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Citations for Addgene :
401 - 450 of 774 citations for Rat Proto oncogene Mas MAS1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The VSV-G encoding plasmid and lentiviral packaging plasmid psPAX2 were obtained from Addgene (Cambridge, MA). The pLenti-GFP lentiviral reporter plasmid that expresses GFP and luciferase was generously gifted by Fang Li ...
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bioRxiv - Cell Biology 2021Quote: ... and mEos3.2-LifeAct (no. 54696; a gift from Michael Davidson) were obtained from Addgene (Watertown, MA). 1 µg of plasmid was used to transfect 400,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5 µL ...
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bioRxiv - Cell Biology 2021Quote: ... and cloned into the BbsI site of pSpCas9(BB)-2A-GFP (pX458; Addgene, Cambridge, MA, USA). PX 458 was a gift from Feng Zhang (Addgene plasmid # 48138 ...
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bioRxiv - Bioengineering 2022Quote: ... Mammalian expression plasmids pRK5-EGFP-tau and pcDNA3-GSK3b were obtained from Addgene (Watertown, MA, USA). The pRK5-EGFP-tau (# 46904 ...
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bioRxiv - Genetics 2022Quote: ... which were annealed and cloned into the PUC57-gRNA expression vector (Addgene 51132, Cambridge, MA, USA) with a T7 promoter ...
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bioRxiv - Neuroscience 2023Quote: ... The pAAV-FLEX-tdTomato was a gift from Edward Boyden (Addgene plasmid #28306, Addgene, Watertown, MA).
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bioRxiv - Genetics 2023Quote: ... Muscle cells were then transfected with a pLenti-myc-GLUT4-mCherry (Addgene; Watertown, MA; stock # 64049) for 48 hours and the GLUT4 localization assay was performed as previously described(Lim ...
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bioRxiv - Neuroscience 2023Quote: ... AAVrg’s were purchased from Addgene and included either AAVrg-hSyn-Cre-p2A-dTomato (1.5x1013 vg/mL; 107738-AAVrg; Addgene, Watertown, MA), which was used to knockout PTEN ...
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bioRxiv - Neuroscience 2023Quote: ... The pAAV-FLEX-tdTomato was a gift from Edward Boyden (Addgene plasmid #28306, Addgene, Watertown, MA).
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bioRxiv - Cancer Biology 2023Quote: CRISPRoff-v2.1 was a gift from Luke Gilbert (Addgene plasmid # 167981;a Addgene, Watertown, MA, USA)9 ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified ULP1 protease was added to the eluent overnight at 4°C (pFGET19_Ulp1, Addgene, Watertown, MA). The cleaved product was loaded onto a Ni column (Cytvia HisTrap™ High Performance ...
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bioRxiv - Microbiology 2024Quote: ACE2+ 293 were transiently transfected with pALPS expressing tat-p2a-rev cassette (Addgene, Watertown, MA, USA). The TZM.bl cell were transiently transfected with Wuhan CoV-2 spike expression plasmid pHDM (BEI resources ...
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bioRxiv - Cell Biology 2024Quote: ... The lentiviral vectors were co-transfected with the packaging vectors pCMV-deltaR8 (Addgene, MA, USA, #12263) and pCMV-VsVg (Addgene ...
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bioRxiv - Physiology 2023Quote: ... the pAS_4xG1 PylT FLAG-G1 PylRS Y125A tRNA/aminoacyl-tRNA synthetase plasmid (Addgene, Watertown, MA #154773) was used ...
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bioRxiv - Neuroscience 2023Quote: ... viral prep #50477-AAV8) or eGFP (AAV8-CaMKIIa-EGFP, Addgene, Cambridge, MA, viral prep #50469-AAV8). Craniotomies were created ...
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bioRxiv - Genomics 2022Quote: ... elegans Vector Kit was a gift from Andrew Fire (Addgene kit # 1000000001). A translational reporter was later generated by synthesizing the remainder of the coding sequence (IDT ...
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bioRxiv - Cancer Biology 2021Quote: Galectin-3 cDNA was subcloned into the pMIG-GFP retroviral expression vector (plasmid #9044, AddGene, Cambridge, MA). Stably transduced hGalectin-3 expressing OP9 cells were generated by infecting cells with retroviral particles and FACS sorting of GFP expressing (positively transduced ...
