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Citations for Addgene :
501 - 550 of 774 citations for Rat Proto oncogene Mas MAS1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... 200nl of adeno-associated virus 2 (AAV2) containing either control construct (pAAV-hSyn-EGFP; plasmid #50465; Addgene, Watertown, MA) or excitatory DREADD (pAAV-hSyn-hM3D(Gq)-mCherry ...
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bioRxiv - Neuroscience 2020Quote: ... to measure BLA ACh levels (Fig. 2A-E + S2.1-S2.2) or 0.5 µL of AAV1 Syn-FLEX-GCaMP6s-WPRE-SV40 (Addgene, Watertown, MA) to measure BLA principal cell calcium dynamics (Fig ...
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bioRxiv - Microbiology 2020Quote: ... The CD4 expression vector pMX-CD4 and the TR expression vector pMD18-T TR were from Addgene (Watertown, MA) and Sino Biological Inc ...
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bioRxiv - Biochemistry 2020Quote: The MSP1E3D1 plasmid (with a His-tag as well as a TEV protease cleavage site, from Addgene, MA, USA) (26 ...
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bioRxiv - Neuroscience 2021Quote: ... Adeno-associated virus containing the GCaMP7f gene (pGP-AAV9-syn-FLEX-jGCaMP7f-WPRE, 104488-AAV9, Addgene, Watertown, MA, USA) was loaded into a glass micropipette with a tip diameter of 40–50 µm attached to a Nanoject II injection system (Drummond Scientific ...
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bioRxiv - Cell Biology 2021Quote: The ATeam1.03-nD/nA/pcDNA3 plasmid was a gift from Takeharu Nagai (Addgene, Watertown, MA, plasmid # 51958; http://n2t.net/addgene:51958; RRID: Addgene_51958) (Kotera et al. ...
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bioRxiv - Neuroscience 2021Quote: ... AAVrg-CAG-GFP (titer ≥ 7×1012vg/mL, category number 37825, lot V9234) was purchased from Addgene (Watertown, MA, USA).
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bioRxiv - Cancer Biology 2022Quote: ... This middle entry vector was Gateway recombined using a 5’ entry zebrafish ubiquitious promoter (Addgene #2732, Watertown, MA, USA), a 3’ entry pA vector ...
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bioRxiv - Plant Biology 2022Quote: ... and HlWRKY1 was individually cloned into the bait vector pGBKT7-GW [DNA-binding domain (BD)] (Addgene Inc, MA, USA) to produce fusion proteins containing a GAL4 activation domain (AD ...
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bioRxiv - Neuroscience 2023Quote: ... and pAAV2-hSyn-DIO-EGFP retrograde virus construct (7.6×1012 genome copies/ml; 50457-AAVrg, Addgene, Watertown, MA, USA) was made with a flow rate of 1.0 µl/min ...
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bioRxiv - Molecular Biology 2022Quote: The lentiCRISPR v2 (#52961)43 and lentiCRISPR v2-dCas9 (#112233)44 vectors were purchased from Addgene (Watertown, MA, US).
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bioRxiv - Neuroscience 2023Quote: ... with CRMP1 then being subcloned into pENTR1A no ccDB (20) (Dr. Eric Campeau, AddGene clone 17398, Watertown, MA, USA). Vectors encoding NPAS3 (13 ...
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bioRxiv - Physiology 2023Quote: ... for generation of isogenic control lines were designed using an online tool (crispr.mit.edu) and ordered as oligos to be cloned into pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene, MA, USA).
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bioRxiv - Cancer Biology 2022Quote: ... Ptch;p53 primary cell-line(#4954) was transduced using viral particles containing the lentiCas9-Blast vector (Addgene, Watertown, MA; RRID:Addgene_52962). Cells stably expressing Cas9 were selected using 10ug/ml Blasticidin S HCl (Gibco™-#R21001) ...
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bioRxiv - Microbiology 2023Quote: N4BP1 KO Huh7 cells were generated by CRISPR/Cas9 using pX330-U6-Chimeric_BB-CBh-hSpCas9 (pX330, Addgene, MA, USA) and HR110PA-1 (System Biosciences ...
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bioRxiv - Plant Biology 2024Quote: ... S1) targeting the OsNAC5 gene was designed and cloned into the pRGEB31 CRISPR/Cas9 vector (Addgene, Watertown, MA, USA) (Xie and Yang ...
