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Citations for Addgene :
501 - 550 of 2187 citations for Rat Epithelial Discoidin Domain Containing Receptor 1 DDR1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Mediobasal hypothalamic FKBP51 acts as a molecular switch linking autophagy to whole-body metabolismbioRxiv - Neuroscience 2021Quote: ... A viral vector containing a Cre expressing cassette (pAAV-CMV-HI-eGFP-Cre-WPRE-SV40, Addgene; #105545) was used to induce Fkbp5 deletion in Fkbp5lox/lox mice ...
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bioRxiv - Cancer Biology 2020Quote: The vector containing RON (MST1R) pDONR223-MST1R was a gift from William Hahn and David Root (Addgene plasmid # 23942 ...
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bioRxiv - Biophysics 2020Quote: ... Two plasmids containing full-length untagged Npl4 and C-terminal His-tagged Ufd1 were purchased from Addgene. Npl4 fragments were expressed in pET-28a vector with an N-terminal His-SUMO tag ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting DNA fragment containing two MpU6promoter-gRNA cassettes was transferred into pMpGE010 (cat. no. 71536, Addgene) (Sugano et al. ...
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bioRxiv - Genetics 2021Quote: ... Constructs containing different sgRNAs (500 ng) were co-transfected with pCMV-ABE7.10 (2000 ng, Addgene plasmid # 102919) into cells by using LipofectamineTM 3000 (Thermo Fishers ...
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bioRxiv - Neuroscience 2021Quote: ... we co-transfected neurofilament medium chain (NFM) cDNA-containing plasmid pmNFM (a gift from Anthony Brown, Addgene plasmid #83126 ...
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bioRxiv - Cancer Biology 2020Quote: ... or vector containing the dominant negative TP53 construct (pBABE-hygro p53 DD, Bob Weinberg, Addgene plasmid #9058) into the clonal MCF10-2A TetOn-CENPA-FLAG-HA cell line by lentiviral transduction ...
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bioRxiv - Genomics 2021Quote: ... Early passage primary MEFs were transformed with SV-40 T antigen containing plasmid pBSSVD2005 (ADDGENE, Cambridge, MA) to generate immortalized MEFs ...
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bioRxiv - Biochemistry 2022Quote: AR-LBD (663-919) containing an N-terminal His-tag and encoded in pET15b plasmid (Addgene #89083) was expressed in Rosetta (DE3 ...
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bioRxiv - Biochemistry 2022Quote: A pET28a plasmid containing full-length human β-catenin was gifted from Randall Moon (Addgene plasmid # 17198) and various fragments of the coding region (residues 1-137 (NTERM) ...
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bioRxiv - Plant Biology 2023Quote: ... NJ) or amplified by PCR with overhangs containing SapI recognition sites and assembled into pUAP4 (Addgene #136079) to create parts compatible with the Phytobrick standard (74) ...
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bioRxiv - Cell Biology 2023Quote: CRISPRi sgRNAs were cloned into either BFP- or GFP-containing lentiviral vectors (Addgene plasmids #60955 and #111596). To generate double knockdown cells sgRNAs were ligated into IGI-P1589 containing a Neomycin resistance cassette ...
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bioRxiv - Biochemistry 2023Quote: A pProEx-IDE-wt (#99014) plasmid containing cloned human IDE (Met42–Leu1019) was purchased from Addgene (UK). The plasmid encoded a 6-histidine tag at the N-terminus of the protein ...
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bioRxiv - Immunology 2023Quote: ... BRAFV600E PTEN-/- melanoma cells were transduced with lentivirus containing the pSCAR-Cas9-blast-GFP vector (Addgene: #162074). This system ...
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bioRxiv - Cell Biology 2023Quote: ... The PCR cassette was transfected into HeLa cells with a helper plasmid containing AsCas12a endonuclease (Addgene 89353). Following cleavage ...
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bioRxiv - Neuroscience 2023Quote: Plasmid containing iGluSnFR(A184S) under the control of the human synapsin promoter was purchased from Addgene (#106174) and packaged in the AAV8-Y733F serotype 55 ...
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bioRxiv - Biochemistry 2023Quote: ... DNA oligos containing guide RNA (gRNA) sequences were cloned into pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene, #42230). The oligos were phosphorylated with T4 PNK (NEB ...
