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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for 7 Chloro 1 3 dihydro imidazo 4 5 c pyridin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... α-tubulin (clone B-5-1-2) (Sigma-Aldrich) and His-tag (Invitrogen ...
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bioRxiv - Neuroscience 2019Quote: ... 3 mM 5-phosphoribosyl 1-pyrophosphate (PRPP Sigma-Aldrich P8296), 2 mM ATP ...
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bioRxiv - Genomics 2021Quote: ... 5% normal donkey serum in PBS for 1 hour and incubated at 4°C overnight with primary antibody (EMD Millipore, cat. Ab3786, 1:300), then at room temperature for 1 hour with Alexa Fluor 594-conjugated secondary antibody (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were incubated with rabbit UPF1 antibody (CST #9435) antibody used at 1:1000 dilution in 5% BSA 1X TBST overnight at 4°C or mouse Tubulin (Sigma T9206) used at 1:2000 dilution in 5% milk 1X TBST for 1 hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... The fractions containing the complexes were incubated for 1 hr at 4°C with 10 mM N-hydroxysulfosuccinimide and 5 mM EDC (Sigma-Aldrich) following the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2021Quote: ... then incubated with primary antibodies diluted in 5% milk overnight at 4°C (anti-ATG13 (SAB4200135, 1:100, Sigma, MI, USA); anti-GAPDH (10494-1-AP ...
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bioRxiv - Molecular Biology 2023Quote: ... Half of the beads were then incubated for 1 h at 4°C in 50 µl of 5 mM free CoA (C4282, Sigma-Aldrich) (dissolved in H2O) ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 hour and incubated with the following primary antibodies diluted in 5% milk with TBST overnight at 4°C: Anti-β-actin (1:5000, A5441-.2ML Sigma); Anti-VAPA (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... The sections were stained with primary antibodies diluted in 5% v/v horse serum over night at 4°C: mouse anti- glucagon antibody (1:1000, Sigma-Aldrich), guinea pig anti-insulin (1:500 ...
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bioRxiv - Bioengineering 2021Quote: ... 5-norbornene-2-carboxylic acid (1.56 ml, 12.8 mmol, 4 eq.; Sigma-Aldrich) was added to the solution and the reaction was stirred overnight at room temperature (RT ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-5 µm slices were individually stained with Hematoxylin (Sigma #517-28-2), Picrosirius Red (Abcam #ab150681) ...
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bioRxiv - Microbiology 2024Quote: Sulfamethoxazole (IUPAC: 4-Amino-N-(5-methylisoxazol-3-yl)-benzenesulfonamide) was purchased from Sigma-Aldrich, USA ...
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bioRxiv - Neuroscience 2020Quote: ... 2-(5-Benzo [1,3] dioxol-5-yl-2-tert-butyl-3HImidazol-4-yl)-6-methylpyridine hydrochloride hydrate (SB505124) (Sigma-Aldrich; St. Louis, MO) and implanted subdermally ...
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bioRxiv - Bioengineering 2022Quote: ... with a 3:1 M ratio of 5-norbornene-2-carboxylic acid (mixture of endo and exo isomers; Millipore-Sigma) to HA-TBA repeat units ...
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bioRxiv - Biophysics 2023Quote: ... and ultrapure H2O and finally incubated in a solution of poly-T ssDNA (100 nM) in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (10 mM HEPES, NaCl 50 mM, pH 7, Sigma-Aldrich, St Louis, MO, USA) for 1 h at approx ...
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bioRxiv - Biochemistry 2019Quote: ... ChK2 inhibition was carried out by using 2-(4-(4-Chlorophenoxy) phenyl)-1H-benzimidazole-5-carboxamide hydrate (Sigma) at 0.2 mM concentration.
