Labshake search
Citations for Addgene :
351 - 400 of 946 citations for RatCol 8 mg ml type I collagen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: ... or 20 μg pSuper-cofilin1-shRNA + 20 μg pSuper-ADF-shRNA (Bosch et al., 2014) + 8 μg pCAG-CyRFP1 (Addgene; (Laviv et al., 2016)) + 4 μg EGFP-N1 or 20 μg pSuper-cofilin1-shRNA + 20 μg pSuper-ADF-shRNA + 8 μg pCAG-CyRFP1 + 4 μg shRNA insensitive cofilin1-EGFP (Bosch et al. ...
-
bioRxiv - Cancer Biology 2022Quote: 293T cells were transfected with packaging plasmids (8 μg of pCMV-dR8.2 dVPR and 1 μg pCMV-VSV-G, a gift from Robert Weinberg, Addgene plasmid #8455 and 8454, respectively) along with shRNA plasmids (8 μg ...
-
bioRxiv - Neuroscience 2020Quote: ... The segment containing RFP and shRNA was next sub-cloned to replace CaMP3.0 in AAV-CMV-LOX-STOP-LOX-mG-CaMP3.0 vector (Addgene, #50022) by using Asc I ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were transfected with 1 μg pLentiCMV DEST plasmid + 0.25 mg of each of three 3rd generation lentiviral packaging plasmids (pMDLg/pRRE, pRSV-Rev and pMD2.G, Addgene plasmids #12251 ...
-
Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... and 5 mg sgRNA cloned into the BsmBI restriction site of the MLM3636 plasmid (gift from Keith Joung, Addgene plasmid #43860 ...
-
bioRxiv - Neuroscience 2023Quote: ... a kind gift from Don Arnold) and pENN.AAV.CamKII 0.4.Cre.SV40 (viral titer/ml: 2.3 x 10^13 GC/ml (Addgene 105558-AAV9) in the ratio 2:1:1 ...
-
Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery systembioRxiv - Genetics 2019Quote: ... Specific gRNA for EGFP and RIG-I (Table2, underlined) were cloned according to Golden Gate reaction from ZhangLab protocol (Addgene SAM library sgRNA cloning protocol) using BbsI-HF (NEB ...
-
bioRxiv - Neuroscience 2022Quote: ... Titer: 1.8×1013 GC/mL (Addgene – diluted ½) or 4.5×1012 GC/mL (VVF) ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... 1 × 1013 copies/mL) (Addgene, Watertown, MA) were injected into the PeF (250 nL/side ...
-
bioRxiv - Neuroscience 2022Quote: ... +0.4 ML with diluted (1:3; Addgene) 4 × 0.15 μL of AAV9-Synapsin-jGCaMP7f-WPRE (Addgene) ...
-
bioRxiv - Developmental Biology 2024Quote: ... worms were grown on bacteria expressing double stranded RNA for him-8/klp-16 using the pLT 651 plasmid (Addgene plasmid # 59998, Timmons et al., 2014).
-
bioRxiv - Neuroscience 2021Quote: ... The cells were sparsely labeled by administration of AAVs (CamKII.Cre, 104vg/ml, Addgene # 105558-AAV9 and CAG.Flex.EGFP, 108vg/ml, Addgene #28304-PHPeB) at DIV 9-10 and processed for experiments 10-11 days later.
-
bioRxiv - Neuroscience 2021Quote: ... viral titer of 5.86×1013 particules/ml) and the Cre recombinase (AAV9-hSyn-Cre, from Addgene, 3.3×1013p/ml), diluted at a factor 10 and 100 respectively ...
-
bioRxiv - Neuroscience 2019Quote: ... AAV9 CaMKII-eGFP (3.45×10^12 virus molecules/mL)(Penn) or AAV5-hDlx-ChR2-mCherry (7.6 ×10^14 virus molecules/mL) (Addgene plasmid # 83898 ...
-
bioRxiv - Neuroscience 2019Quote: ... 5.2×1012 gc/ml) and AAV2/5-hSyn-DIO-hM3Dq-mCherry (7.8×1012 gc/ml) were produced from Addgene plasmids #44362 and #44361 at the facility of Nantes University (UMR 1089 ...
-
bioRxiv - Neuroscience 2020Quote: ... The cells were sparsely labeled by administration of AAVs (CamKII.Cre, 104vg/ml, Addgene # 105558-AAV9 and CAG.Flex.EGFP, 108vg/ml, Addgene #28304-PHPeB) at DIV 9-10 and processed for experiments 10-11 days later.
