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Citations for Addgene :
151 - 200 of 960 citations for hsa mir 146a Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... the CHD7 ORF was PCR amplified from a plasmid (Addgene, 89460) using Phusion High-Fidelity Master Mix (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... the iTol2 flanked kanR-cryaa:sfGFP fragment was PCR amplified from Addgene #74153 (Fuentes et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR amplified 8xtetO-EF1a promoter-eGFP cassette from iDuet101a (Addgene, #17629) was cloned with Gibson assembly in frame with the SV40pA sequences that were PCR amplified from lentiCRIPSRv2 (Addgene ...
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bioRxiv - Genomics 2022Quote: ... and the previously digested hu6 PCR fragment (from pMJ117 Addgene 85997) with appropriate overhangs was inserted via T4 ligation ...
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bioRxiv - Synthetic Biology 2023Quote: ... The following expression vectors were amplified by PCR: RAD51 (Addgene #41815), CtIP (Addgene #109403) ...
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bioRxiv - Cell Biology 2023Quote: ... APEX2 was obtained by PCR from APEX2-csChBP (no. 108876, Addgene) and inserted in pMK-289 (no ...
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bioRxiv - Neuroscience 2023Quote: ... mScarlet was PCR amplified from the plasmid pmScarlet_C1 (Addgene plasmid #85042) while also adding a fyn myristolyation sequence (a membrane targeting sequence ...
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bioRxiv - Cell Biology 2023Quote: ... the CRY2 PHR domain was PCR amplified from pCRY2PHR-mCherryN1 (Addgene plasmid #26866 ...
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bioRxiv - Cell Biology 2023Quote: ... and mEmerald amplified by PCR from mEmerald-Sec61b-C1 (Addgene #90992), with a Gly-Gly-Gly-Ser linker placed either side of the HaloTag or mEmerald at Site 3 and Site 4 ...
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bioRxiv - Immunology 2024Quote: ... mCherry was amplified by PCR from the mCherry-Rab4a (Addgene #55125) plasmid and inserted with NEBuilder HiFi Assembly cloning kit (#E5520S ...
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bioRxiv - Systems Biology 2024Quote: ... was created by PCR cloning of 3xHA-MKK6-EE (Addgene, 47579) with BglII–SalI ends and inserting into pBABE puro (Addgene ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR amplicons were cloned into the pAGM9121 (Addgene plasmid #51833), pICH41308 (Addgene plasmid #47998 ...
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bioRxiv - Bioengineering 2024Quote: ... Firefly luciferase was PCR amplified from pCDNA-Luciferase (Addgene, cat # 18964) using primers introducing a 5’ KpnI site and a 3’ NheI site ...
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bioRxiv - Cancer Biology 2024Quote: ... HMGCR was PCR-amplified out of pCMV-SPORT6-hHMGCR1 (Addgene, 86085) using the forward primer 5’- GTATACTGGATCCGCCGCCACCATGTTGTCAAGACTT-3’ and reverse primer 5’-TCGGAGCTCGAGGTGGCTGTCTTCTTGGT-3’ ...
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bioRxiv - Cancer Biology 2021Quote: ... The PCR product was cloned into the pENTR4 no ccDB plasmid (Addgene #17424 ...
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bioRxiv - Cell Biology 2020Quote: ... full length P180 was PCR amplified from V5-GFP-P180 (Addgene #92150) and inserted in mCherry-N1 vector between XhoI and BamHI sites ...
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bioRxiv - Cell Biology 2019Quote: ... PCR products and a pCS2+8 vector (Gokirmak et al., 2012) (Addgene) were digested with XbaI and BamHI (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... the 24xMS2 stem loop sequence was purified from pCR-24xMS2L-stable (Addgene_31865) by restriction digest and the 128xMS2 from pMK123-128xMS2 (Tantale et al. ...
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bioRxiv - Cell Biology 2019Quote: Human STAMBPL1 was PCR amplified from FLAG-HA-STAMBPL1 (Addgene plasmid #22559), human TBC1 domain containing kinase (TBCK ...
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bioRxiv - Genetics 2020Quote: The tdTomato coding sequence was PCR-amplified from pDEST-HemmarR2 (Addgene # 112813) and cloned into EcoRI/XbaI sites of pUA (Addgene 58372).
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bioRxiv - Genetics 2020Quote: ... Cas9 coding sequence was PCR-amplified using pEDST-APIC-Cas9 (Addgene # 121657) as the template ...
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bioRxiv - Cell Biology 2019Quote: The fluorescent Gal3 reporter was PCR amplified from pEGFP-hGal3 (Addgene #73080), a gift from Tamotsu Yoshimori ...
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bioRxiv - Bioengineering 2021Quote: ... fragments were PCR-amplified and cloned into the px552 plasmid (Addgene #60958) to make px552-U6-CAG-mCherry ...
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bioRxiv - Immunology 2021Quote: ... BRD9-dBD and BRD9-N216A were PCR-amplified from existing vectors (Addgene plasmids #6537968 and #75114-648 ...
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bioRxiv - Cell Biology 2021Quote: ... The resulting PCR product was inserted into pLVpuro-CMV-N-EGFP (Addgene plasmid # 122848 ...
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bioRxiv - Cell Biology 2021Quote: ... PCR fragments of CgB (DNASU) and CLIP (gift from Eric Campeau; RRID:Addgene_29650) were subcloned into pENTR-RIP via Gibson assembly ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR-amplified UGI DNA from pLenti-TRE3G-FNLS-PGK-Puro (Addgene# 110847) and an NheI/PmeI-digested piggyBac dox inducible expression vector PB-TRE-Cas91 including a puromycin selection marker ...
