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Citations for Addgene :
401 - 415 of 415 citations for DNA Sequencer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The hybridized double-stranded (ghCOL7A1-S + ghCOL7A1-A) DNA fragment cloned into the Esp3I (BsmBI) restriction sites of the LentiCRISPRv2GFP (Addgene plasmid # 82416) using a simultaneous digestion-ligation reaction as described previously (16) ...
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bioRxiv - Biochemistry 2022Quote: ... The following plasmids used for DNA damage experiments in HeLa Tet-On cells were gifts from Kathleen Burns33: pDA007 (Addgene plasmid # 131380), pDA025 (Addgene plasmid # 131384) ...
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bioRxiv - Cell Biology 2023Quote: ... self-complementary single-stranded DNA oligos (Supp Table S1) were annealed and cloned into AgeI/EcoR1 sites of Tet-pLKO-puro vector (Addgene plasmid # 21915). MSI2 ORF (NM_138962.2 ...
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bioRxiv - Biochemistry 2023Quote: ... fluorophore labelled ‘601’ DNA was generated using large-scale PCR with Phusion polymerase (produced in-house) from a pGEM- 3z/601 plasmid containing one copy of ‘601’ DNA (gift from J. Widom, Addgene plasmid #26656)(Lowary & Widom ...
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bioRxiv - Developmental Biology 2024Quote: DNA templates for CRISPR gRNAs were synthesized using plasmid pX335-U6- Chimeric_BB-CBh-hSpCas9n(D10A) (Addgene, plasmid #42335; Cong et al., 2013) containing the gRNA scaffold ...
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bioRxiv - Developmental Biology 2023Quote: Upstream cis-regulatory elements were amplified from wildtype zebrafish genomic DNA and cloned into the e1b-eGFP-Tol2 vector (available from Addgene, Plasmid #37845) by restriction cloning at the XhoI and BglII sites ...
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bioRxiv - Neuroscience 2020Quote: ... Oligonucleotides encoding guide sequences are purchased from Integrated DNA Technologies (IDT) and cloned individually into BbsI fragment of pX458 (Addgene plasmid 48138(54)) ...
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bioRxiv - Plant Biology 2022Quote: ... while the full-length coding sequence of HlMYB7 was cloned into the prey vector pGADT7-GW [DNA-binding domain (BD)] (Addgene Inc, MA, USA) using the In-Fusion Snap Assembly Kit (Takara Bio) ...
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bioRxiv - Neuroscience 2024Quote: Vectors coding the dCas9-DNMT3ACD-DNMT3LCD-3xFLAG fusion gene (contains the enzymatic group of the DNA methyltransferase DNMT3) and the control plasmid pCMV-dCas9-mD3A (#78257, Addgene, Watertown, MA, USA) were used as described in 14,22 ...
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bioRxiv - Genetics 2022Quote: ... The resulting modified LexA (mLexA) chimeras were combined through DNA assembly (Gibson et al. 2009) with the following components: eLOV (Addgene # 92213, Alice Ting lab) (Wang et al ...
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bioRxiv - Microbiology 2022Quote: sgRNA sequences against gene targets were designed using CRISPick(56) and the corresponding DNA oligos (IDT) were annealed and ligated into pX458 (Addgene #48138, gift from Feng Zhang)(57) ...
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bioRxiv - Cell Biology 2022Quote: ... that had been amplified from the N2 genomic DNA were inserted into the ClaI-SpeI-cleaved pDD282 (a gift from Bob Goldstein, Addgene #66823, Dickinson et al., 2015) by Gibson assembly ...
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bioRxiv - Molecular Biology 2023Quote: Single guide RNA (sgRNA) oligonucleotides (Integrated DNA Technologies) were cloned into lentiviral expression vectors pKLV-U6gRNA(BbsI)-PGKpuro2ABFP (Addgene #50946, a gift from Kosuke Yusa)67 as described ...
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bioRxiv - Systems Biology 2022Quote: DNA oligos (Integrated DNA Technologies) containing gRNA sequences complementary to MORC3 or SETDB1 genomic loci were annealed and ligated into pX458 (Addgene #48138, gift from Feng Zhang). A549 cells were transfected with pX458 constructs using Lipofectamine 3000 and two days later GFP-positive cells were single-cell sorted into 96-well plates containing complete media ...
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bioRxiv - Molecular Biology 2023Quote: The control vector pEGFP was generated by Gibson assembly 106 of the following DNA fragments: the EF-1 alpha promoter (amplified from pEF1a-mRor2WT, Addgene #2261, a gift from Roel Nusse 107) and the EGFP-ori-AmpR fragment (amplified from pCMV_ABEmax_P2A_GFP ...