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Citations for Addgene :
301 - 350 of 1062 citations for Dechlorane Plus Syn Bis Cyclopentene 13C10 99% 100 Ug Ml In Toluene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... pGP-AAV-syn-jGCaMP7f-WPRE was a gift from Douglas Kim & GENIE Project (Addgene viral prep #104488-AAV9). Trace amounts of Fast-Green (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... 100-200 nL of rAAV2-retro jGCaMP7s (~1e13 gc/mL, Addgene) was injected at a rate of 70 nL·min-1 using a Microinject system (World Precision Instruments) ...
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bioRxiv - Cancer Biology 2021Quote: ... 5ug of plasmid library was co-transfected with four ug PsPAX2 (Addgene #12260) and one ug pMD2.G (Addgene #12259 ...
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bioRxiv - Neuroscience 2021Quote: ... Each transfection condition contained 0.5 ug of a plasmid encoding GCaMP6s (Addgene #40753) and 1.5 ug of the plasmid encoding the appropriate olfactory receptor ...
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bioRxiv - Neuroscience 2021Quote: ... We bilaterally injected 368 nl of AAV2/9 hSyn.hChR2(H134R).eYFP.WPRE.hGH (UPenn Vector Core) or AAV2/9 CaMKII.ArchT-GFP (UNC Vector Core) or pGP-AAV-syn-jGCaMP7f-WPRE (Addgene) to the LEC or MEC ...
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bioRxiv - Neuroscience 2019Quote: ... Virus injections per mouse pup were in a total volume of 4μL of AAV9-syn-GCaMP6s (Addgene, MA, USA) with a titer of 1Χ1013 vg/mL ...
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bioRxiv - Neuroscience 2022Quote: Ucn3-cre animals were injected with a cocktail of 150 nL of AAV-syn-jGCaMP7f-WPRE (Addgene 104488-AAV9) and 225 nL of AAV-hSyn-DIO-hM4D(Gi)-mCherry into PeFA (ML ...
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bioRxiv - Neuroscience 2021Quote: ... a glass pipette (0.1-0.2 mm diameter at the tip) containing the pAAV-Syn-Chronos-GFP (Addgene #59170-AAV1) or AAV1-hSyn-Cre (Addgene #105553-AAV1 ...
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bioRxiv - Neuroscience 2020Quote: ... to measure BLA ACh levels (Fig. 2A-E + S2.1-S2.2) or 0.5 µL of AAV1 Syn-FLEX-GCaMP6s-WPRE-SV40 (Addgene, Watertown, MA) to measure BLA principal cell calcium dynamics (Fig ...
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bioRxiv - Neuroscience 2021Quote: ... Adeno-associated virus containing the GCaMP7f gene (pGP-AAV9-syn-FLEX-jGCaMP7f-WPRE, 104488-AAV9, Addgene, Watertown, MA, USA) was loaded into a glass micropipette with a tip diameter of 40–50 µm attached to a Nanoject II injection system (Drummond Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... mice (P40 – P50) were injected with 200 nL of AAV5-Syn-GFP in the rostral migratory stream (RMS, Addgene), using the following stereotaxic coordinates (in mm) ...
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bioRxiv - Neuroscience 2022Quote: ... Adeno-associated virus for expressing jGCaMP6f or jGCaMP7f under the synapsin-1 promoter (AAV1-syn-jjGCaMP6f-WPRE-SV40, Addgene, 100837 ...
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bioRxiv - Neuroscience 2022Quote: ... Excitatory specificity was ensured using either a cre dependent AAV (Syn-ChroME Addgene 170161; or CAG-ChroME2s Addgene 170163) in an excitatory specific cre line (Emx1-Cre ...
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bioRxiv - Neuroscience 2022Quote: ... Excitatory specificity was ensured using either a cre dependent AAV (Syn-ChroME Addgene 170161; or CAG-ChroME2s Addgene 170163) in an excitatory specific cre line (Emx1-Cre ...
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bioRxiv - Neuroscience 2023Quote: ... was made similarly but with the tricistronic open reading frame from pAAV-syn-FLEX-splitTVA-EGFP-tTA (Addgene 100798) except for the use of FRT sites instead of lox ones (and with a 2-bp frameshift of the in-frame FRT site to prevent creation of a premature stop codon) ...
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bioRxiv - Neuroscience 2023Quote: ... pCAG-NES-jRGECO1a was generated by subcloning a NES-jRGECO1a insert (from pAAV-Syn-NES-jRGECO1a-WPRE, Addgene #100854) into a pCAG backbone ...
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Somatostatin interneurons control the timing of developmental desynchronization in cortical networksbioRxiv - Neuroscience 2023Quote: Pups were injected at birth (P0) with a viral cocktail containing pAAV1-syn-GcaMP6s-WPRE-SV40 (Addgene, 100843-AAV1) and pAAV1-syn-GcaMP6s-Flex WPRE-SV40 (Addgene ...
