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Citations for Addgene :
51 - 100 of 256 citations for Medium Kit without Serum and with CultureBoost R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: TALENs (AAVS1-TALEN-L and AAVS1-TALEN-R; Addgene, 59025/59026)(Gonzalez et al. ...
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bioRxiv - Cell Biology 2020Quote: ... CMV-mito-R-GECO1 was a gift from Robert Campbell (Addgene plasmid #460218 ...
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bioRxiv - Microbiology 2023Quote: ... originally obtained from the laboratory of Sydney Kustu.94 Perturbations of intracellular ppGpp concentrations were performed using a genetic system as described in Büke et al.70 These plasmids (without fluorescent tags) were ordered from AddGene (pRelA’ AddGeneID:175595 ...
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bioRxiv - Microbiology 2022Quote: ... Then pLNCX myr-Akt_K179M (Addgene #9906, a gift of William R. Sellers) was digested with SmaI + DraIII to release a 289 bp fragment containing the K179M mutation in the context of AKT1 ...
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bioRxiv - Neuroscience 2021Quote: ... For the mScarlet the human H2B clustered histone 11 (H2BC11) ( accession #NM_021058) was fused in frame without a linker and cloned into the pAAV-CAG-tdTomato (Addgene #59462) using the sites KpnI and EcoRI at the 5 and 3 prime end respectively ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293FT cells were co-transfected with the lentiviral constructs (pLX-304 vector with/without ATF3 ORF) and 2nd generation viral packaging plasmids VSV.G (Addgene #14888) and psPAX2 (Addgene #12260) ...
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bioRxiv - Neuroscience 2022Quote: ... and fused in frame without a linker to human H2B (H2BC11) (accession #NM_021058) and cloned into the pAAV-CAG-tdTomato (Addgene #59462) using the sites KpnI and EcoRI at the 5 and 3 prime end respectively ...
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bioRxiv - Developmental Biology 2023Quote: ... guides designed to target the chr21P copy without cutting the chr21M/M2 alleles were cloned into PX459V2 (Addgene plasmid #48139) 85 at the BbsI site by annealing and ligating overlapping oligos ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA encoding RNF43 without stop codon was amplified by the PCR and cloned into the pcDNA3.1 MCS-BirA(R118G)-HA (Addgene plasmid #36047) using HpaI (ER1031 ...
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bioRxiv - Immunology 2020Quote: ... Human G3BP1 cDNAs with or without stop codon were amplified by PCR from pN1/G3BP1-iRFP (Okada lab plasmids, Addgene #129339) with the sense primer 5′ -GCCAGATCTATGGTGATGGAGAAGCCTAG-3′ and the antisense primers 5′ -GCCGAATTCGGATCCTTACTGCCGTGGCGC-3′ or 5′ -GCCGAATTCGGATCCCTGCCGTGGCGCAAG-3′ ...
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bioRxiv - Genetics 2022Quote: ... a full-length RHO cDNA with or without the T17M mutation was cloned into a lentiviral expression vector FUGW (Addgene, # 14883), and the RHO CDS was inserted under the control of the UbC promoter ...
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bioRxiv - Genetics 2023Quote: ... Based on these results we replaced the intronized Cas9 with one that had no introns but retained the NLS sequences at the termini (Cas9 without introns from pTX168, which was a gift from Daniel Voytas, Addgene #89257)78 ...
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bioRxiv - Molecular Biology 2022Quote: ... This construct was commercially synthesized (GeneArt) and cloned into 13S-R (Addgene # 48328) to produce pCR_Array/I-G (Table S4) ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 ng of CB1-GFP and 100 ng of R-GECO (Addgene #32444) were mixed with 0.2 µL of lipofectamine 2000 transfection reagent ...
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bioRxiv - Genetics 2023Quote: ... 2μg of both TALEN-L and TALEN-R plasmids (Addgene, #59025 and #59026) and 4µg of pUCM-AAVS1-TO-hNGN2 plasmid (Addgene ...
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bioRxiv - Cancer Biology 2020Quote: ... NCI-H3122 and EKVX cells were stably transfected with pKrasG12C or its homologous GFP backbone plasmid without KrasG12C (pC; Addgene #64336; Bicistronic_GFP_ires_puro) using previously established methods (Agalioti et al. ...
