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Citations for Addgene :
301 - 350 of 415 citations for DNA Sequencer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... E15 wild-type embryos were unipolar injected into the ventricle using a pulled glass micropipette containing a DNA solution (1 μg/μl CAG-GFP plasmid (11150, Addgene) with 0.03% Fast Green in PBS) ...
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bioRxiv - Microbiology 2023Quote: ... and lentiviral particles expressing human TMPRSS2 (selection with 1 mg/ml G418). The TMPRSS2 (cat. 145843) and ACE2 (cat. 145839) lentiviral DNA clones (23) were purchased from Addgene and transfected into HEK293T cells together with pSPAX2 (Addgene ...
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bioRxiv - Neuroscience 2022Quote: ... the complementary DNA (cDNA) for each iMN factor (Ngn2, Lhx3, Isl1, NeuroD1, Ascl1, Brn2 and Myt1l) was purchased from Addgene and cloned into the pMXs retroviral expression vector using Gateway cloning technology (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... 2-6×105 cells in a 35 mM well were transfected with 0.4ug of DNA encoding a puro resistance plasmid (pCoPURO, Addgene #17533) using Effectene Transfection Reagent (Qiagen) ...
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bioRxiv - Developmental Biology 2023Quote: ... we aimed to substitute the endogenous Cas9 cassette by targeting the Cas9 protein to the Cas9 DNA flanking regions either with a dCas9-VP64 donor sequence from Addgene plasmid 61425 or a dCas9-KRAB donor ...
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bioRxiv - Bioengineering 2023Quote: A DNA sequence encoding the CD47 Ig-like domain (Gln19 – Ser135) was cloned into the pCTCON2 yeast-surface display vector (Addgene) using the NheI and BamHI sites ...
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bioRxiv - Biophysics 2023Quote: ... Plasmid DNA for expressing CRY2olig tagged with mCherry at its C-terminus was obtained from Addgene (#60032; Watertown, MA, USA). The plasmid (1.0 µg/dish ...
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bioRxiv - Genomics 2023Quote: ... The original CRISPRoff plasmid DNA (except the region encoding TagBFP) and mScarletI cDNA from the pmScarlet-i_C1 plasmid (Addgene # 85044) were amplified by polymerase chain reaction (PCR ...
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bioRxiv - Genomics 2023Quote: ... the DNA sequence of KRAB repressor domain was amplified by PCR from the pHAGE TRE dCas9-KRAB (Addgene plasmid #50917) and cloned in frame into the PB-TRE-dCas9-VPR backbone (Addgene plasmid #63800 ...
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bioRxiv - Immunology 2023Quote: DNA inserts containing the coding DNA sequence (CDS) for each wild type gene were generated by restriction digestion previous amplification from plasmids obtained from Addgene (hMX1 ...
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bioRxiv - Biochemistry 2022Quote: ... The DNA fragments were purified and cloned into a pOPINJ vector (a kind gift from Ray Owens, Addgene plasmid 26045) to encode a 3C-cleavable His6-GST-fusion using In-Fusion enzyme (Clontech ...
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bioRxiv - Cell Biology 2022Quote: ... The sgRNA expression vectors were generated by cloning appropriate DNA oligonucleotides (Suppl. Table 8) in the BbsI restriction sites of pX459 (#62988, Addgene). In brief ...
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bioRxiv - Cell Biology 2023Quote: ... The DNA fragment encoding integrin β4 was amplified from pcDNA3.1/Myc-His beta4 (a gift from Filippo Giancotti, Addgene #16039), and then inserted into the pEGFP-N1 vector for the expression of β4-GFP ...
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bioRxiv - Synthetic Biology 2022Quote: ... cells were co-transfected using Lipofectamine 3000 and 15μg of DNA encoding for viral protein (pCMV14-3X-Flag-SARS-CoV-2 S was a gift from Zhaohui Qian - Addgene plasmid # 145780 ...
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bioRxiv - Neuroscience 2023Quote: ... we inserted a DNA cassette encoding TeTxLC-T2A-EYFP (VectorBuilder) or mCherry-T2A-hM4Dinrxn (Addgene, #52523, donated by Scott Sternson) into the plasmid ...
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bioRxiv - Microbiology 2023Quote: ... gRNA oligos + PAM sequence from Integrated DNA Technologies (IDT) to clone into the lentiCRISPR v2 that was a gift from Feng Zhang (Addgene plasmid # 52961 ...
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bioRxiv - Cell Biology 2023Quote: ... In vitro target specific gRNA cleavage activity was validated by transfecting N2A cells with PCR amplified gRNA gblock and Cas9 plasmid DNA (px330, Addgene) using ROCHE Xtremegene HP ...
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bioRxiv - Biochemistry 2023Quote: E.coli codon optimized gBlocks encoding CAHS D and its mutant proteins used in this study were synthesized by (Integrated DNA Technologies) and cloned into pET-28 b (+) vector (Addgene) for bacterial expression ...
