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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for Glioma pathogenesis related protein 1 GliPR1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: HEK293 cells stably expressing FLAG-GPR65 were plated to poly-d-lysine (Sigma Aldrich) coated 12 mm glass coverslips (Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 48-well cell cultures were supplemented with 100 µM guanosine (Sigma Aldrich #G6264). DMSO and 100 µM adenosine (Sigma Aldrich #A9251 ...
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bioRxiv - Microbiology 2019Quote: ... PTP-tagged proteins were detected by incubating with anti-protein A serum (1:3000, 1 h) (Sigma, P3775) followed by Alexa Fluor® 594 goat anti-rabbit (1:250 ...
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bioRxiv - Developmental Biology 2021Quote: ... were soaked overnight with human SDF-1 3.3 μg ml−1 (Sigma-Aldrich, S1577) at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1; Sigma-Aldrich, USA), and 20 ng/ mL human bFGF (Life Technologies)] with 10 μM Rho kinase inhibitor Y-27632 (Wako ...
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bioRxiv - Neuroscience 2020Quote: ... and 1% protein phosphatase inhibitor cocktail (Sigma). Lysed tissues were incubated at 4°C for 20 min and centrifuged at 15,000 rpm for 15 min ...
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bioRxiv - Genetics 2021Quote: ... PolyUbiquitinated proteins (1/1000, Sigma 04-262), p62 (1/1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Detection of proteins was performed by using primary rabbit anti-human hepsin antibody (Sigma Aldrich, Steinheim, Germany), pAKT (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 10 ng/ml of the recombinant human TNFα protein (Merck-Millipore, Cat# GF023) and the outcome was assessed at RNA or protein levels.
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bioRxiv - Biochemistry 2023Quote: The in vitro phosphorylation is conducted using the human Src-GST protein (Sigma-Aldrich, Catalogue number: S1076) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated with Protein A Agarose/Salmon Sperm DNA and 5 μg human IgG (Sigma, Cat# I4506) or PPAR-γ Antibody (E-8 ...
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bioRxiv - Biophysics 2022Quote: ... 5 μL of the dye solution was added to 1 mL 5 mg/mL HI (91077C, Sigma-Aldrich, 95%) monomer solution ...
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bioRxiv - Biochemistry 2021Quote: ... and the lysate was incubated for 1 h with a previously equilibrated His-Select Nickel Affinity Gel (Sigma-Aldrich). Weakly-bound proteins were removed using buffer A (20 mM imidazole ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
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bioRxiv - Biophysics 2021Quote: ... The his-tag affinity purified stator was concentrated to 1 ml using a 100 K MWCO Amicon device (Millipore). The samples were subjected to size exclusion chromatography using Enrich SEC650 column (Bio rad ...
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bioRxiv - Plant Biology 2022Quote: ... The transformants were then spotted on SD (−Trp, −Leu, −His) selection media containing 1 mM 3-amino-1,2,4-triazole (Sigma, A8056). The positive interactors were then scored based on the stringency of the selection.
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bioRxiv - Immunology 2023Quote: ... and digested in 4 mL of RPMI-1640/5% HI-FBS containing 1 mg/mL collagenase D (Sigma-Aldrich) and 30 μg/mL DNase I (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... The 8 ml supernatant was applied to 1 ml packed volume of Ni-NTA His-Bind Resin (Millipore Sigma). Binding was performed with gentle mixing for 1 hour at 4°C and loaded onto a Poly Prep Chromatography Column (BioRad) ...
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bioRxiv - Biochemistry 2024Quote: ... with a TEV cleavage site and a second ribosomal binding and multiple cloning site (pABLO TEV) and His-tagged constructs were cloned into a modified form of pRSF_Duet-1 (Novagen) with a TEV cleavage site following the hexahistidine tag ...
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bioRxiv - Physiology 2020Quote: ... For insulin clearance during an insulin challenge, mice were given human insulin (1 U/kg, NovoRapid) or human C-peptide (50 µg/kg, Sigma) by intraperitoneal injection and blood samples were collected by tail vein sampling at 0 ...
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bioRxiv - Microbiology 2020Quote: ... membranes were probed with 1 in 10,000 dilution of human anti-SIC followed by 1 in 50,000 dilution of HRP-conjugated goat anti-human IgG (Sigma-Aldrich). To directly compare detection of SIC fragments by purified human and rabbit anti-SIC antibodies ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibody was diluted in 1% human AB serum (Sigma-Aldrich) in PBS at the concentrations in Table 2 and incubated overnight ...
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bioRxiv - Immunology 2021Quote: ... mouse anti-human/mouse SMA-Cy3 (1:200, #C6198, Sigma Aldrich), rat anti-mouse CD35 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... or 1:5000 HRP-conjugated anti-human IgM antibody (Sigma #AP114P) was added to each well ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies against mouse or human laminin (1:300 rabbit, Sigma), mouse collagen IV (EMD Millipore) ...