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bioRxiv - Cell Biology 2022Quote: ... K562 CRISPRi cells with or without MICU1(GFP1-10) were nucleofected with a MTCH2 targeting guide in the pX458 backbone (Addgene plasmid # 48138) using the Lonza SF Cell Line 96-well Nucleofector Kit (V4SC-2096) ...
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bioRxiv - Molecular Biology 2020Quote: ... The Cre-containing plasmid was obtained from Addgene (pLM-CMV-R-Cre, Addgene #27546). A fragment encoding the CMV promoter and mCherry-T2A-Cre-WPRE was excised by NdeI and SacII (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... RA was PCR amplified from RA-NES (gift from R. Campbell, Addgene plasmid #61019) (Ding et al. ...
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bioRxiv - Cell Biology 2022Quote: ... GB was PCR amplified from GB-NES (gift from R. Campbell, Addgene plasmid #61017) (Ding et al. ...
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bioRxiv - Biochemistry 2021Quote: ... and R-CEPIA1er (a gift from Masamitsu Iino, Nihon University, Tokyo, Japan; Addgene plasmid #58216) (Suzuki et al. ...
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bioRxiv - Cell Biology 2022Quote: ... 2011) (pCMV-VSV-G and pLKO.1 puromycin were gifts from R. Weinberg through Addgene), using Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Plant Biology 2022Quote: ... Primers GFP-F-EcoRI and 3xNLS-R were used to obtain GFP-3xNLS from Addgene plasmid pEGFP-C1 EGFP-3xNLS ...
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bioRxiv - Neuroscience 2022Quote: ... with GCaMP6 (GCaMP6 medium, Addgene cat.40754) and the FeRIC channels ...
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bioRxiv - Microbiology 2020Quote: ... The UPS reporter construct Ub-R-GFP was obtained as a gift from Nico Dantuma (Addgene plasmid # 11938 ...
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bioRxiv - Cancer Biology 2021Quote: ... Reporter assays to assess RUNX1 transcriptional activity were done using the pMCSF-R-luc plasmid (Addgene plasmid #12420 ...
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bioRxiv - Neuroscience 2022Quote: ... The pDisplay-Gncp-iGluSnFR and pDisplay-R-iGluSnFR1 vectors were gifts from Dr Robert Campbell (Addgene plasmid #107337 and #107335 ...
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bioRxiv - Molecular Biology 2020Quote: 1×106 cells of WTC p42 were transfected with 5mg AAVS1-TALEN R plasmid (Addgene #59026), 5 μg AAVS1-TALEN L plasmid (Addgene #59025) ...
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bioRxiv - Cancer Biology 2021Quote: ... 2012) were cloned into the pLKO.1-Puro vector (a gift from R. Weinberg; Addgene #8453) to generate pLKO.1-Cys-TD and pLKO.1-Scr-TD ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids Ub-M-GFP and Ub-R-GFP were a gift from Nico Dantuma (Addgene #11939) (Dantuma et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... mNT-sgRNA-R: 5’-AAACCGCGGAGCCGAATACCTCGC-3’) were cloned into the lenti-sgRNA(MS2)-zeomycin backbone (Addgene #61427) using BsmBI ...
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bioRxiv - Cell Biology 2021Quote: ... The plasmid used for calcium imaging CMV-mito-R-GECO1 was a gift from Robert Campbell (Addgene plasmid #46021 ...
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bioRxiv - Bioengineering 2020Quote: ... either of the two cell types was subjected to Lipofectamine transfection with CMV-R-GECO1.2-mCherry (Addgene, Watertown ...
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bioRxiv - Cancer Biology 2021Quote: The M-CSF promoter reporter (pMCSF-R-luc Addgene plasmid # 12420; http://n2t.net/addgene:12420; RRID: Addgene_12420)(Zhang et al. ...
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bioRxiv - Neuroscience 2022Quote: ... AAVS1-TALEN-L and AAVS1-TALEN-R were gifts from Danwei Huangfu (Addgene plasmid # 59025 and 59026) (González et al. ...