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bioRxiv - Genetics 2024Quote: Human pcDNA3-FLAG-RBBP5 plasmid used in the protein expression experiments was obtained from Addgene (Cat# 15550, MA, USA). Candidate variants were introduced using QuikChange II Site-directed mutagenesis kit according to manufacturer’s protocol (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... on p26 subjects underwent a stereotaxic surgical injection of the inhibitory DREADD AAV-CAMKIIa-HM4DGi-mCherry (Addgene, Watertown, MA) to the BLA (A/P ...
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bioRxiv - Neuroscience 2024Quote: ... A genetically encoded calcium indicator for the detection of ACh activity (1 μL, AAV9-hSyn-ACh3.0; AddGene; Watertown, MA) was infused into the medial prefrontal cortex (AP(2.7mm) ...
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bioRxiv - Cell Biology 2024Quote: ... shRNAs targeting luciferase or mouse Nr1h3 and Rara were cloned into the pLKO.1 vector (Addgene, MA, USA, #8453). The lentiviral vectors were co-transfected with the packaging vectors pCMV-deltaR8 (Addgene ...
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bioRxiv - Bioengineering 2021Quote: ... The GoldenPiCS Kit was a gift from the Gasser/Mattanovich/Sauer group (Addgene kit #1000000133). All coding sequences were amplified with high-fidelity Phusion DNA Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... Basic DNA parts were selected from GoldenBraid 2.0 kit from Diego Orzaez (Addgene kit # 1000000076) or MoClo Toolkit ...
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bioRxiv - Neuroscience 2020Quote: The TALEN kit used for TALE assembly was a gift from Keith Joung (Addgene kit # 1000000017). DNA fragments encoding ROSA TALEN repeat arrays were cloned into plasmid pJDS71 ...
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bioRxiv - Plant Biology 2024Quote: We used zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9 (AddGene #1000000171). Sequence of all oligonucleotides and PCR primers used in this study are provided in Table S1 ...
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bioRxiv - Molecular Biology 2020Quote: ... and miR 128a were inserted into the 3’UTR of pMIR luciferase reporter (Life Scientific). miR-10b reporters described previously (Ma et al. 2007) were obtained from AddGene. Reporters were co-transfected with Renilla luciferase (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... full-length Npac/Glyr1 cDNA was ligated into MluI and NotI restriction sites of pMXs plasmid (18656, Addgene. Watertown, MA). To construct Npac mutant plasmid that produces functional Npac protein but was resistant to Npac RNAi targeting ...
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bioRxiv - Neuroscience 2019Quote: ... CA) or the excitatory DREADD receptor-encoding virus (AAV5-hSyn-DIO-hM3Dq-mCherry, 6.50E+12 GC/mL, Addgene, Watertown, MA). Viral incubation occurred for at least 8 weeks during the post-surgical recovery ...
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bioRxiv - Neuroscience 2020Quote: βII-spectrin-ΔCH-GFP and βII-spectrin-ΔPH-GFP plasmids were modified from FUGW-GFP plasmid (Addgene, 14883, Cambridge, MA). In details ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: HEK293 cells were transfected in 96-well plates with the 8xGTIIC-luciferase plasmid (firefly luciferase, # 34615, Addgene, Watertown, MA, US) and the pRL-SVl40P plasmid (Renilla luciferase ...
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral particles were produced in HEK293T cells by transfection with 0.2 µg of pCMV-VSV-G (Addgene, #8454, MA, USA), 2 µg of psPAX2 (Addgene ...
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bioRxiv - Cell Biology 2022Quote: The Spike gene was cleaved from pcDNA-Spike plasmid and cloned into lentiviral vector pLV-mCherry (Addgene, Watertown, MA, USA) with removal of mCherry gene to generate pLV-Spike plasmid ...
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bioRxiv - Physiology 2020Quote: ... LRMP- and IRAG-specific gRNA sequences were cloned into the pSpCas9(BB)-2A-GFP vector (Addgene, Watertown, MA; Cat. #PX458). Two distinct cut sites were used for each IRAG (XM_027410089.1 ...
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bioRxiv - Immunology 2021Quote: ... par-5 and his-1 cDNA were subcloned into pCE-BiFC-VN173 and pCE-BiFC-VC155 plasmids (Addgene, Cambridge, MA), which contain the heat shock promoter Phsp-16.41 ...
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bioRxiv - Genetics 2019Quote: ... The empty guide RNA (gRNA) expression vector (ID 41824) and hCas9 vector (ID 41815) were obtained from Addgene (Cambridge, MA). The gRNA target sequences GAAGAGGTGAACTGCCTTT (NIPBL exon 3 ...
