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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for Recombinant Mouse Melanoma Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... were cultured in the presence of recombinant mouse IL-4 (25 ng/mL; #404-ML, R&D) and LPS (20 μg/mL; #L2880, Sigma) over night to induce B cell activation and terminal differentiation ...
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bioRxiv - Microbiology 2023Quote: ... 21 and 42 with 50 μg of recombinant LMW630 mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, MO, USA). Enzyme-linked immunosorbent assay was performed to measure serum responses to antigen (see methods below ...
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bioRxiv - Molecular Biology 2024Quote: ... Further confirmation of purification of the recombinant protein was obtained by immunoblots probed with mouse anti-polyhistidine IgG (Sigma-Aldrich) and goat anti-mouse alkaline phosphatase-conjugated antibodies (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were then washed with buffer and recombinant Tau protein (Sigma recombinant Tau protein #T0576) and ATP (Sigma #A1852 ...
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bioRxiv - Cell Biology 2021Quote: ... peroxidase-anti-peroxidase for detecting TAP-tagged proteins (Sigma); anti-FLAG mouse monoclonal antibody (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... and dextran tagged with fluorescein (40,000 MW, Sigma Aldrich) were resuspended in 100 μl HBSS (4 mg/ml ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-tagged protein was immunoprecipitated using FLAG antibody (Sigma) and Protein G magnetic beads (Invitrogen) ...
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bioRxiv - Genetics 2019Quote: ... FLAG-tagged protein was immunoprecipitated using FLAG antibody (Sigma) and Protein G magnetic beads (Invitrogen) ...
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bioRxiv - Developmental Biology 2019Quote: Biotin-tagged OSTN was labeled by Cy3-streptavidin (Sigma). Equal volume of 25 μM biotin-tagged OSTN and 0.1 mg/ml Cy3-streptavidin were incubated with on ice for 15 minutes at 4 C ...
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bioRxiv - Molecular Biology 2023Quote: ... HTP-tagged proteins were detected using rabbit PAP (Sigma) diluted 10,000 fold ...
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bioRxiv - Cancer Biology 2021Quote: ... pTB146 His-SUMO-mSTING (residues 139-378) was expressed in Rosetta (DE3) pLysS competent cells (Sigma-Aldrich). Cells were grown in 2×YT medium with 100 μg/mL ampicillin until they reached an OD600 of 1 ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... plasmid coding for 6x-His-Cas9 were transformed in Rosetta DE3 Novagen competent cells (Merck Millipore, USA). Protein production was induced using autoinduction medium (Formedium ...
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bioRxiv - Cancer Biology 2023Quote: ... pTB146 His-SUMO-mSTING (residues 139-378) was expressed in Rosetta (DE3) pLysS competent cells (Sigma-Aldrich). Cells were grown in 2xYT medium with 100 μg/mL ampicillin until they reached an OD600 of 1 ...
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bioRxiv - Molecular Biology 2022Quote: ... and expression levels of AGAL-FLAG tagged proteins were assessed by Western blot with mouse monoclonal anti-FLAG antibody (F1804, SIGMA-Aldrich, Prague, Czech Republic). Clones demonstrating similar GLA_FLAG transcript and AGAL-FLAG protein amounts were selected for further analyses.
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bioRxiv - Molecular Biology 2023Quote: ... Histidine tagged PDE4 and GST tagged CP proteins were detected chromogenically using anti-mouse secondary antibodies labelled with alkaline phosphatase (1:30000) (cat#A3562, Millipore Sigma, Burlington, MA, USA) and western blue stabilized substrate for alkaline phosphatase (Promega ...
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bioRxiv - Genetics 2023Quote: ... The presence of the tagged protein was identified by immunoblotting using anti-FLAG antibody (F1804 monoclonal antibody, clone M2 in mouse, SLCK5688, Sigma Aldrich, Supplemental Fig 2F). Rescue was confirmed by measuring cilia length ...
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bioRxiv - Neuroscience 2021Quote: ... cells were stimulated with human TNF recombinant (10 ng/ml) (Sigma-Aldrich Corporation, Missouri, EUA) for 24 hours.
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bioRxiv - Molecular Biology 2023Quote: ... Purified recombinant proteins were analyzed by SDS-PAGE and concentrated using stirred ultrafiltration cells (Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... the following antibiotics or small molecules were used: 50 µg/mL ampicillin (Amp - Sigma-Aldrich), 25 µg/mL kanamycin (Kan - Sigma-Aldrich ...
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Contractile acto-myosin network on nuclear envelope remnants positions human chromosomes for mitosisbioRxiv - Cell Biology 2019Quote: Small molecule inhibitors were used with the following concentrations: 1NM-PP1 1µM (Merck Millipore 529581), RO-3306 10µM (Merck Millipore 217699) ...
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bioRxiv - Biophysics 2021Quote: ... Excess mSA molecules were removed by passing the sample through biotin–conjugated agarose resin (Sigma). Free thiols on GlpG were alkylated by iodoacetamide (10 mM) ...
