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Citations for Millipore Sigma :
4951 - 5000 of 7743 citations for Recombinant Salmon il21 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: Proteins were separated on 5% SDS-PAGE gels and transferred to PVDF membranes (Millipore, Bedford, MA). After blocking with 5% fat-free milk + TBS ...
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bioRxiv - Biochemistry 2024Quote: ... Soluble protein fractions were mixed with FLAG-M2-agarose beads (A2220, Sigma-Aldrich, St. Louis, MI) pre-equilibrated with buffer A ...
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bioRxiv - Biochemistry 2024Quote: ... The resin-bound His-tagged proteins were washed with wash buffer containing 20mM imidazole (Sigma #12399) to eliminate non- specific binding and eluted 5 times subsequently with elution buffer (20mM Tris HCl pH7.5 ...
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bioRxiv - Microbiology 2023Quote: ... Bound protein was eluted in 30 μl of elution buffer (0.15 mg/ml 3xFLAG peptide [Sigma, St Louis ...
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bioRxiv - Immunology 2023Quote: ... Eluted proteins were then dialyzed and concentrated using an Amicon Ultra-15 10 KDa cutoff (Millipore).
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bioRxiv - Neuroscience 2023Quote: ... Protein samples were separated by SDS-PAGE (15%) and transferred to PVDF membranes (Immobilon-FL, Millipore). Membranes were incubated with Intercept Blocking Buffer (Li-COR) ...
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bioRxiv - Pathology 2023Quote: ... Proteins were separated using 7.5% SDS-PAGE gels and blotted onto a PVDF membrane (Millipore, 0.45μm) and blocked followed by incubation with anti-FAK (CST) ...
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bioRxiv - Biochemistry 2023Quote: ... Purified protein was concentrated using an Amicon Ultra-15 centrifugal filter unit (Millipore, 50 kDa MWCO) and flash frozen in liquid nitrogen.
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bioRxiv - Microbiology 2023Quote: ... and the amount of total protein in the samples was quantified using the Bradford assay (Sigma). Subsequently ...
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bioRxiv - Cell Biology 2023Quote: ... FLAG-tagged proteins were eluted from the beads with 200 µg/ml FLAG peptide (Sigma-Aldrich) in 0.5 ml of lysis buffer B ...
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bioRxiv - Microbiology 2023Quote: ... The flow-through was then applied to a Protein G Sepharose (Millipore Sigma, #GE17-0618-01) containing column (5ml polypropylene columns ...
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bioRxiv - Immunology 2023Quote: ... The refolded protein product was concentrated by centrifugation in 3K MWCO Amicon tubes (EMD Millipore, #UFC900324) until a final concentration of at least 4 mg/mL was reached ...
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bioRxiv - Molecular Biology 2023Quote: ... Cleared lysates with equal amounts of proteins were transferred to anti-FlagM2 affinity gel (Sigma-Aldrich) and incubated for 1.5 hours at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted proteins were separated from beads with Corning Costar Spin-X filter units (#CLS8162, Sigma-Aldrich) and stored at -20°C until further processing.
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bioRxiv - Cell Biology 2022Quote: ... and immunoprecipitated proteins were eluted from Dynabeads by incubating with 200 μM FLAG peptides (Sigma-Aldrich) for 90 min ...
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bioRxiv - Molecular Biology 2023Quote: Proteins were resolved on SDS-polyacrylamide gels and transferred onto PVDF Immobilon-FL membranes (Millipore, IPFL00010). Membranes were blocked with Intercept blocking buffer in PBS (LI-COR ...
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bioRxiv - Molecular Biology 2023Quote: ... 60ul of protein G Sepharose beads conjugated to M2 Flag antibody (Millipore Sigma Cat. M8823-5ML) was added to each sample ...
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bioRxiv - Cell Biology 2023Quote: ... Immunoprecipitated protein-DNA complexes were prepared using the Magna ChIP A kit (Millipore, Burlington, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the protein was eluted with ten column volumes of elution buffer (LWB including 3mM desthiobiotin (Sigma)) ...
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bioRxiv - Genomics 2023Quote: ... Samples were added to Magna ChIP™ Protein A+G Magnetic Beads (16-663, Sigma-Aldrich) precoated with specific antibody ...
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bioRxiv - Cancer Biology 2023Quote: Protein samples (50 μg) were diluted in 50 mM ammonium bicarbonate buffer (AmBic, NH4HCO3; Sigma-Aldrich), 0.2% (m/v ...
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bioRxiv - Bioengineering 2023Quote: ... with the protein aqueous solution (1 mg/ml of PLL, Sigma-Aldrich, in PBS pH 10.4), at a 1:2 ratio ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were quantified and separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore) according to standard protocols ...
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bioRxiv - Plant Biology 2023Quote: ... and proteins were blotted onto a 0.45 µm PDVF Immobilon-P membrane (Merck Millipore, ref. IPVH00010) after electrophoresis ...
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bioRxiv - Plant Biology 2023Quote: ... Protein expression was induced at 20 hours post-infiltration by spraying using indicated estradiol (Sigma-Aldrich) concentration and 0.001% (v/v ...
