Labshake search
Citations for Addgene :
251 - 300 of 971 citations for hsa let 7f RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... GFP was amplified by PCR from pLeGO-iG2 (Addgene #27341). The IRES site was amplified by PCR from pInducer-21 (Addgene #46948) ...
-
bioRxiv - Cell Biology 2023Quote: ... NEMOD311N was obtained by PCR of pGEX- NEMOD311N (Addgene #11968). GFP was obtained by PCR of a GFP-containing plasmid ...
-
bioRxiv - Cell Biology 2023Quote: ... EBFP2 amplified by PCR from mTagBFP2-C1 plasmid (Addgene #54665), BbsI-silenced Sniper-Cas9 amplified by PCR ...
-
bioRxiv - Systems Biology 2024Quote: ... PCR amplified constructs were inserted into mCherry-CRY2clust (Addgene #105624), using BsrGI/HpaI sites to obtain mCherry tagged JNK2α2 and SgrAI/HpaI sites to obtain mCherry tagged ASK1 constructs ...
-
bioRxiv - Systems Biology 2024Quote: ... was created by PCR cloning of IKK2-EE (Addgene, 11105) with BclI–MfeI ends and inserting into pBABE puro (Addgene ...
-
bioRxiv - Developmental Biology 2019Quote: ... We amplified PCR fragments with the respective primers (see Table S1 for information about the gRNAs and Table S2 for primer sequences used) and 1 ng pCFD6 (Addgene, Cat. No: 73915) as template utilizing Q5 Hot Start High-Fidelity 2X Master Mix (New England Biolabs ...
-
bioRxiv - Neuroscience 2022Quote: ... to enable downstream experiments with GFP based reporters together with this line) was amplified from pJFRC206 (with primers smGFP::v5_hifi_F/R from Addgene #63168, (Nern et al., 2015)) ...
-
bioRxiv - Molecular Biology 2020Quote: ... were PCR amplified from pcDNA4/TO-ORF24-3xFLAG (19) (Addgene #138423) with primers to introduce BamHI and NotI sites and cloned into pcDNA4/TO-3xFLAG (C-terminal tag ...
-
bioRxiv - Molecular Biology 2021Quote: SARS-CoV-2 viral proteins were amplified via PCR from Addgene constructs with a 1x (GGGS ...
-
bioRxiv - Genetics 2020Quote: ... Gal80 coding sequence was PCR amplified from pBPGAL80Uw-4 (Addgene 26235). A His2Av polyA sequence after the BFP/Gal80 coding sequence was PCR amplified from pDEST-HemmarR2 (Addgene # 112813).
-
bioRxiv - Immunology 2021Quote: ... Ovalbumin (OVA) amplicon was PCR amplified from pcDNA3-OVA (Addgene #64599) and cloned into pHIV-Luc-ZsGreen backbone (Addgene #39196) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Positive controls for PCR used lentiguide-Puro EGFP gRNA (Addgene 80036) which contained a gRNA sequence not present in the GeCKO library ...
-
bioRxiv - Cell Biology 2021Quote: ... Smo-GCaMP5G-mCherry was generated by PCR-amplification of GCaMP5G (Addgene plasmid 31788 ...
-
bioRxiv - Cell Biology 2021Quote: ... mKeima was amplified by pcr using mKeima-RED-N1 (Addgene #54597) as a template ...
-
bioRxiv - Synthetic Biology 2021Quote: ... PCR-amplified Cas9n-NG DNA from pX330-SpCas9-NG (Addgene# 117919), PCR-amplified UGI DNA from pLenti-TRE3G-FNLS-PGK-Puro (Addgene# 110847 ...
-
bioRxiv - Cell Biology 2021Quote: ... which was subcloned into the pCR 2.1TOPO vector (AddGene, Cambridge, MA). The antisense digoxigenin-labeled hybridization probes were transcribed in vitro using the T7 MAXIscript kit ...
-
bioRxiv - Microbiology 2021Quote: ... The FLT3 was PCR amplified from pAL119-FLT3L (Addgene, item #21910), and the TBG was amplified from pX602-AAV-TBG:NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA (Addgene ...
-
bioRxiv - Neuroscience 2022Quote: ... and a PCR amplicon containing the CRISPRoff-v2.1 protein from Addgene plasmid 167981 (gifted from Luke Gilbert [45] ...
-
bioRxiv - Genetics 2022Quote: EGFP sequences were amplified by PCR from pGEM5Z(+)-EGFP (Addgene #65206) and were cloned into p(UASp ...
-
bioRxiv - Genetics 2020Quote: ... Gal80 coding sequence was PCR amplified from pBPGAL80Uw-4 (Addgene 26235) using the oligonucleotides aaaaaaaaatcaaaATGAGCGGTACCGATTACAACAAAAGGAGTAGTGTGAG and GCCGACTGGCTTAGTTAattaattctagaTTAAAGCGAGTAGTGGGAGATGTTG ...
-
bioRxiv - Cell Biology 2019Quote: ... pL-EF1a-Puro backbone by PCR on 7TGP vector (Addgene#24305) while hEF1a promoter was amplified from p1494 EF1a Ires Hygro vector [4] ...
-
bioRxiv - Neuroscience 2020Quote: ... Gephyrin sequences were amplified by PCR from pmCherryC2-Gephyrin P1 (Addgene) and subcloned into the pAAV-hSyn-mCherry (Addgene ...
-
bioRxiv - Plant Biology 2019Quote: ... PCR fragments obtained from TurboID-containing plasmid (V5-TbID-NES_pCDNA3, Addgene) and pEarleyGate101 vector were assembled by overlapping ends using Gibson assembly master mix (NEB ...
