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Citations for Millipore Sigma :
5001 - 5050 of 7743 citations for Recombinant Salmon il21 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and bands were normalized to total protein and the housekeeping gene Gapdh (1:10,000, Millipore, NP_002037).).
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bioRxiv - Bioengineering 2023Quote: ... fibrinogen stock (4 mg of clottable protein/mL of BMEM, bovine plasma, Type I-S, Sigma) was filter sterilized and mixed with 0.5 U/mL thrombin ...
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bioRxiv - Neuroscience 2023Quote: ... DNA was then added to MagnaChip magnetic protein A/G beads (16-663, Millipore, Burlington, USA) overnight that were incubated with either 5-hmC ...
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bioRxiv - Pathology 2023Quote: ... Proteins were detected with the following primary antibodies: mouse monoclonal anti-CnB (Sigma, 0581, 1:1000), rabbit polyclonal anti-CnA (Merck Millipore ...
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bioRxiv - Developmental Biology 2023Quote: ... detection kit and LI-COR Odyssey platform for western blot analyses of phosphorylated proteins (Millipore Sigma). Fg-Stk36WT and Fg-Stk36AA were immunopurified from HEK293 cells transfected with the Fg-Stk36WT or Fg-Stk36AA construct.
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bioRxiv - Plant Biology 2024Quote: ... Total protein was co-immunoprecipitated using the anti-GFP M2 resin (Sigma-Aldrich, St. Louis, MO) and washed 3 times using immunoprecipitation buffer before analyzing on an SDS-PAGE gel ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal protein amounts were separated on SDS-PAGE and blotted on PVDF membranes (Millipore, Darmstadt, Germany). The membranes were blocked with 10% non-fat dry milk in TBST buffer (10 mM Tris-HCl pH=8.0 ...
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bioRxiv - Microbiology 2023Quote: ... the pellets were washed first with 1× BugBuster® (10× Protein Extraction Reagent Novagen® Merck), then twice with water and once with 50 mM NaH2PO4 (pH 8) ...
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bioRxiv - Immunology 2023Quote: ... The protein antibody used for western blotting was mouse or rabbit anti-6xHis antibodies (Millipore-Sigma). Secondary antibodies were IRDye 680RD goat anti-mouse and/or IRDye 800CW goat anti-rabbit (Li-Cor) ...
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bioRxiv - Immunology 2023Quote: ... The protein antibody used for western blotting was mouse or rabbit anti-6xHis antibodies (Millipore-Sigma). Secondary antibodies were IRDye 680RD goat anti-mouse and/or IRDye 800CW goat anti-rabbit (Li-Cor) ...
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bioRxiv - Immunology 2023Quote: ... Protein bands were visualized by treating the membrane with Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore, Darmstadt ...
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bioRxiv - Molecular Biology 2023Quote: ... Ifi205 proteins corresponding fractions were combined and purified by ANTI-FLAG M2 Affinity Gel (Sigma, A2220). A small portion of the final proteins were run through the Superdex 200 columns (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... The purification of monoclonal antibodies was performed by affinity chromatography using Hitrap Protein G Column (Sigma) starting from 150 ml of hybridoma cell supernatant ...
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bioRxiv - Biophysics 2023Quote: ... Protein in the supernatant was quantified on a plate reader using the Bradford assay (Sigma Aldrich). All samples were prepared in loading dye (NU-PAGE dye + 5mM BME ...
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bioRxiv - Biophysics 2023Quote: ... The eluted proteins were concentrated to 1 ml using Amicon Ultra-15 10k-cutoff filter (Millipore) and loaded onto a HiLoad Superdex 200 prep grade column (GE Healthcare ...
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bioRxiv - Biophysics 2023Quote: ... The eluted proteins were concentrated to 1 ml using Amicon Ultra-15 3k-cutoff filter (Millipore) and loaded onto a HiLoad Superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... followed by the addition of 100 ng of active SLO protein reduced by TCEP (Sigma Aldrich) and 5 ug of corresponding IgG and/or IgG fragments ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were centrifuged as described above and protein concentrations were quantified via Bradford assay (Sigma-Aldrich). Approximately 20-30 μg of protein was resolved on 8% polyacrylamide gels and transferred onto nitrocellulose membranes using semi-dry Trans-Blot TurboTM (Bio-Rad) ...
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bioRxiv - Bioengineering 2023Quote: ... was prepared at a concentration of 5 mg/mL by dissolving fibrinogen protein (crystals, Sigma-Aldrich) in sterile phosphate-buffered saline (PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Flag-tagged proteins were eluted from the beads using 150 ng/ml of 3xFLAG peptide (Sigma) in 50–100 ul of elution buffer (20 mM HEPES-KOH (pH 7.5) ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein sample was then reduced with 100 µl of 10 mM dithiothreitol (Sigma Aldrich, UK) in denaturation buffer for 30 min at ambient temperature ...
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bioRxiv - Microbiology 2023Quote: ... incubated with mouse anti-HA mAb clone 12CA5 conjugated with protein G agarose beads (Sigma-Aldrich) for 16 h at 4°C ...
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bioRxiv - Immunology 2023Quote: ... anti-Myc agarose affinity beads (A7470) and protein A/G agarose affinity beads (P6486/E3403) (Sigma); mouse recombinant M-CSF (315–02 ...
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bioRxiv - Biophysics 2023Quote: ... The proteins were then labeled with 25-fold molar excess monoreactive maleimide-Dibenzocyclooctyne (DBCO) (Sigma Aldrich) in Phosphate Buffered Saline (PBS ...