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bioRxiv - Synthetic Biology 2020Quote: Ub-R-GFP was a gift from Nico Dantuma (Addgene plasmid # 11939; http://n2t.net/addgene:11939; RRID:Addgene_11939). Site directed mutagenesis was performed to generated Ub M-GFP and Ub-L-GFP using Q5 Hot Start High-Fidelity Polymerase New England Biolabs (Ipswich ...
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bioRxiv - Biochemistry 2021Quote: ... Ub-M–GFP (#11938) and Ub-R–GFP (#11939) plasmids described in [45] were purchased from Addgene. HA tagged USP5 (#22590 ...
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bioRxiv - Microbiology 2020Quote: ... The UPS reporter construct Ub-R-GFP was obtained as a gift from Nico Dantuma (Addgene plasmid # 11938; http://n2t.net/addgene:11938; RRID:Addgene_11938) and sub-cloned into the pKT3 plasmid using the NheI and SmaI sites ...
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bioRxiv - Cancer Biology 2021Quote: ... Reporter assays to assess RUNX1 transcriptional activity were done using the pMCSF-R-luc plasmid (Addgene plasmid #12420; http://n2t.net/addgene:12420; RRID:Addgene_12420) (Zhang et al. ...
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bioRxiv - Cell Biology 2020Quote: ... and nuclear (CMV-NLS-R-GECO) targeted calcium biosensors were gifts from Robert Campbell (Addgene #61244, and 32462) [40,41] ...
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bioRxiv - Cell Biology 2021Quote: ... the sequence coding for R-GECO1 including TorA-tag from pTorPE-R-GECO1 (a gift from Robert Campbell (Addgene plasmid #32465; http://n2t.net/addgene:32465; RRID:Addgene_32465), and (iv ...
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bioRxiv - Biophysics 2023Quote: ... generated by us in the Kiel center [51] using a modified version of the pHyVec1 plasmid which replaces the GFP sequence with a GCaMP6s sequence that was codon-optimized for Hydra (HyGCaMP6s was a gift from R. Yuste lab (Addgene plasmid # 102558; http://n2t.net/addgene:102558 ; RRID:Addgene_102558) [37]) ...
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bioRxiv - Molecular Biology 2023Quote: ... The TIS11B coding sequence was amplified from pcDNA3.1-puro-GFP-TIS11B using TIS11B MCP F and TIS11B MCP R primers and the TIAL1 coding sequence was PCR amplified from pFRT_TO_FlagHA_TIAL1 (Addgene 106090) using TIAL1 MCP F and TIAL1 MCP R primers.
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bioRxiv - Cancer Biology 2023Quote: ... PGL2 E-cad (108) Ebox Mut-Luc (kindly provided Dr. Eric R. Fearon, Addgene plasmids 19291 and 19290), and 0.025 μg of pRL-TK (Promega ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HEK-293 cells were transfected with two plasmid vectors CMV–R-GECO1 (a gift from Robert Campbell, Addgene plasmid #32444 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HEK-293 cells were transfected with two plasmid vectors CMV–R-GECO1 (a gift from Robert Campbell, Addgene plasmid #32444; http://n2t.net/addgene:32444; RRID:Addgene_32444) and PH(Akt)-Venus (a gift from Narasimhan Gautam ...
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bioRxiv - Cell Biology 2020Quote: ... CMV-R-GECO1 (32) and pDDRFP-A1B1-DEVD (75) were gifted by Robert Campbell (Addgene plasmid #32444 and #36294). pcDNA3-HA-human OCRL (76 ...
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bioRxiv - Molecular Biology 2021Quote: ... (i) 0.5 μg of AAVS1-TALEN-L and AAVS1-TALEN-R (gift from Dr. Danwei Huangfu, Addgene plasmid # 59025) as well as 2 μg of targeting plasmid were used for nucleofection of hPSC cells ...
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bioRxiv - Bioengineering 2020Quote: ... VPR was amplified by primer pair VPR-F/R (template source: pWalium20-10XUAS-3XFLAG-dCas9-VPR (Addgene No.: # 78897)(Lin et al. ...
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bioRxiv - Cell Biology 2021Quote: ... The plasmid used for calcium imaging CMV-mito-R-GECO1 was a gift from Robert Campbell (Addgene plasmid #46021; http://n2t.net/addgene: 46021; RRID: Addgene_46021).