Labshake search
Citations for Millipore Sigma :
4951 - 5000 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... sorted LSK cells were seeded in 48 well plates (6000 cells/well) in Stemline II haematopoietic stem cell expansion medium (Sigma, S0192) containing 50 ng/ml SCF (Peprotech ...
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bioRxiv - Neuroscience 2021Quote: ... Each well of 6-well cell culture treated plates coated with 0.1% Poly-L-ornithine (3 ug/mL; Sigma Aldrich, P4957) were seeded with ∼750K cells and maintained with sterile-filtered neurobasal medium (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... 2D viability control cultures were seeded in 12-well cell culture plates on wells coated with poly-D-lysine (Sigma-Aldrich) at a density of 288,000 cells/well.
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bioRxiv - Immunology 2022Quote: ... cells were seeded at a density of 1 × 106 cells per well in 12-well plates and stimulated with LPS (100 ng/mL, M1-like - Sigma-Aldrich), IL4 (10 ng/mL ...
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bioRxiv - Immunology 2021Quote: 6.125×106 THP1 cells were seeded per well in 6-well plates in growth medium containing 10 ng/ml PMA (Sigma-Aldrich). As a control ...
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bioRxiv - Cell Biology 2021Quote: ... cells were plated on cell culture plates (48-well or 24-well) coated with 0.01 % Poly-L-Ornithine (Sigma-Aldrich, USA) for 1 h at RT and 20 μg/mL Laminin (Roche ...
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bioRxiv - Neuroscience 2020Quote: ... Electroporated PBMCs were transferred into 1 well of a 6 well plate seeded with MEFs in PBMC recovery medium (RPMI supplemented with 200uM 1-thioglycerol (Sigma M6145), 1uM Dexamethasone (Sigma D1756) ...
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bioRxiv - Microbiology 2020Quote: ... cells were plated on 24-well plates at 3×105 cells/well with 0.1 µg/mL 12-O-tetradecanoylphorbol-13-acetate (TPA, Sigma-Aldrich #P1585) to promote differentiation ...
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bioRxiv - Microbiology 2021Quote: ... Wells were washed again and probed with 100 μl/well of a α-goat alkaline phosphatase conjugated antibody (Sigma Aldrich, A4187) diluted 1:2,000 and incubated for one hour at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Human melanocytes seeded at 1.0×105 – 2.0×105 cells/well density in 6-well plates were incubated in viral supernatant with 10µg/mL polybrene (Sigma, TR-1003) and centrifuged at 300xg for 60 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... expressing a stable CCNB1-GFP construct were plated into 384 well plates with 500 cells per well and reverse transfected with an esiRNA library (10ng, Sigma Aldrich) using Hiperfect transfection reagent ...
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bioRxiv - Molecular Biology 2021Quote: ... VICs were seeded at 1×105 cells/well into 6-well culture plates and stimulated with FeSO4 (10 μmol/L) concentrated with iron(II) sulfate heptahydrate (Sigma, USA) in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 ml of this cell suspension was added per well to a 24-well ultra-low attachment plate (Sigma CLS3473). To test the effect of blebbistatin on aggregate morphology ...
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bioRxiv - Neuroscience 2022Quote: 90,000 human NPCs were plated in a well of Chambered Coverglass wells that had been coated with Poly-L-Ornithine (Sigma Aldrich) and Laminin (Sigma Aldrich) ...
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bioRxiv - Genomics 2020Quote: ... The lentivirus containing the pLenti-FNLS-P2A-Puro or pLenti-ABERA-P2A-Puro or desired gRNAs were added to the 0.5 million of HUDEP-2 or HUDEP-2 stable cell line in a one well of the six-well plate along with 6 μg/ml Polybrene (Sigma-Aldrich) and 1% HEPES 1M buffer (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... Virus-containing supernatants were collected 48 h later and added with Polybrene to AML cells pre-seeded at ∼5 × 105/well in 24-well plates (Sigma-Aldrich). After 8 h ...
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bioRxiv - Physiology 2021Quote: ... elegans were transferred into 24 well plates containing 1 mL M9 Buffer in quadruplicates for each strain and condition (three wells with sodium arsenite (Sigma-Aldrich) and one well M9 as control) ...
