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Citations for Addgene :
201 - 250 of 667 citations for MERS Coronavirus Spike Glycoprotein S1 His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: The mammalian expression plasmid containing SARS-CoV-2 HexaPro spike was obtained from Addgene (Addgene plasmid # 154754 ...
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bioRxiv - Immunology 2023Quote: ... Plasmids encoding ancestral and variant SARS-CoV-2 spike protein were purchased from Addgene (170442 ...
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bioRxiv - Neuroscience 2021Quote: ... E Dent include the plasmids EB3 tdTomato (Addgene #50708) and the plasmid encoding DsRed2 (Clontech) ...
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bioRxiv - Developmental Biology 2021Quote: ... The plasmid E[beta]C (Addgene cat. no. 24312) was used ...
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bioRxiv - Neuroscience 2023Quote: ... pAAV-Cag-Egfp-Apobec1-Yth (E-Yth, Addgene #209319), pAAV-Cag-Egfp-Apobec1-Ythmut(E-Ythmut ...
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bioRxiv - Neuroscience 2023Quote: ... pAAV-Cag-Apobec1-Ythmut-Egfp (Ythmut-E, Addgene #209323), pAAV-Cag-Apobec1-Egfp (Addgene #209324 ...
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bioRxiv - Neuroscience 2023Quote: ... pAAV-Cag-Egfp-Apobec1-Ythmut(E-Ythmut, Addgene #209320) and pAAV-Cag-Egfp-Apobec1 (Addgene #209321) ...
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bioRxiv - Genetics 2019Quote: Plasmid pF2-bio-His was obtained from Addgene (plasmid #52179) and human F2 cDNA was amplified using primers containing vector sequence homology (Table S1) ...
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bioRxiv - Cell Biology 2019Quote: His-tagged ubiquitin construct was obtained from Addgene (plasmid: #18712). siRNA#1 resistant RNF168-mCherry plasmid and RNF168 siRNAs were gift from Jiri Lucas (1) ...
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bioRxiv - Biochemistry 2021Quote: ... SARS-CoV-2 3xFlag-His-nsp5CS-nsp14 (Addgene ID 169163) and SARS-CoV-2 nsp14/nsp10-His-3xFlag (Addgene ID 169164 ...
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bioRxiv - Biochemistry 2021Quote: Plasmids SARS-CoV-2 nsp10-His-3xFlag (Addgene ID 169162), SARS-CoV-2 3xFlag-His-nsp5CS-nsp14 (Addgene ID 169163 ...
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bioRxiv - Biochemistry 2020Quote: ... and cloned into a His-SUMO backbone (Addgene Plasmid #37507) for expression ...
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bioRxiv - Biochemistry 2022Quote: ... The acp ORF was amplified from pET29a-acp-His (Addgene) using primers listed in Table S5 and inserted into SacI- and BamH1-digested pET28b-Smt3-His10 to give pET28b-Smt3-His10-acp.
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bioRxiv - Molecular Biology 2020Quote: ... pET-His-hRan-Q69L was acquired from AddGene (catalog #42048) and pGEX-TEV-hCRM1 (XPO1 ...
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bioRxiv - Bioengineering 2023Quote: ... were transformed with the plasmid pET28b-RfxCas13d-His (Addgene 141322), which contain the recombinant CasRx gene ...
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bioRxiv - Genomics 2023Quote: ... were transformed with pET-28b-Cas9-His (Addgene, no. 47327) by heat shock following the manufacturers’ instructions ...
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bioRxiv - Genetics 2023Quote: His-SIRT5 expression plasmid was obtained from Addgene (plasmid #25487). Site-directed mutagenesis of WT SIRT5 was performed using the QuickChange Lightning site-directed mutagenesis kit (#210518 ...
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bioRxiv - Biophysics 2021Quote: ... coli MscL-pET15b plasmid was purchased from Addgene (Addgene plasmid # 92418 ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli and derived from pET32a-TRXtag (Addgene #11516) (63) ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli CFPS were based on pJL1 (Addgene #69496) and some include an N-terminal Expression Tag (NET ...
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bioRxiv - Biochemistry 2022Quote: ... coli BL21(DE3) harboring pAC-BETAipi (Addgene # 53277), pCDFDuet-trAtCCD7-OsD27 (Addgene # 178285 ...
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bioRxiv - Molecular Biology 2022Quote: ... coli IYK-12 using pOSIP-CT (Addgene #45981) to create the strain IG-CR via clonetegration (54) ...
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bioRxiv - Bioengineering 2023Quote: ... Coli Type I Cascade system (Addgene #106270-106275) and Pae Type I Cascade System (Addgene #153942 and 153943) ...
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bioRxiv - Microbiology 2023Quote: ... coli S17-lamda-pir harboring pSAM-Bt (Addgene_112497 ...
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bioRxiv - Biophysics 2023Quote: ... coli using the plasmids K560CLM T324C (#24460, Addgene)18 ...
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bioRxiv - Biochemistry 2024Quote: ... coli Δlac cells (Addgene, Didovyk et al., 2017) transformed with plasmids encoding β-gal (wild-type or mutant ...
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bioRxiv - Cancer Biology 2021Quote: ... VRK1WT was further cloned into PLX305(C-TAG) (Addgene #91798) and VRK1WT ...
