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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for Mouse VPS10 domain containing receptor SorCS1 SORCS1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... follicle stimulating hormone (FSH)/luteinizing hormone (LH) (ELISA, EMD Millipore), AMH (ELISA ...
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bioRxiv - Immunology 2021Quote: ... Elisa plates were coated with 100ug/ml of OVA (Sigma) prepared in a coating buffer (Na2CO3 ...
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bioRxiv - Molecular Biology 2019Quote: ... ELISA 96-well plates (Nunc-Immuno™,Sigma-Aldrich®) were coated with 200 ng of purified anti-NS1 monoclonal antibody in carbonate buffer (0.05 M ...
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bioRxiv - Immunology 2022Quote: ... 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA (Sigma) was added and after 20 minutes the reaction was terminated by adding Stop Reagent for TMB Substrate (Sigma) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Pramlintide was quantified by ELISA (Merck Millipore, cat # EZHA-52) according to manufacturer instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Genomic DNA for genotyping was isolated from mouse tail snips with lysis buffer containing Proteinase K (Sigma-Aldrich, P2308). qPCR was performed using GoTaq qPCR Master Mix (Promega ...
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bioRxiv - Neuroscience 2020Quote: Mice were transferred onto a diet of crushed mouse food (Barrestock) containing 0.2% (w/w) cuprizone powder (C9012, Sigma), which was refreshed every 2 days for 5 weeks ...
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bioRxiv - Neuroscience 2020Quote: Sox14GFP/+ mouse brain samples (n=3) were embedded in a 4.5% oxidised-agarose solution containing agarose (type 1; Sigma), 10 mM NaIO4 (Sigma ...
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bioRxiv - Cell Biology 2022Quote: E14 mouse ES cells (male, RRID: CVCL_C320) were cultured in the serum/LIF medium containing Glasgow Minimum Essential Media (Sigma), 10% heat-inactivated FBS (HyClone) ...
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bioRxiv - Neuroscience 2020Quote: Whole-cell lysates were prepared from whole mouse brains using CST buffer (Cell Signal Technology) containing protease inhibitor (Sigma), and were rotated at 4°C for 30 minutes before DNA was pelleted ...
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bioRxiv - Immunology 2019Quote: ... resuspended in RPMI-10% FBS containing 40ng/mL CTLA4-Ig (CD152/Fc chimera, non-cytolytic, from mouse, Sigma C4358), plated in 24-well culture plates at a density of 2 x 106 cells/well and cultured for an additional 24 hours (BMDCCTLA4-Ig) ...
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bioRxiv - Neuroscience 2023Quote: ... Each mouse was treated with 500μg 6-OHDA (dissolved in 200 μl sterile PBS containing 0.01% ascorbic acid; Sigma-Aldrich), administered by subcutaneous injection daily for 2 days ...
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bioRxiv - Neuroscience 2024Quote: Sox14gfp/+ and Sox14gfp/gfp mouse brain samples were embedded in a 4.5% oxidised-agarose solution containing agarose (type 1; Sigma), 10 mM NaIO4 (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue was incubated in a primary antibody solution containing 1:250 mouse anti-NeuN (Millipore Sigma; MA, USA) or 1:200 goat anti-DCX (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2021Quote: Quantification of IL-6 in media was assessed via Human IL-6 ELISA kit according to manufacturer instructions (Sigma Aldrich; St. Louis, MO).
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bioRxiv - Immunology 2022Quote: Concentrations of human serum albumin in human plasma and bronchoalveloar lavage fluid were measured using an ELISA kit from Sigma Aldrich (Cat. #RAB0603) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: IDUA enzyme expression was measured fluorometrically in a 96-well plate using a Human IDUA ELISA kit (Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer’s sandwich assay protocol ...
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bioRxiv - Immunology 2022Quote: ... the DuolinkTM InSitu Orange Starter Kit Mouse/Rabbit (DUO92102) from Sigma Aldrich was used ...
