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Citations for Addgene :
601 - 650 of 819 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and the lentivector backbone was Gibson-assembled with a miniU6-tracrRNA gene block and U1a-SpyCas9 amplified by PCR from Addgene plasmid #121507 ...
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bioRxiv - Cell Biology 2023Quote: ... In vitro target specific gRNA cleavage activity was validated by transfecting N2A cells with PCR amplified gRNA gblock and Cas9 plasmid DNA (px330, Addgene) using ROCHE Xtremegene HP ...
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bioRxiv - Cell Biology 2022Quote: ... MLKL gene and its truncated version MLKL(1-182) were amplified by standard PCR from pRetrX-TRE3G-hMLKL-Venus (Addgene_106078) using primers MLKL_Fw and MLKL_Rv for the first ...
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bioRxiv - Molecular Biology 2023Quote: ... The Nexn coding sequence was PCR amplified from the cDNA of murine hearts and subcloned into pFLAG-MRTFA (Addgene #11978) to create the pFLAG-Nexn plasmid.
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bioRxiv - Molecular Biology 2023Quote: ... Sequences encoding Ifi205 ORF and Ifi205K364ER299E ORF were amplified by PCR then subcloned between the BamHI and EcoRI restriction sites of pGEX-6P-1(Addgene). Ifi205 proteins were expressed as GST-Ifi205-FLAG-fusions in the E ...
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bioRxiv - Bioengineering 2023Quote: ... the coding sequence was amplified by PCR from pVITRO1-Trastuzumab-IgG1/κ which was a gift from Andrew Beavil (Addgene plasmid # 61883 ...
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bioRxiv - Cell Biology 2023Quote: ... Olivier Pertz (University of Bern, Switzerland)44 was PCR-amplified and inserted into pLV-EF1a-MCS-IRES-Blast (Addgene 85133) using Gibson assembly88 ...
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bioRxiv - Bioengineering 2023Quote: ... the 13.5-kb SphI-digested and gel-extracted eDA83 was ligated with a 980-bp PCR-amplified KanR (using oligos 321/322 and vector pUC57-Kan (Addgene) as a template) ...
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bioRxiv - Biochemistry 2023Quote: The coding sequence for MZT2A was PCR amplified from pACEBac1-ZZ-TEV-MZT2-3C-mEGFP (a gift from Tarun Kapoor (Addgene plasmid # 178074 ...
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bioRxiv - Biochemistry 2023Quote: The coding sequence for amino acids 1-110 of GCP2 were PCR amplified from pACEBac1-gamma-TuSC (a gift from Tarun Kapoor (Addgene plasmid # 178079 ...
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bioRxiv - Cancer Biology 2023Quote: Human AKT1_D274A was generated by overlapping PCR mutagenesis using wild-type AKT1 (a gift from Thomas Leonard, Addgene plasmid 8656133) and cloned into a pAceBac1 vector with N-terminal His10-StrepII-(tev ...
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bioRxiv - Cell Biology 2023Quote: ... the ORF was amplified by PCR using Halo-SMARCA5 (#FHC01216; Kazusa) as a template and inserted into sfGFP-N1 vector (#54737; Addgene) digested with EcoRI using the In-Fusion HD Cloning Kit (Takara Bio) ...
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bioRxiv - Cancer Biology 2023Quote: pVHL-stabilized HIF-2α (P405A/P531A) was amplified by PCR from the HA-HIF2alpha-P405A/P531A-pcDNA3 plasmid (Addgene, 18956) using primers BamHI-HIF2Af and AscI-HIF2Ar using NEB Q5 High-Fidelity 2X Master Mix according to the instructions of the manufacturer ...
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bioRxiv - Genetics 2023Quote: The denAsCas12a open reading frame was PCR amplified from pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776) (Addgene plasmid # 107943 ...