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bioRxiv - Synthetic Biology 2022Quote: ... pCasTn plasmid cloning: ShCAST genes were PCR amplified from pHelper_ShCAST_sgRNA (Addgene #127921). The vector backbones were amplified from plasmids carrying an RK2 origin of replication with a Kanamycin resistance gene for pKSh2 (Addgene #167515 ...
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bioRxiv - Neuroscience 2020Quote: ... dLight1.1 sequence was PCR amplified from pCMV.dLight1.1 (Addgene #111053, Dr. Lin Tian)56 using Q5 polymerase (primers ...
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bioRxiv - Biochemistry 2022Quote: ... mCherry was PCR amplified from mCherry-Alpha-5-Integrin-12 (Addgene #54970) using forward primer 54970RMmCherryaddNHis_F2 ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR with pKD3 (pKD3 was a gift from Barry L. Wanner; Addgene plasmid #45604 ...
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bioRxiv - Genetics 2020Quote: ... The His2Av polyA sequence was PCR amplified from pDEST-APLO (Addgene 112805). DNA fragments were assembled together using NEBuilder DNA Assembly (New England Biolabs Inc.).
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bioRxiv - Neuroscience 2020Quote: ... secA5 sequences were amplified by PCR from pBH-UAS-secA5-YFP (Addgene) and mCherry sequences were amplified by PCR from pAAV-hSyn-mCherry (Addgene ...
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bioRxiv - Neuroscience 2020Quote: ... and mCherry sequences were amplified by PCR from pAAV-hSyn-mCherry (Addgene) sequences ...
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bioRxiv - Biochemistry 2020Quote: ... The EMCV IRES sequence was amplified by PCR from EMCV_IRES_pcDNA4TO_H2B_SunTag24x_v1 (Addgene #246719) using the following primers ...
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bioRxiv - Genomics 2021Quote: ... The PCR amplicon was cloned into a lacZ reporter vector (Addgene #139098) using Gibson assembly (New England Biolabs)57 ...
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bioRxiv - Cancer Biology 2020Quote: ... The MetRSL274G coding sequence was PCR amplified using pMarsL274G construct (Addgene 63177) as a template and inserted into BamHI and MluI sites within the pLV-EF1a-IRES-puro vector (Addgene 85132 ...
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bioRxiv - Cancer Biology 2021Quote: ... TUBA1B was amplified using PCR (donor plasmid: Addgene #57159, from Michael Davidson) and an EGFP-TUBA1B fusion protein was generated with two-stage overhang extension PCR using the TUBA1B and EGFP cDNA fragments ...
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bioRxiv - Molecular Biology 2020Quote: ... The 6xHis-MBP tag was PCR amplified from pDZ2087 construct (Addgene, #92414) with primers MBP F and MBP R ...
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bioRxiv - Cell Biology 2022Quote: ... 24xSuntag (24xGCN4) was PCR-amplified from pcDNA4TO-24xGCN4_v4-kif18b-24xPP7 (Addgene, #74928) and inserted between the BsrGI and ApaI sites of pcDNA-MCP-mCherry.
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bioRxiv - Molecular Biology 2022Quote: ... IκBα DNA was PCR amplified from pBABE-GFP-IκBα-wt (Addgene, 15263) and was digested and cloned into pET28-CBP-His6 ...
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bioRxiv - Neuroscience 2022Quote: ... we first PCR amplified myr- 4xSNAPf from pUAS-myr-4xSNAPf (Addgene #87637) using FRT-containing primers ...
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bioRxiv - Genomics 2022Quote: ... KRAB(ZIM3) was PCR-amplified from pLX303-ZIM3-KRAB-dCas9 (Addgene # 154472). The final construct is KRAB(ZIM3)-dCas9 or Zim3-dCas9 for short ...
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bioRxiv - Neuroscience 2022Quote: ... the YTHDF2 ORF was PCR amplified from pcDNA-flag-YTHDF2 (Addgene, #52300). The resulting amplicon was digested with AgeI and XbaI and cloned into cut sites upstream of HaloTag insert in pGW1-Halo.
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bioRxiv - Neuroscience 2022Quote: ... the YTHDF2 ORF was PCR amplified from pcDNA-flag-YTHDF2 (Addgene, #52300). The resulting amplicon was digested with KpnI and SalI and cloned into cut sites upstream of the 2A in the pGW1-2A-GFP vector.
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bioRxiv - Developmental Biology 2023Quote: ... the PCR amplicon was cloned into a lacZ-reporter vector (Addgene #139098) or mCherry reporter vector (available upon request ...
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bioRxiv - Microbiology 2023Quote: ... mKO2 was PCR amplified from the template mKO2-N1 (Addgene, Cat. #4625) using primers containing matching restriction sites ...
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bioRxiv - Pathology 2023Quote: Cre recombinase DNA was amplified by PCR using pCAG-Cre (Addgene #13775) as a template ...
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bioRxiv - Cancer Biology 2023Quote: ... The IRES site was amplified by PCR from pInducer-21 (Addgene #46948). YAP5SA was amplified by PCR from Addgene #33093 ...
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bioRxiv - Bioengineering 2023Quote: ... CjCas9 was PCR-amplified from pAAV-EFS-CjCas9-eGFP-HIF1a (Addgene #137929) as two overlapping fragments using primers to create D8A and H559A mutations ...