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bioRxiv - Cancer Biology 2021Quote: ... H4 human GBM cells were infected with the whole-genome knockout Brunello library (Addgene, Cambridge, MA, USA), which included ∼19,000 genes with 4 sgRNAs per gene and 10,000 sgRNA non-targeting controls ...
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bioRxiv - Neuroscience 2021Quote: ... the DNA plasmids were respectively derived from the plasmids pAAV:EF1α:DIO:ChETA-eYFP (plasmid #26968; Addgene, Watertown, MA, USA) and pAAV:EF1α:DIO:eYFP (Addgene plasmid #27056 ...
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bioRxiv - Cancer Biology 2019Quote: ... Double strands of miR21a-gRNA were synthesised and cloned to LentiCRISPR-V2 plasmid (Addgene, Cambridge, MA, USA) by TsingKe Ltd ...
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bioRxiv - Cancer Biology 2020Quote: ... Human codon-optimized Streptococcus pyogenes wild type Cas9 (Cas9-2A-GFP) was obtained from Addgene (Cambridge, MA). Chimeric guide RNA expression cassettes with different sgRNAs (sgRNA1 ...
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bioRxiv - Genetics 2019Quote: ... The wildtype human DYRK1A cDNA was cut from the pMH-SFB-DYRK1A construct (Addgene, Cambridge, MA, USA) and inserted into pCS2-HA vector using XhoI and contains a gateway vector site 5’ to the cDNA ...
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bioRxiv - Biophysics 2020Quote: ... psPAX2 packaging plasmid (plasmid #12260) and pMD2.G envelope plasmid (plasmid #12259) were from Addgene (Watertown, MA). N-terminally Cy5-labeled peptides were synthesized and purified to > 95% purity by Gen-Script (Piscataway ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The WT human α4 and β1 subunits were subcloned into the pUNIV vector (Addgene, Cambridge, MA, USA) and the human δ-subunit into the pcDNA5/FRT vector (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: The ORAI1-yellow fluorescent protein (YFP) construct was purchased from Addgene (Cambridge, MA, USA; plasmid no. 19756). Site directed mutagenesis using the Pfu Turbo DNA polymerase from Agilent Technologies (Santa Clara ...
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bioRxiv - Cell Biology 2020Quote: ... Whole-genome lentiviral pooled CRISPR/Cas9 knockout library (Cat# 73178-LV) was obtained from Addgene (Cambridge, MA). Alexa FluorTM 568 carboxylic acid succinyl ester ...
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bioRxiv - Biochemistry 2022Quote: ... cerevisiae eIF6A was purified essentially as described before (45) from the p7XC3GH (Addgene #47066; Watertown, MA, USA) plasmid ...
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bioRxiv - Bioengineering 2020Quote: ... lentivirus encoding GFP was produced using plasmid for GFP expression (CMV-GFP) purchased from Addgene (Cambridge MA). Transduction was carried out using CMV-GFP lentiviruses in growth media with 8 μg/ml polybrene (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... annealed and cloned at BbsI enzyme restriction site into the px330 modified vector (Addgene, Watertown, MA, USA) (Etoc et al. ...
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bioRxiv - Neuroscience 2020Quote: ... the virus used was AAV8-CaMKIIα-hM3D(Gq)-mCherry (Addgene viral prep # 50476-AAV8, Addgene, Cambridge, MA). In the GFP (null virus ...
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bioRxiv - Neuroscience 2020Quote: ... the virus used was AAV8-CaMKIIα-hM4D(Gi)-mCherry (Addgene viral prep # 50477-AAV8, Addgene, Cambridge, MA). In the Gq experiment ...
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bioRxiv - Neuroscience 2020Quote: ... AAV1-hSyn1-SIO-stGtACR1-FusionRed and AAV2-hSyn-DIO-mCherry were produced by Addgene (Watertown, MA, USA). All viral vectors were stored in aliquots at −80°C until use.
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bioRxiv - Cancer Biology 2021Quote: ... H4 human GBM cells were infected with the whole-genome knockout Brunello library (Addgene, Cambridge, MA, USA), which covered ~19,000 genes with 4 sgRNAs per gene along with 10,000 sgRNA non-targeting controls ...
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bioRxiv - Immunology 2021Quote: ... Sleeping Beauty (SB) transposon plasmid pSBbi-BH23 (a gift from Eric Kowarz; Addgene # 60515, Cambridge, MA, USA) was digested at SfiI sites (NEB ...
