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Citations for Millipore Sigma :
5001 - 5050 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... for 10 mins and incubated in blocking PBS solution containing 2% BSA (AppliChem, 9048-46-8) and 0.2% Triton™ X-100 (Sigma-Aldrich, 9002-93-1) for 30 mins to permeabilize cell membrane and block unspecific antigen binding ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibody was incubated at room temperature protected from light for 1 hour in Intercept®(TBS) Protein-Free Blocking Buffer with 0.01% Tween®20 (Sigma, St. Louis, MO) using IRDye® 800CW Goat anti-Mouse IgG secondary antibody at 1:10,000 (LI-COR Lincoln ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibody was incubated at room temperature for 2 hours in Intercept®(TBS) Protein-Free Blocking Buffer with 0.01% Tween®20 (Sigma, St. Louis, MO) using STAT1 1:1000 (#14994 ...
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bioRxiv - Cell Biology 2021Quote: ... Primary and secondary antibody incubations were performed for 1 hour at 25°C in blocking buffer with anti-Flag-M1 (Sigma-Aldrich, #F3040, 1:1000) conjugated with Alexa-647 (Molecular Probes ...
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bioRxiv - Biochemistry 2022Quote: ... The membrane is incubated for 1 h in a blocking buffer of PBST (PBS supplemented with 0.1 v/v % Tween) and 10% (v/w) dry skim milk (Sigma-Aldrich, St. Louis, MO, USA). The membrane is washed three times with PBST for 5 min and then incubated for 1 h at 25 C with a primary anti-dynein antibody (sc-13524 ...
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bioRxiv - Neuroscience 2022Quote: ... then overnight in blocking solution with the following primary antibody: 1:1000 mouse anti S-100 β subunit (S2532, Sigma-Aldrich, St. Louis, MO). Sections were washed and then incubated for 2h in blocking solution with the following secondary antibody at a 1:500 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... secondary antibodies were applied for 30 min at RT at 1:400 dilution in blocking solution containing 1:10,000 4’,6-diamidino-2-phenylindole (DAPI; Sigma Aldrich, St. Louise, MO, USA) in the closed humid chamber ...
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bioRxiv - Neuroscience 2021Quote: ... The slices were rinsed with the blocking solution and incubated with secondary antibodies for 2 h at room temperature (FITC-conjugated anti-rabbit IgG, AP132F, Millipore, Darmstadt, Germany; 1:200). The slices were then washed three times with PBS and mounted with 4’,6-diamidino-2-phenylindole (DAPI)-containing Vectashield (Vector Laboratories ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... prepared in antibody blocking solution containing 0.5% BSA (Fisher, Pittsburgh PA, USA #BP9703-100) and 0.5% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA #T9284) for 48-72 hours at 4°C in a shaker ...
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bioRxiv - Neuroscience 2019Quote: ... following by primary antibody incubation overnight at 4°C (rabbit anti-pRIPK3, Abcam; mouse anti-TH, Millipore; mouse anti-pMLKL: Millipore rabbit anti-TH, Millipore in blocking/permeabilizing solution). Sections were washed in 0.1M TBS and then incubated in secondary antibodies for 2 hours at RT in TBS (goat anti-rabbit Alexa Fluor 488 ...
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bioRxiv - Genomics 2021Quote: ... and nucleolin (D4C7O) rabbit mAb (Cell Signalling cat # 14574) appropriately diluted in Odyssey® Blocking Buffer (TBS) 0.1% TWEEN® 20 (Sigma Aldrich cat # P1379) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... in 50 μL of blocking buffer were added for 1 hour followed by 50 μL ExtrAvidin-Alkaline phosphatase (E2636, Sigma-Aldrich, 1:5000 dilution) in blocking buffer for 1 hour at room temperature (RT) ...
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bioRxiv - Neuroscience 2022Quote: ... and then blocked for 1 hour in blocking buffer (1% BSA and 10% normal donkey serum, 0.5% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA). Primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... the calcium free medium was replaced with the cell culture medium with or without E-cadherin blocking monoclonal antibody DECMA-1 (Millipore, 10 µg mL−1), and cells migration was carried out for 4 hours at 37°C in a 5% CO2 humidified incubator.
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bioRxiv - Genetics 2022Quote: ... Membranes were washed three times with TBST and incubated with blocking buffer containing Rabbit IgG HRP Linked Whole Ab (Sigma cat no. GENA934-1ML) at 1:5000 for two hours with gentle mixing ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated in primary antibodies (diluted in the blocking solution, see Table 1) against myc (rabbit c-Myc, 1:1000, Sigma-Aldrich, C3956, RRID: AB_439680) and FLAG (mouse-FLAG ...