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bioRxiv - Developmental Biology 2023Quote: ... expressing a dominant negative variant of the rol-6 gene and an empty vector pSK (up to 200 ng μl-1 of DNA) (Addgene) using standard methods (42) ...
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bioRxiv - Cell Biology 2023Quote: ... The sgRNA expression vectors were generated by cloning appropriate DNA oligonucleotides (Supplementary Table 2) into the BbsI restriction sites of the pX459 vector (#62988, Addgene). An empty pX459 vector was used to generate matching control cell lines ...
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bioRxiv - Biochemistry 2023Quote: ... the open reading frame of QBP was amplified from E.coli K12 DNA and cloned into bacterial expression vector pET28a-LIC (Addgene #26094). Transformed E.coli BL21 (DE3) ...
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bioRxiv - Neuroscience 2023Quote: ... UBA5) were generated as described above with the following modifications: third-generation packaging DNA mix containing pRSV-REV (Addgene #12253), pMDLg/pRRE (Addgene #12251) ...
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bioRxiv - Immunology 2024Quote: ... targeting CYLD was cloned by annealing two DNA oligos (forward, 5’-GTGGTCAAGGTTTCACTGAC-3’; reverse, 5’-GTCAGTGAAACCTTGACCAC-3’) and ligating into lentiCRISPER v2 (52961, Addgene) plasmids ...
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Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest diseasebioRxiv - Plant Biology 2024Quote: For dsRNA vectors assembly, constructs sequences were synthesized in Integrated DNA Technologies (IDT, Coralville, IA, USA) and cloned into the T777T plasmid (Addgene plasmid # 113082 ...
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bioRxiv - Neuroscience 2024Quote: ... the DNA mixture consisted in 20 μg of specific DNA and 10 μg each of psPax2 and pMD2.G plasmids (from Dr. Didier Trono, Addgene) and 250 mM CaCl2 (in a final volume of 500 ul ...
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bioRxiv - Cancer Biology 2024Quote: ... two sgRNAs were synthesized together with bovine U6 promoter as gene blocks (Integrated DNA Technologies) and cloned using Gibson assembly into LRG2.1T (Addgene, 65656). All inserts were verified by Sanger sequencing (Eurofins Genomics) ...
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bioRxiv - Genetics 2024Quote: ... The two complementary DNA oligos of each sgRNA target were annealed and ligated to the pUC57-sgRNA plasmid (Addgene, USA) for cloning ...
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bioRxiv - Molecular Biology 2020Quote: ... Rpb7 was PCR amplified from HEK293T cDNA with primers to introduce a Shine-Delgarno sequence and a C-terminal 6x-His tag and cloned into the EcoRI and NotI sites of pGEX4T1-Rpb4 using T4 DNA ligase to generate pGEX4T1-Rpb4/7 (Addgene #138484).
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bioRxiv - Molecular Biology 2020Quote: ... Full-length UL87 was PCR amplified from HCMV Towne BAC DNA with primers to introduced EcoRI and XhoI sites and cloned into pcDNA4/TO-2xStrep (C-terminal tag) using T4 DNA ligase (Addgene #138434). Full-length mu24 was PCR amplified from MHV68-infected 3T3 cell cDNA with primers to introduce BamHI and NotI sites and cloned into pcDNA4/TO-2xStrep (C-terminal tag ...
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bioRxiv - Microbiology 2020Quote: ... A DNA fragment containing a codon-optimized version of hACE2 (Genbank NM_021804) was inserted into pLV-EF1a-IRES-Hygro (Addgene Plasmid #85134) using Gibson assembly ...
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bioRxiv - Microbiology 2021Quote: ... was synthesized by Integrated DNA Technologies (IDT, Coralville, IA) and cloned into the mEGFP-Lifeact-7 mammalian expression plasmid (Addgene #54610), replacing Lifeact and appending a 3x HA tag ...
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bioRxiv - Neuroscience 2022Quote: ... 90ul cells suspension containing 1M cells was mixed with 10 uL DNA mix: 4 ug pSpCas9(BB)-2A-Puro (PX459) plasmid (Addgene #48139), • 0.4 ug gRNA encoding plasmid (pKLV-U6gRNA(BbsI)-PGKzeo2ABFP ...
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bioRxiv - Immunology 2019Quote: We cloned template DNA for the indicated guide RNAs into a pLX-sgRNA construct additionally containing blasticidin resistance (Addgene plasmid #50662). We transfected constructs into HEK293T cells along with lentivirus packaging vector pSPAX2 (Addgene plasmid #12260 ...
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bioRxiv - Molecular Biology 2020Quote: ... Casper strains were available and 20 ng/μL DNA plasmids encoding mNG-GECO1 under the control of nuclear-localized elavl3/HuC promoter (Addgene: 59530) were injected into two-cell stage embryos of Casper mutant zebrafish33 with 40 ng/μl Tol2 transposase mRNA (26 ...