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bioRxiv - Bioengineering 2023Quote: ... Membranes were probed overnight at 4°C with anti-HA antibody (MBL, M180-3) and anti-glyceraldehyde-3-phosphate dehydrogenase antibody (Sigma-Aldrich, G8795) or with anti-dystrophin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... with secondary antibody in PBST containing goat serum, washed (3 × 5 min), stained (10 min, 20°C) with DAPI (1 µg/ml in PBS; Sigma, Cat# D9542) and washed (5 min ...
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bioRxiv - Physiology 2021Quote: ... were incubated at 4 °C overnight with 5 μg/mL anti-FLAG (Sigma-Aldrich, F1804) in 200 mM NaHCO3 buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... were incubated for 6 hours at 4°C with 5 μg H3K27me3 (07-449, Millipore), 5ug H2AK119ub (8240 ...
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bioRxiv - Developmental Biology 2019Quote: ... All animals were incubated at 28.5 °C for 5 h before treatment with 1-phenyl-2-thiourea (PTU) (Sigma Aldrich) to prevent pigment formation ...
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bioRxiv - Immunology 2019Quote: ... and incubated at 37°C for 2 min before addition of 5 ml of serum-free L-15 containing 1 μg/ml DNase1 (Sigma) for 5 min at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated (1 h, 50°C) with 2 µL 10% SDS and 5 µL proteinase K (10 mg/mL, Sigma). Chromatin was recovered by phenol/chloroform extraction and resuspended in 30 µL TE (1 mM tris-HCl pH8.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was incubated (1 h, 50°C) with 2 µL 10% SDS and 5 µL proteinase K (10 mg/mL, Sigma). Chromatin was recovered by phenol/chloroform extraction and resuspended in 30 µL TE (1 mM tris-HCl pH8.0 ...
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bioRxiv - Bioengineering 2023Quote: The mice were anaesthetized with isoflurane (induction 5%, maintenance 2−3%; Sigma-Aldrich, USA) and secured in a stereotaxic instrument (RWD ...
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bioRxiv - Microbiology 2023Quote: ... 3-ethoxy-4-hydroxybenzaldehyde (121-32-4, Sigma), N-(acid-PEG10)-N-bis(PEG10-azide ...
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bioRxiv - Cancer Biology 2019Quote: ... containing 5% nonfat dry milk and incubated overnight at 4°C with primary antibody diluted in PBST–5% nonfat dry milk or 5% bovine serum albumin (BSA, Sigma). Membranes were subsequently washed with PBST and incubated with the appropriate IRDye 680RD and IRDye 800CW secondary antibody (LI-COR Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... Free dyes and lipids were then purified away from labeled virus particles by ultracentrifugation for one hour at 4 °C at 40,000 rpm over a 6%-18% Optiprep (Sigma Aldrich) gradient ...
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bioRxiv - Microbiology 2020Quote: ... 2 and 3 emulsified 1:1 in Freund’s incomplete adjuvant (Sigma-Aldrich) on days 0 ...
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bioRxiv - Neuroscience 2021Quote: ... antisense: 5’-ACCGAUUGAAGUUAAUGUC-3’), Myef2 (sense: 5’-CUUUGGUGGAGUUGGCCGA-3’, antisense: 5’-UCGGCCAACUCCACCAAAG-3’) or siRNA universal negative control (SIC001, Sigma-Aldrich) were purchased from Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... 3-(N-Morpholino)propanesulfonic acid (MOPS) or 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPES) buffer (all purchased from Sigma-Aldrich, USA). Macrocolonies were excised and resuspended in 2 mL of sterile 1X PBS ...
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bioRxiv - Neuroscience 2019Quote: ... Specimens for histamine immunohistochemistry were prefixed overnight at 4°C in 4% N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDAC, Sigma–Aldrich E6383). Brains were washed six times for 15 minutes each in PBS and subsequently immersed in Poly-L-Lysin (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2019Quote: ... 15mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Sigma), 100U penicillin (Wako) ...
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bioRxiv - Neuroscience 2021Quote: ... 4’,6-diamidino-2-phenylindole (DAPI; 1:1000; Sigma) was added to the cell suspension ...
