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Citations for Millipore Sigma :
5001 - 5050 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... were seeded in 6-well plates (1.5×106 cells/well) with or without phorbol 12-myristate 13-acetate (PMA, 100 ng/ml, 2 ml/well, Sigma-Aldrich) and incubated at 37°C for 48 hrs ...
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bioRxiv - Microbiology 2024Quote: ... and transferred to individual wells of a 24-well plate cell culture plate in water with 200 µM phenylthiourea (PTU, Sigma-Aldrich) and stored in an incubator at 28 C.
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bioRxiv - Neuroscience 2024Quote: ... on PEI/Laminin-coated surfaces at 55,000-60,000 cells/cm2 (200k/well of a 12 well) in N2/B27 medium supplemented with 1 mg/mL doxycycline (Sigma-Aldrich), 10 ng/mL BDNF (R&D systems) ...
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bioRxiv - Immunology 2020Quote: ... Cell supernatants were harvested after 96 hours and filtered through 0.45-μM filters (Millipore). Antibodies were purified via HiTrap Protein A HP columns (GE Healthcare ...
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bioRxiv - Physiology 2023Quote: ... using a Superose 6 Increase 10/300 GL column (Sigma-Aldrich, # GE29-0915-96) equilibrated in 1X PBS ...
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bioRxiv - Cell Biology 2019Quote: ... glass-bottom dishes (IWAKI, Shizuoka, Japan) were treated with 2% acetic acid (WAKO) and 0.2% 3-(Trimethoxysilyl)propyl methacrylate (SIGMA) in 80% ethanol for 2 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Meiotic cell cultures (∼150μl) were placed on bottom-glass dishes (Matsunami) pre-coated with Concavalin A (10 mg/ml, Sigma).
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bioRxiv - Cell Biology 2020Quote: ... fluorescently tagged protein were seeded in 3.5 cm glass bottom dishes (FD35–100 for 24 hr in media containing 10 μM bromodeoxyuridine (BrdU–Sigma) and 0.5–1 μg/ml tetracycline hydrochloride (Sigma) ...
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bioRxiv - Biochemistry 2022Quote: ... USA) were placed in a three-neck round-bottom flask in the presence of oleic acid (Sigma-Aldrich, USA), oleylamine (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2019Quote: ... protoplasts were transferred to 35 mm glass bottom MatTek dishes (coverslip thickness #1.5) coated with poly-L-lysine (Sigma) and imaged ...
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bioRxiv - Genetics 2020Quote: ... The cauda epididymis was nicked three times to allow sperm to swim out and placed in a 2ml round bottom Eppendorf tube filled with 1.1ml of 37°C Human Tubal Fluid media (Millipore). Sperm were placed in a 37°C incubation chamber and allowed to swim out for 10 minutes before the cauda epididymis was removed ...
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bioRxiv - Bioengineering 2021Quote: ... aggregates were re-plated on Glass Bottom Cell Culture Dish (Nest) previously coated with poly-L-ornithine (15μg/mL, Sigma) and laminin (20μg/mL ...
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bioRxiv - Developmental Biology 2021Quote: ... Clusters were then plated into glass-bottom dishes coated with 62.5 μg ml−1 of fibronectin (Sigma-Aldrich, F1141) and let to adhere to the substrate for 15–30 min ...
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bioRxiv - Cell Biology 2021Quote: ... NCMs and NCFs were removed from cell suspension by centrifugation 30 min at 3000 rpm in a discontinuous Percoll gradient (bottom 58.5%, top 40.5%, Sigma-Aldrich).
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bioRxiv - Microbiology 2022Quote: ... the bottom channel of a polydimethylsiloxane (PDMS) device was precoated with fibronectin (3 μg/ml, Sigma-Aldrich, Cat# F1141). The microfluidic device was generated according to our previous report70 ...
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bioRxiv - Cell Biology 2024Quote: ... embryos were mounted on a 35-mm glass-bottom dish using 100-200 μL of 0.5% low-gelling temperature agarose (Sigma) in PBS prewarmed to 40°C ...
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bioRxiv - Cell Biology 2023Quote: ... the gels were transferred to a 6 cm glass bottom dish pre-coated with 0.1% (weight/volume) Poly-D-lysine (Sigma) for imaging.