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bioRxiv - Developmental Biology 2019Quote: ... Genotyping was performed using the KAPA HotStart Mouse Genotyping Kit (Sigma, KK7352) using GFP primers ...
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bioRxiv - Cancer Biology 2021Quote: ... The rat/mouse insulin kit (EZRMI-13K) was purchased from EMD Millipore Corporation ...
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bioRxiv - Developmental Biology 2022Quote: ... Genotyping was performed using the KAPA HotStart Mouse Genotyping Kit (Sigma, KK7352) using primers listed in Supplementary Table 2 ...
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bioRxiv - Cancer Biology 2020Quote: The DuoLink® In Situ Red Starter Kit Mouse/Rabbit (Sigma-Aldrich) was used to detect proximity between FXR1 protein and cMYC mRNA according to manufacturer protocol ...
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bioRxiv - Microbiology 2020Quote: ... using Milliplex Mouse Cytokine/Chemokine MAGNETIC BEAD Premixed 25 Plex Kit (Millipore), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... and mouse insulin RIA kit (Cat#SRI-13K, Millipore, Billerica, Massachusetts, USA) was used to measure plasma insulin ...
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bioRxiv - Physiology 2020Quote: ... we performed PLAs using the Duolink® Starter Kit Mouse/Rabbit (Sigma) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: The DNA construct coding for the motor domain of human kinesin-5 (Eg5; residues 1–368) was cloned into a modified pET16b (Novagen) vector for bacterial expression to achieve an N-terminally 6x His tag and TEV cleavage sequence ...
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bioRxiv - Biochemistry 2020Quote: A synthetic DNA fragment encoding the MTase domain of Arabidopsis thaliana DRM2 (residues 270-626) was cloned into pRSFDuet-1 vector (Novagen), preceded by an N-terminal His6-SUMO tag ...
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bioRxiv - Biochemistry 2022Quote: ... The protein complexes were eluted by on-column cleavage of MyoB neck domain using TEV and subsequently concentrated using a centrifugal concentrator (Millipore). The samples were analyzed using SDS-PAGE ...
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bioRxiv - Microbiology 2020Quote: ... Purified proteins were diluted to a final concentration of 50 mM NaCl and then concentrated on Amicon centrifugal filters 30K for FHL and 10K for LIM domains (Millipore). The proteins’ purities and identities were confirmed by SDS-PAGE and mass spectrometry ...
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bioRxiv - Biochemistry 2021Quote: ... and 25 ng/μl E3 (HUWE1, catalytic domain, aa 3228-4374/1147 aa) were incubated with 0.3 μg/μl Ub (Sigma-Aldrich) and 11 ng/μl H1.2/H1.2 KxAc in 2 mM ATP ...
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bioRxiv - Biochemistry 2022Quote: The Sso2 sequence excluding only its C-terminal transmembrane domain (aa1-270) and the Sec3 PH domain sequence (aa75-260) were both sub-cloned into the pET-15b vector (Novagen) between the NdeI and BamHI sites ...
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bioRxiv - Neuroscience 2022Quote: ... we added polyclonal antibodies against the extracellular domain of TrkB (1:50 rabbit anti-TrkB, Millipore, cat no. AB9872, RRID:AB_2236301) together with 20 ng/mL BDNF for 2.5 hours ...
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bioRxiv - Biophysics 2020Quote: ... SEPT8G and SEPT11G) were performed by inserting the CDS for the corresponding G domains into the bicistronic expression vector pETDuet-1 (Novagen). The G domain of SEPT7 (SEPT7G ...
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bioRxiv - Biophysics 2020Quote: ... and 10 μg/ml anti-E-cadherin blocking antibody that targeted the extracellular domain of E-cadherin (Gumbiner, Stevenson and Grimaldi, 1988) (uvomorulin, monoclonal anti-E-cadherin antibody, Sigma); after incubation ...
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bioRxiv - Molecular Biology 2021Quote: ... and the activity of Rac1 and Cdc42 were assessed by affinity purification using magnetic beads coupled to the p21-binding domain of p21-activated protein kinase (PAK) (Millipore) that specifically binds the active (GTP-bound ...
