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Citations for Addgene :
451 - 500 of 819 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... CA) or amplified by PCR with overhangs containing BpiI (BbsI) recognition sites and assembled into pUAP1 (Addgene #63674) to create parts compatible with the phytobrick standard (3) ...
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Using optogenetics to link myosin patterns to contractile cell behaviors during convergent extensionbioRxiv - Developmental Biology 2021Quote: ... the full RhoGEF2 cDNA of Drosophila melanogaster was amplified by PCR from plasmid UAS-akkordion dNhe (Addgene 41976) (71) ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were transfected with 1 ug total DNA (0.5 ug PCR product and 0.5 ug AsCpf1_TATV Cas12 [Addgene: 89354]) using Xtreme-GENE 9 following manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... the cDNA of human CAV1 was PCR amplified from the mammalian expression plasmid Emerald-CAV1 C-10 (Addgene No ...
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bioRxiv - Molecular Biology 2021Quote: ... and miRFP2 genes were PCR amplified and swapped with mClover2 in pmClover2-tubulin-C-18 plasmid (Addgene #56376) and with TagRFP675 in pTagRFP675-actin-C1 (Addgene #44277 ...
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bioRxiv - Immunology 2020Quote: Soluble human ACE2 with an Fc tag was constructed by PCR amplifying ACE2 (residues 1-615) from Addgene plasmid #1786 (a kind gift from Jesse Bloom ...
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bioRxiv - Cell Biology 2022Quote: ... SA-KDEL-IRES and SBP PCR fragments were separately amplified from Str-KDEL_SBP-EGFP-GPI (gift from Franck Perez, Addgene plasmid #65294; RRID:Addgene_65294)28 ...
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bioRxiv - Cell Biology 2022Quote: ... sfGFP was PCR amplified from sfGFP-C1 (gift from Michael Davidson and Geoffrey Waldo, Addgene plasmid #54579; RRID:Addgene_54579)65 ...
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bioRxiv - Cell Biology 2022Quote: ... sfGFP was PCR amplified from sfGFP-C1 (gift from Michael Davidson and Geoffrey Waldo, Addgene plasmid #54579; RRID:Addgene_54579)65 ...
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bioRxiv - Genetics 2022Quote: ... The PCR product was cloned via Gibson assembly into AgeI and EcoRI digested pLKO.1 vector (Addgene#8453). Ligated libraries were electroporated into DH5a electrocompetent cells (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... the Adamts2 or TGFβR2 coding sequence was amplified by PCR using mouse Adamts2 and TGFβR2 ORF plasmids (pCMV-Adamts2, Harvard plasmid clones; pCMV-TGFβR2, Addgene) as templates ...
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bioRxiv - Synthetic Biology 2022Quote: ... we transfected 1μg of PCR-amplified dsDNA reporter into naïve mESCs together with spCas9 plasmid pX459 (Addgene #62988), carrying a single gRNA complementary to the genomic integration site ...
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bioRxiv - Microbiology 2022Quote: ... PCR fragments were cloned into the expression vector pOPINE (gift from Ray Owens; plasmid #26043, Addgene, Watertown, USA) to generate constructs ...
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bioRxiv - Cell Biology 2022Quote: Plasmids encoding GFP-2A-ORF6 were generated by inserting EGFP-T2A sequence (copied from Addgene #140424 by PCR) at the 5’ end of the ORF6 gene in pSecTag2 mammalian expression vector using Gibson Assembly ...
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bioRxiv - Molecular Biology 2023Quote: The DNA fragment encoding dLwaCas13a was PCR-amplified using pC035-dLwaCas13a-msfGFP (a gift from Feng Zhang; Addgene plasmid #91925 ...
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bioRxiv - Plant Biology 2023Quote: ... Each gRNA PCR fragment was individually assembled with Arabidopsis U6 small RNA promoter (pICSL01009::AtU6p, AddGene no. 46968) in the appropriate Level 1 vector (plCH47751 ...
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bioRxiv - Cell Biology 2023Quote: ... were created via PCR amplification (see primer list) and assembled into a Spe-1 digested pCFJ151 (Addgene #19330) vector using isothermal assembly (Gibson et al. ...
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bioRxiv - Cell Biology 2023Quote: ... with I532A mutation and RRRG degron) was amplified by PCR from pBMN-HA-YFP-LOV24 (Addgene, Plasmid #49570) (78 ...
