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Citations for Millipore Sigma :
4951 - 5000 of 10000+ citations for Recombinant Human CYCS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The cleaved protein was concentrated using Amicon 10 KDa MWCO concentrators (Millipore), and further purified by size exclusion chromatography using a Superdex 200 (16/60 ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were then transferred onto Immobilon-P PVDF membranes (#IPVH00010; Millipore) for immunoblotting ...
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bioRxiv - Cancer Biology 2024Quote: mTOR kinase domain protein was purchased from Sigma-Aldrich (Cat # 14-770). ETV7 protein (Cat # TP307742 ...
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bioRxiv - Physiology 2024Quote: ... Proteins were then transferred to PVDF membranes (Millipore, Burlington, MA, USA, #IPFL00010) in NuPAGE transfer buffer at 20V for 60 minutes at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... MAPK6 and MKP2 protein were detected with anti-AtMPK3 antibody (Sigma M8318), anti-AtMPK6 antibody (Sigma M7104 ...
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bioRxiv - Microbiology 2024Quote: ... Optimal protein expression and solubility was achieved in RosettaTM(DE3) pLysS (Novagen) for wild-type His::PfPKG ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were then transferred to 3 kDa protein-filter columns (Sigma-Aldrich) and centrifuged at 12,000g for 45 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... Rabbit polyclonal antibody against protein disulfide isomerase (1:100) (PDI; P7122, Sigma). Secondary antibody Alexa Fluor 555 (A-21422 ...
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bioRxiv - Neuroscience 2024Quote: ... Mouse Anti-Glial Fibrillary Acidic Protein (1:100, Millipore, Cat# MAB360, RRID:AB_11212597), Mouse Anti-NeuN (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-amyloid precursor protein (APP, C-terminal, 1:1000, Sigma, #A8717), rat anti-green fluorescent protein (GFP ...
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bioRxiv - Immunology 2024Quote: ... Protein extracts were immunoprecipitated with a PP2A C-specific antibody (1D6; Millipore). An appropriate phosphorylated peptide (amino acid sequence K-R-pT-I-R-R ...
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bioRxiv - Microbiology 2024Quote: ... The procedure involved reducing the proteins with 10 mM DTT (Sigma-Aldrich), followed by carbamidomethylation with 100 mM iodocetamide (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... Protein bands were detected by Luminol-based enhanced chemiluminescence (EMD Millipore WBKLS0500). Immunoblots were captured on film and quantified using Image J.
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bioRxiv - Biophysics 2024Quote: ... BCA Protein Assay Kit and oligonucleotides were purchased from Sigma Aldrich (international). Low melting temperature agarose was purchased from Fisher Bioreagents (Massachusetts ...
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bioRxiv - Cell Biology 2023Quote: ... we precipitated the eluted proteins by adding trichloroacetic acid (#T0699, Sigma Aldrich) to 20% final concentration ...
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bioRxiv - Molecular Biology 2023Quote: ... Bead-bound proteins were eluted with 200 ng/µL 3ξFLAG peptide (Sigma) by incubating at 4°C for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... for FLAG immunoprecipitation or Protein A agarose beads (EMD Millipore 16-125) for LONP1 immunoprecipitation on a rotating platform at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... and protein was visualized using Immunobilon Western Chemilumiescent HRP Substrate (Millipore, WBKLS0500) on an ImageQuant LAS 4000 (GE Healthcare) ...
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bioRxiv - Microbiology 2024Quote: ... RNA and proteins were extracted from the fractions using Trizol reagent (Sigma) as per manufacturer’s instruction and the RNA from the polysomal fractions (≥3 associated ribosomes ...
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bioRxiv - Cell Biology 2023Quote: ... DNA/protein complexes were then precipitated with anti-CTCF antibody (EMD Millipore) or rabbit IgG conjugated with protein A and G beads ...
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bioRxiv - Genetics 2024Quote: ... Captured protein was detected by immunoblotting with anti-FLAG M2 antibody (Sigma) and visualized using an Odyssey Infrared Imaging System (Licor) ...
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bioRxiv - Biochemistry 2024Quote: ... immunoreactive proteins were visualized using Immobilon® Classico Western HRP Substrate (Millipore) or Immobilon® Forte Western HRP Substrate (Millipore ...
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bioRxiv - Bioengineering 2023Quote: ... The proteins were transferred to polyvinylidene fluoride (PVDF) membrane (Millipore, Cat# ISEQ00010), and blocked with blocking buffer (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... The pooled protein was concentrated using a centrifugal concentrator (Millipore, 100 kDa) prior to injection onto a Superdex 200 Increase 10/300 GL (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... A mixture consisting of rCas9NLS protein (0.66 mg/ml, Sigma-Aldrich CAS9PL), Phenol red (0.05% ...