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bioRxiv - Cancer Biology 2023Quote: ... Apoptosis was detected using FITC labeled VAD-FMK molecule to detect activated caspases (Sigma-Aldrich), or by Annexin V-APC staining (Ebioscience ...
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bioRxiv - Genomics 2024Quote: ... such as a community of DNA molecules from calf thymus (Sigma-Aldrich, St. Louis, MO). For this experiment ...
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bioRxiv - Microbiology 2019Quote: An anti-adhesion coating can be produced in one of two ways: (1) Treatment with hydroxypropylmethylcellulose (HPMC, H8384, Sigma-Aldrich). The leaf imprint was treated with 0.3% w/v HPMC in phosphate buffer saline (PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... 3533-005-02) and overlaid with Organoid Media (Supplementary file 1) in 6-well ultra-low adhesion plates (Sigma, CLS3471). H2B:GFP retrovirus (91 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A first purification step was performed using immobilized metal affinity chromatography with a His-bind resin column (His-Bond kit, Novagen) to recover PGM variants ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was eluted in a gradient of His-AC washing buffer and His-AC elution buffer (both supplemented with 0.1% Tween-20 (Sigma Aldrich), 0.1% NP-40 (Thermo Fischer Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... His–NFR1CD–Myc or His–NFR1CD–K351E–Myc and NopT–FLAG–His were co–expressed by using Duet expressing vectors (Novagen) with 0.5 mM IPTG at 28°C for 18 h ...
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bioRxiv - Synthetic Biology 2019Quote: FLAG-tagged encapsulins were purified from HEK293T cells using the ANTI-FLAG M2 Affinity Gel (SIGMA A2220, Sigma-Aldrich) in a batch format according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2019Quote: FLAG-tagged encapsulins were purified from HEK293T cells using the ANTI-FLAG M2 Affinity Gel (SIGMA A2220, Sigma-Aldrich) in a batch format according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... Protein production was confirmed by SDS-PAGE and subsequent Western blotting analysis of a K30 sample after 24 h using an anti-His antibody from mouse (dilution 1:3000, Sigma-Aldrich, cat.no. H1029) and secondary anti-mouse IgG-HRP antibody produced in goat (dilution 1:20,000 ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was sequentially incubated at room temperature for one hour with anti-His and conjugated anti-mouse antibodies (Sigma-Aldrich, Darmstadt, Germany) (Wu et al. ...
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bioRxiv - Microbiology 2020Quote: ... as a His-tag fusion in pET28b (Novagen). Cells were grown in Terrific-Broth at 37°C to an OD600 = 1.2 and after 15 min at 4°C induced with 1 mM isopropyl β-d-thiogalactoside ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were detected using anti-His-HRP (Sigma) and anti-FLAG-HRP (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... -His with or without 3-AT (Sigma-Aldrich).
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bioRxiv - Pathology 2021Quote: ... cloned into a pET28a His-tag vector (Novagen) and expressed in E ...
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bioRxiv - Biophysics 2020Quote: ... His-purifying resin was from Novagen (Darmstadt, Germany). Bis-(sulfosuccinimidyl ...
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bioRxiv - Cell Biology 2021Quote: ... and Anti-His(1:3000) antibody(Sigma #H1029).HRP conjugated mouse IgG was used at 1:4000 dilution(Sigma #A3673 ...
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bioRxiv - Biochemistry 2021Quote: ... anti-His (Sigma H1029; 1:3000 for IB), anti-Hsp70 (Abcam ab47455 ...
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bioRxiv - Plant Biology 2022Quote: ... followed by blotting using Anti-His (SAB4301134, Sigma) and Anti-GST antibody (ab9085 ...
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bioRxiv - Genomics 2023Quote: ... with 10% heat-inactivated (HI) FBS (Sigma F0926). Vero/hSLAM cells were a kind gift from Dr ...
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bioRxiv - Systems Biology 2023Quote: ... MCF7 in DMEM-Hi Glucose medium (Sigma-Aldrich) HCC1937 and ZR-75-1 in RPMI 1640 media (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 2% GlutaMAX (200 mM ...
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bioRxiv - Biochemistry 2024Quote: ... An anti-His-primary antibody (Sigma, 1:2,000) was incubated with the protein-treated lipid strips to detect Jps1 bound via its C-terminal His-tag ...
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bioRxiv - Cell Biology 2024Quote: ... +10% HI FBS(Sigma Aldrich, cat#F4135-500ML) + penicillin/streptomycin (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Base media for the culture of mESCs were supplemented with 1000 units/mL ESGRO Recombinant mouse LIF protein (EMD Millipore ESG1107), and 2i small molecule inhibitors ...
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bioRxiv - Microbiology 2020Quote: ... mouse polyclonal antibody was produced immunizing a mouse with 50 mg of purified recombinant MtEPSPS containing Freund’s incomplete adjuvant (Sigma-Aldrich, USA) (total volume of 100 µL ...
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bioRxiv - Developmental Biology 2022Quote: ... The dish was then incubated with 40 µg/mL mouse E-cadherin recombinant protein (8875-EC-050, Bio-Techne) or 40 µg/mL fibronectin (F1141, Sigma-Aldrich) at 4 □ overnight ...