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bioRxiv - Developmental Biology 2024Quote: ... They were then incubated overnight at 4°C with primary antibodies in blocking solution: rabbit anti-GFP (MBL Cat# 598; 1/300) and mouse anti-HA (Sigma, Cat# 12ca5; 1/50). On the next day ...
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bioRxiv - Genetics 2024Quote: ... by transferring them in a small drop of blocking solution to the surface of a 0.01% poly-L-lysine (Sigma Aldrich, Cat# 25988-63-0) coated slide ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated in blocking buffer for 1 hr at room temperature followed by incubation with rabbit anti-RBM10 (Sigma-Aldrich HPA034972, 1:2000), rabbit anti-HDAC1 (GeneTex GTX100513 ...
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bioRxiv - Biochemistry 2022Quote: ... Primary antibody were diluted in Blocking Buffer as described below and incubated with samples for 2 hours: γ-H2AX monoclonal mouse antibody JBW301 (Millipore Sigma, 1:1000). Samples were washed with Blocking Buffer twice for 5 minutes each then treated with secondary antibody diluted 1:200 for 2 hours ...
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bioRxiv - Microbiology 2023Quote: ... After cell permeabilization and blocking with 1% normal goat serum (Nacalai Tesque) in dilution buffer [0.3% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA) in PBS containing 3% bovine serum albumin (Nacalai Tesque)] ...
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bioRxiv - Cell Biology 2022Quote: ... Nitrocellulose membranes were blocked for 1 h with the blocking buffer containing 5 % dried milk in TBS-Tween 1 % and incubated over-night with primary antibodies: anti-αTubulin (Sigma, T6074, 1:1000 dilution), anti-acetylated Tubulin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... in 50 μl of blocking buffer were added for 1 h followed by 50 μl ExtrAvidin-Alkaline phosphatase (E2636, Sigma-Aldrich, 1:5.000 dilution) in blocking buffer for 1 h at RT ...
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bioRxiv - Bioengineering 2023Quote: ... iSMCs were incubated for 30 mins in blocking solution containing 2% BSA (AppliChem, 9048-46-8) and 0.5% Triton™ X-100 (Sigma-Aldrich, 9002-93-1). The blocking solution was then discarded and iSMCs were incubated for 2 hrs with primary antibodies diluted in blocking solution ...
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bioRxiv - Cell Biology 2023Quote: ... After blocking for 1 hour in blocking buffer (for Fig. 1D: 5% Protifar; for Suppl. Fig. 2B: 0.2% v/v Fish skin gelatin (Sigma–Aldrich, St. Louis, MO, USA)+ 0.1% v/v Tween–20 ...
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bioRxiv - Neuroscience 2024Quote: ... and a primary rat antibody against myelin basic protein (MBP) (1:50 in blocking solution; Cat. No. MAB386, Sigma-Aldrich, St. Louis, MO, USA). Following five 5-minute washes at 20°C with PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were subsequently washed five times with ice-cold lysis buffer freshly supplemented with 20 mM NEM and subsequently incubated with Flag peptide (2 mg/mL, Sigma-Aldrich, F-3290) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... The immunoprecipitates on protein A agarose or sepharose were either boiled directly in sample buffer or eluted with FLAG peptide (Sigma, 200 μg/mL) or HA peptide (Roche ...
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bioRxiv - Microbiology 2021Quote: ... The selected mouse and rabbit immunoglobulins were affinity purified on protein G SepFast resins (BioToolmics, UK) or on peptide used for immunization and conjugated to a cyanogen bromide-activated-Sepharose® 4B (Sigma-Aldrich, USA), respectively ...
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bioRxiv - Microbiology 2022Quote: ... were transfected with a pDisplay vector encoding amino acids 16 to 682 of SARS-CoV-2 S (S1) fused to a C-terminal hemagglutinin (HA) peptide using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). The supernatant was collected six days post-transfection ...
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Dissecting β-Cardiac Myosin and Cardiac Myosin-Binding Protein C Interactions using a Nanosurf AssaybioRxiv - Biophysics 2022Quote: ... BG-oligonucleotide-labeled SNAP proteins bound to resin were again washed with wash buffer and eluted using 0.2 mg/mL FLAG peptide (Sigma-Aldrich, St. Louis, MO) overnight at 4°C with rotation ...
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bioRxiv - Microbiology 2021Quote: ... were transfected with a pDisplay vector encoding amino acids 16 to 682 of SARS-CoV-2 S (S1) fused to a C-terminal hemagglutinin (HA) peptide using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). Supernatant was collected 6 days post-transfection ...