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bioRxiv - Bioengineering 2024Quote: ... One hundred µL of protein samples or lysostaphin standards (SIGMA-L9043-5MG) diluted in 0.01% Tween 80 (SIGMA-P5188-100ML ...
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bioRxiv - Cancer Biology 2024Quote: ... equal amounts of protein were run and transferred onto nitrocellulose membranes (Millipore). The membranes were blocked with 5 % milk and incubated with the following antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined by BCA assay (Sigma Aldrich, cat no: B9643). 30-40 μg protein was loaded in Mini-PROTEAN® Tetra Cell Systems and TGX™ precast gels ...
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bioRxiv - Cell Biology 2024Quote: ... resuspended cells were supplemented with Bug Buster protein reagent (Millipore #70584-3) before being sonicated ...
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bioRxiv - Cancer Biology 2024Quote: ... as per manufacturer instructions and 0-5µg/mL OVA protein (Millipore Sigma) for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... The separated proteins were transferred to a PVDF membrane (Millipore, 0.2 μm). The membrane was blocked in 5% nonfat dry milk (Biofroxx ...
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bioRxiv - Molecular Biology 2024Quote: ... Separated proteins were transferred to Immobilon FL polyvinylidene difluoride (PVDF) membrane (Millipore) for immunoblotting ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins were transferred to polyvinylidene fluoride (PVDF) membrane (Millipore, Cat# ISEQ00010), and blocked with blocking buffer (Bio-Rad ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins were electrotransferred to Immobilon-FL polyvinylidene difluoride (PVDF) membranes (Merck Millipore) and blocked with Intercept Blocking Buffer (LI-COR Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... Protein was labeled using an atto-488-NHS ester (Sigma Aldrich 41698) and then dialyzed again to remove unconjugated dye ...
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bioRxiv - Bioengineering 2024Quote: ... Protein expression was then induced by addition of 1M IPTG (Sigma-Aldrich), before harvesting of cells after 8 hours ...
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bioRxiv - Immunology 2023Quote: ... H3K27me3 or HK3K27Ac ChIP-grade antibodies and Protein A magnetic beads (Millipore). FAIRE was performed on samples fixed and sonicated as per ChIP ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested overnight at 37C with 1:50 trypsin (Sigma-Aldrich) and 1:75 and lysyl endopeptidase (Wako) ...
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bioRxiv - Genetics 2023Quote: ... Proteins were transferred to a PVDF membrane (Immobilon-FL, Millipore, Burlington, MA) and blocked for 1 h at room temperature in Odyssey blocking buffer (Li-Cor ...
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bioRxiv - Biochemistry 2023Quote: ... Antibody precipitated with 100 µL Protein A/G beads (Millipore, IP05-1.5ML) was washed twice in 700 µL DRIP buffer ...
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bioRxiv - Microbiology 2023Quote: ... followed by the transfer of protein onto the PVDF membrane (Merck Millipore) using a wet-tank blotting system (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were then transferred to PVDF membrane (Immobilon, Millipore, Billerica, MA, USA) by semi-dry blotting ...
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bioRxiv - Neuroscience 2023Quote: ... the proteins were reduced with 10mM tris(2-carboxyethyl)-phosphine (TCEP) (Sigma) for 20 min ...
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bioRxiv - Cell Biology 2023Quote: ... Transferred proteins were then stained with Ponceau S Solution (Sigma-Aldrich P3504). Blots were blocked (5% (w/v ...
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bioRxiv - Physiology 2023Quote: ... Proteins were detected based on HRP substrate-based enhanced chemiluminescence (#WBKLS0500, Millipore), visualized using ChemiDoc Touch Imaging System (BioRad ...
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bioRxiv - Biochemistry 2022Quote: ... then stained with ReadyBlue™ Protein Gel Stain from Sigma-Aldrich (USA) and imaged and analyzed.
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bioRxiv - Molecular Biology 2022Quote: Total proteins were extracted from cells using RIPA buffer (Sigma-Aldrich, #R0278) and quantified using the Pierce BCA Protein Assay Kit (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Antibodies used for probing for specific proteins were: actin (Sigma A-2066), Hsp70 (Thermo-Fisher MA 3006) ...
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bioRxiv - Cell Biology 2023Quote: ... Rab proteins were either loaded with GDP (REP-complexes, Sigma Aldrich, Germany) or MANT-GDP (GDI-complexes ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were then transferred to polyvinylidene difluoride membranes (EMD Millipore, Darmstadt, Germany), blocked with 5% dry milk in TBS ...