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Citations for Millipore Sigma :
4951 - 5000 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... Linoleic Acid (EIC) (Sigma-Aldrich, L1376, CAS number: 60-33-3) were prepared immediately prior to use from DMSO stocks (100 mM) ...
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bioRxiv - Immunology 2024Quote: ... Sodium Citrate (CAS--6132-04-3) were obtained from Sigma-Aldrich. SR 2211 ...
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bioRxiv - Cell Biology 2024Quote: ... -Trp (-HLT) agar plates containing 3-Amino-1,2,4-triazole (Sigma, A8056) at the indicated dilutions ...
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bioRxiv - Cell Biology 2024Quote: ... rabbit polyclonal anti-integrin beta 3 (1:500, Millipore, Cat# AB2984), rabbit polyclonal anti-integrin beta 4 (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were fixed with a PBS buffer containing 3% paraformaldehyde (Sigma) and 2% sucrose for 10 mins ...
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bioRxiv - Immunology 2024Quote: ... centrifuged for 5 min at 500 g (Centrifuge 3-18KS, Sigma), then resuspended in full media to a concentration of 1 × 106 cells/mL and distributed in 96-well plates (100 μL/well) ...
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bioRxiv - Microbiology 2024Quote: ... 8% polyacrylamide gel containing 0.5% 3- (Acrylamido) phenylboronic acid (Sigma-Aldrich) after resuspending in a 2X RNA Loading Dye (NEB) ...
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bioRxiv - Developmental Biology 2024Quote: ... the sections were blocked with 3% bovine serum albumin (Sigma-Aldrich), 10% goat serum ...
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bioRxiv - Neuroscience 2024Quote: ... were embedded in 3% (w/v) aqueous agarose (Sigma-Aldrich A4018). Agarose-stabilized samples were cut into 0.5-1 mm3 cubes and prepared for electron microscopy.
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bioRxiv - Bioengineering 2024Quote: ... washed twice with 3 % bovine serum albumin (BSA, Sigma-Aldrich, #A7906) in PBS for 5 min ...
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bioRxiv - Bioengineering 2024Quote: ... cells were blocked with 3% normal donkey serum (Sigma, S30-M). Primary antibodies were against PAX6 ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by inactivation of endogenous peroxidases using 3% hydrogen peroxide (Sigma) for 10 min ...
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bioRxiv - Bioengineering 2024Quote: A 3 mM solution of Fak Inhibitor 14 (Millipore Sigma SML0837) was made using PBS ...
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bioRxiv - Neuroscience 2024Quote: ... and 2–3 µg/kg/day diphtheria toxin (D0564, Sigma-Aldrich) were intraperitoneally injected into PdgfraCreER/+;Rosa26DTR/+(Depl ...
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bioRxiv - Neuroscience 2024Quote: ... the BMP inhibitor Dorsomorphin (DM, 3 µM, cat #P5499, Sigma Aldrich), and the WNT pathway inhibitor IWR-1 (3 µM ...
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bioRxiv - Genomics 2024Quote: ... 3) the Slim Initiative in Genomic Medicine for the Americas (SIGMA), and 4 ...
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bioRxiv - Microbiology 2024Quote: ... Standard solutions were prepared using poly (3-hydroxybutyric acid) (SIGMA Aldrich) and mcl-PHAs from Pseudomonas putida KT2440 ...
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bioRxiv - Immunology 2024Quote: ... cells were treated with 3 mL RBC lysis buffer (Sigma-Aldrich) for 4 min to remove red blood cells ...
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bioRxiv - Cancer Biology 2024Quote: ... and solvent control [1% DMSO + 3% Kolliphor EL (Sigma-Aldrich, #C5135) + 96% PBS] was administered by intraperitoneal injection at 10 mg/kg/day for 4 weeks starting on the day of tumor initiation.