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bioRxiv - Cell Biology 2022Quote: ... trypsinized cells were resuspended in standard growth medium without antibiotics and plated in 6-well culture plates (5 × 104 cells/well) after mixing with 10 µg/ml polybrene (EMD Millipore) and lentivirus supernatant ...
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bioRxiv - Genetics 2022Quote: 750k low-passage 293FT cells were seeded into each well of a 6-well plate coated with poly-L-ornithine (Sigma, P2533). One day after seeding ...
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bioRxiv - Microbiology 2019Quote: ... innocua cultures or the LM cocktail were dispensed into individual wells in 12 well plates (Corning-Costar, CLS3512, supplied by Sigma-Aldrich). The plates were exposed to UV-C light for 20 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... were added to each well (around 6 × 105 cells per well) along with a no virus control with 8 µg/mL polybrene (Sigma-Aldrich). Cells were incubated overnight (12-16 hours) ...
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bioRxiv - Molecular Biology 2020Quote: For lentiviral transduction 200.000-250.000 ES cells per well were seeded on gelatinized 6-well dishes in ES medium containing 8 μg/ml polybrene (Sigma, #H9268). Viral supernatant was added to the cells and plates were spun in a prewarmed centrifuge (Heraeus Multifuge 4KR ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were plated overnight at 1 × 106 cells/well in six-well plates coated with poly-L-lysine (Sigma-Aldrich) and transfected the following day using PEI reagent according to the published optimized procedures69 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were passaged to single cell suspension and plated to achieve 65,000 cells per well of a 6-well plate in Nutristem supplemented with 1:2,000 Y-27632 (ROCK inhibitor; Sigma Aldrich, Y0503) [23] ...
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bioRxiv - Physiology 2020Quote: ... and seeded onto 6-well plates (105 cells/well) in osteogenic medium (50μg/mL L-ascorbic acid-2-phosphate and 5mM β-glycerophosphate) (Sigma, UK) for 24 days ...
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bioRxiv - Immunology 2019Quote: ... cells (1×106/well in a 24-well plate) were cultured for 4 h at 37°C with Ionomycin (10 ng/ml, Sigma-Aldrich), Brefeldin (5 mg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... Adherent bone marrow stromal cells were then re-plated in triplicate in 12 well plates at 2×105 cells per well with ascorbic acid and 10 mM β‐glycerophosphate (Sigma) to perform qPCR assays ...
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bioRxiv - Cancer Biology 2021Quote: ... each well of 6 well-plate were transferred to a 10cm plate with previously determined concentrations of puromycin (Sigma-Aldrich, USA), for that cell line for 3 days ...
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bioRxiv - Neuroscience 2021Quote: ... plated at a density of 2-5 × 104 cells /well on coverslips in a 24-well plate pre-coated with poly-L-lysine (Sigma, P9155) and cultured in a Neurobasal medium supplemented with 2% B27 supplement ...
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bioRxiv - Molecular Biology 2020Quote: ... a single adult male or female worm was cultured in one well of a standard 24-well cell culture plate (Sigma-Aldrich). For infective L3 stage worms ...
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bioRxiv - Molecular Biology 2021Quote: ... These parasites were cultured (10 sex-separated adults or five adult pairs per well in a 48 well tissue culture plate) at 37°C in DMEM (Sigma-Aldrich) supplemented with 10% foetal calf serum ...
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bioRxiv - Microbiology 2021Quote: ... four washes were performed (200 µL of 1xPBST/well) and incubated with pNPP liquid substrate (50 µL/well) (pNPP, Sigma-Aldrich) at 37 °C for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... each piece of frozen tissue was placed into one well of a 6-well plate with salt-Tris buffer containing 146 mM NaCl (Cat#S6546-1L, Sigma-Aldrich), 1 mM CaCl2 (Cat#97062-820 ...
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bioRxiv - Microbiology 2022Quote: ... The cultures were harvested by centrifuging for 5 minutes at 12,000 rpm and resuspended in LB and transferred to wells of 12-well plate containing glass coverslips coated with polylysine (Sigma-Aldrich). The plate was incubated at 37°C for 48 hours after which the biofilm specimens were prepared for electron microscopy as described by Ochmcke et al ...