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bioRxiv - Genomics 2021Quote: ... Early passage primary MEFs were transformed with SV-40 T antigen containing plasmid pBSSVD2005 (ADDGENE, Cambridge, MA) to generate immortalized MEFs ...
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bioRxiv - Molecular Biology 2022Quote: ... then a fragment of CRY2olig region that was digested from pCRY2olig-mCherry (#60032; Addgene, Watertown, MA, USA) was inserted at the NheI and SmaI sites (pCRY-C220- ...
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bioRxiv - Molecular Biology 2022Quote: Templates for in vitro transcription of SpCas9 guides were amplified from the plasmid pX330 (Addgene, Watertown, MA) using primer pairs with 65 oligonucleotides as a 5’ primer (forward ...
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bioRxiv - Molecular Biology 2022Quote: ... A puromycin resistant gene unit was amplified from plasmid pGL3-U6-sgRNA-PGK-puromycin (Addgene, Watertown, MA) by PCR and the 1473 bp product was then inserted in an EcoRV site in intron 19-20 ...
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bioRxiv - Developmental Biology 2022Quote: Full length inpp5ka (NM_001089493.1) and inpp5kb (XM_021479345.1) cDNAs were cloned into the pCS2+ plasmid (Addgene, Watertown, MA). Digoxygenin-labeled sense and antisense probes were synthesized from the linearized plasmids using the DIG RNA Labeling Kit (SP6/T7 ...
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bioRxiv - Neuroscience 2023Quote: ... 400 nl of AAV2/5-CAG-dLight1.1 (1.7×1013 GC/ml, 111067-AAV5, Addgene, Watertown, MA, USA) was slowly injected into the DMS (n = 7 ...
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bioRxiv - Neuroscience 2023Quote: ... or rAAV5-hsyn-DIO-mCherry (around 5.0 x 1012 vg/ml for each) (Addgene, Watertown, MA, USA) in the dorsal raphe nucleus [Anterior-posterior (AP) ...
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bioRxiv - Molecular Biology 2023Quote: The lentiviral shuttle vector coding for mCherry (LV-mCherry) was a from Addgene (#36084, Cambridge, MA, USA). LV-TK ...
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bioRxiv - Microbiology 2023Quote: ... the DsRed gene was PCR amplifed from the pMSCV-IRES-DsRed Fp vector (Addgene, Watertown, MA, USA) and cloned via HindIII and KpnI into the pBlueScript KS+ vector to generate pBS_DsRed ...
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bioRxiv - Cancer Biology 2023Quote: ... A CRISPR/dCas9 vector was constructed as follows: pX330A_dCas9-1×2 (Addgene, Watertown, MA; plasmid ID 63596) (20 ...
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bioRxiv - Neuroscience 2023Quote: ... viral prep #50477-AAV8) or eGFP (AAV8-CaMKIIa-EGFP, Addgene, Cambridge, MA, Addgene, viral prep #50469-AAV8) infusion and unilateral chronic implantation of electrode arrays in both ACC and OFC ...
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bioRxiv - Biophysics 2023Quote: ... pCAGGS-SARS-CoV-2 S D614G (# 156421) and pcDNA3.1 vectors were obtained from Addgene (Watertown, MA, USA), and Invitrogen (Waltham ...
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bioRxiv - Cancer Biology 2023Quote: ... sgRNAs targeting either MGA or NME1 was cloned in pLKO5.sgRNA.EFS.mCherry or pLKO5.sgRNA.EFS.GFP (Addgene, Watertown, MA), respectively ...
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bioRxiv - Immunology 2023Quote: ... sgRNA targeting Myc was cloned into a Cas9-expressing lentiviral transfer vector (lentiCRISPRv2, # 52961, Addgene, Cambridge, MA) following the methods of the Feng Zhang laboratory (Sanjana et al. ...
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bioRxiv - Neuroscience 2023Quote: 1-4 evenly spaced ∼60 nl injections of the AAV1-synapsin-l-GCamp6f (Addgene, MA stock #100837) that had been diluted to a titer of ∼1×10^12 vg/mL using sterile PBS were made in each cranial window ...
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bioRxiv - Neuroscience 2023Quote: ... we injected AAV9-CaMKIIa.C1V1(t/t).TS.EYFP (titer: 2.3 × 1013 GC/ml; prepared by Addgene, MA, USA) into the unilateral dorsal hippocampus as described previously 87.