015-3) with 1 μL of annealed oligonucleotides.
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bioRxiv - Cancer Biology 2020Quote: The cell-lines were confirmed to be mycoplasma negative using the Venor™GeM Mycoplasma Detection PCR kit (MP0025; Sigma).
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bioRxiv - Developmental Biology 2021Quote: ... the cells were fixed with 4% paraformaldehyde and stained for TRAPase activity using a TRAPase staining kit according to the manufacturer’s protocols (Sigma-Aldrich, Acid phosphatase leukocyte ...
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bioRxiv - Microbiology 2020Quote: ... RNA was converted to cDNA using the First Strand cDNA synthesis kit for reverse transcription (avian myeloblastosis virus [AMV]; Millipore-Sigma). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Microbiology 2021Quote: Isotyping of the monoclonal antibodies and polyclonal sera responses was performed using the Mouse Monoclonal Antibody Isotyping Kit (Sigma-Aldrich), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was extracted from leaf tissue using the Spectrum Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA) following Protocol A.
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bioRxiv - Immunology 2020Quote: ... at a density of 50,000 cells/well for indicated time were fixed in 2.5% Glutaraldehyde and Tartrate-Resistant Acid Phosphatase in osteoclasts were stained using TRAP staining kit according to manufacturer’s instruction (Sigma-Aldrich).
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bioRxiv - Developmental Biology 2021Quote: Cell death was assessed using the TdT-mediated dUTP nick end-labeling (TUNEL) method (ApopTagPeroxidase In Situ Apoptosis detection kit, Millipore) as per manufacturers’ instructions ...
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bioRxiv - Neuroscience 2021Quote: ... was isolated from 2 × 106 MØP cells in accordance with the instructions of the GenElute™ Mammalian Genomic DNA Miniprep Kit (Sigma). Separate PCR reactions were prepared using the GoTaq® Master Mix (Promega ...
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bioRxiv - Genetics 2021Quote: ... Purification of genomic Polymerase Chain Reaction (PCR) amplified DNA was performed with the GenElute PCR Clean-Up kit (Sigma-Aldrich) or the GeneJET PCR Purification Kit (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2020Quote: Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) was performed on whole-mount larvae (ApopTag Fluorescein In Situ Apoptosis Detection Kit; Millipore) as previously described (Lambert et al. ...
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bioRxiv - Neuroscience 2020Quote: APOE genotype was determined by extracting genomic DNA from participants’ blood samples using a GenElute Blood Genomic DNA Kit (Sigma). After confirming concentration and quality by Nanodrop ...
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bioRxiv - Biophysics 2021Quote: ... Cells were harvested by centrifugation at 6000xg for 10min and the pellet was re-suspended in LB/kanamycin for plasmid extraction and purification using a GenElute plasmid miniprep kit (Sigma).
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bioRxiv - Biochemistry 2021Quote: ... Experimental molecular weights were calculated using calibration standards from the Gel Filtration Molecular Weight Markers Kit for molecular weights 12-200 kDa (Sigma) and the Gel Filtration Calibration Kit for molecular weights 43-669 kDa (Cytiva) ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR fragments were analyzed using Agarose Gel Electrophoresis and purified from Gel using the GenElute Gel Extraction Kit (Sigma, NA1111) or MinElute Gel Extraction Kit (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: ... Lysate collection and RNA immunoprecipitations were performed using the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore #17-700) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Glycaemia was measured using a glucometer and insulin levels using the Milliplex Map Kit (Mouse Metabolic Magnetic Bead Panel. MMHMAG-44K, Millipore) Milliplex Map Kit ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Citrulline-containing proteins were modified on the membrane and detected using the anti-modified citrulline detection kit (Millipore, 17-347) according to manufacturer’s instructions.
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bioRxiv - Genomics 2020Quote: ... Cells were harvested at μmax and genomic DNA was isolated using GenEluteTM Bacterial Genomic DNA Kit (NA2120-1KT, Sigma-Aldrich) using the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... CRISPR-targeted regions were amplified with MiSeq-compatible gene-specific primers containing Read1 and Read2 adaptor sequences (IDT) using KOD DNA polymerase Hot start kit (TOYOBO/Millipore). The second PCR was performed with index adaptor primers compatible with Ilumina MiSeq (i5 and i7 primers ...
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bioRxiv - Cancer Biology 2021Quote: Gene expression was measured by quantitative RT-PCR and normalized to β-actin8,48 The SYBR Green quantitative RT-PCR kit (Sigma) was used with the following primers ...
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bioRxiv - Microbiology 2020Quote: ... From these fixed cells the manufacturer’s Duolink PLA Immunofluorescence protocol was followed closely using the Duolink In Situ Red Starter Kit Mouse/Rabbit (Millipore Sigma). Briefly ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A first purification step was performed using immobilized metal affinity chromatography with a His-bind resin column (His-Bond kit, Novagen) to recover PGM variants ...
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bioRxiv - Neuroscience 2020Quote: ... They were blocked by incubating with Duolink blocking solution according to the manufacturer’s indications (Duolink® in situ PLA kit, Sigma). Cells were incubated with rabbit polyclonal anti-HA.11 and mouse monoclonal IgG1k anti-GFP antibodies at 4°C overnight ...
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bioRxiv - Immunology 2020Quote: ... Ear homogenate supernatants were assayed for chemokines and cytokines with the Milliplex MAP Mouse Cytokine/Chemokine Magnetic Bead Panel kit (Millipore). Samples were collected and analyzed using BioPlex 200 reader (BioRad).
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bioRxiv - Microbiology 2021Quote: Protease assays were performed with a protease fluorescent detection kit using casein labeled with fluorescein isothiocyanate (FITC) as the substrate following the manufacturer’s instructions (PF0100, Sigma-Aldrich). Briefly ...
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bioRxiv - Immunology 2021Quote: ... and PBS-treated (n ≥ 3) control human gut xenografts and mouse jejunum were purified using GenElute Mammalian Total RNA kit (Sigma) following manufacturer’s instructions ...