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bioRxiv - Neuroscience 2021Quote: ... 4’,6-diamidino-2-phenylindole (DAPI, 1:1000, Sigma) was used to show nuclei clear and dyed for 5 min ...
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bioRxiv - Developmental Biology 2023Quote: ... 4′,6′-diamidino-2-phenylindole (1:3000; Sigma-Aldrich) was used to counterstain sections and coverslips were mounted using Fluorogel l (Electron Microscopy Sciences ...
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bioRxiv - Neuroscience 2020Quote: ... to 30 µL CSF (pooled from 1 – 3 animals) and placing in 3 mm NMR tubes (Wilmad®, 7 inch; Sigma-Aldrich). Serum samples (samples pooled correspondingly to the CSF samples ...
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bioRxiv - Genetics 2019Quote: ... skimmed milk in PBS at the following concentrations for 2-20 h at 4°C (anti-Srs2, Santa Cruz sc-1191, 1:2000; anti PSTAIR, Sigma P6962, 1:2500). Membranes were washed 3x in PBS ...
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bioRxiv - Zoology 2023Quote: ... All wells were then washed with embryo media three times before being incubated on an orbital incubator shaker at 130 rpm and 28°C for 15-20 minutes in media containing 0.01% 2-[4-(dimethylamino) styryl]-1-ethylpyridinium iodide (DASPEI, Sigma Aldrich; cat. #3785-01-1) to selectively stain live neuromast hair cells ...
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bioRxiv - Plant Biology 2020Quote: ... samples were resuspended in Urea 7 M and Thiourea 2 M buffer and desalted on Amicon Ultra-0.5 3 kDa centrifugal filters (Merck Millipore, Germany). Filters were filled to maximum capacity with buffers and centrifuged at 15,000 g for 10 min at 20°C ...
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bioRxiv - Cell Biology 2021Quote: ... Skin was minced into small pieces (about 2-3 mm × 2-3 mm) and digested by incubation with 1 mg/mL collagenase V (Sigma) in PBS for one hour with shaking (250 rpm) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 2’,7’-dichlorofluorescein diacetate (DCFH-DA) (Sigma, D6883) was used to measure intracellular ROS levels ...
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bioRxiv - Cell Biology 2022Quote: ... 7 μl 2-mercaptoethanol (Sigma-Aldrich, M3148-25ML), and 7 μl GLOX solution.
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bioRxiv - Plant Biology 2019Quote: ... 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA, Sigma-Aldrich) was added at a final concentration of 10 μM and live cell imaging was performed using the Leica SP8-X microscope as described previously (Islam et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... the slides were then incubated overnight at 4°C with rabbit polyclonal to elafin in 2% BSA at 1:100 dilution (Sigma, HPA017737). The signal was detected using the rabbit horseradish peroxidase EnVision kit (DAKOCytomation ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes of primary motor neuron samples were incubated overnight at 4°C with primary antibodies for ERK1/2 (1:10000, Sigma Aldrich) and ATPB (1:1000 ...
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bioRxiv - Pathology 2022Quote: ... 1 part NTA), incubated (30min., RT) and centrifuged (3000g, 1h, 4°C) in an Ultra-2 Centrifugal Filter Unite (Millipore, #UFC203024). Filtered samples (100µL ...
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bioRxiv - Microbiology 2021Quote: ... 1 mL of these extracts were centrifuged at 14,000 xg for 1 min at 4°C with 2.5 μl 2% (v/v) Tween 20 (Sigma-Aldrich, Darmstadt, Germany) to promote the aggregation into a pellet ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were blocked with 5% BSA in Tween-TBS at room temperature for 2 h and incubated at 4 °C overnight with anti-His antibody (1:200; Sigma Aldrich) in blocking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... stained with 1’ ab for 12h at 4°C (EMD Millipore [ab144P ...