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bioRxiv - Biochemistry 2024Quote: ... Soluble protein fractions were mixed with FLAG-M2-agarose beads (A2220, Sigma-Aldrich, St. Louis, MI) pre-equilibrated with buffer A ...
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bioRxiv - Biochemistry 2024Quote: ... The resin-bound His-tagged proteins were washed with wash buffer containing 20mM imidazole (Sigma #12399) to eliminate non- specific binding and eluted 5 times subsequently with elution buffer (20mM Tris HCl pH7.5 ...
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bioRxiv - Genomics 2023Quote: ... total protein was concentrated and size-selected by passing through an Amicon Ultra 10K column (Millipore), retaining the high molecular weight fraction ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein was separated using 10%/12% SDS-PAGE and transferred to polyvinylidenedifluoride membranes (Immobilon FL, Millipore). After blocking ...
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bioRxiv - Developmental Biology 2023Quote: Proteins were separated on 5% SDS-PAGE gels and transferred to PVDF membranes (Millipore, Bedford, MA). After blocking with 5% fat-free milk + TBS ...
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bioRxiv - Plant Biology 2024Quote: ... the total proteins were drawn by extraction buffer with addition 2% protease inhibitors cocktail (Sigma, P9599) and 2% phosphatase inhibitors cocktail (Yeasen,20109-A) ...
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bioRxiv - Physiology 2024Quote: ... The protein samples were denatured using 1x Laemmli sample buffer with 2.5% 2-mercaptoethanol (M3148, Sigma) for each sample at 95 °C for 5 min (STIM1 ...
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bioRxiv - Physiology 2024Quote: ... Proteins (15µg) were separated in SDS-PAGE gel by electrophoresis and transferred onto PVDF membrane (Millipore). Membranes were blocked with 5% nonfat dry milk (Cell signaling ...
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bioRxiv - Neuroscience 2023Quote: ... The seven target proteins and antibodies for the sFANSeq panel included Neuronal Nuclei Antigen (NeuN, Millipore Cat# ABN91 ...
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bioRxiv - Plant Biology 2024Quote: ... SnRK2s-GFP as well as phosphorylated HUB2 proteins by using anti-PAP (1:1000, SIGMA, P1291), anti-GFP (1:2000 ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were lysed in RIPA buffer supplemented with protein inhibitor cocktail (Sigma-Aldrich, St. Louis, MO). Total protein concentration was quantified by the Bradford method ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 1% FBS (v/v) and protein expression enhancing sodium butyrate (66) solution (SIGMA-ALDRICH) to a final concentration of 2 nM were added to the cells to make up to the final volume of transfection ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: We extracted total protein from cells using RIPA lysis and extraction buffer (Millipore Sigma. Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... The final protein was then concentrated by using Amicon Ultra 30 kDa MWCO filters (Millipore Sigma).
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bioRxiv - Cancer Biology 2024Quote: ... The separated proteins were then electro-transferred on 0.45-m polyvinylidene difluoride membranes (Millipore, Billerica, USA). The membranes were subsequently blocked using 5% skim milk for two hours at room temperature after being washed three times with TBST ...
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bioRxiv - Biophysics 2024Quote: ... The eluted protein was concentrated with a 15 ml 10000 MWCO spin filter (Merck Millipore Ltd.). The sample was centrifuged at 20.000 g for 15 min to remove precipitated protein prior to being loaded onto a HiLoad 16/600 Superdex 200 size exclusion chromatography (SEC ...
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bioRxiv - Biophysics 2024Quote: Three different buffers were used in DHFR protein purification: Lysis buffer [100 mM bicine (Sigma-Aldrich), 5 mM imidazole (Millipore-Sigma OmniPur®) ...
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bioRxiv - Biophysics 2024Quote: The purified protein was concentrated by using an Amicon Ultra 0.5 mL 50 kDa filter (Millipore) at 12,000 ×g for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were then transferred on a PolyVinyliDene Fluoride (PVDF) membrane (Immobilon-P, Merck Millipore, Burlington, USA) using liquid transfer with 20 % methanol at 100V ...
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bioRxiv - Cancer Biology 2024Quote: ... Antibodies to the following proteins were used in the immunoblots: puromycin (Millipore, #AF488, 1:2000; RRID:AB_2737590), 4E-BP1 (Cell Signaling ...
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bioRxiv - Biophysics 2024Quote: ... The denatured protein was then reduced with 5 μL of 500 mM dithiothreitol (DTT; Sigma-Aldrich) at room temperature for 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... Staining was performed against glial fibrillary acidic protein (mouse anti GFAP−Cy3™, Sigma-Aldrich, C9205), Secondary antibody was anti-mouse Alexa Fluor 488 (Thermo-Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... and equilibrated before FLAG tagged proteins were immunoprecipitated using anti-Flag M2 agarose beads (Sigma #A2220).
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bioRxiv - Cell Biology 2024Quote: ... and then the proteins in the gel were transferred onto Immobilon-FL polyvinylidene difluoride membranes (Millipore). The membranes were blocked with BlockPRO Protein-free Blocking Buffer (Visual Protein ...
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bioRxiv - Molecular Biology 2024Quote: ... the instrument was calibrated using a protein standard containing 10 nM β-amylase (Sigma Aldrich A8781) and 3 nM Thyroglobulin (Sigma-Aldrich 609310) ...
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bioRxiv - Immunology 2024Quote: ... HLA-G2 proteins in PBS were concentrated by Amicon Ultra-15 (NMWL 10,000 Da) (Merck Millipore), and the flowthrough solution from protein concentration was used as a negative control (PBS ...