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bioRxiv - Microbiology 2024Quote: ... 782 bp PCR product obtained with primers ymCherryU and ymCherryL and pAG426-GAL-ccdb-ymCherry (a gift from Susan Lindquist (Addgene plasmid # 14155 ...
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bioRxiv - Cell Biology 2024Quote: The coding sequence of APEX2 was PCR-amplified from APEX2 plasmid (a gift from Alice Ting, Addgene plasmid #72480, [86]) using specific primers containing restriction sites NotI (5’ ...
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bioRxiv - Molecular Biology 2024Quote: ... the FRT-STOP-FRT cassette sequence was PCR amplified from pJFRC201-10xUAS-FRT-STOP-FRT-myr::smGFP-HA (#63166; Addgene). The coding sequence of mCD8::DQVD/A was PCR-amplified from the genomic DNA of Caspase-Sensitive/Insensitive-Gal4 [20] ...
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bioRxiv - Molecular Biology 2024Quote: ... the coding sequence of mNeonGreen was PCR-amplified from pAc5-V5::mNeonGreen [17] and ligated into the EcoRI-digested pQUAST (#24349, Addgene) vector using In-Fusion ...
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bioRxiv - Genetics 2024Quote: ... pLenti-CMV-HA-HHIP lentiviral expression plasmid was generated by subcloning the appropriate human HHIP cDNA PCR fragment into pLenti-CMV Blast (659–1) (Addgene). HA-tag was inserted into human HHIP cDNA after Ala 23 ...
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bioRxiv - Cell Biology 2020Quote: ... were annealed in annealing buffer (10mM Tris pH 8.0, 50mM NaCl, 1mM EDTA) and cloned in pENTR/pSUPER+ entry vector (Addgene#17338) which was recombined into pLenti X1 Puro DEST (Addgene#17297) ...
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bioRxiv - Plant Biology 2019Quote: ... K353E, and D450N), RBOHD/C (full-length, C1, C2, and C3) were amplified by PCR and cloned into pOPINK (Addgene, #41143) or pOPINM (Addgene ...
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bioRxiv - Molecular Biology 2020Quote: ... A C-terminal Strep tag on ORF24-NTD was added by inverse PCR to generate p6H-SUMO3-ORF24-NTD-Strep (Addgene #138467).
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bioRxiv - Cell Biology 2020Quote: For derivation of HA-Smo and Smo-HA ESCs mouse Smo was amplified by PCR from the pGEN-mSmo (Addgene, #37673) and introduced in the PB-HA-IRES-Neo vector ...
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bioRxiv - Cell Biology 2020Quote: ... To generate PCP-NLS-2xmCherry, we PCR amplified PCP from phage UbiC NLS HA stdPCP st-dGFP (Horvathova et al., 2017) (Addgene #104099) using primer sequences 5’-ATACTCGAGCGCCAC-CATGGGCCCAAA and 5’-CTCATCGATGGTGGC-GACCGGTGGAC ...
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bioRxiv - Cell Biology 2020Quote: ... To generate MCP-NLS-2xGFP, we PCR amplified 2xEGFP from phage UbiC NLS HA stdPCP stdGFP (Horvathova et al., 2017) (Addgene #104099) using primer sequences 5’-TCTATCGATATGGTGAGCAAGGG-CGAG and 5’-ATAGCGGCCGCTTATTTGTACAAT-TCATCCATACCATGGG ...
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bioRxiv - Cell Biology 2020Quote: ... To generate the modified TREAT reporter constructs, we first PCR amplified humanized Renilla luciferase from the original TREAT plasmid (Horvathova et al., 2017) (Addgene #104096) using primer sequences 5’-ATACTCGAGCGACTCACTATAGGCTAGCCAC and 5’-ATAGGCCGGCCTTACTGCTCGTTCTTCAG-CAC ...