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bioRxiv - Genetics 2021Quote: sgRNAs were purchased as single-stranded DNA oligos (25nmoles standard desalted, IDT) with BbsI sticky ends and cloned in pairs into the pDG459 plasmid (Addgene #10090) for the deletion step using a one-step cloning reaction as described by the Thomas lab 25 The same protocol with the minor modification of replacing the second oligo with water during ligation was used to clone the syn-sgRNAs into pX459 (Addgene #62988) ...
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bioRxiv - Microbiology 2021Quote: ... The SPR tail tube gene was amplified from phage genomic DNA and cloned into the plasmid pBbS8k-RFP (Addgene, cat. #35276) with primers JG249 and JG250 for inducible expression in E ...
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bioRxiv - Microbiology 2021Quote: ... The gRNA library was synthesized as an oligonucleotide pool (Integrated DNA Technologies) and cloned into CROP-seq-opti (a gift from Jay Shendure, Addgene # 10628095) as described96 ...
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bioRxiv - Cancer Biology 2020Quote: ... 1.5 × 107 293T cells in 150Lmm tissue culture dishes were transfected with 18☐μg of each plasmid DNA along with 4.5 μg of pMD2.G (Addgene #12259) and 9 μg of psPAX2 packaging vectors (Addgene #12260 ...
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bioRxiv - Genomics 2022Quote: Viral stocks were produced by transfecting either 25 μg of pNL4.3 viral DNA or 45 μg HIV-GKO (Addgene Plasmid#112234) viral DNA together with 10 μg pMD.G packaging plasmid (45 ...
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bioRxiv - Microbiology 2022Quote: The full-length 3CLpro sequence of RHDV2 was codon-optimized for protein expression in mammalian cells, synthesized by Integrated DNA Technologies (Coralville, IA) and inserted into pcDNA3 H2B-mIFP T2A Mpro (3CLpro) (Addgene, MA) following digesting with BamH1 and ApaI ...
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bioRxiv - Cancer Biology 2022Quote: Wildtype full-length LRP8 was cloned from a MDA-MB-453 complementary DNA (cDNA) library and inserted into pLenti-CMV-GFP-Hygro (Addgene #17446) between XbaI and BamHI ...
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bioRxiv - Neuroscience 2020Quote: Plasmid Cry2olig-mCherry-tau 1-441 was prepared by inserting DNA fragment encoding the full length tau into the linearized Cry2olig-mCherry (Addgene 60032) backbone at the C-terminus of mCherry using Gibson assembly® Cloning kit (New England BioLab Int.) ...
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bioRxiv - Molecular Biology 2020Quote: ... Full-length mu24 was PCR amplified from MHV68-infected 3T3 cell cDNA with primers to introduce BamHI and NotI sites and cloned into pcDNA4/TO-2xStrep (C-terminal tag) using T4 DNA ligase (Addgene #138435). Full-length BcRF1 was PCR amplified from pcDNA4/TO-BcRF1-3xFLAG (19 ...
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bioRxiv - Neuroscience 2021Quote: ... HEK293T cells were transfected using Fugene HD transfection reagent in a 3:1 reagent : DNA ratio with packaging plasmid 2 µg psPAX2 (a gift from Didier Trono, Addgene, #12260), 1 µg murine ecotropic envelope plasmid pEnv(eco)-IRES-puro (Morita et al ...
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bioRxiv - Developmental Biology 2022Quote: ... we PCR amplified ~1kb homology arms from genomic DNA of the injection strain and cloned them into pHD-DsRed-attP (Addgene #51019). We co-injected guide RNAs and donor vectors (Rainbow Transgenics ...
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bioRxiv - Biophysics 2022Quote: ... The following expression vectors were used for plasmid DNA transfections: pCMV-mEmerald-FilaminA-N-9 (Addgene #54098, gift from Michael Davidson), pCMV-mCherry-FilaminA-N-9 (Addgene #55047 ...
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bioRxiv - Genetics 2019Quote: ... were ordered from Integrated DNA Technologies and after phosphorylation and annealing were cloned into pLKO.1 - TRC shRNA cloning vector (Addgene #10878) into AgeI/EcoRI site ...
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bioRxiv - Neuroscience 2019Quote: ... The donor plasmid for homologous recombination was constructed by using a Golden Gate assembly [92] to recombine four DNA elements: 1) A backbone with ampicillin resistance (pBS-GGAC-ATGC plasmid (a gift from Frank Schnorrer, Addgene #60949)[93] ...
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bioRxiv - Cancer Biology 2019Quote: To produce gRNA the following DNA oligos purchased from IDT were cloned into pSpCas9(BB)-2A-Puro (PX459) V2.0 vector (Addgene Plasmid #62988), using the protocol in 64) ...
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bioRxiv - Developmental Biology 2020Quote: ... in mouse G4 ES cells the HA-NLS-FlpO-WPRE-Sv40-pA-LoxP-PGK-Neo-polyA-LoxP cassette in frame with the ATG of the endogenous Apln gene (full plasmid sequence and DNA to be deposited at Addgene # reference). All primer sequences required to genotype this mice are provided in Extended Data Table 1.