-
bioRxiv - Neuroscience 2023Quote: ... 1.3×1012 vg/mL) was mixed with AAV1-CAG-FLEX-EGFP-WPRE (Addgene; Cat # 51502; ≥ 1×1013 vg/mL) in a 4:1 ratio ...
-
bioRxiv - Neuroscience 2024Quote: ... 1.03 × 1013 vg/mL), and AAV-mCherry (pAAV9-hSyn-mCherry; 114472-AAV9, 9.0 × 1012 vg/mL) viruses were purchased from Addgene. AAV-Rem2-wt (AAV9-hSyn1-Myc-Rem2-WPRE ...
-
bioRxiv - Neuroscience 2021Quote: U118-MG astrocytoma cells were transfected with the F-actin marker mCherry-LifeAct-7 plasmid (gift from Michael Davidson, Addgene #54491) using a calcium phosphate precipitation protocol.24 LifeAct-7 is a 17 amino acid peptide that binds to the actin cytoskeleton ...
-
bioRxiv - Microbiology 2023Quote: ... lentiviral particles pseudotyped with the VSV-G protein were produced by cotransfecting HEK293T cells in 10cm dishes with 5 mg pLentiCMVPuroDEST vector (Addgene, #17452), 2 mg VSV-G Env expression vector pMD2.G (Addgene #12259 ...
-
bioRxiv - Neuroscience 2022Quote: ... opsin construct), >1.3×1013 vector genomes/ml (Neuroscience Center Zurich, control construct) and 1.4 × 1013 GC/mL (Addgene, opsin construct). The viral vectors were aliquoted and stored at -80°C until stereotaxic injection surgeries.
-
bioRxiv - Neuroscience 2022Quote: ... AAV2 Ef1a-DIO-hChR2(H134R)-EYFP (4.2 × 1012 vg/ml, UNC, #AV4378) and AAV9 hSyn-ChrimsonR-tdTomato (2.6 × 1013 vg/ml, Addgene, #59171-AAV9).
-
bioRxiv - Neuroscience 2023Quote: ... titer ≥ 1×1013 vg/mL) and pAAV-CAG-GFP (titer ≥ 7×1012 vg/mL) viral preps were purchased from Addgene (Addegene viral prep # ...
-
bioRxiv - Neuroscience 2024Quote: ... to express channelrhodopsin (ChR2) or control virus (n = 4, AAV-Ef1a-fDIO-mCherry, 200 mL, 3 × 1012 GC/mL; Serotype: 9; Addgene) was unilaterally injected over 10 minutes into the MePD using a 2-µL Hamilton microsyringe (Esslab ...
-
bioRxiv - Cell Biology 2019Quote: ... The K18-Scarlet-I reporter was constructed by cloning a fusion of the K18 construct from pMK1253 in frame with the C-terminal mScarlet-I (Addgene #98839, a gift from Dorus Gadella (65)) as well as replacing the EF1a promoter with a CAG promoter to obtain pJC49.
-
bioRxiv - Cell Biology 2020Quote: ... and 0.5/ml µg pMD2.G (Addgene: 12259) was prepared ...
-
bioRxiv - Neuroscience 2019Quote: ... AAV5-syn-GCaMP6s (titer: 1Χ1013 vg/mL, Addgene), or AAV.PHPeB-syn-GCaMP6s (titer ...
-
bioRxiv - Neuroscience 2019Quote: ... AAV2-retro-CAG-tdTomato (7.0E12 GC/ml, Addgene) or AAV-PHP.S-CAG-tdTomato (1.7E13 ...
-
bioRxiv - Neuroscience 2020Quote: ... AAV8-hSyn-mCherry (2.1×1013 vg/mL, Addgene). AAV2-retro-EF1a-DIO-FLPo (2.6×1013 vg/ml ...
-
bioRxiv - Neuroscience 2022Quote: ... AAV5-CAG-mCherry (1.0 × 10e13 GC/ml, Addgene) and AAV5-EF1a-DIO-eYFP (1.0 × 10e13 GC/ml ...
-
bioRxiv - Neuroscience 2024Quote: ... Undiluted AAV1-ChR2 (Addgene; 1013 vg/mL titre) was injected into right binocular V1 (bV1 ...
-
bioRxiv - Neuroscience 2024Quote: ... Undiluted AAV1-ChrimsonR (Addgene; 1013 vg/mL titre) or diluted (1:10 in saline ...