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bioRxiv - Neuroscience 2024Quote: ... pGP-AAV-Syn-FLEX-jGCaMP8s-WPRE was developed from the GENIE Project (Zhang et al., 2023) (162377-AAVrg, Addgene). pAAV-Syn-FLEX-GCaMP6s-WPRE-SV40 was developed from Douglas Kim & GENIE Project (Chen et al. ...
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bioRxiv - Genomics 2023Quote: ... 2.5 ug of DNA (the pegRNA plasmid and the pCMV-PE2 plasmid (Addgene #132775) at a 1:2 ratio by mass of pegRNA plasmid:PE2) ...
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bioRxiv - Neuroscience 2021Quote: ... A pulled glass micropipette attached to a nanoinjector (MO-10, Narishige) was used to inject 400nL of pGP-AAV9-syn-FLEX-jGCaMP7s-WPRE (Addgene) virus at a depth of 4.8mm DV at a rate of 100 nL/min ...
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bioRxiv - Biochemistry 2020Quote: The plasmid encoding WT-α-syn was cloned into a pAAV vector under the control of a CMV promoter (Addgene plasmid #36055 ...
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bioRxiv - Neuroscience 2022Quote: ... cultures were infected at 5 days in vitro (DIV) with titer-matched viruses using pAAV-Syn-ChrimsonR-tdT (Addgene #59171) and pAAV2.5-TH-GFP (Addgene #80336) ...
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bioRxiv - Neuroscience 2019Quote: ... 2011) for electron microscopy experiments and AAV1/2.Syn-hChR2(H134R)-EYFP (Received as a gift from Karl Deisseroth, Addgene plasmid # 26973 ...
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bioRxiv - Neuroscience 2020Quote: In order to express GCaMP6f in cerebellar MLIs we injected the vermis of the cerebellum in three adult PV-Cre mice with 2.0 μl of AAV1-Syn-Flex-GCaMP6f (Addgene, USA) (6.8 mm posterior to Bregma ...
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bioRxiv - Neuroscience 2021Quote: Cre-dependent adeno-associated virus (AAV; serotypes 5 or 9) was used to express GCaMP6s (AAV5-Syn-Flex-GCaMP6s, Addgene), or GCaMP7f (AAV9-Syn-Flex-jGCaMP7f-WPRE ...
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bioRxiv - Neuroscience 2022Quote: ... Vector Core and BrianVTA (China) unless described otherwise: rAAV9-CaMKIIα-hChR2(H134R)-eYFP (26969) and rAAV5-Syn-Flex-GCaMP6f-WPRE (100833) from Addgene; rAAV5-CaMKIIα-mCherry-Cre ...
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bioRxiv - Neuroscience 2023Quote: ... were injected in the right IC with the recombinant adeno-associated virus (rAAV) rAAV1/ Syn-Flex-Chronos-GFP (Lot # AV6551B, UNC Vector Core, Addgene # 62725 ...
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bioRxiv - Neuroscience 2023Quote: A glass capillary pulled to a fine tip was pre-loaded with a retrograde virus expressing both GFP and channelrhodopsin (ChR2) (pAAV-Syn-ChR2(H134R)-GFP (Addgene, 7 x 1012 vg/ml in PBS/NaCl/pluronic acid F-68 ...
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bioRxiv - Neuroscience 2023Quote: ... A similar approach was used to target SpVc where we injected 500 nL of pAAV5-Syn-Chronos-GFP (Addgene # 59170) at a depth of 0.5 mm ...
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bioRxiv - Neuroscience 2023Quote: For Synaptic plasticity experiments C57Bl/6-Jax mice were injected with AAV-Chronos (pAAV-Syn-Chronos-GFP, Addgene 59170-AAV5) virus in area S2 at locations and concentrations given in Table M2.
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bioRxiv - Neuroscience 2023Quote: ... were injected in the right IC with either rAAV1/ Syn-Flex-Chronos-GFP or rAAV9-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 (Addgene # 108912 ...
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bioRxiv - Neuroscience 2023Quote: ... into the DMS (0.3 µl) and of an AAV encoding the cre-dependent genetically encoded calcium indicator GCaMP8s (AAV9-Syn-FLEX-GcAMP8s-GFP, Addgene) into either the CeA or BLA (0.1-0.2 µl) ...
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bioRxiv - Neuroscience 2023Quote: ... and AAV encoding the cre-inducible inhibitory designer receptor human M4 muscarinic receptor (hM4DGi; AAV2-Syn-DIO-hM4Di-mCherry; Addgene) or fluorophore control (AAV2-Syn-DIO-mCherry ...
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bioRxiv - Neuroscience 2023Quote: ... mice received unilateral infusion (left/right hemisphere counterbalanced across subjects within each group) of a retrogradely trafficked AAV encoding cre-recombinase (AAVrg-Syn-Cre-P2A-dTomato, Addgene) into the DMS (0.3 µl ...
