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Citations for Addgene :
401 - 450 of 1062 citations for Dechlorane Plus Syn Bis Cyclopentene 13C10 99% 100 Ug Ml In Toluene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... 16 ug of plasmid were prepared for each plate at a ratio of 4:3:1 (sgRNA library: psPAX2(Addgene ID 12259): pMD2G(Addgene ID 12260)) ...
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bioRxiv - Biochemistry 2021Quote: ... N-terminal His6 plus glutathione S-transferase (GST; RRID:Addgene_29707); N-terminal His6 plus small ubiquitin-like modifier (SUMO ...
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bioRxiv - Genomics 2020Quote: ... plus packaging plasmids psPAX2 and pCMV-VSV-G (Addgene plasmids #52961 ...
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bioRxiv - Cell Biology 2019Quote: Wild-type or SURF4-deficient HEK293T cells that stably express EPO-eGFP and A1AT-mCherry were transfected with a plasmid expressing ERoxBFP (Addgene: 68126, a gift from Erik Snapp (99)) ...
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bioRxiv - Neuroscience 2021Quote: ... plus viral packaging helper plasmids pMD2.G (plasmid #12259, Addgene) and psPAX2 (plasmid #12260 ...
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bioRxiv - Biochemistry 2021Quote: ... or N-terminal His6 plus green fluorescent protein (GFP; RRID:Addgene_29716). Note that in the plasmid names for this clone the numbering of Syx residues was based on the Syx isoform from NCBI Reference Sequence NP_001036128.1 ...
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bioRxiv - Biochemistry 2021Quote: ... N-terminal His6 plus small ubiquitin-like modifier (SUMO; RRID:Addgene_29711); or N-terminal His6 plus green fluorescent protein (GFP ...
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Rhes protein transits from neuron to neuron and facilitates mutant huntingtin spreading in the brainbioRxiv - Neuroscience 2021Quote: ... The mCherry-HTT N171 18Q or 89Q were cloned in 3rd generation Lentiviral vector for bi-cistronic expression of EGFP and the gene of interest (Addgene, 24129) and transfected in HEK 293T cells along with packaging plasmids ...
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bioRxiv - Immunology 2020Quote: ... antisense: aaacCAGTGATGTCACCCGTGTGC) were hybridised and ligated into the Bsm BI site of pLentiGuide-Puro (gift from Feng Zhang, Addgene # 52963 (20)) ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 ng of CB1-GFP and 100 ng of Rab5-BFP (Addgene #49147) were mixed with 0.2 µL of lipofectamine 2000 following transfection method previously described then added to the wells ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 ng of CB1-GFP and 100 ng of R-GECO (Addgene #32444) were mixed with 0.2 µL of lipofectamine 2000 transfection reagent ...
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bioRxiv - Cancer Biology 2021Quote: ... plus pSPAX2 and pMD2.G (gifts from D. Trono; Addgene #12260 and #12259) using Lipofectamine 2000 ...
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bioRxiv - Microbiology 2023Quote: ... plus the pscALPS-hACE2 or -cACE2 (1 µg) or the empty pscALPS_puro (Addgene, 128504) vector as a negative control ...
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bioRxiv - Neuroscience 2022Quote: ... AAV2-hSyn-DIO-EGFP (Addgene; 100 nl) (Figure 1E ...
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bioRxiv - Molecular Biology 2023Quote: ... A BRCA1-100 construct from Addgene (36) was subsequently introduced into the His- MBP-TEV-BARD126-777 vector by Gibson assembly in frame between the TEV site and BARD1 (37 ...
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bioRxiv - Biochemistry 2023Quote: ... and 100 ng pMD2.G (Addgene, #12259) with TransIT-Lenti (Mirus ...
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bioRxiv - Cancer Biology 2022Quote: Lentiviral transfer plasmid plus packaging plasmids psPAX2 (gift from Didier Trono, Addgene Plasmid #12260, RRID:Addgene_12260) and pCMV-VSV-G (gift from Bob Weinberg ...
