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Citations for Millipore Sigma :
701 - 750 of 10000+ citations for Glioma pathogenesis related protein 1 GliPR1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Human DPP4 (Sigma) was used as a control in PBS at 50 ng/μL (0.585 μM) ...
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bioRxiv - Biophysics 2020Quote: ... We induce protein expression by adding 1 mM isopropyl β-d-1-thiogalactopyranoside (Sigma) when the cell density reaches an ODλ=600nm of 0.6 ~ 0.7 ...
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bioRxiv - Cancer Biology 2023Quote: ... The M-protein solutions were diluted 1:1 with 8 M Urea (Sigma Aldrich) in Tris.HCl (pH=7 ...
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bioRxiv - Microbiology 2019Quote: ... The washing step was repeated and 200 µl of a 1:5,000 dilution of the monoclonal Anti-His antibody (Sigma-Aldrich, St. Louis, MO) in PBS + 2% BSA was added to each well ...
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bioRxiv - Cell Biology 2019Quote: ... 20mM reduced glutathione) and analyzed by SDS-PAGE followed by Coomassie staining and immunoblotting with anti-His (dilution: 1:5000; Sigma-Aldrich, Steinheim, Germany), or anti-GFP-antibodies (dilution 1:1000 ...
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bioRxiv - Biophysics 2021Quote: ... Protein production was confirmed by SDS-PAGE and subsequent Western blotting analysis of a K30 sample after 24 h using an anti-His antibody from mouse (dilution 1:3000, Sigma-Aldrich, cat.no. H1029) and secondary anti-mouse IgG-HRP antibody produced in goat (dilution 1:20,000 ...
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bioRxiv - Plant Biology 2022Quote: ... in the same buffer containing 1/5000 of primary antibody raised against poly-His tag directly coupled with peroxidase (Sigma, catalog no. A7058). After washing 3x by PBS 1x ...
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bioRxiv - Molecular Biology 2020Quote: ... a single fluorescent cell was sorted per well of a fibronectin-coated (10 μg/mL Human Plasma Fibronectin Purified Protein (Sigma Aldrich) in 1x PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Recombinant Human Protein #PHG0264), 10 ng/ml epithelial growth factor (EGF, Recombinant Human Protein #PHG0311) and 10 µM ROCK Inhibitor Y-27632 (Sigma, #Y0503) were added to the media freshly before use ...
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bioRxiv - Biochemistry 2023Quote: ... No. H7379) and human hemoglobin A0 (HbA0) (with 5% (w/w) methemoglobin) (Cat. No. H0267) (all protein samples from Sigma, USA). Tetrahydrofuran (THF ...
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bioRxiv - Neuroscience 2021Quote: The transfected HEK293 cells were seeded on glass coverslips (Matsunami) coated with poly-L-lysine solution (Sigma-Aldrich) then incubated for 4–10 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Neuro-2a (N2a) and HEK293 cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) high glucose (Sigma-Aldrich) supplemented with 10% (v/v ...
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bioRxiv - Developmental Biology 2019Quote: ... HEK293 cell viability after iOn plasmids transfection was assessed by dye exclusion with Trypan blue solution (0.4%, Sigma). FP expression was either assayed by flow cytometry ...
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bioRxiv - Cell Biology 2022Quote: HEK293-FLP cells stably expressing UBC:DOR-APEX2 were plated onto poly-L-lysine (Sigma Aldrich, Cat # P8920-100ML) coated coverslips in wells of a 24-well plates ...
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bioRxiv - Microbiology 2023Quote: HeLa cells and HEK293 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% foetal bovine serum (Sigma) at 37°C in 5% (V/V ...
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bioRxiv - Cancer Biology 2021Quote: ... at 1:56.6 dilution and Protein-A-agarose beads (Millipore, cat# IP02 ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1 mM protein phosphatase Inhibitor Cocktail I (Sigma Aldrich). Protein lysates were mixed with 5X SDS sample buffer and boiled then resolved on SDS-PAGE gels ...
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bioRxiv - Genetics 2021Quote: ... 1:250 rat anti-myelin basin protein (MBP) (Millipore MAB386); secondary antibodies were Alexa Fluor® conjugates from Life Technologies and used at 1:500 ...
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bioRxiv - Developmental Biology 2019Quote: ... rabbit anti-Cytoplasmic interacting protein 1 (Millipore-Sigma, Cat # Ab6046), rabbit anti-β-Catenin polyclonal antibody (ThermoFisher ...
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bioRxiv - Developmental Biology 2019Quote: ... rabbit anti-Cytoplasmic interacting protein 1 (Millipore-Sigma, Cat # Ab6046), rabbit anti-β-Catenin polyclonal antibody (ThermoFisher ...
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bioRxiv - Neuroscience 2019Quote: ... Proteins were detected with antibodies against GFP (Sigma, 1:1000) and FMR polyclonal antibodies (1:1000) ...
