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Citations for Millipore Sigma :
701 - 750 of 10000+ citations for Recombinant Mouse Melanoma Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... un-spliced precursor and mutant SufB proteins carrying an N-terminal 6X-His-tag were over-expressed in BL21 (DE3) E.coli cells via IPTG (500µM) (Sigma 367-93-1) induction at 37°C for 4hrs ...
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bioRxiv - Neuroscience 2024Quote: ... The tissues were mashed through a 70-μm cell strainer in PBS / 2% heat-inactivated fetal bovine serum (HI-FBS; Sigma-Aldrich) / 10 mM EDTA-Na (pH 8.0) ...
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bioRxiv - Cell Biology 2020Quote: Mouse tracheal epithelial cells (mouse AECs) were isolated after pronase (Sigma-Aldrich, 9036-06-0) digestion of WT and Atg16L1HM mouse tracheas and grown on supported membrane inserts (Corning #3460 and #3470 ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were induced to express the indicated BioID2-tagged proteins by the addition of 0.2 μg/ml doxycycline (Sigma-Aldrich, St. Louis, MO) for 16 hours ...
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bioRxiv - Plant Biology 2021Quote: The inhibitory effect of recombinant P235 on recombinant glycosyl hydrolase was assayed using the β-Glucosidase Activity Assay Kit (MAK129, Sigma). Enzymatic reactions were carried out in K-Phosphate buffer (100 mM ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GST-mm-FMN2-eFSI (GST-FMN2-eFSI) proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant E1E2 was extracted from cell membranes using 1% NP-9 and purified via sequential Fractogel EMD TMAE (Millipore), Fractogel EMD SO3− (Millipore) ...
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bioRxiv - Biochemistry 2022Quote: Expression of recombinant extracellular proteins was performed by transient transfection in HEK−293F cells using polyethylenimine (PEI, Sigma Aldrich). 300 mL of culture at a density of 1.0 × 106cells/mL was transfected with 300 μg DNA using 450 μg PEI ...
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bioRxiv - Bioengineering 2021Quote: ... we PEGylated our microspheres by functionalizing carboxylic acid groups on the microsphere’s surface with 2,000 Da Poly(ethylene)glycol (PEG) diamine molecules (Sigma, 753084) via EDC chemistry ...
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bioRxiv - Biochemistry 2020Quote: ... The column collection system was evaluated using three representative molecules: methadone (0.001 ng/mL, Sigma-Aldrich, St. Louis, MO), 1,2-Dipalmitoyl-sn-glycero-3-phosphorylcholine (0.1 ng/mL ...
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bioRxiv - Plant Biology 2021Quote: ... for detection of FLS2-BAK1 co-immunoprecipitation or anti-rabbit IgG (whole molecule)–HRP (A0545, Sigma, dilution 1:10000) for all other western blots.
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bioRxiv - Microbiology 2021Quote: The following small molecule inhibitors and antibodies were used for TEER and EGL inhibition assays: ATN-161 (Sigma, SML2079), SB 431542 hydrate (Sigma ...
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bioRxiv - Biochemistry 2019Quote: ... the levels were measured using the Single Molecule Counting (SMC®) Immunoassay Technology using commercially available kits from Millipore / Sigma (Aβ1-40 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... IVM was converted into a fluorescent molecule using N-methylimidazole and trifluoroacetic anhydride (Sigma Chemical, St Louis, MO, USA). An aliquot (100 μl ...
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bioRxiv - Molecular Biology 2020Quote: ... The total RNA was isolated from the hepatic cells grown after successful transfection of the plasmids containing shRNA molecules against both target genes as per the manufacturer’s protocol using Trizol (Sigma). The RNA samples were treated with DNase I (Fermentas ...
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bioRxiv - Plant Biology 2023Quote: ... for detection of FLS2-BAK1 co-immunoprecipitation or anti-rabbit IgG (whole molecule)– HRP (A0545, Sigma, dilution 1:10000) for all other western blots.
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bioRxiv - Immunology 2023Quote: ... FITC-dextran is a nonabsorbable molecule with average molecular weight of 3000-5000 (Sigma-Aldrich, St. Louis, MO, USA). The FITC-dextran was diluted to 100 mg/mL with PBS and mice received a gavage of 0.6 mg/g once after a 4-h fast the day before termination of treatment ...
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iDePP: a genetically encoded system for the inducible depletion of PI(4,5)P2 in Arabidopsis thalianabioRxiv - Plant Biology 2020Quote: ... 2 ml of 50 % HIS-Select® Cobalt affinity resin (Sigma) was washed with milli-Q water and then with equilibration buffer (20 mM imidazole ...
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bioRxiv - Evolutionary Biology 2020Quote: ... complete His-Tag Purification Resin (NiNTA) were purchased from Sigma Aldrich. Protein inhibitor cocktail (PIC ...
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bioRxiv - Biochemistry 2021Quote: ... anti-TtSmtB antibodies (GeneCust™) and His-tag antibodies (Sigma-Aldrich). His-tag removal from recombinant TtSmtB was performed as previously described (22).