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bioRxiv - Developmental Biology 2023Quote: The T7 promoter sequence was added to the Cas9 coding region by PCR amplification of px260 (Addgene, 42229) using the primer pair listed in Table S3 ...
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bioRxiv - Cell Biology 2023Quote: ... The coding sequence of full-length synaptopodin was generated by PCR and subcloned into the pKH3 vector (Addgene). IP assays were performed basically according to the method described previously (59 ...
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bioRxiv - Cell Biology 2023Quote: A PIP-FUCCI coded DNA fragment was PCR amplified with template DNA (pENTR-PIP-FUCCI; Addgene; plasmid #118621), which was a gift from Jean Cook ...
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bioRxiv - Molecular Biology 2023Quote: ... The TIS11B coding sequence was amplified from pcDNA3.1-puro-GFP-TIS11B using TIS11B MCP F and TIS11B MCP R primers and the TIAL1 coding sequence was PCR amplified from pFRT_TO_FlagHA_TIAL1 (Addgene 106090) using TIAL1 MCP F and TIAL1 MCP R primers.
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: ... PCR was used to amplify the T2A-EGFP sequence from pXR001 (Addgene #109049; a gift from Patrick Hsu44) using the primers T2A-GFP Gibson Forward and Reverse (Supplementary Table 1) ...
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Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... The resultant PCR products were subcloned into BamHI- and SspI-digested pET-His6-Sumo-TEV-LIC (Addgene # 29659) using in-fusion cloning kit (Takara ...
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bioRxiv - Synthetic Biology 2023Quote: ... The DNA fragments of TPPP FL and FKBP were produced by PCR using existing templates and (Addgene #31184). The tagBFP gene was synthesized as gBlocks (Integrated DNA Technologies) ...
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bioRxiv - Cell Biology 2023Quote: ... Hsp90-GFP was first constructed by PCR amplifying HA-Hsp90 (a gift from William Sessa, Addgene plasmid #22487) and recombining into GFP pBabe puro digested with EcoRI-XhoI ...
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bioRxiv - Molecular Biology 2024Quote: The full length BRD4 DNA fragment was amplified by PCR from pcDNA4-TO-HA-BRD4FL (Addgene plasmid #31351)61 incorporated into linearized FM5 lentiviral vectors containing standardized linkers (generously provided by David Sanders ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... This promoter sequence was PCR amplified with Mfe1 and Not1 and inserted into the pUB_smHA_KDM5B_MS2 (Addgene plasmid # 81085) lentiviral vector29 replacing the UbC promoter ...
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bioRxiv - Cancer Biology 2024Quote: ... mRFP with a C-terminal KDEL sequence (ER-mRFP) was PCR amplified out of ER-mRFP (Addgene, 62236) using the forward primer 5’-GGTCGGATCCGCCGCCACCATGGACAGCAAAGG-3’ and the reverse primer 5’-GAGGCACCGGTGTTTAGAGCTCATCTTT-3’ ...
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bioRxiv - Cell Biology 2024Quote: The AAVS1 destination vector was assembled by PCR amplification and Gibson assembly from the pDTA-TK from Robert Benezra’s group (RRID:Addgene_22677) (Nam and Benezra 2009 ...
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bioRxiv - Synthetic Biology 2020Quote: The library was synthesized by Agilent and then resuspended in 100 uL of elution buffer before cloning into plasmid pLibacceptorV2 (Addgene ID no. 106250). The transcription factor spacing library was ordered separate from the other libraries ...
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bioRxiv - Cell Biology 2022Quote: ... These oligos were annealed in annealing buffer (10 mM Tris pH 7.5-8.0, 50 mM NaCl, 1 mM EDTA) as recommended by Addgene (https://www.addgene.org/protocols/annealed-oligo-cloning) ...
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bioRxiv - Synthetic Biology 2021Quote: ... The resulting PCR fragments were cloned into pJUMP27 (pJUMP27-1AsfGFP was a gift from Chris French, Addgene plasmid #126974) (45 ...
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bioRxiv - Molecular Biology 2021Quote: ... a region bearing six let-7 binding sites was PCR-amplified from MSCV puro let-7 sponge (Addgene #29766) and cloned between AgeI and EcoRI sites downstream of GFP.
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bioRxiv - Cancer Biology 2020Quote: ... The PCR product was digested with EcoRI and ligated into EcoRI digested pHR-SFFV-KRAB-dCas9 vector (Addgene, 60954), to generate a pHR-pTRE3G-KRAB-dCas9 vector ...