-
bioRxiv - Neuroscience 2024Quote: - AAV2retro hSyn.Cre.WPRE.hGH (1.8 × 1013 gp/ml) (Addgene #105553)
-
bioRxiv - Neuroscience 2024Quote: ... pAAV5-hSyn-DIO-mCherry (Addgene, 50459, vg/mL), pAAV5-hSyn-DIO-hM4D(Gi)-mCherry (Addgene ...
-
bioRxiv - Microbiology 2021Quote: Chemical-genetic screens were initiated by thawing 5 × 1 mL (1 OD600 unit per mL) aliquots of the Mtb CRISPRi library (RLC12; Addgene #163954) and inoculating each aliquot into 19 mL 7H9 supplemented with kanamycin (10 μg/ml ...
-
bioRxiv - Neuroscience 2023Quote: ... 2.3 x 1013 vg/mL) or 200 nL/side AAV5-CMV-HI-eGFP-Cre-WPRE-SV4 (Addgene; ≥ 1 x 1013 vg/mL) was injected at a rate of 100 nL/min into the insula of Pdynlox/lox and Oprk1lox/lox mice ...
-
bioRxiv - Neuroscience 2023Quote: ... AAV5, 1.7×e13 gene copies/mL) or pAAV-hSyn-EGFP (#50465, AAV5, 1.2×e13 gene copies/mL) (Addgene, Inc., Watertown, MA, USA) were diluted to 8.5×e11 gene copies/mL using PBS (Gibco® Dulbecco’s phosphate-buffered saline ...
-
bioRxiv - Neuroscience 2020Quote: ... titer value ≥ 7×1012 vg/ml (Addgene, #59462-AAVrg).
-
bioRxiv - Neuroscience 2020Quote: ... AAV9.CAGGS.FLEX.ChR2.tdTomato (titer: 2.44e12 GC/ml; Addgene 18917) or AAV1.Syn.FLEX.Chronos.GFP (titer ...
-
bioRxiv - Neuroscience 2020Quote: ... or AAV1.Syn.FLEX.Chronos.GFP (titer: 4.80e12 GC/ml; Addgene 62722)) ...
-
bioRxiv - Developmental Biology 2022Quote: ... pCXLE-L-MYC-F2A-LIN28 (ML, Addgene ID 27080), pCXLE-hOCT4-shTP53 (Addgene ID 27077) ...
-
bioRxiv - Physiology 2023Quote: ... pAAV-EF1α-DIO-mCherry (3.6×1012 vg/ml, Addgene plasmid #50462-AAV5 ...
-
bioRxiv - Neuroscience 2022Quote: AAV2/1 hsyn-jGCaMP8m-WPRE (Addgene, 2.0E13 GC/ml)
-
bioRxiv - Neuroscience 2022Quote: AAV2/1 hSyn-SF-iGluSnFR.A1848 (Addgene, 3E12 GC/ml)
-
bioRxiv - Neuroscience 2022Quote: AAV2/rg hSyn-DIO-mCherry (Addgene, 9E12 GC/ml)
-
bioRxiv - Neuroscience 2022Quote: AAV2/1 hsyn-jGCaMP8s-WPRE (Addgene, 2.8E13 GC/ml)
-
bioRxiv - Neuroscience 2023Quote: ... pAAV5-hSyn-mCherry (≥ 7 x 1012vg/mL, Addgene # 114472) or AAVrg-hSyn-Cre (≥ 1.8 x 1013 vg/ml ...
-
bioRxiv - Neuroscience 2023Quote: ... typical dose 6.0×108 gc/mL) and a reporter virus (AAV-PHP-eB_7x-TRE-tdTomato, typical dose 1.8×1011 gc/mL) (Addgene plasmid id: #191210 and, #191207). The viral titers of the tTA virus used were empirically adjusted based on the Cre driver line to yield sparsely labeled brains ...
-
bioRxiv - Neuroscience 2024Quote: ... All rats received bilateral microinjections of AAV2.CMV::nNOS-shRNA-EGFP or AAV2.CMV::luciferase-shRNA-EGFP (USC viral vector core: titer∼∼7×1013 vg/mL) combined with an AAV2.CAG::Flex-Ruby2sm-Flag.WPRE.SV40 (Addgene: titer: ∼1×1012 vg/ml) into the NAc ...
-
bioRxiv - Neuroscience 2022Quote: ... or ssAAV-9/2-hEF1a-dlox-eNpHR3.0_iRFP(rev)-dlox-WPRE-hGHp(A) (ETHZ/UZH Viral Vector Facility, titre: 7.2 × 1012 vg/mL) or pAAV-flex-GFP (Addgene, titer: 2.5 ×1013 vg/mL) for activation ...