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bioRxiv - Neuroscience 2023Quote: ... For retrograde tracing a combination of two helper AAVs (AAV1-TREtight-mTagBFP2-B19G and AAV1-syn-FLEX-splitTVA-EGFP-tTA, both Addgene) and pseudotyped rabies (EnvA G-Deleted Rabies-mCherry ...
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bioRxiv - Neuroscience 2024Quote: ... all mice were unilaterally injected with 800nL of AAV9-syn-GCaMP8m-WPRE at a titer of 1.2e12 (Addgene virus #162375) in the RSC ...
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Real-time activity of dynorphin-expressing neurons in mouse central amygdala during alcohol drinkingbioRxiv - Neuroscience 2024Quote: Mice underwent unilateral surgical delivery of a Cre-dependent GCaMP7f calcium sensor into the CeA (pGP-AAV1-syn-FLEX-jGCaMP7f-WPRE, a gift from Douglas Kim & GENIE Project, Addgene viral prep # 104492-AAV1 ...
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bioRxiv - Neuroscience 2024Quote: The AAV carrying the gene for a genetically encoded calcium indicator (pGP-AAV1-syn-FLEX-jGCaMP7f-WPRE, Addgene plasmid #104492) was injected unilaterally into the right mPFC (1.5 mm ventral from brain surface ...
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bioRxiv - Neuroscience 2024Quote: ... i3Ns and iDAs were transduced with lentivirus expressing GCaMP7s driven by the Synapsin promoter (pCDH.rSyn.jGCaMP7s-cloned from pGP-AAV-syn-jGCaMP7s-WPRE, Addgene plasmid #104487) two days before live cell imaging ...
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bioRxiv - Neuroscience 2024Quote: ... 200 µg of pH GT1-ademo/dF6 helper and 70 µg of pGP-AAV-syn-jGCaMP7b-WPRE (Plasmid Number 104489, Addgene)56 and mix with 18 ml OptiMEM (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2022Quote: ... we prepared the vector backbone by incubating 5 ug of CROPseq-Guide-Puro (Addgene #86708) for 2 h at 37 °C with 2 ul of FastDigest Mph1103I (ThermoFisher cat ...
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bioRxiv - Neuroscience 2022Quote: ... One microliter of endo-free purified (2 ug/ul) pCAG-EGFP plasmid (Addgene, cat# 89684) mixed with 0.05% Fast Green (Sigma ...
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bioRxiv - Neuroscience 2020Quote: Raphe and RTN neurons - AAV-9: pGP-AAV-syn-GCaMP6s-WPRE.4.641 at a titre of 1×1013 GC·ml- 1 (Addgene, Watertown, MA, USA);
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bioRxiv - Genetics 2021Quote: ... for the deletion step using a one-step cloning reaction as described by the Thomas lab 25 The same protocol with the minor modification of replacing the second oligo with water during ligation was used to clone the syn-sgRNAs into pX459 (Addgene #62988). A cloning mixture volume of 5μL or 10μL was then transformed into 25μL or 50μL ...
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bioRxiv - Neuroscience 2020Quote: ... we used NheI and HindII to insert it into the pAAV backbone of pAAV-syn-ChR2ETTC (Bernd et al., 2011, ref Addgene 101361). To create TubuTag ...
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bioRxiv - Neuroscience 2022Quote: ... was injected unilaterally in the A13 while for DREADD experiments viruses for reversibly activating (AVV8-Syn-hM3D-Gq-mCherry; Addgene # 44361), inhibiting (AVV8-hSyn-hM4D-Gi-mCherry ...
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bioRxiv - Neuroscience 2021Quote: ... 100 nl of AAV1-Syn-GCaMP6f-WPRE-SV40 (University of Pennsylvania Vector Core) was injected alone or along with AAV1-FLEX-tdTomato (Addgene, #28306) 1.7 mm below dura at a rate of 100 nl/min (KD Scientific ...
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bioRxiv - Neuroscience 2020Quote: pFL - AAV-9: pGP-AAV-syn-GCaMP6f-WPRE.24.693 at a titre of 1×1013 GC·ml-1 (Addgene, Watertown, MA, USA).
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bioRxiv - Neuroscience 2022Quote: ... Mouse WT or A53T α-syn cDNAs (gifted by Dr. Eliezer Masliah, National Institute on Aging) was cloned into pmEos3.2-N1 vector (from Addgene, Cat#54525) to generate the α-syn -mEos3.2/α-syn A53T-mEos3.2 plasmid.
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bioRxiv - Neuroscience 2023Quote: ... or VIP-Cre80 mouse lines in combination with the Cre-inducible viral expression of GCaMP6f in the ACC (AAV1/9-SYN-FLEX-GCaMP6f; Addgene, #100833).