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bioRxiv - Systems Biology 2020Quote: ... containing 1% non-autologous human plasma (Sigma-Aldrich, St Louis, USA), 2 mM L-glutamine (Lonza) ...
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bioRxiv - Biochemistry 2020Quote: Alpha-1-acid glycoprotein (AGP) purified from human serum (Sigma #G9885) and A/Philippines/2/1982 (Phil82 ...
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bioRxiv - Neuroscience 2020Quote: ... and mouse anti human Nestin (Millipore, product no: MAB5326, (1:200)) ...
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bioRxiv - Biochemistry 2021Quote: ... and CF-640R conjugated goat anti-human (1:1000; Sigma-Aldrich) secondary antibodies were used to probe for phospho-Histone H2A.X and β-Actin respectively ...
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bioRxiv - Cancer Biology 2019Quote: ... followed by incubation with rabbit anti-human TCF21 (Sigma, 1:100), mouse-anti-human-CD49e (BD Biosciences ...
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bioRxiv - Cancer Biology 2019Quote: ... Human head and neck carcinoma line UM-SCC-1 (EMD Millipore) was cultured in DMEM containing 20% FBS ...
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bioRxiv - Genomics 2021Quote: ... human Cot-1 placental DNA from Sigma-Aldrich (Oakville, ON, Canada), and target-specific biotin-labeled probes in hybridization solution ...
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bioRxiv - Biochemistry 2020Quote: ... HRP-conjugated anti-human Fc antibody (1:10000 dilution, Sigma Aldrich) was used ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1:2000 HRP-labelled rabbit anti-human IgG (Sigma-Aldrich, #A8792) in blocking solution was added ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were labeled with goat anti-human ChAT (1/500; Millipore) or goat anti-IBA1 (Abcam) ...
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bioRxiv - Immunology 2021Quote: ... 1:3000 dilution of anti-human IgA (α-chain-specific) (Sigma) or 1:3000 dilution of anti-human IgM (μ-chain-specific ...
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bioRxiv - Genomics 2021Quote: ... Monoclonal mouse anti-human TRA-1-60 Antibody (Millipore MAB4360C3,1:100), Monoclonal mouse Anti-Stage-Specific Embryonic Antigen-4 Antibody (Millipore ...
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bioRxiv - Pathology 2021Quote: ... rats were injected intraperitoneally with 1 U/kg human insulin (Sigma). Blood glucose from the tail vein was measured using a glucometer at the desired time (0 ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-human mitochondrial marker (1:500, Millipore, MAB1273 clone 1113), and sheep anti-eGFP (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... and centromeres using 1:500 human anti-centromere antibodies (ACA; Sigma) for 2 hours at room temperature ...
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bioRxiv - Immunology 2022Quote: ... HRP-conjugated anti-human pan immunoglobulin secondary antibody (Sigma; 1:10,000) was added (50mL per well ...
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bioRxiv - Immunology 2022Quote: ... and added by anti-human IgG-HRP (1:60000 dilution, Sigma) for 30 min incubation under 37°C ...
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bioRxiv - Immunology 2023Quote: ... anti-human/mouse ACTA2 Cy3 (Clone 1A4, Sigma C6198, 1:1000), anti-human CD31 A647 (Clone WM59 ...
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bioRxiv - Bioengineering 2023Quote: ... with 1% human platelet poor-derived serum (HS; Sigma-Aldrich, P2918), 20 ng/mL basic FGF (bFGF ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... human specific N terminal anti-collagen VII LH7.2 (1:250 Millipore), The fluorescence images were taken using the TCS SP5 confocal laser scanning microscope (Leica) ...
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bioRxiv - Neuroscience 2023Quote: ... and fibronectin from human plasma (Sigma-Aldrich F1056; 1 μg/ml). For some experiments ...
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bioRxiv - Cell Biology 2023Quote: ... containing 1% Human Platelet Poor Plasma-derived Serum (SH, Sigma, P2918), 20 ng/mL FGF2 and 10 uM All-trans Retinoic Acid (RA ...
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bioRxiv - Neuroscience 2023Quote: ... purified calpain-1 from human or porcine erythrocytes (8 µg, Millipore) or human or rat recombinant calpain-2 produced by bacterial expression as described by (Elce et al. ...
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bioRxiv - Bioengineering 2024Quote: ... anti-human nuclei (HuNu; 1:100; MAB4383, Merck Millipore, MA, USA), anti-Ku80 (1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... 1% PS) with freshly added 8 ng/mL human bFGF (Sigma). The medium was changed daily ...