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bioRxiv - Cancer Biology 2022Quote: Lentiviral transfer plasmid plus packaging plasmids psPAX2 (gift from Didier Trono, Addgene Plasmid #12260, RRID:Addgene_12260) and pCMV-VSV-G (gift from Bob Weinberg ...
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bioRxiv - Neuroscience 2023Quote: ... and 60-120nl AAV2-Ef1a-fDIO-mCherry (2.65×1013 vg/ml, 1.84×1013 vg/ml and 1.1×1013 vg/ml, respectively; Addgene). For monosynaptic retrograde rabies experiments in Foxp2cre+/- mice we injected unilaterally into the MeA 250-500nl of mixed AAV1-CA-Flex-RG and AAV8-Ef1-Flex-TVA-mCherry (1:1 ...
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bioRxiv - Microbiology 2021Quote: ... 100 ng of plasmid plRL19 (Addgene plasmid #163634) was also added ...
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bioRxiv - Neuroscience 2019Quote: ... and 100 µg/µL pCAG-TVA800(Addgene 15788).
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bioRxiv - Neuroscience 2022Quote: ... or AAV2-hSyn-DIO-EGFP (Addgene; 100 nl) (Figure 3A ...
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bioRxiv - Neuroscience 2022Quote: ... 100 nL injected (both from Addgene, Massachusetts, US). Following injection ...
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bioRxiv - Microbiology 2022Quote: ... 100 ng of plasmid plRL19 (Addgene plasmid #163634) was also added ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 ng of the EPAC sensor (Addgene #61622) were mixed with 0.2 µL of lipofectamine 2000 following the transfection method previously described ...
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bioRxiv - Cell Biology 2024Quote: ... PEmax or PE-Plus were constructed by replacing Cas9 in the Hygro-Cas9 donor plasmid (Addgene #86883) with the respective PE2 ...
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bioRxiv - Bioengineering 2021Quote: ... 5 uL of serum-free media was added to 200 ng of plasmid DNA (100 ng CAG-nanobody-TagBFP plasmid and 100 ng CAG-dsRed plasmid (Addgene plasmid 11151) (Matsuda and Cepko ...
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bioRxiv - Neuroscience 2023Quote: ... we injected 100 nL of pAAV-FLEX-tdTomato (Addgene, 28306-AAVrg ...
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bioRxiv - Cancer Biology 2022Quote: ... Complementary oligonucleotides encoding sgRNA sequences plus appropriate overhangs were annealed and cloned into BsmBI-digested sgOpti (Addgene #85681).
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bioRxiv - Physiology 2023Quote: ... plus a five-amino-acid linker plus a C-terminal flag tag was cloned into the pENN.AAV.CB7.CI.pm20d1flag.WPRE.rBG vector (Addgene plasmid no. 132682). AAV8-GFP (pENN.AAV.CB7.CI.eGFP.WPRE.rBG),used as control ...
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bioRxiv - Neuroscience 2023Quote: ... 150nl of AAV2-hSyn-DIO-hM3D(Gq)-mcherry or 150-600nl of AAV2-hSyn-DIO-mCherry (3×1012 vg/ml, 5.1×1012 vg/ml and 5.6×1012 vg/ml, respectively; Addgene and UNC Vector Core) into the MeA of Foxp2cre+/- mice ...
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bioRxiv - Neuroscience 2020Quote: ... we injected 100 μL of AAV1.Syn.Flex.GCaMP7b (Addgene, #104493-AAV1) bilaterally into visual area PM (−3.3 mm posterior ...
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bioRxiv - Neuroscience 2020Quote: ... 100 nl of AAV1-CAG-tdTomato-WPRE-SV40 (Addgene, 105554) was injected in POm (AP −2.06 ...
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bioRxiv - Biochemistry 2021Quote: ... by cloning our codon optimized Syx gene into vectors containing the following tags that are cleavable with tobacco etch virus (TEV) protease: N-terminal His6 plus maltose binding protein (MBP; RRID:Addgene_29708); C-terminal MBP plus His6 (Addgene_37237) ...