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bioRxiv - Cell Biology 2020Quote: ... We then excised the IRES-H2B-RFP cassette and cloned in place a 5’ copy of YFP lacking a stop codon, which we PCR amplified from IRES-H2B-YFP-DD (Han et al., 2014) (Addgene #96893) using primer sequenc-es 5’-AAGTTATCGATATGGTGAGCAAGGGCGAGG and 5’-GCATGGACGAGCTGTACAAGAGCGGC-CGCATGGT ...
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bioRxiv - Genetics 2021Quote: ... For the pPB_TRE3G::dCas9-5XGCN4_EF1a::TetOn-Hygro, the Streptococcus pyogenes dCas9GCN4 was PCR amplified from the PlatTET-gRNA2 plasmid (Morita et al., 2016) (Addgene #82559), and cloned together with a d2 destabilization domain under control of the TRE3G promoter in a PiggyBac backbone vector also containing the TET-ON3G transactivator and the Hygromycin resistance gene separated by an IRES sequence and under control of the CAG promoter ...
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bioRxiv - Molecular Biology 2021Quote: ... To express TagRFP658 and mCardinal in neurons under CaMKII promoter the corresponding genes were PCR amplified and swapped with the Arch-GFP gene in FCK-Arch-GFP (Addgene #22217) using InFusion cloning ...
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bioRxiv - Neuroscience 2021Quote: ... was PCR amplified from the pBid-UAS-myr plasmid (Akbergenova et al., 2018) and fused with the GCaMP7s cDNA (Addgene # 104463) (Dana et al. ...
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bioRxiv - Neuroscience 2021Quote: ... The donor was made by amplifying homology arms from the genome by PCR and fusing them by Gibson assembly with a cDNA coding for 6xHis-mClover3 (Addgene #74252) (Bajar et al. ...
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bioRxiv - Molecular Biology 2021Quote: We in-house generated a lentiviral construct expressing dCas9-KRAB-MeCP2 by PCR amplification of the dCas9-KRAB-MeCP2 (contains a domain of MeCP2) from pB-CAGGS-dCas9-KRAB-MeCP2 (Addgene 110824), and then insert it to the pLenti-EF1a-dCas9-VP64-2A-Blast backbone (Addgene 61425 ...
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bioRxiv - Cell Biology 2022Quote: CymR was constructed via PCR and Gibson Assembly from the following constructs: pCuo CA Rac1 CMV + cumate operon purchased from Addgene (#84643), human APC open reading frame purchased from Addgene (#16507) ...
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bioRxiv - Cell Biology 2022Quote: ... The mCherry2-NSP2 expressing plasmids were created by cloning a PCR-amplified NSP2 fragment into the mCherry2-C1 (Addgene plasmid 54563) and mCherry2-N1 vectors (Addgene plasmid 54517 ...
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bioRxiv - Molecular Biology 2021Quote: ... GFP ORF was PCR-amplified and cloned between AgeI and EcoRI sited of lentiviral vector with synapsin I promoter (Addgene #20945). During this cloning AgeI site was destroyed and a new AgeI was introduced on a PCR primer downstream of GFP ...
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bioRxiv - Genomics 2020Quote: ... The INTS6 ORF was PCR amplified with 5’ Xho I and 3’ EcoRI restriction site overhangs and the coding sequence for a C-terminal V5 epitope tag was added in frame to the 3’ end of the INTS6 ORF (Key Resources Table. The INTS6 PCR product and MSCVpuro vector (Addgene 68469) were digested with XhoI (NEB R0146 ...
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bioRxiv - Molecular Biology 2019Quote: ... The linear donor was generated by PCR and gel purified from a plasmid harboring a synthetic AID-P2A-Hygromycin insert (pMGS54, Addgene #126583). We amplified the insertion using primers that contain 50 nucleotide homology tails ...
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bioRxiv - Genomics 2019Quote: ... the NLSSV40-dCas9-3X NLSSV40-24X GCN4-V4-NLSSV40-P2A-BFP fragment was amplified by PCR from plasmid pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE (Addgene Plasmid #60910) and then ligated into PiggyBac plasmid pB-TRE3G-BsmBI-EF1α-PuroR-P2A-rtTA by Golden Gate Assembly with enzyme BsmBI and T4 ligase (NEB) ...