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bioRxiv - Genetics 2022Quote: ... The 3XFLAG-tag was cloned from pLP15 (Addgene plasmid #55180) with 5’ KpnI and 3’ EcoRI sites (primers LC127 and 128 ...
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bioRxiv - Neuroscience 2023Quote: ... respectively13 and produced AAV-CaMKIIα-GLuc-no tag (Addgene 149502) and AAV-CaMKIIα-GLuc-SERCaMP (Addgene 149503) ...
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bioRxiv - Developmental Biology 2023Quote: ... an amplified PCR product from pCAG-TAG (Addgene, Plasmid #26771) was cloned into the KpnI-NotI sites of pcDNA3.1-H2B mCherry-poly(A83 ...
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bioRxiv - Molecular Biology 2021Quote: ... Other plasmids used (for details see excel file S1): pLenti Lifeact-iRFP670 BlastR43 (Addgene Plasmid #84385); pGIZ-PuroR-IRES-GFP_shRNA anti Rab11a/b (Dharmacon) ...
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bioRxiv - Microbiology 2021Quote: ... pVAX1-SARS2-S1/S2-mut and pVAX1-SARS2-S_D614G) or VSV-G (pMD2.G Addgene #12259). The cell culture supernatants were harvested 24-72 h post transfection followed by addition of fresh media and again after 48-72h ...
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bioRxiv - Molecular Biology 2021Quote: ... or MGMT (Supplementary Table S1) were annealed and ligated into BsmBI-linearized lentiCRISPRv2 vector (Addgene, #52961). Other plasmids have been described previously (8,17) ...
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bioRxiv - Microbiology 2023Quote: pT7-S1/HER2nhd (no head domain) was constructed using pBacT7S1-T3D (Addgene, (Kobayashi et al., 2007) as a backbone plasmid ...
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bioRxiv - Biochemistry 2021Quote: ... 27μg pTT5LnX-WHCoV-St19 (SARS-CoV2 Spike) and 54 μg pHIV-Luc-ZsGreen (Addgene, US) using Lipofectamine 3000 transfection reagent (Thermo Fisher ...
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bioRxiv - Biophysics 2021Quote: The plasmid with cDNA encoding SARS-Cov-2 spike HexaPro (S) was obtained from Addgene. To express the S protein ...
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bioRxiv - Bioengineering 2021Quote: ... Lentivirus pseudotyped with the vesicular stomatitis virus spike G (pCMV-VSV-G; Addgene plasmid # 8454) was used as a positive control and the bald lentivirus having no coat was used as a negative control.
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bioRxiv - Cell Biology 2021Quote: ... and pCMV3 nCoV2019 Spike (D614 variant) C-FLAG Hygro was obtained from Addgene (Teddington, UK). Plasmid encoding for Spike G614 variant was generated using the pTwist EF1alpha nCoV-2019 S 2xStrep plasmid and the Q5 site-directed mutagenesis kit (New England BioLabs ...
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bioRxiv - Microbiology 2021Quote: ... and pcDNA3.1 expressing SARS-CoV-2 spike gene or VSV-G (pMD2.G, Addgene #12259) using VigoFect (Vigorous Biotechnology) ...
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bioRxiv - Genetics 2020Quote: ... we used an existing CMV-driven SARS-CoV-2 plasmid (pcDNA3.1-SARS2-Spike, Addgene 145032)(Shang et al. ...
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bioRxiv - Microbiology 2021Quote: ... and pcDNA3.1 expressing SARS-CoV-2 spike gene or VSV-G (pMD2.G (Addgene #12259)) using VigoFect (Vigorous Biotechnology) ...
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bioRxiv - Cancer Biology 2020Quote: ... Two crRNAs introduced into lentiviral vectors (pLentiCRISPR-E, Addgene #78852) which contains eSpCas9 and puroMYCin cassette.
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bioRxiv - Biophysics 2020Quote: ... containing N-terminal 6-His-WT-p53 (1-303) (Addgene, 24859), was used to express p53 ...
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bioRxiv - Developmental Biology 2021Quote: ... SpCas9 was expressed from pET28a-Cas9-His (Addgene plasmid number 98158) and purified in our lab ...
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bioRxiv - Biochemistry 2021Quote: ... and SARS-CoV-2 nsp14/nsp10-His-3xFlag (Addgene ID 169164) were expressed in baculovirus-infected Sf9 insect cells ...
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bioRxiv - Plant Biology 2024Quote: ... cells harboring the plasmid p2CT-His-MBP-Lbu_C2c2_WT (Addgene No. 83482) were cultivated with 800 ml autoinduction medium (Studier ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli BL21(DE3) was transformed with pAC-BETAipi (Addgene) and pCDFDuet-OsD27 ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli BL21(DE3) was transformed with pAC-BETAipi (Addgene) and pCDFDuet-OsD27-trAtCCD7 to generate E ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli S2060 cells were prepared containing pJC175e (Addgene #79219), a plasmid expressing pIII under control of the phage shock promoter32 ...
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bioRxiv - Biophysics 2020Quote: ... coli is pJCSUP35NM (1-253) (Addgene Plasmid number #1089). The over-expression of Sup35NM was induced using 1 M IPTG ...