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bioRxiv - Microbiology 2023Quote: ... the bottom channel of a polydimethylsiloxane (PDMS) device was precoated with fibronectin (3 μg/ml, Sigma-Aldrich, Cat# F1141). The microfluidic device was generated according to our previous report65 ...
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bioRxiv - Developmental Biology 2023Quote: ... ganglionic eminences explants were cultured on glass bottom petri dishes previously coated with poly-L-Lysin (2mg/ml, Sigma), fibronectin (5µg/cm 2 ...
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bioRxiv - Microbiology 2022Quote: ... the bottom channel of a polydimethylsiloxane (PDMS) device was precoated with fibronectin (3 μg/ml, Sigma-Aldrich, Cat# F1141). The microfluidic device was generated according to our previous report63 ...
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bioRxiv - Cancer Biology 2023Quote: ... zebrafish embryos were placed on glass-bottom petri dishes and covered with 1% low melting agarose containing 0.003% tricaine (Sigma). Images were acquired using a Leica SP8 confocal microscope (Leica ...
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bioRxiv - Developmental Biology 2023Quote: ... and explants were plated on glass coverslips or ibidi 35mm glass bottom dish coated with 100 μg·mL-1 poly-lysine and 20 μg·mL-1 Laminin (Sigma Aldrich). Cells were cultured for 24 hours in culture medium supplemented with 0.5% (w/v ...
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Impact of light pollution at night on male reproductive success in Japanese medaka (Oryzias latipes)bioRxiv - Zoology 2023Quote: ... The males were distinguished by randomly clipping either the top or bottom corner of the tail fin (supplemental figure 1A) under anesthesia with 0.03% Tricaine methanesulfonate (MS222, Sigma). The behavior of the fish in triads was observed for 1-3 minutes each at three time points in the morning for 5-8 days ...
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bioRxiv - Physiology 2023Quote: ... The cells were seeded in 35 mm glass-bottom dishes (Mat-Tek) pre-coated with Poly-L-lysine (Sigma) and transfected with the different constructs one day before imaging ...
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bioRxiv - Molecular Biology 2021Quote: ... 7 x105 HEK293 cells were seeded in each well of a 6-well plate and were transfected with 2 μg of respective plasmid DNA using PEI reagent (Sigma-Aldrich, 408727). After 24 hours ...
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bioRxiv - Biochemistry 2022Quote: ... HEK-293 cells transfected with either V2RWT or V2RT360A were seeded at a density of 0.2 million per well in a 24-well plate precoated with 0.01% poly-D-Lysine (Sigma-Aldrich; cat. no. P0899). After 24hr ...
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bioRxiv - Neuroscience 2021Quote: ... WT DRG neurons were plated in 20 μg/ml PLL + 5 μg/ml Laminin coated 24 well plates at a density of 5000 cells per well in complete medium (DMEM/F12 (Sigma-Aldrich, D8437) supplemented with 1× B27 (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: ... Isolated primary microglia were seeded at 500,000 cells per well (24 wells plate) and treated one day after with 2 μg/ml of Lipopolysaccharide (LPS, Sigma, #L3024-5MG) for 1h ...
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bioRxiv - Genomics 2019Quote: Cells were seeded at a density of 4 × 104 cells per well in laminin coated 4 well chamber slides (Millicell® EZ slide, Merck Millipore, PEZGS0816). Cells were washed in cold PBS and fixed using 4% paraformaldehyde (PFA) ...
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bioRxiv - Microbiology 2021Quote: ... Wells were washed again and probed with 100 μl/well of a α-mouse alkaline phosphatase-conjugated secondary antibody (Sigma Aldrich, A4187) diluted 1:2,000 and incubated for one hour at room temperature ...
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bioRxiv - Systems Biology 2020Quote: ... we split and seeded iPS-GM942-SeV3 into Geltrex-coated 12-well plates at a density of 3 × 105 cells per well in iPS-Brew + 2μM Thiazovivin (Sigma, cat. SML1045-5MG). After 24 hours ...
