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Citations for Addgene :
301 - 350 of 829 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... DNA fragment of mito-EmGFP and PGK promoter (a gift from Tyler Jacks (Addgene plasmid # 11586 ...
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bioRxiv - Developmental Biology 2022Quote: DNA constructs reported in this study are available from Addgene (plasmid ID: 176836-176841). Genetically modified cells used in this study will be available from the corresponding author with an MTA ...
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bioRxiv - Genomics 2020Quote: ... sgRNA cassettes were synthesized (Integrated DNA Technologies) and cloned into lentiGuide-Puro (Addgene, 52963) vector ...
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bioRxiv - Neuroscience 2019Quote: ... plasmid DNA mixed with either pCAG-ChR2-Venus (Petreanu et al., 2007) (Addgene #15753) or pCAG-ChETA-EYFP (1.5μg/μL) ...
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bioRxiv - Cell Biology 2019Quote: The following DNA constructs were used in this study: EGFP-PFN1 (Plasmid #56438, Addgene), Lifeact mRuby (pN1-Lifeact-mRuby ...
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bioRxiv - Biophysics 2021Quote: ... with 1 μg of DNA plasmid vector Cry2Olig-mCherry (purchased from Addgene, number 60032), and then platted on fluorodishes ...
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bioRxiv - Cell Biology 2021Quote: ... Oligo DNAs for the sgRNA were cloned into the lentiCRISPRv2 (Addgene Plasmid: no. 52961) vector or pX459 (Addgene Plasmid ...
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bioRxiv - Synthetic Biology 2021Quote: ... and electroporated with the linear library DNA as well as the SS9_RNA (Addgene #71656) vector containing the guide RNA for Cas9 to target the safe harbor ...
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bioRxiv - Neuroscience 2021Quote: ... 1 million cells were nucleofected with iμg pAAV-CAG-GFP plasmid DNA (Addgene 37825) in Ingenio electroporation buffer (Mirus MIR50111 ...
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bioRxiv - Cell Biology 2019Quote: ... Annealed double strand DNAs were ligated into pSpCas9(BB)-2A-GFP (PX458) vector (Addgene) at the Bpi1 (Bbs1 ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: ... The synthesized oligo DNA primers for the guides were inserted into pX330 (Addgene #42230). For Tet-inducible expression of exogenous TopoII ...
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bioRxiv - Neuroscience 2022Quote: ... The TauRD(P301S)-YFP plasmid DNA with virus packing pDNAs (psPAX2; pMD2.G, Addgene) was transfected into 293T cells by 1mg/mL PEI transfection reagent and incubated for 36 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were transfected with 0.24μg EGFP-LC3 plasmid DNA (Addgene #11546; Watertown, MA, USA) using the FUGENE6 transfection reagent (Promega ...
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bioRxiv - Genomics 2023Quote: ... 2.5 ug of DNA (the pegRNA plasmid and the pCMV-PE2 plasmid (Addgene #132775) at a 1:2 ratio by mass of pegRNA plasmid:PE2) ...
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bioRxiv - Neuroscience 2023Quote: ... either rescue construct along with two helper DNA constructs (pHR-CMV8.2 deltaR (Addgene 8454) and pCMV-VSVG (Addgene 8455) ...
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bioRxiv - Cell Biology 2023Quote: ... DNA templates for PCR products were as follows - myo-3p from pCFJ104 (Addgene #19328), pgl-1::GFP::FLAG from DUP75 (Andralojc et al. ...
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bioRxiv - Genetics 2023Quote: ... DNA plasmids used for transgene cloning were lenti-EF1α-dCas9-VPR-Puro (Addgene 99373) (24 ...
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bioRxiv - Cell Biology 2023Quote: ... Oligo DNAs for the sgRNA were cloned into the lentiCRISPRv2 (plasmid no. 52961; Addgene) vector or pX459 (plasmid no ...
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bioRxiv - Neuroscience 2024Quote: ... pCAG mt-SypHer was created by PCRing the DNA encoding mt-SypHer from Addgene plasmid #48251 (a gift from Nicolas Demaurex) ...
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bioRxiv - Neuroscience 2024Quote: ... pCAG 4xmt-iATPSnFR1.0 was created by PCR of the DNA encoding iATPSnFR1.0 from Addgene Plasmid #102556 (a gift from Baljit Khakh) ...
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Cre/LoxP-HBV plasmids generating recombinant covalently closed circular DNA genome upon transfectionbioRxiv - Microbiology 2020Quote: ... a transfection assay was performed with a pCAG-LoxP-STOP-LoxP-ZsGreen [24] (gift from Pawel Pelczar; Addgene plasmid #51269) that would serve as a reporter for Cre recombination ...