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bioRxiv - Molecular Biology 2023Quote: Dual luciferase constructs containing intervening viral RNAs of interest were made using the pSGDLucV3.0 vector (Addgene 119760). Insertion sequences were ordered as double-stranded DNA gene fragments (IDT ...
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bioRxiv - Molecular Biology 2023Quote: ... fragments containing promoter and fusion protein segments were digested and cloned into the pKHR4 vector (Addgene #74592) using SpeI (NEB #R3133 ...
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bioRxiv - Developmental Biology 2023Quote: PCR amplification of the repair template was performed using 50 ng of plasmid containing mNeonGreen (Addgene 125134) as template in a touchdown PCR reaction (annealing temperature decreased 1°C from 65°C to 50°C for the first 15 cycles followed by 20 cycles with an annealing temperature of 50°C ...
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bioRxiv - Cell Biology 2023Quote: ... Cloning of Def16 sequence to pET plasmid containing an N-terminus Hisx6 tag and GFP (Addgene # 29663) was done by whole plasmid amplification using the indicated forward and reverse primers for GFP-Def16 (primers 1 and 2 ...
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bioRxiv - Cell Biology 2024Quote: ... For C-terminal tagging a homology directed repair donor plasmid containing mClover3 (derived from Addgene plasmid 72829), followed by 24xMS2V5 33 was created ...
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bioRxiv - Cell Biology 2024Quote: ... They were used in conjunction with a Cas9 expression vector containing neomycin (G418) resistance transcript (Addgene #98292). H293T cells were transfected with lentivirus packaging plasmids and plasmids carrying either sgRNAs or Cas9 ...
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bioRxiv - Genomics 2022Quote: ... elegans Vector Kit was a gift from Andrew Fire (Addgene kit # 1000000001). A translational reporter was later generated by synthesizing the remainder of the coding sequence (IDT ...
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bioRxiv - Cell Biology 2021Quote: ... 0.25 μL of F-ABM lentiviral mix (1:1:1:1 of Addgene plasmids 27150 ...
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bioRxiv - Synthetic Biology 2023Quote: ... We used DNA parts provided with the EMMA cloning kit (Addgene kit # 1000000119) and generated DNA fragments by PCR using Platinum™ SuperFi II Green PCR Master Mix (ThermoFisher ...
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bioRxiv - Cancer Biology 2021Quote: ... the LentiCRISPR v2 plasmid containing Cas9 and including a puromycin selection marker was purchased from Addgene (Plasmid #52961). A 20 nucleotide sgRNA sequence targeting exon 3 of mouse lgals3 was designed using the website http://crispr.mit.edu/ ...
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bioRxiv - Cell Biology 2020Quote: ... a fragment containing the 3×Flag-nls-cas9-nls coding sequence isolated from pBS-Hsp70-cas9 (Addgene, #46294) with same enzymes was inserted ...
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bioRxiv - Molecular Biology 2020Quote: ... AsCas12a and SpCas9 nucleases were expressed from the pET-based T7 promoter-containing plasmids (Addgene, Plasmids #90095, #62374) in Escherichia coli strain BL21-DE3 as described (3 ...
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bioRxiv - Molecular Biology 2019Quote: A DNA fragment containing HTT103Q-GFP was amplified from p426 103Q GPD (Addgene #1184; (Krobitsch and Lindquist, 2000)) and inserted into pCAGEN by NEBuilder HiFi DNA Assembly Master Mix (NEB).
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bioRxiv - Cell Biology 2022Quote: - MOVAS GFP/progerin: retroviral particles containing pBABE-puro-GFP-progerin which was a gift from Tom Misteli (Addgene plasmid # 17663 and PBABE-puro which was a gift from Hartmut Land & Jay Morgenstern & Bob Weinberg (Addgene plasmid # 1764 ...
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bioRxiv - Neuroscience 2019Quote: For control conditions we used a plasmid containing EGFP under the CAG promoter (pCAG-EGFP, Addgene plasmid #11150) (Matsuda and Cepko ...
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bioRxiv - Molecular Biology 2019Quote: ... The single vector mammalian expression system containing a CAG promoter-driven St1Cas9 LMD-9 and its U6-driven sgRNA (U6_sgRNA_CAG_hSt1Cas9_LMD9; Addgene plasmid #110626) was built from the above-described plasmids ...