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bioRxiv - Immunology 2021Quote: ... BMDC clusters were harvested on day 8-10 in 5 mls of comlete I-10 media at room temperature and then stimulated at 1-2x106 cells per well of an untreated 6-well plate (Sigma M9062-100EA) in 2 mls of I-10 medium without GMCSF.
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bioRxiv - Cell Biology 2019Quote: ... 15,000 THP1 cells were seeded per well in 384 well plate and were transfected with either universal negative control 1 (UNC1) (Millipore Sigma, SIC001) or esiRNA human TFEB (Millipore Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... 1×105 HEK 293T cells were re-suspended in 0.5 mL of complete media and seeded into each well of a 12-well dish (Millipore Sigma Cat. CLS3513). On the next day ...
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bioRxiv - Immunology 2024Quote: ... The minced tumor tissue was transferred to a 6-well plate containing 3 mL/well of tumor digestion media (NK cell media + 1 mg/mL collagenase IV (Sigma, cat. C5138) + 0.2 mg/mL DNAse I (Roche ...
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bioRxiv - Immunology 2023Quote: ACE2-HEK293 ΔRIG-I cells were seeded at 5×10E4 cells per well in 24-well plates that had been treated with Poly-D-lysine hydrobromide (Sigma, P6407-5MG) and reverse transfected with 50 nM/well of control (CTRL ...
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bioRxiv - Genomics 2024Quote: ... neural crest cells were passaged onto fibronectin-coated 6-well plates (about 1 million cells per well) using Accutase (Sigma-Aldrich A6964) and fed with neural crest maintenance medium (1:1 ratio of DMEM-F12 and neurobasal ...
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bioRxiv - Plant Biology 2020Quote: ... water was replaced with a solution containing 30mg/L (w/v) luminol (Sigma-Aldrich) and 20mg/L (w/v ...
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bioRxiv - Plant Biology 2019Quote: ... After adding 110 µl of 40% PEG solution [40% (w/v) PEG-4000 (Sigma), 0.8 M mannitol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 30ml Nipagin 10% w/v) spread with yeast paste (Saccharomyces cerevisiae – Sigma-Aldrich #YSC2). Eggs were removed from the agar plate with phosphate buffered saline (PBS ...
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bioRxiv - Molecular Biology 2019Quote: ... Coverslips were first coated with 0.1% (w/v) poly-L-lysine (Sigma-Aldrich, #P8920) for 15 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... The spheroid-forming medium contained 0.24% (w/v) methyl cellulose (Sigma, Cat No. M0512). The lid was then turned upside down and placed on top of the plate filled with 10 mL sterile water ...
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bioRxiv - Developmental Biology 2021Quote: ... prefilled halfway with solidified 1.0% agarose (1.0% agar w/v, planarian water) (Sigma A9539). The remaining petri dish volume was filled with 1.0% agarose until the agarose level is flush with the top of the encapsulated planarian ...
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bioRxiv - Bioengineering 2020Quote: ... a few drops of 0.1% (w/v) L-ascorbic acid (Cat# A7631, Sigma-Aldrich) were placed on a separate filter ...
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bioRxiv - Developmental Biology 2021Quote: ... Animals of genotype oyIs44 V were mutagenized using 70 mM ethyl methanesulfonate (EMS, Sigma) at 20°C for 4 hours ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were seeded onto plates coated with 0.1% (w/v) Gelatin (Sigma-Aldrich, G1890) in PBS for routine culture and plates coated with 5 ng/mL Laminin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... brain slices were placed in 1 × PBS containing 4% (w/v) acrylamide (Sigma-Aldrich) and 0.25% (w/v ...
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bioRxiv - Biophysics 2022Quote: ... Poly-L-lysine solution 0.1 % (w/v) in water was purchased from Sigma-Aldrich. GFP plasmid (pCMV-GFP) ...
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bioRxiv - Biophysics 2022Quote: ... 150 μL of 5% (w/v) polyvinyl-alcohol with molecular weight of 145,000 (Millipore) were spin-coated on plasma-cleaned coverslips ...
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bioRxiv - Microbiology 2020Quote: ... Non-specific binding was blocked using 5% (w/v) bovine serum albumin (BSA; Sigma) in Tris-buffered saline (pH 7.4 ...