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bioRxiv - Systems Biology 2019Quote: ... we mixed competent yeast with promoter and GFP or mCherry PCR products and 60ng of linearized expression vector BSP188 (Addgene #110917). This expression vector contains the unc-54 terminator and chromosome II MosSCI homology arms for integration at ttTi5605 ...
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bioRxiv - Molecular Biology 2019Quote: The pLenti-EF1-osTir1-9Myc-P2A-Bsd was constructed by PCR amplifying the Oryza Sativa Tir1 (osTir1) cDNA from the pBabe Puro osTIR1-9Myc (Addgene #80074) and inserted into the AgeI/BamHI site of the lentiCas9-Blast construct (Addgene #52962).
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bioRxiv - Cell Biology 2019Quote: ... BbsI digested fragment containing gRNA core and dU6:3 promoter was PCR amplified from pCFD4-U6:1_U6:3-tandemgRNAs (gift from Simon Bullock (Addgene plasmid # 49411) (Port et al. ...
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bioRxiv - Bioengineering 2021Quote: ... The nCas9(D10A) was generated by the PCR method using previously optimized Cas9 as a template (Level 1 hCas9 module, Addgene #49771). Desired sgRNA sequence was PCR amplified using plasmid pICH86966::AtU6p::sgRNA_PDS (Addgene #46966 ...
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bioRxiv - Bioengineering 2021Quote: ... nanobody sequences were PCR amplified with primers containing terminal regions of homology for cloning into a pRset plasmid (Addgene plasmid 3991)(Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... The UAS sites and K10 3’UTR were replaced via PCR with the squash promoter and squash 3’UTR from pBS-Squ-mCherry (Eric Wieschaus56, Addgene 20163). The RhoGEF2 ORF was obtained from the DGRC (SD04476) ...
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bioRxiv - Cell Biology 2021Quote: These constructs were subcloned via PCR from the corresponding squ-mNeongreen-RhoGEF2 full length constructs into Ac5-Stable2-neo (Rose Barrio and James Sutherland60, Addgene 32426).
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bioRxiv - Cell Biology 2021Quote: These constructs were subcloned via PCR from the corresponding squ-mNeongreen-RhoGEF2 full length constructs into pGEX6P1-N-HA (Andrew Jackson and Martin Reijns, Addgene 119756).
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bioRxiv - Neuroscience 2020Quote: ... pAAV-hsyn1-mGFP was generated by inserting PCR-amplified mGFP between restriction sites BamHI and HindIII of pAAV-hSyn1-ChR2-EGFP (Addgene #58881). The plasmid pAAV-Ef1a-DIO-hChR2(E123T/T159C)-EYFP was obtained from Addgene (#35509).
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bioRxiv - Neuroscience 2020Quote: To generate pAAV-hsyn1-mGFP-T2A-Cre we amplified EGFP-T2A-Cre by PCR from pRetroX-GFP-T2A-Cre (Addgene #63704) and inserted this fragment between restriction sites BamHI and HindIII of pAAV-hSyn1-ChR2-EGFP (Addgene #58881) ...
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bioRxiv - Biochemistry 2020Quote: ... BsaI sites were added to ends by PCR with complementary overhangs for golden gate cloning into pATT-Dest (Addgene plasmid #79770) using primers MBP-BsaI-GG-F and MBP-BsaI-GG-R (the sequences for all primers used for cloning can be found in Table S1) ...
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bioRxiv - Cell Biology 2021Quote: ... The expression vector for N-terminally FLAG-tagged mouse SYDE2 was generated by PCR amplification of the coding sequence from pNICE HA-mSYD1B (Addgene #59362) and insertion into pcDNA3-FLAG by Gibson assembly.