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bioRxiv - Microbiology 2020Quote: ... a 6.6 kb genomic fragment of nhr-49 gene (comprising of 4.4 kb coding region covering all nhr-49 transcripts plus 2.2 kb sequence upstream of ATG) was cloned into the GFP expression vector pPD95.77 (Addgene #1495), as reported previously (Ratnappan et al. ...
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bioRxiv - Neuroscience 2022Quote: ... retrograde (1E12 GC/mL, Addgene 55637 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were transduced the next day with 1000 ng of plasmid DNA plus 250 ng pMD2.G (Cat# 12259, Addgene) and 750 ng psPax2 (Cat# 12260 ...
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bioRxiv - Cancer Biology 2020Quote: ... modified to express human histone H2B tagged at the N-terminus with GFP (pLVX-myc-EmGFP-H2B) plus psPAX2 and pMD2.G (gifts from Didier Trono, Addgene) using 16.6 mM CaCl2 in DMEM supplemented with 10% Hyclone™ serum (GE Healthcare ...
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bioRxiv - Systems Biology 2021Quote: ... HEK293FT cells were transfected with one of the three destination vectors plus a lentiviral packaging vector (psPAX2, Addgene plasmid #12260) and a VSV-G envelope expressing vector (pMD2.G ...
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bioRxiv - Immunology 2020Quote: ... with lentiviral packaging vectors pCAG-eco and psPAX.2 plus empty pLKO.1 control (EV) with a puromycin selection cassette (all obtained from Addgene) or a shRNA containing pLKO.1 targeting DGAT1 (GE Dharmacon CAT# RMM4534-EG13350 - TRCN0000124791) ...
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bioRxiv - Immunology 2022Quote: ... with lentiviral packaging vectors pCAG-eco and psPAX.2 plus empty pLKO.1 control (EV) with a puromycin selection cassette (all obtained from Addgene) or a shRNA containing pLKO.1 targeting Cpt1a (GE Dharmacon CAT# RMM3981-201819967 – TRCN0000110596 ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 2.5ul PLUS reagent with 1000 ng of reporter donor plasmid and 500 ng of each TALEN-L (Addgene 35431) and TALEN-R (Addgene 35432 ...
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bioRxiv - Neuroscience 2023Quote: ... Janelia) or AAVretro_Syn_GCamp7f (1.0e13 GC/ml, lot #21720; 1.2e12GC/ml, lot #v52598, Addgene 104488-AAVrg) in the right dorsolateral striatum (DLS ...
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bioRxiv - Cell Biology 2020Quote: 100 ng of human sgRNA library Brunello in lentiCRISPRv2 (Addgene, #73179) was transformed into electrocompetent Endura cells (#60242 ...
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bioRxiv - Neuroscience 2021Quote: ... 100-200 nl of AAV8-hSyn-DIO-hM4D(Gi)-mCherry (Addgene) were injected (DV −3.6 mm ...
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bioRxiv - Neuroscience 2022Quote: ... and 100-200 nl cre-dependent tdTomato (AAV2/1.FLEX.tdTomato.WPRE.SV40, Addgene), and some cases ...
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bioRxiv - Neuroscience 2023Quote: ... we injected 3×100 nl AAV5.Ef1a.DIO.eYFP (Penn Core, Addgene: 27056) into the mPFC (AP/ML/DV coordinates 2.2 ...
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bioRxiv - Cell Biology 2020Quote: ... HeLa cells were transfected with a plasmid that expresses RNAi-resistant ch-TOG:GFP plus a hairpin RNA (sh ch-TOG) to knockdown endogenous protein and minimize overexpression (Addgene ID# 69113). HeLa cells stably expressing GFP:tubulin were previously generated (van Oostende Triplet et al. ...
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bioRxiv - Physiology 2023Quote: ... Tracking of the plus-end side of the microtubules was performed by transiently transfecting the cells with EB3-tdTomato (Addgene #50708) 24 hours after plating following the protocol described in the previous section ...
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bioRxiv - Cell Biology 2020Quote: ... 1.35 µg/ml pPAX2 (Addgene: 12260) and 0.5/ml µg pMD2.G (Addgene ...