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bioRxiv - Neuroscience 2022Quote: ... 15,000 neurons were plated per well in 24-well plates on coverslips coated with poly-DL-ornithine (Sigma-Aldrich Cat#P0671) and laminin (Corning Cat#354232 ...
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bioRxiv - Cancer Biology 2022Quote: LLC cells were treated with conditioned media from inflammasome-induced macrophages as indicated and 5 × 104 cells per well were seeded in top chambers of the transwell chamber (8 μM pore, 24-well plate, Millipore-Sigma) in FBS-free media with membrane inserts ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... cells were plated on 24-well plates at 3x105 cells/well with 0.1 μg/mL 12-O-tetradecanoylphorbol-13-acetate (TPA, Sigma-Aldrich P1585) to promote differentiation ...
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bioRxiv - Cell Biology 2024Quote: ... 1×105 ESCs were placed per well of a 12-well plate coated with 16.7 μg/mL bovine plasma fibronectin (Sigma-Aldrich, F1141) in N2B27 medium containing 20 ng/mL Activin A (Peprotech ...
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bioRxiv - Neuroscience 2024Quote: ... Approximately 7 x 105 cells/well were plated onto each well of 10 µg/mL Poly-D-lysine (PDL, Sigma-Aldrich, P6407-5MG ...
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bioRxiv - Microbiology 2023Quote: ... wells were washed twice and stained with 180 μL per well of 0.1% crystal violet (Sigma-Aldrich, Castle Hill, NSW, Australia) for 15 minutes and 180 μL per well of acetic acid was added to the plates to elute the crystal violet stain ...
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bioRxiv - Physiology 2023Quote: THP-1 monocytes were differentiated into THP1-derived macrophages by stimulating 5×105 THP-1 monocytes/well in 6-well plate with 100 ng/mL phorbol 12-myristate 13-acetate (PMA) (#P8139, Sigma Aldrich) for 48 h ...
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bioRxiv - Bioengineering 2023Quote: ... 150 μl of the culture was added to each well and the wells were covered with 50 μl of mineral oil (Sigma-Aldrich) to prevent evaporation ...
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bioRxiv - Cell Biology 2023Quote: ... MPP4 (7 x 104 cells per well) were sorted directly into 8-well plates pre-coated with poly-lysine (Sigma-Aldrich) and containing XF DMEM medium ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 – 40 μg of total protein was loaded into 15-well or 12-well RunBlue 4-12% Bis-Tris gels (Westburg) with the addition of 10mM DTT (Sigma Aldrich) and 4x NuPage LDS Sample buffer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and neurons plated in NPM onto 24-well plates (5 x 104 cells/well) precoated with poly-L-lysine hydrobromide (Sigma P2636) (1 mg/mL for glass coverslips or 0.1 mg/mL for plasticware ...
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bioRxiv - Immunology 2023Quote: ... IDE12 cells were also grown to confluency in T25 flasks and either seeded at 1x106 cells/well in 6-well plates (Millipore Sigma) or 3x105 cells/well in 24-well plates (Corning) ...
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bioRxiv - Cell Biology 2023Quote: ... 20’000 transduced MEFs were then seeded into one well of a 6 well plate in ‘MES medium’ supplemented with 2ug/ml Doxycycline (D9891-5G, Sigma-Aldrich), 3μM CHIR99021 (4423/50 ...
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bioRxiv - Neuroscience 2023Quote: ... and plated at a density of 1 million cells per 6-well plate (or 375k cells per 12-well plate) in poly-L-ornithine (Sigma P3655) -coated wells in Neuron Differentiation Media with 10 uM ROCK inhibitor ...
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bioRxiv - Physiology 2024Quote: ... KD-LX2 and Scr-LX2 cells were seeded in 6-well-plates (4x104 cells/well) for 48hrs before being stained with Nile Red (Sigma-Aldrich) and evaluated with densitometry by ImageJ software to measure lipid content [24] ...
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bioRxiv - Microbiology 2023Quote: ... the media from each well was aspirated and the wells were overlaid with 2 mL/well DMEM with 5% FBS and 1.2% Avicel® PH-101 (Sigma,11363). At 7 dpi ...