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bioRxiv - Cell Biology 2023Quote: HA-METTL3-Flag protein was purified from 293T by using anti-HA magnetic beads (Sigma Aldrich) as described in this method ...
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bioRxiv - Molecular Biology 2023Quote: ... the lysate in incubated with 50uL of Protein A agarose beads (Millipore Sigma-Aldrich 16-157) for 1 hour at 4°C with rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins extracted from cells were incubated overnight at 4°C with FLAG-M2 beads (Sigma, A2220) or with antibody of interest ...
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bioRxiv - Neuroscience 2023Quote: ... γ8 proteins were further concentrated using Amicon Ultra centrifugal filter with MWCO 3K (Merck Millipore UFC500324).
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bioRxiv - Immunology 2023Quote: ... Expression of the proteins of interest was induced by 3 μg/ml doxycycline (dox, Sigma-Aldrich) added to the culture medium ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were detected using chemiluminescence from horseradish peroxidase-coupled secondary antibodies Anti-rabbit HRP (Sigma; A0545), 1:15,000.
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bioRxiv - Cell Biology 2023Quote: ... The proteins were transferred overnight onto methanol activated polyvinylidene difluoride membrane (PVDF, Sigma-Aldrich, MO, USA) in Transfer Buffer (25 mM Tris ...
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bioRxiv - Bioengineering 2022Quote: ... Protein bound to the wells were detected with a peroxidase conjugated anti-Flag antibody (Sigma A8592) and quantified with QuantaBlu™ Fluorogenic Peroxidase Substrate Kit (Thermo Scientific™ 15169) ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were eluted in the same buffer conaining additionally 20 mM glutathione (pH 7.5) (Sigma-Aldrich). GST was removed by the addition of Presission protease in a ratio of 1:100 ...
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bioRxiv - Cancer Biology 2022Quote: ... All proteins were separately concentrated using 10 kD cut-off Amicon Ultra-15 Centrifugal filters (Millipore) and applied to a final gelfiltration run on a Superdex 75 column (Cytivia ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were separated on SDS-PAGE (8%) transferred to PVDF membranes (Immobilon-P, 0.45 μm, Millipore) using wet transfer in transfer buffer (48 mM Tris base ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal protein concentrations were run on an SDS-PAGE gel and transferred to PVDF (Sigma IPFL00010). PVDF membranes were blocked in 5% milk-PBST and then probed with NRAS (1:250 ...
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bioRxiv - Cell Biology 2023Quote: ... TEV-eluted supernatant was added to 100 µL of S-protein agarose (69704-3, EMD Millipore) to capture S-tagged protein ...
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bioRxiv - Cell Biology 2023Quote: ... The ANKRD26 fragments were expressed as a GST fusion protein in Rosetta2 competent cells (#71402, Millipore) and purified using Glutathione Sepharose™ 4B Media (17075605 ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 uL of pre-washed magnetic beads (Magna ChIP Protein A+G Magnetic Beads, Millipore Sigma) were added to each sample for 2 hours at 4º C ...
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bioRxiv - Cancer Biology 2023Quote: ... For mRNA or protein decay analyses cells were treated with actinomycin D (5 µM, Sigma Aldrich) or emetin (100 µM ...
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bioRxiv - Cancer Biology 2023Quote: ... Denatured proteins were separated by SDS-PAGE and transferred onto a PVDF membrane (Millipore, Massachusetts, USA). After blocking ...
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bioRxiv - Cancer Biology 2022Quote: ... 50µL of protein G beads were coated 2ug of anti-AGO2 antibody (Sigma SAB4200085 Lot# 0000089486) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 30 μg of total protein sample was mixed with Laemmli buffer (180 mM DTT (Sigma #D9779), 4% SDS (VWR #442444H) ...
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bioRxiv - Genetics 2023Quote: ... Supernatant was collected and protein concentrations were determined using Bradford Reagent (Sigma-Aldrich, cat no. B6916). Protein samples were boiled with 4x Laemmli sample buffer (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... Metabolites were separated from proteins and exosomes using a 3 kDa MWCO centrifugation unit (UFC910024, Millipore) and centrifuged at 4000 × g for 40 min at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... The HAHis-tagged SbtB proteins were detected with monoclonal antibodies against the HA-epitope (Sigma, USA). Secondary ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 µg of proteins were incubated with 30 µl of Streptavidin-Agarose beads (Sigma-Aldrich, CS1638) on a rotating wheel for 3 hrs at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... The protein fractions were pooled and concentrated in a 10 kDa cut-off Amicon filter (Millipore). Further protein purification was performed by size-exclusion chromatography using SuperdexTm 200 increase 10/300 column (Cytiva) ...
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bioRxiv - Biochemistry 2023Quote: ... the surviving colonies were analysed for target protein expression using tetracycline hydrochloride (Cat#T3383; Sigma-Aldrich). Stable cells expressing full length and truncated versions of GFP-SCAF1 were generated in U-2 OS Flp-In T-Rex cells stably expressing mCherry-XRCC1 ...