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bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified lysate was applied to Ni-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and then eluted in batches with buffer containing increasing amounts of imidazole (up to 800 mM) ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified lysate was applied to Ni-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and then eluted in batches with buffer containing increasing amounts of imidazole (up to 300 mM) ...
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bioRxiv - Microbiology 2021Quote: ... and a combination of α-6×His antibody (Sigma Aldrich, H1029) and HisProbe-HRP (Thermo ...
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bioRxiv - Cell Biology 2021Quote: ... His-tag was removed using an Enterokinase Cleavage Capture kit (Novagen), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... the proteins were purified using His-Select Nickel agarose resin (Sigma) and anion exchange chromatography (Source Q ...
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bioRxiv - Microbiology 2021Quote: ... His-tag removal was carried out by overnight HRV-3C (Sigma) digestion at 4°C and the cleaved protein was then purified on Ni-NTA before being subjected to SEC on Superose 6 10/300 column (Cytiva ...
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bioRxiv - Biochemistry 2019Quote: ... The His-tag was cleaved by digestion with thrombin (Sigma-Aldrich) in the presence of 2 mM CaCl2 at 37°C for 2 hours ...
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bioRxiv - Microbiology 2021Quote: ... Ni2+-NTA His-bind slurry was obtained from Novagen (Darmstadt, Germany). Rhodamine B isothiocyanate-Dextran (RITC-Dextran ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins were purified using Ni2+-containing His-Bind columns (Novagen). Purified proteins were desalted and concentrated by ultracentrifugation (Amicon ...
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bioRxiv - Cell Biology 2020Quote: ... followed by immunoblotting with HRP-conjugated anti-His antibody (Sigma-Aldrich) by using SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... –Ade (20 ug/mL L-His HCl monohydrate (A-9126, Sigma) added to –Trp ...
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bioRxiv - Immunology 2021Quote: ... either supplemented with 10% HI dialysed and filtered FCS (Sigma-Aldrich) 0.1M HEPES ...
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bioRxiv - Microbiology 2021Quote: ... probed using 1:500 monoclonal anti-His HRP-conjugated antibodies (Sigma) and imaged using a ChemiDoc MP (Bio-Rad) ...
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bioRxiv - Genetics 2022Quote: ... the ORF of eggpl was inserted into vector piztv5-His (Novagen) in our laborary to overexpress eggpl ...
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bioRxiv - Plant Biology 2022Quote: ... loaded onto His-Bind® Resin columns (EMD Millipore; Merck KGaA), and the recombinant ZmEREB57 protein was eluted with elution buffer (0.5 M NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... or anti-His primary antibodies (dilution 1:3000, #H1029 Sigma Aldrich).
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bioRxiv - Biochemistry 2023Quote: ... and supernatant was bound to Ni-NTA His-Bind Resin (Novagen) for 1hr at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was loaded onto Ni-resin (HIS-Select, Sigma-Aldrich) and washed with buffer 1 (50 mM Hepes-KOH pH7.6 ...
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bioRxiv - Genetics 2019Quote: Cells from the C2C12 mouse myoblast cell line (Sigma-Aldrich, St Louis, MO) were cultured in growth medium consisting of ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were stained with mouse-anti-p65 (Sigma) for 1 hour followed by incubation with goat anti-mouse Alexa Fluor 488 secondary IgG antibody (Life Technologies) ...
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bioRxiv - Biophysics 2021Quote: ... Mouse Do-11-10 thyoma cells (Sigma, Cat#85082301) were maintained in ImmunoCult™-XF T Cell Expansion Medium (STEMCELL™ Technologies Inc. ...
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bioRxiv - Neuroscience 2022Quote: ... cells were incubated with anti-HA (mouse; Sigma-Aldrich) antibody that was pre-clustered with secondary antibody-Cy5 for 40 minutes (ratio 1:7.5 for primary:secondary antibody) ...
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bioRxiv - Cell Biology 2021Quote: ... Male (xy) mouse C57BL/6N ES cells (Millipore, SCC050) (S1 Fig ...
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bioRxiv - Immunology 2020Quote: Cells were blocked with mouse serum IgG (Sigma, 15381) for 10 min at room temperature and then incubated with specific antibodies ...
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bioRxiv - Cell Biology 2023Quote: Mouse ES cells were maintained on 0.1% gelatin (Sigma) coated plates in mouse ES cell medium (DMEM ...
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bioRxiv - Developmental Biology 2021Quote: ... Flag-tagged proteins were purified by immunoprecipitation with anti-Flag M2 antibody (Sigma) and Protein G Mag Sepharose (GE healthcare) ...
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bioRxiv - Developmental Biology 2020Quote: ... Proteins associated with 3xFLAG-tagged MBD3 were purified using anti-FLAG sepharose (Sigma) and processed for mass spectrometry as described (Smits et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... FLAG-tagged fusion proteins were immunoprecipitated using Anti-FLAG M2 magnetic beads (Sigma) according to manufacturer’s instructions ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... FLAG and Myc tagged-proteins were detected with mAb-FLAG M2 (Sigma-Aldrich) and mAb-Myc 1-9E10.2 (ATCC) ...