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 3% polyvinylpyrrolidone (PVP, Sigma-Aldrich-Aldrich, 9003-39-8) in the presence of 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... cells were fixed for 3 hours with formalin 10% (Sigma Aldrich), and permeabilized for 20 min with 0.1% Triton X-100 (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 U glutathione reductase (G3664, Sigma; 10 U/3 mL), followed by the addition of 60 µL NADPH (N7505 ...
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bioRxiv - Immunology 2024Quote: ... Erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA) (#E114; Sigma-Aldrich) was used to inhibit ADA1 activity ...
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bioRxiv - Microbiology 2024Quote: ... We used 3-amino-9-ethylcarbazole (AEC) (Sigma-Aldrich, Steinheim, Germany) to visualize protein binding ...
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bioRxiv - Synthetic Biology 2024Quote: ... 100 μL 3% hydrogen peroxide solution (Sigma, catalog #88597-100ML-F) was added to the solution followed by thorough mixing ...
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bioRxiv - Molecular Biology 2020Quote: ... The following antibodies used for immunocytochemistry as described above are as follows: mouse monoclonal antibody to FLAG peptide (Sigma #F1804, 1:500), sheep polyclonal to TGN46 (Biorad #AHP500G ...
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bioRxiv - Molecular Biology 2020Quote: The following antibodies used for immunoblot as described above are as follows: mouse monoclonal antibody to FLAG peptide (Sigma #F1804, 1:5000), Rabbit monoclonal to c-Myc (abcam #ab32072 ...
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bioRxiv - Bioengineering 2019Quote: ... Hela cells from the same batch of 2D culture were seeded into the peptide hydrogel with density ~ 8 × 104 which was measured by an automated cell counter (Millipore Corporation, PHCC3000). The 3D culture was maintained using a 24-well cell culture plate with ~ 390 μL cell suspension ...
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bioRxiv - Cancer Biology 2021Quote: ... and calmodulin-binding peptide (CBP) was affinity-purified by using streptavidin-conjugated agarose beads (EZview™ Red Streptavidin Affinity Gel, Sigma-Aldrich). The initial protein peaks containing EP400NL were collected and pooled together after the size exclusion chromatography and incubated with streptavidin affinity beads at 4 °C overnight with constant rotation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Plasma insulin and C-peptide were measured with mouse/rat ELISAs (EZRMI-13K and EZRMCP2-21K, respectively, EMD Millipore, Billerica, MA, USA). Plasma non-esterified fatty acids (NEFA ...
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bioRxiv - Biophysics 2022Quote: ... about 100 μL supernatant was left and the bound protein was eluted by incubation with 10 μL FLAG peptide (Sigma-Aldrich F3290) at 4°C for 3 hours ...
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bioRxiv - Biochemistry 2021Quote: ... 12 μg peptides were adjusted to pH 7-8 by adding one volume of 50 mM ammonium bicarbonate (ABC, Sigma-Aldrich, A6141). Offline fractionation by high-pH reversed-phase chromatography was carried out on an Ultimate 3000 HPLC (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The source voltage and focusing voltages were tuned for the transmission of MRFA peptide (m/z 524) (Sigma-Aldrich, St. Louis, MO). Data-dependent analysis was carried out in orbitrap-IT parallel mode using CID fragmentation on the five most abundant ions in each cycle ...
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bioRxiv - Biophysics 2020Quote: ... Approximately 10 pmol of peptides were loaded onto the Ascentis Peptides ES-C18 column (2.1 mm x 100 mm, 2.7 μm particle size; Sigma-Aldrich, St. Louis, MO) at 0.400 mL/min flow rate and were eluted with the following gradient ...
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bioRxiv - Genetics 2020Quote: ... The Rad21l1 peptide was concentrated to a final concentration of 1 mg/ml in PBS using a 10 kDa centrifugal filter (Sigma, Catalog # UFC901008). The Rad21l1-derived peptide was injected into three guinea pigs by Pocono Rabbit Farm and Laboratory following the 91-day polyclonal antibody production protocol.