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bioRxiv - Neuroscience 2019Quote: ... The T2A-GFP sequence was amplified by PCR using plasmid PX461 (a gift from Feng Zhang; Addgene plasmid #48140) as template and inserted into the intermediate plasmid at HindIII site to generate pAAV-hSyn-FLPo-T2A-GFP ...
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bioRxiv - Synthetic Biology 2021Quote: ... multiple fragments were PCR amplified from different donor plasmids and assembled as follow: pMK232 CMV-OsTIR1-PURO (Addgene #7283433) was used as donor plasmid for the expression of OsTIR1 from the AAVS1 locus ...
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bioRxiv - Cell Biology 2020Quote: The gRNAscaffHygroR vector was constructed by PCR amplification of the Hygromycin resistance gene from pBABE-hygro-hTERT (Addgene # 1773) plasmid and cloning into the EGFP_SV40PA vector downstream of the OmEF1a promoter followed by the modified guide RNA scaffold sequence PCR amplified from gRNA_GFP-T2 (Addgene # 41820).
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bioRxiv - Neuroscience 2019Quote: GFP-progerin cDNA was PCR amplified from the pBABE-puro-GFP-progerin plasmid (Addgene, 17663 deposited by Tom Misteli) using primers (forward 5’-GATCATCGATATGGTGAGCAAGGGCGAGGAG-3’ ...
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bioRxiv - Biochemistry 2020Quote: BsaI sites and compatible overhangs were added by PCR amplification of cpGFP from pTKEI-Mal-B2 (Addgene Plasmid #79756) using primers cpGFP-BsaI-GG-F and cpGFP-BsaI-GG-R ...
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bioRxiv - Cell Biology 2021Quote: ... the open reading frames of mTagBFP2 and mNeongreen2 were amplified by PCR from donor vectors pBAD-mTagBFP2 (Addgene #34632) and pSFFV_mNG2(11)1-10 (Addgene #82610) ...
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bioRxiv - Microbiology 2021Quote: ... the human ACE2 coding sequence (RefSeq accession NM_001371415.1) was PCR amplified and cloned into the BamHi site of lentiviral vector pHR-PGK (Addgene #79120). Lentivirus was produced as described above and used to transduce 5×104 target cells (A549 ...
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bioRxiv - Systems Biology 2020Quote: ... and S415A) were introduced using the delitto perfetto method (Stuckey et al., 2011) using the PCR-amplified pCORE cassette (RRID:Addgene_72231) to integrate selective markers at the endogenous gene loci ...
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bioRxiv - Cell Biology 2021Quote: ... mScarlet-i-H2A construct was amplified by PCR from pmScarlet-i_H2A_C1 (a gift from Dorus Gadella, Addgene plasmid #85053) (Bindels et al. ...
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bioRxiv - Cell Biology 2021Quote: ... the EB3-tdTomato fragment was amplified by PCR from EB3-tdTomato (a gift from Erik Dent, Addgene plasmid #50708) (Merriam et al. ...
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bioRxiv - Neuroscience 2021Quote: ... TVA-mCherry was amplified by PCR from a plasmid (gift from Dr. Uchida, Addgene plasmid #38044 ; http://n2t.net/addgene:38044 ; RRID:Addgene_38044, ref43) and inserted into a 5xUAS vector34.
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bioRxiv - Developmental Biology 2022Quote: ... 100µl of the ESCs were then mixed with the scCRISPR construct (Composition: 10µl elute of HDR PCR product; 1µl sgPal7 (Addgene, 71484); 1µl spCas9-BlastR (Addgene ...
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bioRxiv - Cell Biology 2020Quote: ... NTR candidates were PCR amplified and cloned into the NdeI and SalI sites of two plasmids: pUCX (Addgene #60681), for biological overexpression assays ...
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bioRxiv - Neuroscience 2022Quote: ... the DNA sequence encoding hPMCA2w/b was PCR amplified from pZac2.1-GfaABC1D-mCherry-hPMCA2w/b (a gift from Baljit Khakh (Addgene plasmid # 111568 ...
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bioRxiv - Cell Biology 2019Quote: ... The enhanced green fluorescent protein (EGFP) sequence was amplified via PCR from a pcDNA3-EGFP plasmid that was a gift from Doug Golenbock (RRID:Addgene_13031). The EGFP sequence was cloned in-frame on the N-terminus of TMEM135 in the pTarget vector using BamHI and XhoI restriction enzymes ...