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bioRxiv - Genomics 2021Quote: ... donor DNA constructs were chemically synthesized and cloned into the pUC19 or pAAV-MCS2 plasmids obtained from Addgene (Watertown, MA). The donors included 400-800 bp homology arms flanking the inserted DNA sequence encoding the FKBP12 degradation tag as well as mScarlet ...
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bioRxiv - Cancer Biology 2021Quote: ... Sequences were modified according to the Zhang Lab Target Guide Sequence Cloning Protocol using the lentiCRISPRv2 plasmid (Addgene, Watertown, MA) (34-36) ...
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bioRxiv - Cell Biology 2021Quote: ... All the plasmids for Genome-wide CRISPR library generation (GeCKOv2) and individual gene knockouts were procured from Addgene (MA, USA). CellLight Tubulin-RFP / GFP ...
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bioRxiv - Neuroscience 2021Quote: ... AAV2/hSyn-DIO-hM3Dq-mCherry (#44361) and AAV2/hSyn-DIO-hM4Di-mCherry (# 44362) were obtained from Addgene (Watertown, MA, USA). AA2/SST-Cre (#CV17210-AV2 ...
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bioRxiv - Cell Biology 2022Quote: ... GFP::RPT-5 translational fusion construct was created by substituting the let-858 promoter of the GFP vector pPD118.25 (L3786 was a gift from Andrew Fire; Addgene plasmid #1593; http://n2t.net/addgene:1593; RRID:Addgene_1593; Watertown, MA, USA) with a 2010-bp putative rpt-5 promoter sequence ...
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bioRxiv - Molecular Biology 2022Quote: ... we cloned CstF64 coding sequence into pLIX_402 vector backbone (a gift from David Root, Broad Institute, MA) and pLIX_402 vector (Addgene plasmid #41394) was used as control ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... A human elongation factor 1 (EF1) promoter was introduced using a Gateway att4-att1 Entry clone (Addgene, Watertown, MA, #162920). Final expression clones were verified by restriction analysis and maxiprep DNA was prepared using the Qiaprep Maxiprep kit (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... and the selected target sequences were inserted in the pSpCas9(BB)-2A-GFP plasmid, which was a gift from Feng Zhang (Massachusetts Institute of Technology, Cambridge, MA, USA) (Addgene plasmid # 48138 ...
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bioRxiv - Biophysics 2023Quote: ... Plasmid DNA for expressing CRY2olig tagged with mCherry at its C-terminus was obtained from Addgene (#60032; Watertown, MA, USA). The plasmid (1.0 µg/dish ...
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bioRxiv - Developmental Biology 2022Quote: ... Complementary oligonucleotides containing the miR-23a or miR-27a sgRNA target sequences were designed by CRISPR Design Tool (http://crispr.mit.edu) and inserted into the pX335 plasmid (Addgene, Cambridge, MA), followed by DNA sequencing to verify the correct cloning ...
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bioRxiv - Cancer Biology 2022Quote: ... Ptch;p53 primary cell-line(#4954) was transduced using viral particles containing the lentiCas9-Blast vector (Addgene, Watertown, MA; RRID:Addgene_52962). Cells stably expressing Cas9 were selected using 10ug/ml Blasticidin S HCl (Gibco™-#R21001) ...
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bioRxiv - Cancer Biology 2023Quote: ... The packaging plasmids pCMV-VSV-g (cat# 8454) and pS-PAX2 (cat# 12260) were obtained from Addgene (Watertown, MA, USA).
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bioRxiv - Cell Biology 2023Quote: ... Oligonucleotides for cloning guide RNA into pSpCas9 (BB)-2A-GFP vector (48138; a gift from F. Zhang, Addgene, Cambridge, MA) were designed as described previously 63 ...
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bioRxiv - Cancer Biology 2023Quote: ... A 250 µL aliquot of lentiviral cocktail containing either Lenti-Cas9-Blast plasmid (SU-DIPG-XIII; Addgene, Watertown, MA, USA) or Lenti-Cas9-2A-Blast (SU-DIPG-XXXVI ...
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bioRxiv - Neuroscience 2024Quote: ... Either AAV-CAG-FLEXFRT-ChR2(H134R)-mCherry (n = 7, 200 nL, 3 × 1012 GC/mL, Serotype: 9; Addgene, MA, USA) to express channelrhodopsin (ChR2 ...
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bioRxiv - Neuroscience 2024Quote: ... The APP gRNA sequence was cloned into pSpCas9 (BB)-2A-Puro (PX495) v.2.0 (plasmid #62988; Addgene, Cambridge, MA, USA) using the method described by Ran et al.48 ...