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bioRxiv - Biochemistry 2020Quote: ... and proteins were eluted with 4 mL of the same buffer but containing 100 μL (5 mg mL−1) Flag peptide (Sigma-Aldrich F4799). The eluate was spin concentrated (Sigma-Aldrich CLS431485-251A ...
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bioRxiv - Neuroscience 2021Quote: ... This antiserum detects human α-CGRP and β-CGRP but does not cross-react with any other peptide (data supplied by Sigma). We used a rabbit polyclonal antibody (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... As a positive control we used a bona fide Src substrate peptide corresponding to amino acids 6-20 of p34cdc2 (Millipore-Sigma, 12-140). The reactions were carried out at room temperature in a total volume of 25 μL for 40 min in white 96-well ...
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bioRxiv - Microbiology 2022Quote: ... Samples were washed 3 times with 500 µL of lysis buffer and bound protein was eluted with 50 µl of elution buffer (100 µg/mL FLAG peptide (Sigma-Aldrich #F3290), 50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Immunology 2023Quote: ... the complex was collected in buffer containing 0.2 mg/ml FLAG peptide and concentrated to 400 μL in an Amicon® Ultra-15 Centrifugal Filter Unit (Millipore, Burlington, MA). Complexes were loaded onto a Superose 6 Increase 10/300GL column (GE Healthcare ...
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bioRxiv - Biophysics 2024Quote: ... Peptide samples were loaded onto a Sigma-Aldrich Ascentis Peptides ES-C18 column (2.1 mm x 100 mm, 2.7 µm particle size; Sigma-Aldrich, St. Louis, MO) via an Infinity Autosampler (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 individual peptides (2 μg/ml) or blank control (complete medium with DMSO) onto sterile nitrocellulose MSIP 96-well plates (Millipore, Bedford, MA) coated with capture antibodies against mouse IFN-γ ...
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bioRxiv - Cancer Biology 2023Quote: ... and seeded at 1 x 105 in a 24 well plate and then pulsed with 1ng/mL OVA peptide (Sigma, #S7951-01mg) for 3 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... beads washed five times with pre-chilled NET resuspension buffer and eluted under native conditions by competition using 3x FLAG peptide (Millipore Sigma, F4799). Samples were boiled in the Laemmli SDS sample buffer (Thermofisher ...
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bioRxiv - Immunology 2023Quote: ... were cocultured for 6 hours with autologous herpesvirus papio-immortalized B lymphoblastoid cell lines (B-LCLs) pulsed with Nef peptides (Sigma-Aldrich Japan) at 1 μM concentration or as indicated otherwise under GolgiStop (monensin ...
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bioRxiv - Cell Biology 2024Quote: ... FLAG-tagged proteins were eluted by addition of 40 µL IP buffer supplemented with 500 ng/mL 3xFLAG peptide (Sigma, Cat. # F4799) and incubated for 1 h at room temperature ...
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bioRxiv - Immunology 2023Quote: ... 2.5 x 105 splenocytes from OT-1 mice were seeded in one ml of complete media in 24 well plates and 0.5µg/ml of peptide (Sigma-Aldrich, cat. number S7951). After 48 hours blasts were expanded with 100 U/ml of rmIL-2 in fresh media and harvested on day four or six after initial stimulation.
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bioRxiv - Immunology 2023Quote: ... Cells were incubated in the presence of either 1 μg/mL OVA peptide (323-339; pOVA) (Sigma-Alrdrich; St Louis, MO, USA), 2.5 μg/mL concanavalin A (ConA ...
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bioRxiv - Biochemistry 2024Quote: ... the gel was washed with 10 column volumes of lysis buffer and subsequently eluted with 100 µg/mL free Flag peptide (Sigma Aldrich, F3290) in lysis buffer ...
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bioRxiv - Plant Biology 2021Quote: ... blocked with 3% milk for 1 hour at room temperature and probed with anti-FLAG M2 monoclonal antibody (mouse, Sigma F1804, 1:2000 in 3% milk/PBST), or blocked with 5% BSA for 1 hour at room temperature and probed with anti-HA antibody (rabbit ...