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Citations for Addgene :
351 - 400 of 1062 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... a 3XFLAG-NatMX cassette was PCR-amplified from pYC46 plasmid (Addgene) and inserted at the C-terminus of ZCF27 and ZCF4 (74 ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR fragments were assembled into pLenti6.3/V5- DEST-GFP (Addgene #40125) by replacing the CMV promoter and EGFP sequences at the 5’ ClaI and 3’ SalI restriction sites ...
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bioRxiv - Plant Biology 2024Quote: ... The PCR amplicons were cloned into the pAGM9121 (Addgene plasmid #51833), pICH41308 (Addgene plasmid #47998 ...
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bioRxiv - Cell Biology 2024Quote: ... The PCR product was further cloned into pGEX-4T-1 (Addgene) in-frame with the N-terminal GST-tagged fusion construct(21) ...
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bioRxiv - Cell Biology 2024Quote: ... the CRY2 PHR domain was PCR amplified from pCRY2PHR-mCherryN1 (Addgene plasmid #26866 ...
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bioRxiv - Cancer Biology 2021Quote: ... The PCR product was cloned into the pENTR4 no ccDB plasmid (Addgene #17424 ...
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bioRxiv - Cell Biology 2020Quote: ... full length P180 was PCR amplified from V5-GFP-P180 (Addgene #92150) and inserted in mCherry-N1 vector between XhoI and BamHI sites ...
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bioRxiv - Cell Biology 2019Quote: ... PCR products and a pCS2+8 vector (Gokirmak et al., 2012) (Addgene) were digested with XbaI and BamHI (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... the 24xMS2 stem loop sequence was purified from pCR-24xMS2L-stable (Addgene_31865) by restriction digest and the 128xMS2 from pMK123-128xMS2 (Tantale et al. ...
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bioRxiv - Cell Biology 2019Quote: Human STAMBPL1 was PCR amplified from FLAG-HA-STAMBPL1 (Addgene plasmid #22559), human TBC1 domain containing kinase (TBCK ...
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bioRxiv - Genetics 2020Quote: The tdTomato coding sequence was PCR-amplified from pDEST-HemmarR2 (Addgene # 112813) and cloned into EcoRI/XbaI sites of pUA (Addgene 58372).
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bioRxiv - Genetics 2020Quote: ... Cas9 coding sequence was PCR-amplified using pEDST-APIC-Cas9 (Addgene # 121657) as the template ...
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bioRxiv - Cell Biology 2019Quote: The fluorescent Gal3 reporter was PCR amplified from pEGFP-hGal3 (Addgene #73080), a gift from Tamotsu Yoshimori ...
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bioRxiv - Bioengineering 2021Quote: ... fragments were PCR-amplified and cloned into the px552 plasmid (Addgene #60958) to make px552-U6-CAG-mCherry ...
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bioRxiv - Immunology 2021Quote: ... BRD9-dBD and BRD9-N216A were PCR-amplified from existing vectors (Addgene plasmids #6537968 and #75114-648 ...
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bioRxiv - Cell Biology 2021Quote: ... The resulting PCR product was inserted into pLVpuro-CMV-N-EGFP (Addgene plasmid # 122848 ...
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bioRxiv - Cell Biology 2021Quote: ... PCR fragments of CgB (DNASU) and CLIP (gift from Eric Campeau; RRID:Addgene_29650) were subcloned into pENTR-RIP via Gibson assembly ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR-amplified UGI DNA from pLenti-TRE3G-FNLS-PGK-Puro (Addgene# 110847) and an NheI/PmeI-digested piggyBac dox inducible expression vector PB-TRE-Cas91 including a puromycin selection marker ...
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bioRxiv - Synthetic Biology 2022Quote: ... pCasTn plasmid cloning: ShCAST genes were PCR amplified from pHelper_ShCAST_sgRNA (Addgene #127921). The vector backbones were amplified from plasmids carrying an RK2 origin of replication with a Kanamycin resistance gene for pKSh2 (Addgene #167515 ...
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bioRxiv - Neuroscience 2020Quote: ... dLight1.1 sequence was PCR amplified from pCMV.dLight1.1 (Addgene #111053, Dr. Lin Tian)56 using Q5 polymerase (primers ...
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bioRxiv - Biochemistry 2022Quote: ... mCherry was PCR amplified from mCherry-Alpha-5-Integrin-12 (Addgene #54970) using forward primer 54970RMmCherryaddNHis_F2 ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR with pKD3 (pKD3 was a gift from Barry L. Wanner; Addgene plasmid #45604 ...
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bioRxiv - Genetics 2020Quote: ... The His2Av polyA sequence was PCR amplified from pDEST-APLO (Addgene 112805). DNA fragments were assembled together using NEBuilder DNA Assembly (New England Biolabs Inc.).
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bioRxiv - Neuroscience 2020Quote: ... secA5 sequences were amplified by PCR from pBH-UAS-secA5-YFP (Addgene) and mCherry sequences were amplified by PCR from pAAV-hSyn-mCherry (Addgene ...
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bioRxiv - Neuroscience 2020Quote: ... and mCherry sequences were amplified by PCR from pAAV-hSyn-mCherry (Addgene) sequences ...
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bioRxiv - Biochemistry 2020Quote: ... The EMCV IRES sequence was amplified by PCR from EMCV_IRES_pcDNA4TO_H2B_SunTag24x_v1 (Addgene #246719) using the following primers ...
