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Citations for Millipore Sigma :
5051 - 5100 of 10000+ citations for Recombinant Human CYCS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... proteins were transferred to a hydrophilic PVDF membrane (Imobilon E, Millipore-Sigma). Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000 ...
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bioRxiv - Immunology 2023Quote: ... proteins were transferred to a hydrophilic PVDF membrane (Imobilon E, Millipore-Sigma). Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Protein concentration was determined using the bicinchoninic acid assay kit (Sigma-Aldrich). Prior to their use ...
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bioRxiv - Systems Biology 2023Quote: ... We then transferred proteins to a low background PVDF membrane (Millipore, IPFL00010) for 15h at 15V using the TRIS-Glycine buffer (48 mM Tris ...
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bioRxiv - Microbiology 2022Quote: ... proteins were electrotransferred to a polyvinylidene difluoride membrane (Millipore, Burlington, MA, USA) using a semi-dry transfer blotter (Atto) ...
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bioRxiv - Cell Biology 2023Quote: ... Bound proteins were eluted in Flag peptide (Sigma, F4799, 150 ng/μl) for 30 minutes ...
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bioRxiv - Biophysics 2023Quote: Pure proteins were concentrated using 10 kDa Amicon Ultra membrane concentrators (Millipore), flash-frozen in liquid nitrogen and stored at −80℃.
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using the Bicinchoninic acid assay (BCA, Sigma-Aldrich). Proteins were then denaturated with Laemmli Buffer (Tris pH 6.8 (200mM) ...
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bioRxiv - Neuroscience 2023Quote: ... transferrin and transthyretin) using the ProteoPrep 20 plasma protein immunodepletion kit (Sigma), by performing two immunodepletion cycles ...
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bioRxiv - Biochemistry 2023Quote: ... Both the proteins were concentrated in a 50,000 MWCO Amicon Concentrator (Millipore) to <1 mL and injected onto a Superdex™ 200 10/300 GL Increase size-exclusion column (GE Healthcare ...
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bioRxiv - Cancer Biology 2023Quote: ... total protein was visualized using PonceauS staining (Sigma-Aldrich, St. Louis, MO), blocked using 5% non-fat milk powder in tris-buffered saline with tween (TBST ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted protein was then concentrated using 3K-50K MWCO Amicon concentrators (Millipore). All proteins were aliquoted in PCR tubes ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted protein was then concentrated using 3K-50K MWCO Amicon concentrators (Millipore). All proteins were aliquoted in PCR tubes ...
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bioRxiv - Bioengineering 2023Quote: ... mouse monoclonal anti-glial fibrillary acidic protein (1:1000, Millipore Sigma, #MAB3402), rat monoclonal anti-CD45 (1:200 ...
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bioRxiv - Microbiology 2023Quote: ... 50 µg protein per sample were reduced with 10 mM DTT (Sigma) for 30 min ...
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bioRxiv - Plant Biology 2023Quote: ... the proteins were transferred to a polyvinylidene fluoride membrane (Millipore, Billerica, USA), which was then blocked with 5% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were precipitated with 1:4 trichloroacetic acid solution (TCA, Sigma Aldrich) and washed with acetone ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were to the following proteins: Rat anti-MBP (Millipore, MAB386), Rabbit anti-SMI32 (Abcam ...
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bioRxiv - Cancer Biology 2024Quote: mTOR kinase domain protein was purchased from Sigma-Aldrich (Cat # 14-770). ETV7 protein (Cat # TP307742 ...
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bioRxiv - Physiology 2024Quote: ... Proteins were then transferred to PVDF membranes (Millipore, Burlington, MA, USA, #IPFL00010) in NuPAGE transfer buffer at 20V for 60 minutes at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were then transferred onto Immobilon-P PVDF membranes (#IPVH00010; Millipore) for immunoblotting ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were visualized using Immobilon Western Chemiluminescent HRP Substrate (Millipore Sigma, WBKLS0500) on Pro Signal Blotting film (Genesee Scientific ...
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bioRxiv - Genomics 2023Quote: ... Proteins were visualised using Immobilon Forte HRP substrate (Merck Millipore, MA, USA) and a Fusion FX Chemi Imager (Vilber Lourmat ...
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bioRxiv - Plant Biology 2024Quote: ... MAPK6 and MKP2 protein were detected with anti-AtMPK3 antibody (Sigma M8318), anti-AtMPK6 antibody (Sigma M7104 ...