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bioRxiv - Synthetic Biology 2021Quote: ... Finally the mAID- mCherry cassette containing a selection marker was amplified from pMK292 mAID-mCherry2- NeoR (Addgene #7283033) or pMK293 mAID-mCherry2-Hygro (Addgene #7283133 ...
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bioRxiv - Cell Biology 2021Quote: ... A single stranded oligo (IDT) was synthesized containing the sense sequence and then cloned into PX458 (Addgene #48138) and transfected into RPE1 cells ...
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bioRxiv - Biochemistry 2020Quote: ... cDNA encoding wild-type human ubiquitin containing an N-terminal HA-tag was expressed from pRK5-HA (Addgene). Full-length EGFP fused N-terminally to a nuclear localization signal (NLS ...
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bioRxiv - Microbiology 2020Quote: ... DNA oligos (IDT) containing sgRNA sequences were annealed and ligated into pX458 (Addgene, #48138, gift from Feng Zhang). Cells were transfected with pX458 constructs using Mirus TransIT-X2 (Mirus Bio ...
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bioRxiv - Genomics 2021Quote: ... gRNA sequences were designed with chopchop (https://chopchop.cbu.uib.no) and corresponding DNA oligonucleotides containing BsmBI overhangs were annealed and ligated with lentiCRISPR v2 plasmid (Addgene, # 52961), which contains both Cas9 and sgRNA sequences55 ...
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bioRxiv - Cancer Biology 2021Quote: ... pBabe-puro plasmids containing human C/EBPB LAP2 and LIP isoforms were from Addgene (Cat.# 15712 and 15713).
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bioRxiv - Developmental Biology 2022Quote: ... 2010) are annotated in Addgene as containing DENDRA2 (e.g., pEG545, Addgene plasmid #40116 and pEG345, Addgene plasmid #40077). DENDRA2 is a modified form of DENDRA that has an important single amino acid change near the C-terminus (A224V ...
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bioRxiv - Neuroscience 2021Quote: ... 450nl of a mostly anterograde virus containing the Cre recombinase under CamKII promoter to target pyramidal cells (AAV1_CamKII_Cre_SV40, Addgene, USA) was injected into the right MEC (+3.2mm laterally from Lambda along the lambdoid suture and DV -2.5mm from skull level) ...
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bioRxiv - Neuroscience 2020Quote: Primers containing linkers attached to each target without the PAM sequence were used for PCR with pCFD4 (RRID:Addgene_49411) as a template ...
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bioRxiv - Microbiology 2020Quote: ... and a transfer vector containing GFP (lentiCas9-EGFP, a gift from Phillip Sharp and Feng Zhang, Addgene #63592)43 in which we deleted Cas9 ...
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bioRxiv - Synthetic Biology 2019Quote: ... The fragment containing the LbCpf1 coding sequence was amplified from pY016 hLbCpf1 (Addgene plasmid # 69988; http://n2t.net/addgene:69988; RRID:Addgene_69988) {Zetsche ...
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bioRxiv - Synthetic Biology 2021Quote: ... CA) or amplified by PCR with overhangs containing BpiI (BbsI) recognition sites and assembled into pUAP1 (Addgene #63674) to create parts compatible with the phytobrick standard (3) ...
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bioRxiv - Cell Biology 2019Quote: ... phosphorylated oligonucleotides containing each target sequence into BbsI-digested pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene plasmid # 62988) 46 ...
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bioRxiv - Neuroscience 2021Quote: ... The visual cortex was injected with a total of 2–3 μl of virus solution (1e13 GC/ml) containing AAV9-Syn-GCaMP6s.WPRE.SV40 (Penn Vector Core or Addgene) through a beveled glass micropipette (tip size 10–20 μm diameter ...
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bioRxiv - Neuroscience 2021Quote: ... we co-transfected neurofilament medium chain (NFM) cDNA-containing plasmid pmNFM (a gift from Anthony Brown, Addgene plasmid #83126; http://n2t.net/addgene:83126;RRID:Addgene_83126)63.
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bioRxiv - Genomics 2020Quote: ... we amplified Ultramers with primers containing sequence homologous to either the STARR-seq luciferase validation vector_mP_empty (Addgene# 99298) or pGL4.23 (Promega ...
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bioRxiv - Cell Biology 2021Quote: MEF cell lines were transduced with lentivirus containing Cas9 and guide RNA in the lentiCRISPRv2 plasmid (Addgene, 52961) and selected using 2 μg/ml puromycin ...