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Citations for Addgene :
51 - 100 of 1322 citations for PDGF BB Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and pSpCas9(BB)-2A-GFP(PX458) (Addgene #48138) with BbsI (NEB ...
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bioRxiv - Cell Biology 2023Quote: The pSpCas9(BB)-2A-GFP (Addgene, Cat. 48138) plasmid was linearized using BbsI (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... an oligo duplex encoding the sgRNA sequence was cloned at the Bbs I site of pX458-pSpCas9 (BB)-2A-GFP plasmid (Addgene #48138). To construct a donor vector ...
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bioRxiv - Cell Biology 2021Quote: ... pSpCas9(BB)-2A-GFP (pX458) and pSpCas9(BB)-2A-puro (pX459) were a gift from Feng Zhang (Addgene #48138 and #48139) (Ran et al. ...
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bioRxiv - Cell Biology 2023Quote: ... This phosphorylated DNA fragment was ligated to the Bbs I cloning site in pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene plasmid # 62988) [16] ...
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bioRxiv - Physiology 2021Quote: ... were annealed and cloned into the BbsI digested pSpCas9(BB)-2A-GFP vector (SpCas9(BB)-2A-GFP (PX458) which was a gift from Feng Zhang (Addgene plasmid # 48138). U2OS cells were transfected in 6-well dishes with 2ug of pSpCas9(BB)-2A-GFP plasmid containing the respective sgRNA using Lipofectamine 2000 (Life Technologies ...
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bioRxiv - Genetics 2019Quote: ... Both gRNAs were cloned into the pSpCas9(BB)-2A-GFP (PX458) and pSpCas9(BB)-2A-Puro (PX459) V2.0 from Feng Zhang (Addgene #48138, #62988 respectively) according to the protocol described in (31) ...
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bioRxiv - Cell Biology 2021Quote: ... sgDNA oligonucleotides were annealed and cloned into the BbsI digested pSpCas9(BB)-2A-GFP vector (SpCas9(BB)-2A-GFP (PX458) was a gift from Feng Zhang (Addgene plasmid # 48138). MEFs were transfected with 5ug of pSpCas9(BB)-2A-GFP plasmid containing the respective sgRNA using Lipofectamine 3000 (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: We used pSpCas9 (BB)-2A-Puro (PX459) V2.0 (Addgene) and modified it in order to replace the CMV promoter by the EF1 alpha promoter ...
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bioRxiv - Cell Biology 2021Quote: ... pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene plasmid #62988) from Feng Zhang ...
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bioRxiv - Immunology 2021Quote: ... and cloned into pSpCas9(BB)-2A-GFP (PX458, Addgene), according to the published protocol (Ran et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... or pSpCas9(BB)-2A-Puro (PX459) vector (Addgene, 48139) from Feng Zhang ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmid pSpCas9(BB)-2A-Puro (PX459) V2.0 (#62988, Addgene) with puromycin resistance was used as the target plasmid to carry the guide RNA ...
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bioRxiv - Cancer Biology 2020Quote: ... pSpCas9(BB)-2A-GFP (PX458) was purchased from Addgene.
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bioRxiv - Molecular Biology 2020Quote: ... 2.5 μg of pSpCas9(BB)-2A-GFP (PX458, Addgene) plasmid was transfected into cells using Lipofectamine 3000 (ThermoFischer Scientific) ...
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bioRxiv - Molecular Biology 2019Quote: ... using the plasmid pSpCas9(BB)-2A-GFP (Addgene, #113194) as a starting point instead of pAC2-dual-dCas9VP48-sgExpression ...
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bioRxiv - Microbiology 2020Quote: ... and pSpCas9(BB)-2A-Puro (PX459, Addgene ID 48139). pX458 and pX459 were digested with BbsI and dephosphorylated with FastAP Thermosensitive Alkaline Phosphatase ...
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bioRxiv - Genetics 2020Quote: ... and pSpCas9n(BB)-2A-Puro (PX461) (plasmid#48141, Addgene) respectively ...
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bioRxiv - Cancer Biology 2021Quote: ... pSpCAS9 (BB)-2A-Puro (PX459) V2 plasmids (Addgene; #62988) were used following the protocol described by Ran et al.54 ...
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bioRxiv - Cell Biology 2020Quote: ... pSpCas9(BB)-2A-Puro v2.0 (Plasmid #62988 from Addgene). sgRNAs were designed using CHOPCHOP v2 (46) ...
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bioRxiv - Cell Biology 2021Quote: ... The pSpCas9(BB)-2A-GFP vector (Addgene plasmid # 48138) was cut with BbsI (NEB ...
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bioRxiv - Microbiology 2019Quote: ... This was cloned into pSpCas9(BB)-2A-GFP (Addgene) as described (29) ...
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bioRxiv - Genomics 2022Quote: ... or pSpCas9(BB)-2A-GFP (pCas9-GFP, Addgene #48138) plasmids using BbsI Golden Gate reactions as described45 ...
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bioRxiv - Cancer Biology 2023Quote: ... pSpCas9(BB)-2A-Puro (PX459) V2.0 (pX459_puro, Addgene #62988), pSpCas9(BB)-2A-Hygro (pX459_hygro ...
