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Citations for Addgene :
101 - 150 of 1157 citations for Rat Phosphatidic Acid Phosphatase Type 2A PPAP2A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Plasmid pCX-HO1-2A-EGFP was ordered from Addgene. Goat-anti-Mouse IgG+IgM (10 nm gold ...
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bioRxiv - Biochemistry 2023Quote: pCAG-EGFP or pCX-HO1-2A-EGFP (Addgene, #74672) were transfected using a standard Lipofectamine 2000 protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... or Lenti-Cas9-2A-Blast (SU-DIPG-XXXVI; Addgene) was supplemented into the cell media and incubated for 72 h ...
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bioRxiv - Neuroscience 2023Quote: ... either AAV5-hSyn-FLEx-GFP-2A-Synaptophysin-mRuby (Addgene plasmid #71760 gifted by Liqun Luo48 ...
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Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signallingbioRxiv - Developmental Biology 2023Quote: ... or pSpCas9(BB)-2A-GFP (PX458) (Addgene, Plasmid #48138) according to standard protocol 57.
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bioRxiv - Cell Biology 2023Quote: ... and cloned into pSpCas9(BB)-2A-GFP vector (RRID:Addgene_48138). The successful insertion of the sgRNAs was verified by Sanger sequencing ...
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bioRxiv - Neuroscience 2023Quote: ... or pLVX-UbC-rtTA-Ngn2:2A: EGFP (Addgene #84777) [23 ...
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bioRxiv - Neuroscience 2022Quote: ... mice were first injected with a helper virus (AAV5- hSyn-FLEX-splitTVA-2A-mCherry-2A-B19G; produced in-house, modified after Addgene plasmid no. 52473) into MEC ...
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bioRxiv - Neuroscience 2022Quote: The pCMV-hEAAT2 plasmid encoding the human excitatory amino acid transporter type 2 (EAAT2) was a gift from Susan Amara (Addgene plasmid # 32814 ...
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bioRxiv - Molecular Biology 2022Quote: px458SpCas9GFP (SpCas9-2A-GFP) and px459SpCas9PuroR (SpCas9-2A-puro) vectors were previously obtained from Feng Zhang through Addgene (Addgene plasmid # 48138 and plasmid # 62988) for transfection into mammalian cell lines[64] ...
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bioRxiv - Cancer Biology 2023Quote: ... PDTO were transduced with lentiviral supernatants for pKLV2-U6-gRNA5(Empty)-PGK-BFP-2A-GFP-W and pKLV2-U6-gRNA5(gGFP)-PGK-BFP-2A-GFP-W (Addgene plasmids # 67979 and# 67980) [32] ...
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bioRxiv - Neuroscience 2021Quote: ... a pSpCas9(BB)-2A-GFP plasmid (Addgene, catalog no. 48138) and paired gRNAs targeting exon 5 of SCN2A were nucleofected into the 50B iPSCs using the Amaxa 4D-nucleofector with code CA137 ...
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bioRxiv - Genomics 2019Quote: ... These guides were ordered from Sigma Aldrich and cloned into pSpCas9(BB)-2A-GFP (PX458, Addgene #48138). A stably transfected clonal MCF7 cell line expressing mStrawberry and luciferase (pCLIIP-EF1-LS ...
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bioRxiv - Cancer Biology 2020Quote: The PX459V2.0 plasmid (pSpCas9(BB)-2A-Puro) (Addgene, Watertown, MA) was used to ligate the appropriate single guide RNA sequences 5’ of the trans-activating CRISPR RNA (tracrRNA ...
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bioRxiv - Cell Biology 2019Quote: ... pSpCas9(BB)-2A-GFP vector was used (PX458, Addgene, #48138). For CRISPR activation (CRISPRa) ...
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bioRxiv - Biochemistry 2021Quote: ... cloned into plasmid pSpCas9(BB)-2A-Puro (Addgene. ID 48139). Plasmids were transfected into B16-F1 cells ...
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bioRxiv - Cell Biology 2021Quote: ... short EF1a promoter (EFS) expresses SaCas9-2A-PuromycinR (Addgene, 96921).
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bioRxiv - Systems Biology 2020Quote: ... annealed and cloned into pSpCas9(BB)-2A-Puro (Addgene #48139) vector plasmids ...
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bioRxiv - Neuroscience 2020Quote: ... and pAAV-EF1a-cre off EGFP-2A-TVA (Addgene 126470) was used ...
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bioRxiv - Cell Biology 2021Quote: ... using AAVS1 SA-2A-puro-pA donor plasmid (#22075, Addgene) as a template ...
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bioRxiv - Cancer Biology 2020Quote: The pSpCas9(BB)-2A-Puro(PX459) v2.0 plasmid (Addgene #62988) was used to clone guide sequences targeting Got2 per supplier’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: The pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene plasmid: 62988) was used ...
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bioRxiv - Immunology 2022Quote: Cells were transfected with pSpCas9(BB)-2A-GFP (px458, Addgene) containing guide RNAs targeting genes cGas ...
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bioRxiv - Molecular Biology 2019Quote: ... digested and dephosphorylated pSpCas9(BB)-2A-puro vector (Addgene, 62988). The resulting constructs were transfected into HeLa cells using LipofectamineTM 2000 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... using pSpCas9(BB)-2A-Puro(PX459) V2.0 vector (62988; Addgene). The following DNA sense and antisense strands of the targeting sites were annealed with each other in KOD FX neo buffer (Toyobo) ...