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bioRxiv - Biochemistry 2020Quote: ... isolated from the BBPHs were added to the reaction mix before adding the enzyme to assess the inhibition potential of the peptides and the results have been compared with a standard ACE inhibitor Lisinopril (L2777, Sigma-Aldrich, MO, USA), used as positive control (50 µL of 2 µg/mL).
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bioRxiv - Immunology 2020Quote: ... and in vitro cultured for 18-24 hours in media supplemented with or without peptide at 0.5 µM (or, as positive control, with 1 µg/ml Staphylococcal enterotoxin B (SEB, Sigma Aldrich, St. Louis, USA)) ...
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bioRxiv - Molecular Biology 2021Quote: ... Bead-particle complexes were washed once with 200 µl washing buffer (20 mM Tris-HCl pH 7.5 and 150 mM NaCl) and subsequently eluted with FLAG peptide (30 min at 37 °C; 200 μg/ml in washing buffer, Sigma Cat. No. F3290) and lysed by addition of Amphipol A8–35 (Anatrace ...
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bioRxiv - Cell Biology 2020Quote: ... As a positive control we used a bona fide Src substrate peptide corresponding to amino acids 6-20 of p34cdc2 (Millipore-Sigma, 12-140). The reactions were carried out at room temperature in a total volume of 25 μL for 40 min in white 96-well ...
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bioRxiv - Immunology 2020Quote: Following protein digestion a 5% volume of the peptide solution was cleaned up with a reverse phase (C18) ZipTip (Merck Millipore, Burlington, MA, USA). The peptide sample was acidified with the addition of 1 μl 1M hydrochloric acid ...
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bioRxiv - Cell Biology 2021Quote: The peptide/ZO1 interaction was detected in situ using the Duolink secondary antibodies and detection kit (Sigma, St Louis, MO, Cat: 92002, 92004) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and an antibody against calcitonin gene-related peptide (CGRP; polyclonal rabbit anti-rat, 1:5000; #C8198, AB_259091; Sigma-Aldrich, St. Louis, MO, USA) or growth associated protein-43 (GAP43 ...
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bioRxiv - Cell Biology 2023Quote: ... Beads were washed two times with 1 mL Pierce Lysis Buffer using a magnetic rack and eluted in 100 μl Pierce lysis buffer containing 3X FLAG® Peptide (Sigma, Cat. # F4799) at 20 μM ...
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bioRxiv - Cell Biology 2023Quote: ... the Anti-Flag M2 Affinity Gel was incubated with 2x vol of 100 µg/ml Flag-Peptide (in TBS, Sigma-Aldrich, cat.no. F3290) at 4 °C and 350 rpm agitation for 30 min ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were isolated from the spleen and lymph nodes of OT-II Cas9 double-transgenic mice or Cas9 transgenic mice and activated with splenocytes irradiated at 30Gy and OVA peptide (10mg/mL) (Sigma Aldrich O1641-5mg) for OT-II lung inflammation model ...
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bioRxiv - Neuroscience 2024Quote: ... QuickCIP was heat inactivated at 80 °C for 2 min and the peptide solutions were desalted using C18 StageTips (Empore C18 SPE Disks, Sigma Aldrich, Darmstadt, Germany). Briefly ...
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bioRxiv - Biophysics 2021Quote: NmeCas9 was expressed in Rosetta 2(DE3) cells (Millipore Sigma, 71400-3) and grown in LB supplemented with 100 µg/mL Carbenicillin and 34µg/mL Chloramphenicol ...
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bioRxiv - Cell Biology 2020Quote: ... a thymidine block was performed by adding 3 mM thymidine (Sigma-Aldrich) for 16 h ...
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bioRxiv - Systems Biology 2020Quote: ... and 0.25 U/µl benzonase (Millipore Benzonase Nuclease HC, Ref#71206-3). Concentrated stocks of the lysis buffer were always freshly prepared and added volumes were adjusted to the remaining PBS supernatant in the cell pellets ...
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bioRxiv - Cell Biology 2019Quote: ... and 500 μM auxin (3-indole-acetic acid, IAA, Sigma, I-5148) for optimal depletion of SAF-A-AID-mCherry.
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bioRxiv - Immunology 2021Quote: ... it was added the substrate 3-Amino-9-ethylcarbazole (AEC) (Sigma-Aldrich), for 20 minutes ...
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PD-1 checkpoint blockade disrupts CD4 T cell regulated adaptive B cell tolerance to foreign antigensbioRxiv - Immunology 2021Quote: ... and developed with chromogen substrate: 0.03% 3-amino-9-ethylcarbazole (Sigma-Aldrich) and 0.015% H2O2 in 1M sodium acetate ...
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bioRxiv - Cell Biology 2019Quote: ... rerio embryos with or without chorion were mounted in 3 % methylcellulose (Sigma) in a small petri dish ...