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Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signallingbioRxiv - Developmental Biology 2023Quote: ... or pSpCas9(BB)-2A-GFP (PX458) (Addgene, Plasmid #48138) according to standard protocol 57.
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bioRxiv - Cell Biology 2023Quote: ... and cloned into pSpCas9(BB)-2A-GFP vector (RRID:Addgene_48138). The successful insertion of the sgRNAs was verified by Sanger sequencing ...
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bioRxiv - Systems Biology 2023Quote: ... and cloned into pSpCas9(BB)P2A-Puro (Addgene #48139) vector plasmids ...
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bioRxiv - Neuroscience 2021Quote: ... a pSpCas9(BB)-2A-GFP plasmid (Addgene, catalog no. 48138) and paired gRNAs targeting exon 5 of SCN2A were nucleofected into the 50B iPSCs using the Amaxa 4D-nucleofector with code CA137 ...
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bioRxiv - Genomics 2019Quote: ... These guides were ordered from Sigma Aldrich and cloned into pSpCas9(BB)-2A-GFP (PX458, Addgene #48138). A stably transfected clonal MCF7 cell line expressing mStrawberry and luciferase (pCLIIP-EF1-LS ...
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bioRxiv - Cancer Biology 2020Quote: The PX459V2.0 plasmid (pSpCas9(BB)-2A-Puro) (Addgene, Watertown, MA) was used to ligate the appropriate single guide RNA sequences 5’ of the trans-activating CRISPR RNA (tracrRNA ...
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bioRxiv - Cell Biology 2019Quote: ... pSpCas9(BB)-2A-GFP vector was used (PX458, Addgene, #48138). For CRISPR activation (CRISPRa) ...
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bioRxiv - Biochemistry 2021Quote: ... cloned into plasmid pSpCas9(BB)-2A-Puro (Addgene. ID 48139). Plasmids were transfected into B16-F1 cells ...
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bioRxiv - Systems Biology 2020Quote: ... annealed and cloned into pSpCas9(BB)-2A-Puro (Addgene #48139) vector plasmids ...
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bioRxiv - Cancer Biology 2020Quote: The pSpCas9(BB)-2A-Puro(PX459) v2.0 plasmid (Addgene #62988) was used to clone guide sequences targeting Got2 per supplier’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: The pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene plasmid: 62988) was used ...
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bioRxiv - Neuroscience 2021Quote: ... pyogenes with 2A-EGFP pSpCas9 (BB)-2A-GFP (PX458) (Addgene plasmid # 48138 ...
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bioRxiv - Immunology 2022Quote: Cells were transfected with pSpCas9(BB)-2A-GFP (px458, Addgene) containing guide RNAs targeting genes cGas ...
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bioRxiv - Molecular Biology 2019Quote: ... digested and dephosphorylated pSpCas9(BB)-2A-puro vector (Addgene, 62988). The resulting constructs were transfected into HeLa cells using LipofectamineTM 2000 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... CRISPR Cas9 plasmids were obtained from Addgene (#62988, pSpCas9(BB)-2A-Puro PX459 V2.0)
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bioRxiv - Cell Biology 2020Quote: ... using pSpCas9(BB)-2A-Puro(PX459) V2.0 vector (62988; Addgene). The following DNA sense and antisense strands of the targeting sites were annealed with each other in KOD FX neo buffer (Toyobo) ...
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bioRxiv - Genetics 2020Quote: The pSpCas9(BB)-2A-Puro (PX459) vector (Addgene plasmid #62988) (36 ...
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bioRxiv - Molecular Biology 2021Quote: ... or pSpCas9(BB)-2A-RFP (modified from Addgene plasmid #48138) as described previously67 ...
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bioRxiv - Molecular Biology 2021Quote: pSpCas9n(BB)-2A-Puro (PX462) vector was purchased from Addgene. For the establishment of PNKP-/- cells using CRISPR/Cas9 system ...
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bioRxiv - Genetics 2019Quote: ... pSpCas9(BB)-2A-Puro(PX459)V2.0 from Feng Zhang (Addgene plasmid # 62988 ...
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bioRxiv - Cell Biology 2020Quote: ... cloned into pSpCas9(BB)-2A-GFP (PX458) (Addgene plasmid # 48138)60 ...
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bioRxiv - Cell Biology 2022Quote: ... cloned into the pSpCas9(BB)-2A-Puro plasmid (Addgene #62988) and transfected into MDA-MB-231 cells using Lipofectamine® 3000 (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... Switzerland and cloned into pSpCas9(BB)-2A-Puro (Addgene 48139) following the cloning strategy described by Ran et al ...
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bioRxiv - Cancer Biology 2022Quote: ... using pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene Plasmid #62988) and PX458-GFP (Addgene Plasmid #48138) ...
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bioRxiv - Molecular Biology 2023Quote: ... we electroporated hAPCs with pSpCas9(BB)-2A-GFP (#48138, Addgene) containing the AAVS1-T2 targeting guide and pSH-EFIRES-P-AtAFB2 (#129715 ...
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bioRxiv - Cell Biology 2023Quote: ... pSpCas9(BB)-2A-GFP (PX458) was from Feng Zhang (Addgene plasmid # 48138 ...