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bioRxiv - Genetics 2020Quote: The pSpCas9(BB)-2A-Puro (PX459) vector (Addgene plasmid #62988) (36 ...
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bioRxiv - Molecular Biology 2021Quote: ... or pSpCas9(BB)-2A-RFP (modified from Addgene plasmid #48138) as described previously67 ...
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bioRxiv - Molecular Biology 2021Quote: pSpCas9n(BB)-2A-Puro (PX462) vector was purchased from Addgene. For the establishment of PNKP-/- cells using CRISPR/Cas9 system ...
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bioRxiv - Genetics 2019Quote: ... pSpCas9(BB)-2A-Puro(PX459)V2.0 from Feng Zhang (Addgene plasmid # 62988 ...
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bioRxiv - Genomics 2021Quote: pSPCas9-2A-puromycin plasmid was purchased from AddGene (ref 62988). The plasmid that we generated ...
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bioRxiv - Cell Biology 2020Quote: ... cloned into pSpCas9(BB)-2A-GFP (PX458) (Addgene plasmid # 48138)60 ...
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bioRxiv - Developmental Biology 2021Quote: ... The vector OCT4-2A-eGFP-PKG-Puro (plasmid #31938, Addgene) was used as template to generate the donor vector ...
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bioRxiv - Cell Biology 2022Quote: ... cloned into the pSpCas9(BB)-2A-Puro plasmid (Addgene #62988) and transfected into MDA-MB-231 cells using Lipofectamine® 3000 (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... Switzerland and cloned into pSpCas9(BB)-2A-Puro (Addgene 48139) following the cloning strategy described by Ran et al ...
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bioRxiv - Cancer Biology 2022Quote: ... using pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene Plasmid #62988) and PX458-GFP (Addgene Plasmid #48138) ...
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Chromatin priming elements direct tissue-specific gene activity prior to hematopoietic specificationbioRxiv - Genomics 2023Quote: ... (pSpCas9(BB)-2A-GFP (PX458) was a gift from Feng Zhang (Addgene plasmid # 48138 ...
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bioRxiv - Molecular Biology 2023Quote: ... we electroporated hAPCs with pSpCas9(BB)-2A-GFP (#48138, Addgene) containing the AAVS1-T2 targeting guide and pSH-EFIRES-P-AtAFB2 (#129715 ...
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bioRxiv - Cell Biology 2023Quote: ... pSpCas9(BB)-2A-GFP (PX458) was from Feng Zhang (Addgene plasmid # 48138 ...
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bioRxiv - Neuroscience 2023Quote: ... The Cas9-guideRNA plasmid pSpCas9(BB)-2A-GFP (Addgene, 48138) was obtained from Addgene ...
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bioRxiv - Cancer Biology 2023Quote: ... The pSpCas9(BB)-2A-Puro (PX459) V2.0 plasmid (Addgene #62988) containing a tdimer2-specific gRNA (guide ...
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bioRxiv - Bioengineering 2023Quote: The vector pSpCas9(BB)-2A-GFP (PX458) (Addgene plasmid #48138) containing the human-codon-optimized SpCas9 gene with 2A-GFP and the sgRNA backbone ...
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bioRxiv - Developmental Biology 2024Quote: ... The vector pSpCas9(BB)-2A-GFP (PX458) (Addgene, Plasmid 48138), was purchased to target NEUROG2 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were transduced with Lenti-Cas9-2A-Blast (Addgene #73310) lentiviral particles followed by selection with 10μg/ml blasticidin (Invivogen #ant-bl ...
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bioRxiv - Molecular Biology 2024Quote: Plasmid pSpCas9(BB)-2A-GFP (PX458) was obtained from Addgene #48138 (Watertown ...
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bioRxiv - Cell Biology 2024Quote: ... The pSpCas9(BB)-2A-Puro (PX459) V2.0 plasmid (Addgene, #62988) was linearised using by BbsI-HF (New England Biolabs ...
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bioRxiv - Genetics 2024Quote: ... pLenti-EF1a-SPdCas9-DNMT3A-2A-Blast (dCas9-DNMT3A; Addgene#71216), pLenti-EF1a-SPdCas9-DNMT3A(E752A)-2A-Blast (dCas9-inactiveDNMT3A ...
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bioRxiv - Genetics 2024Quote: ... Plenti-EF1a-SPdCas9-DNMT3B-2A-Blast (dCas9-DNMT3B) (Addgene#71217), pCCL-PGK-SPdCas9-BFP-DNMT1(dCas9-DNMT1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Munich) or pHAGE-RSV-tdTomato-2A-GCaMP6s vector (#80316 Addgene). Both plasmids allow simultaneous Ca2+ activity recordings and morphometric analyses of the cancer cells36 ...
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bioRxiv - Physiology 2021Quote: ... GFP-2A-SynmRuby (Addgene plasmid #71760, a gift from Liqun Luo), HA-Cas9 (Addgene plasmid #61592 ...
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bioRxiv - Physiology 2021Quote: ... Caspase3-2A-TEVp (Addgene plasmid #45580, a gift from Nirao Shah), GCaMP6s (Addgene plasmid #100845 ...