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bioRxiv - Biochemistry 2019Quote: ... and supplemented with SyproOrange Protein Gel Stain 1:500 (Sigma). After incubation on ice for 1.5 h ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg of total protein and anti-GFP (Sigma SAB4301138) at 1:500 dilution was used ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-microtubule-associated protein 2 (Map2, 1:250, Sigma), rabbit anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Molecular Biology 2020Quote: ... containing protein phosphatase inhibitor cocktail 1 and 2 (Sigma-Aldrich) and protease inhibitors (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant protein was induced with 1 mM IPTG (Sigma – Aldrich) and the culture was transferred to 25°C and 250 rpm for 5 hours ...
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bioRxiv - Developmental Biology 2022Quote: ... proteins were precipitated using 1 mg of gamma-globulins (Sigma) and 2 ml 20% TCA followed by centrifugation at 2,000 r.p.m ...
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bioRxiv - Plant Biology 2022Quote: ... Six ml of protein extraction buffer (1 tablet of Sigma Complete™ mini protease inhibitor ...
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bioRxiv - Cell Biology 2020Quote: ... and RSV F protein (1:500 dilution, 488 conjugated; Millipore). Cultures were mounted using DAPI mounting medium (Vectashield ...
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bioRxiv - Physiology 2021Quote: ... followed by peroxidase-conjugated Protein G (Sigma P8170; 1:130’000) or peroxidase-conjugated anti-mouse IgG (Fab Specific ...
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bioRxiv - Neuroscience 2020Quote: ... glial fibrillary acidic protein (GFAP, 1:1000, #SAB4100002, Sigma-Aldrich), NeuN (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... Sftpc (1:100, anti Pro Surfactant protein, rabbit, AB3786 Millipore), Ki67 (1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies against pan-talin protein (1:1000, Sigma-Aldrich) and actin control (1:2000 ...
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bioRxiv - Developmental Biology 2022Quote: ... rabbit anti-pro-surfactant protein C (Sigma, Ab3786; 1:2000), rabbit anti-TGFβ2 (Proteintech ...
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bioRxiv - Molecular Biology 2019Quote: ... 4-HNE protein adducts were detected by an anti 4-HNE protein adduct antibody (1:2000, AB5605, Millipore) in Tris-buffer saline with 5% skim milk ...
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bioRxiv - Molecular Biology 2024Quote: ... insulin-like growth factor 1 (IGF-1; 100 ng/ml; recombinant Human LONG R3 IGF-1, Sigma-Aldrich, Cat# I1271, MO, USA), rapamycin (10 ng/ml ...
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bioRxiv - Biochemistry 2020Quote: ... full length human PCNA (accession no. NM182649) was cloned into pETDuet-1 MCS1 (Novagen) Amp+ to obtain 6× His N-terminally tagged protein ...
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bioRxiv - Immunology 2019Quote: ... incubated with alkaline phosphatase-conjugated goat anti-human IgG (1 μg/mL, Sigma-Aldrich) for 1 hr at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... Human cells were detected using directly Cy3-conjugated anti-HNA (Millipore, MAB1281C3, 1:100). After washing in TBS ...
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bioRxiv - Bioengineering 2020Quote: ... anti-human mitochondria antibody Alexa Fluor® 488 conjugate (MAB1273A4, 1:100, EMD Millipore), and DAPI.
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bioRxiv - Biochemistry 2020Quote: ... then resuspended in 1:500 anti-human IgG antibody conjugated to Cy3 (Sigma #C2571). Fluorescence staining was measured by a BD Fortessa flow cytometer.
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bioRxiv - Microbiology 2020Quote: ... 5 μg human insulin mL-1 and 5×10-7 M hydrocortisone hemisuccinate (Sigma). Cells were seeded and expanded for two weeks and subsequently differentiated for another two weeks in the presence of 1.8% DMSO61 (dHepaRG).
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bioRxiv - Microbiology 2020Quote: ... 5 μg human insulin mL−1 and 5×10−7 M hydrocortisone hemisuccinate (Sigma). As a control we generated HepaRG cells expressing an inactive HBx null mutant (HepaRG-HBxSTOP ...
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bioRxiv - Neuroscience 2022Quote: ... The following synaptic antibodies were used: Rabbit anti-human SYNAPSIN1 (1:1000, Millipore, AB1543), Chicken anti-human MAP2 (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... 50,000 human ECs (RBPJ knock-out or shRNA for CXCL2 (pLKO.1; Sigma Aldrich) and respective controls ...
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bioRxiv - Neuroscience 2022Quote: ... before blotting with the primary antibodies: Rabbit anti-human SYNAPSIN1 (1:1000, Millipore, AB1543) and Mouse anti-human GAPDH (1:1000 ...
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bioRxiv - Cancer Biology 2019Quote: ... Mouse monoclonal anti-human SHC4 (clone 2F5, dilution 1:300) was from Sigma-Aldrich. Rabbit polyclonal anti-human CHORDC1 (NBP1-78304 ...
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bioRxiv - Bioengineering 2021Quote: ... and 25 μg mL−1 fibronectin from human plasma (Cat. no. F1141, Sigma-Aldrich). Cells have previously been characterized for expression of tight junction proteins ...
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bioRxiv - Cell Biology 2020Quote: ... Immunofluorescence in human cells was conducted using antibodies against mouse □-tubulin (Sigma, 1:1000), mouse CENP-E (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... 5 μg mL−1 hemin and 10% (vol:vol) heat-inactivated human AB serum (Sigma). Cultures were sampled daily ...