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bioRxiv - Genomics 2021Quote: ... The PCR amplicon was cloned into a lacZ reporter vector (Addgene #139098) using Gibson assembly (New England Biolabs)57 ...
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bioRxiv - Cancer Biology 2020Quote: ... The MetRSL274G coding sequence was PCR amplified using pMarsL274G construct (Addgene 63177) as a template and inserted into BamHI and MluI sites within the pLV-EF1a-IRES-puro vector (Addgene 85132 ...
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bioRxiv - Cancer Biology 2021Quote: ... TUBA1B was amplified using PCR (donor plasmid: Addgene #57159, from Michael Davidson) and an EGFP-TUBA1B fusion protein was generated with two-stage overhang extension PCR using the TUBA1B and EGFP cDNA fragments ...
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bioRxiv - Molecular Biology 2020Quote: ... The 6xHis-MBP tag was PCR amplified from pDZ2087 construct (Addgene, #92414) with primers MBP F and MBP R ...
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bioRxiv - Cell Biology 2022Quote: ... 24xSuntag (24xGCN4) was PCR-amplified from pcDNA4TO-24xGCN4_v4-kif18b-24xPP7 (Addgene, #74928) and inserted between the BsrGI and ApaI sites of pcDNA-MCP-mCherry.
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bioRxiv - Molecular Biology 2022Quote: ... IκBα DNA was PCR amplified from pBABE-GFP-IκBα-wt (Addgene, 15263) and was digested and cloned into pET28-CBP-His6 ...
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bioRxiv - Neuroscience 2022Quote: ... we first PCR amplified myr- 4xSNAPf from pUAS-myr-4xSNAPf (Addgene #87637) using FRT-containing primers ...
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bioRxiv - Genomics 2022Quote: ... KRAB(ZIM3) was PCR-amplified from pLX303-ZIM3-KRAB-dCas9 (Addgene # 154472). The final construct is KRAB(ZIM3)-dCas9 or Zim3-dCas9 for short ...
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bioRxiv - Neuroscience 2022Quote: ... the YTHDF2 ORF was PCR amplified from pcDNA-flag-YTHDF2 (Addgene, #52300). The resulting amplicon was digested with AgeI and XbaI and cloned into cut sites upstream of HaloTag insert in pGW1-Halo.
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bioRxiv - Neuroscience 2022Quote: ... the YTHDF2 ORF was PCR amplified from pcDNA-flag-YTHDF2 (Addgene, #52300). The resulting amplicon was digested with KpnI and SalI and cloned into cut sites upstream of the 2A in the pGW1-2A-GFP vector.
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bioRxiv - Systems Biology 2024Quote: ... CYC1t and pTEF1 were both PCR amplified from BB3cK_pGAP_23*_pTEF_Cas9 (Addgene #104909) (Gassler et al. ...
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bioRxiv - Cell Biology 2023Quote: ... with HA-TurboID amplified by PCR from 3xHA-TurboID-NLS_pCDNA3 (Addgene #107171) and inserted with a Gly-Gly-Gly-Ser linker either side of the HA-TurboID sequence ...
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bioRxiv - Developmental Biology 2023Quote: ... the PCR amplicon was cloned into a lacZ-reporter vector (Addgene #139098) or mCherry reporter vector (available upon request ...
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bioRxiv - Microbiology 2023Quote: ... mKO2 was PCR amplified from the template mKO2-N1 (Addgene, Cat. #4625) using primers containing matching restriction sites ...
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bioRxiv - Pathology 2023Quote: Cre recombinase DNA was amplified by PCR using pCAG-Cre (Addgene #13775) as a template ...
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bioRxiv - Cancer Biology 2023Quote: ... The IRES site was amplified by PCR from pInducer-21 (Addgene #46948). YAP5SA was amplified by PCR from Addgene #33093 ...
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bioRxiv - Bioengineering 2023Quote: ... CjCas9 was PCR-amplified from pAAV-EFS-CjCas9-eGFP-HIF1a (Addgene #137929) as two overlapping fragments using primers to create D8A and H559A mutations ...
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bioRxiv - Cancer Biology 2023Quote: ... The bGH poly(A) signal was PCR amplified from pX459 (Addgene, 62988) using primers forward ...
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bioRxiv - Immunology 2023Quote: ... eGFP was amplified via PCR from pLJM-1-eGFP (Addgene: Plasmid #19319) using primers that overlapped with pLenti-EF1-Blast at the xho1 site on the 5’ end and overlapped with the 5’-end of the RNase L coding sequence on the 3’-end (Supplementary table 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... were ordered from GenScript (https://www.genscript.com) and PCR-cloned into pHIV-Luc-ZsGreen (Addgene plasmid #39196) using and EcoRI and XbaI digestion.
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bioRxiv - Neuroscience 2023Quote: ... and a synthetic intron was PCR-amplified from pDEST-APLO (Addgene 112805) (77) ...
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bioRxiv - Cell Biology 2023Quote: ... by PCR amplifying the ORFs from cDNA templates of HSC70 (19514, Addgene) and LAMP2A (86146 ...
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bioRxiv - Neuroscience 2023Quote: ... a Cas9-T2A fragment PCR amplified from pDEST-APIC-Cas9 (Addgene 121657) (9) ...
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bioRxiv - Cell Biology 2024Quote: ... GTS-mTagBFP and mTFP1-Actin were respectively amplified by PCR from Addgene 206256 ...