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bioRxiv - Microbiology 2023Quote: ... the purified proteins and domains were pooled and concentrated to 0.2–1 ml in PBS buffer by Amicon Ultra-15 (Millipore, USA). A BCA Protein Assay Kit (Beytotime Bio Co Ltd. ...
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bioRxiv - Biochemistry 2022Quote: ... The YopH tyrosine phosphatase expression vector pESU009 was generated by cloning the YopH phosphatase domain (residues 177-468) into pACYCDuet-1 (Novagen) under transcriptional control of an attenuated tac promoter.37 All constructs were confirmed by DNA sequencing.
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bioRxiv - Molecular Biology 2024Quote: cDNAs of BTB domains were PCR-amplified using corresponding primers (Supplementary Table S1) and cloned into modified pET32a(+) vector (Novagen) encoding TEV protease cleavage site after 6xHis-tag and Thioredoxin ...
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bioRxiv - Developmental Biology 2021Quote: ... 1.8 μg/mL VEGF Receptor Tyrosine Kinase Inhibitor II (VEGFR-TKI II; Calbiochem/EMD Millipore #676481-5MG) dissolved in DMSO was used to disrupt blood vessels57 and 100 μM γ-secretase inhibitor IX (DAPT ...
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bioRxiv - Developmental Biology 2021Quote: ... VEGF Receptor Tyrosine Kinase Inhibitor II (VEGFR-TKI II; Calbiochem/EMD Millipore #676481-5MG, 1.8 μg/mL) or equivalent amount of DMSO vehicle was added to media.
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bioRxiv - Neuroscience 2021Quote: ... we now infused .22ul of either saline or 2mM muscimol (a GABA-A receptor agonist; Sigma Aldrich). Our infusion targets differed by experiment ...
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bioRxiv - Biophysics 2021Quote: ... The receptor was concentrated to 20–30 mg/ml with a 100 kDa cut-off concentrator (Millipore). Protein purity and mono-dispersity were examined by Nu-PAGE and analytical size-exclusion chromatography.
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bioRxiv - Neuroscience 2021Quote: Primary antibodies and dilutions were: Anti-Purinergic Receptor P2RY14 rabbit (extracellular) (1:200, Sigma Aldrich, Cat#: P0119); GPR105 Polyclonal antibody rabbit (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated with an antibody against the M2 muscarinic acetylcholine receptor (1:500 in PBS, MAB367, Millipore) for at least 24 hours ...
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bioRxiv - Immunology 2022Quote: ... sera were heat-inactivated at 56 °C for 30 minutes and treated with receptor destroying enzyme (Sigma) in a 1:4 mixture (5x dilution of sera) ...
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bioRxiv - Neuroscience 2022Quote: ... AMPA-receptor-mediated excitatory postsynaptic currents (EPSCs) were recorded in presence of picrotoxin (100 μM, Sigma Aldrich) to block GABAARs ...
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bioRxiv - Bioengineering 2023Quote: ... The stimulation of ACh to muscarinic receptors was blocked by addition of 10 nM Atropine (Sigma Aldrich). In the presence of Atropine ...
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bioRxiv - Neuroscience 2024Quote: ... Aladdin) or β receptor agonist isoprenaline (2 mg/mL, 4 mg/mL, n = 7, I5627, Sigma-Aldrich) or β receptor antagonist propranolol (2·5 mg/mL ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Sections containing SN and VTA were incubated with antibodies directed against tyrosine hydroxylase (mouse monoclonal, 1:1000, Merck-Millipore MAB318) and mCherry (rabbit polyclonal ...
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bioRxiv - Cell Biology 2021Quote: ... The blot was blocked in 5% Milk in tris-buffered saline with 0.1% Tween 20 (TBST) and then incubated in 5% Milk TBST containing mouse anti-DPYD (Sigma, WH0001806M1) and rabbit anti-β-actin (Cell Signaling ...