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bioRxiv - Microbiology 2023Quote: ... Following a reduction of proteins with 10 mM DTT (Sigma-Aldrich, Germany) in 100 mM TEAB at 57 °C for 30 min and dehydration of gel pieces ...
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bioRxiv - Molecular Biology 2023Quote: ... Transferred proteins were stained in Ponceau S (0.1% Ponceau S (Sigma-Aldrich), 5% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... The elution containing native DnaN protein was concentrated by ultrafiltration (Millipore, UFC901096) and maintained in 20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: Cells for protein extraction were lysed using RIPA lyses buffer (Sigma #R0278) containing protease and phosphatase inhibitor cocktails (cOmplete™ ...
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bioRxiv - Microbiology 2023Quote: ... Eluted protein was concentrated using spin concentrators (Millipore Amicon Ultra-15 -3K) to ∼1.5-2 mL and subsequently dialyzed in one liter of storage buffer (150 mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... protein synthesis was inhibited by blocking translation step using cycloheximide (Sigma, #C7698) and MMP activity was inhibited by ilomastat (GM6001) ...
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bioRxiv - Molecular Biology 2023Quote: ... and protein was extracted in RIPA buffer containing protease inhibitor mixture (Sigma) and phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti-glial fibrillary acidic protein (GFAP, 1:1000, Millipore Sigma, USA), anti-NeuN (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies to the following proteins were used in this study: 53BP1 (Millipore), γ-H2AX (Ser 139 ...
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bioRxiv - Biochemistry 2023Quote: ... Gels were stained with ReadyBlue Protein Gel Stain from Sigma-Aldrich (USA) and imaged and analyzed on a ChemiDoc Imaging System by Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2023Quote: Total protein was extracted from cell lines using TRI Reagent (Sigma, UK). 40μg of isolated protein samples were then separated using 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Physiology 2023Quote: ... Final protein concentrations were calculated by Bradford assay (Sigma-Aldrich Canada, Ltd.,) according to manufacturer’s guidelines with bovine serum albumin (BioRad Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... total protein was detected with Amido Black (Sigma-Aldrich, St. Louis, MO). Chemiluminescence was measured in a ChemiDoc Imaging System (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... Proteins were visualized using peroxidase-coupled secondary antibodies (rabbit-POD, Sigma-Aldrich, A6154 ...
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bioRxiv - Cancer Biology 2023Quote: ... for 2 h on ice before mixing with protein G-Sepharose (Millipore). The antibody complex was washed three times in lysis buffer and once in Tris-buffered saline (TBS) ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 µl Magna ChIP™ Protein A Magnetic Beads (Sigma, 16-661) and antibodies (1 µg per 1 million-cell chromatin ...
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bioRxiv - Genetics 2023Quote: ... Protein lysate was prepared in Radio-immunoprecipitation assay (RIPA) buffer (Merck Millipore) with a protease inhibitor cocktail (Roche ...
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bioRxiv - Bioengineering 2024Quote: ... Protein expression was then induced by addition of 1M IPTG (Sigma-Aldrich), before harvesting of cells after 8 hours ...
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bioRxiv - Cell Biology 2023Quote: ... and protein analysis of tau and β-III-tubulin (Sigma T-8660) by western blot as described above.
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bioRxiv - Biochemistry 2023Quote: ... Proteins were then transferred to 0.45 µm PVDF membranes (Immobilon-P, Millipore) in a wet tank apparatus ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins were transferred to polyvinylidene fluoride (PVDF) membrane (Millipore, Cat# ISEQ00010), and blocked with blocking buffer (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Protein was labeled using an atto-488-NHS ester (Sigma Aldrich 41698) and then dialyzed again to remove unconjugated dye ...
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bioRxiv - Immunology 2023Quote: ... proteins (50 μM) and TCEP (5 mM) reducing agent (Sigma Aldrich, USA) were mixed 1 hour at RT and then left overnight (O/N ...
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bioRxiv - Microbiology 2024Quote: ... The cleaved protein was concentrated using Amicon 10 KDa MWCO concentrators (Millipore), and further purified by size exclusion chromatography using a Superdex 200 (16/60 ...
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bioRxiv - Microbiology 2024Quote: ... the separated proteins were transferred onto Immobilon-P PVDF membrane (Merck Millipore). The membrane was blocked with 10% skim milk in Tris Buffered Saline ...
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bioRxiv - Immunology 2023Quote: Dry protein pellets were dissolved in 100mM TrisHCL (pH 8.5, Sigma T1503) to determine the protein concentration using the Bradford protein assay (Thermo Scientific 23200) ...