-
bioRxiv - Cell Biology 2019Quote: ... ErkKTR-BFP was cloned by PCR amplification of TagBFP (Addgene 102350) and insertion into the pHR ErkKTR-iRFP construct used previously (Addgene 111510) ...
-
bioRxiv - Genetics 2020Quote: PE2 coding sequence was PCR amplified from pCMV-PE2 (Addgene # 132775) and gel purified ...
-
bioRxiv - Genetics 2020Quote: PE2 coding sequence was PCR amplified from pCMV-PE2 (Addgene # 132775). pEntr backbone was PCR amplified from pEntr_D-TOPO (Invitrogen K240020) ...
-
bioRxiv - Biochemistry 2021Quote: ... plasmids7 used in droplet digital PCR experiments were sourced from Addgene. The PSP1-targeting spacer was cloned into pHelper by Gibson assembly ...
-
bioRxiv - Cancer Biology 2021Quote: ... mCherry was PCR amplified from pMSCV-Peredox-mCherry-NLS (Addgene, 32385) using primers containing overhangs with the homology sites for GMAP cloning ...
-
bioRxiv - Developmental Biology 2021Quote: ... The PCR products were cloned into the pMAL-c2x (Addgene # 75286) vector ...
-
bioRxiv - Microbiology 2020Quote: ... The PCR products were cloned into pNIC-CH (Addgene plasmid 26117) by ligation-independent cloning (42) ...
-
bioRxiv - Cell Biology 2022Quote: ... and Eg5 were cloned using PCR into plasmid 462 (Addgene 89065) containing a Z3EV promoter (Ohira et al. ...
-
bioRxiv - Cell Biology 2022Quote: ... The ORF of EPLIN isoform alpha was PCR amplified from Addgene plasmid #40928 to generate plasmid #1 ...
-
bioRxiv - Molecular Biology 2022Quote: ... and cloned the PCR products into overexpression vectors EGFP-N1 (Addgene) or pLenti6 (Addgene) ...
-
bioRxiv - Systems Biology 2023Quote: ... and XRCC6 were cloned by PCR into pEN_TT_miRc2 3xFLAG (Addgene #83274)64 ...
-
bioRxiv - Cell Biology 2023Quote: ... KRT5 cDNA was PCR amplified using pBabe mRFP1-KRT5 (Addgene#59493) as template using Phusion High Fidelity Polyemrase (F503 ...
-
bioRxiv - Bioengineering 2023Quote: ... into a PCR-ed fragment of plasmid pBbA2c-RFP (Addgene #35326) to replace the red fluorescent protein (RFP ...
-
bioRxiv - Bioengineering 2023Quote: ... the Cas9 gene was PCR amplified from pwtCas9-bacteria (Addgene #44250) and a gBlock (purchased from Integrated DNA Technologies [IDT] ...
-
bioRxiv - Genetics 2023Quote: ... the CHD7 ORF was PCR amplified from a plasmid (Addgene, 89460) using Phusion High-Fidelity Master Mix (NEB ...
-
bioRxiv - Developmental Biology 2023Quote: ... the iTol2 flanked kanR-cryaa:sfGFP fragment was PCR amplified from Addgene #74153 (Fuentes et al. ...
-
bioRxiv - Developmental Biology 2023Quote: ... PCR amplified 8xtetO-EF1a promoter-eGFP cassette from iDuet101a (Addgene, #17629) was cloned with Gibson assembly in frame with the SV40pA sequences that were PCR amplified from lentiCRIPSRv2 (Addgene ...
-
bioRxiv - Genomics 2022Quote: ... and the previously digested hu6 PCR fragment (from pMJ117 Addgene 85997) with appropriate overhangs was inserted via T4 ligation ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The following expression vectors were amplified by PCR: RAD51 (Addgene #41815), CtIP (Addgene #109403) ...
-
bioRxiv - Cell Biology 2023Quote: ... APEX2 was obtained by PCR from APEX2-csChBP (no. 108876, Addgene) and inserted in pMK-289 (no ...
-
bioRxiv - Neuroscience 2023Quote: ... mScarlet was PCR amplified from the plasmid pmScarlet_C1 (Addgene plasmid #85042) while also adding a fyn myristolyation sequence (a membrane targeting sequence ...
-
bioRxiv - Cell Biology 2023Quote: ... the CRY2 PHR domain was PCR amplified from pCRY2PHR-mCherryN1 (Addgene plasmid #26866 ...
-
bioRxiv - Cell Biology 2023Quote: ... and mEmerald amplified by PCR from mEmerald-Sec61b-C1 (Addgene #90992), with a Gly-Gly-Gly-Ser linker placed either side of the HaloTag or mEmerald at Site 3 and Site 4 ...
-
bioRxiv - Immunology 2024Quote: ... mCherry was amplified by PCR from the mCherry-Rab4a (Addgene #55125) plasmid and inserted with NEBuilder HiFi Assembly cloning kit (#E5520S ...
-
bioRxiv - Systems Biology 2024Quote: ... was created by PCR cloning of 3xHA-MKK6-EE (Addgene, 47579) with BglII–SalI ends and inserting into pBABE puro (Addgene ...
-
bioRxiv - Plant Biology 2023Quote: ... The PCR amplicons were cloned into the pAGM9121 (Addgene plasmid #51833), pICH41308 (Addgene plasmid #47998 ...
-
bioRxiv - Bioengineering 2024Quote: ... Firefly luciferase was PCR amplified from pCDNA-Luciferase (Addgene, cat # 18964) using primers introducing a 5’ KpnI site and a 3’ NheI site ...