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bioRxiv - Genomics 2019Quote: Melanoma cells and melanocyte growth assays were conducted using lentiviral transduction of MX2 cDNA under the control of tetracycline-inducible promoter using pINDUCER20 vector (Addgene). The MX2 cDNA clone (RC206437 ...
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bioRxiv - Molecular Biology 2023Quote: ... The nSauCas9D10A plasmid used for the orthogonal R-loop assay was also cloned by SDM using CMV-dSauCas9 (Addgene #138162) as a template ...
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bioRxiv - Cell Biology 2020Quote: ... inhibitor of growth protein-2 (ING2) cDNA was a gift from Curtis Harris (Addgene plasmid # 13294) (Pedeux et al ...
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bioRxiv - Molecular Biology 2020Quote: HeLa cells were infected with lentivirus carrying the Cas9-BFP (blue fluorescent protein) vector (Addgene 52962). HCT116 and HEK293T were transfected with the same vector using Lipofectamine 2000 (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: The majority of the constructs used for expressing aggregating proteins were obtained from Addgene (Watertown, MA). The Addgene catalog numbers are as follows ...
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bioRxiv - Biophysics 2019Quote: The cDNAs for protein expression in this study were as follows: human Tau-2N4R (Addgene #16316), human MAP7 (GE Dharmacon MGC Collection #BC025777) ...
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bioRxiv - Biophysics 2019Quote: ... both proteins were expressed in HEK293T cells using a modified pTT5 expression vector (Addgene no. 44006), purified using StrepTactin beads (GE ...
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bioRxiv - Cancer Biology 2020Quote: ... A plasmid expressing Green Fluorescent Protein (GFP) (pLJM1-EGFP was a gift from David Sabatini (Addgene plasmid # 19319 ...
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bioRxiv - Cancer Biology 2020Quote: HeLa cells were infected with lentivirus carrying the Cas9-BFP (blue fluorescent protein) vector (Addgene 52962). HCT116 were transfected with the same vector using Lipofectamine 2000 (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... The cDNA encoding EGFP-LC3 fusion protein was released from pBABE-EGFP-LC3-puro (Addgene, #22405) and cloned into pQCXIN-EGFP-N1-Neo by the 5’-EcoRI and 3’-AgeI sites ...
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bioRxiv - Cell Biology 2021Quote: ... Corresponding oligonucleotides were annealed and cloned in the pX330 plasmid expressing human SpCas9 protein (Addgene #42230). The donor plasmids were constructed as follows ...
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bioRxiv - Systems Biology 2023Quote: Jurkat cells expressing dCas9-KRAB fusion protein were constructed by lentiviral delivery of pMH0006 (Addgene #135448) and FACS isolation of BFP-positive cells.
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bioRxiv - Genomics 2022Quote: ... Protein A-MNase was expressed and purified from BL21(DE) carrying pET-pA-MN (Addgene: 86973).68–70
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 whole spike protein HexaPro was a gift from Jason McLellan (Addgene plasmid # 154754). SARS-CoV-2 HexaPro was expressed in Expi293F cells and purified with Ni-NTA resin followed by size exclusion as described previously [61] ...
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bioRxiv - Cell Biology 2023Quote: ... conjugated with the Rhotekin Rho Binding domain (RBD)-GST fusion protein (produced from Addgene plasmid #15247), for 1 hour at 4 °C ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... All clones for lentiviral vectors expressing the proteins described in this study are available from Addgene.
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bioRxiv - Biochemistry 2023Quote: Two vectors with C-terminal MBP (maltose-binding protein)-His6 Tag (2Cc-T; Addgene Plasmid #55209) or N-terminal His10-MBP Tag (2CT-10 ...
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bioRxiv - Bioengineering 2023Quote: ... the protein coding sequence (CDS) of RLuc8.6 was amplified from pcDNA-RLuc8.6-53559 (Addgene ID 87125) and subcloned into the vector pRSETb115 (Addgene ID 89536 ...
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bioRxiv - Cancer Biology 2024Quote: ... cell lines were transiently transfected with a membrane-targeted fluorescent protein (glycosylphosphatidylinositol-anchored eGFP, Addgene #32601) using a TransIT-X2 transfection kit (Mirus) ...
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bioRxiv - Cancer Biology 2024Quote: ... containing a C-terminus EGFP-tagged sequence of the full-length M237I p53 protein (Addgene, #11770), and 4 µL of Lipofectamine 2000 reagent ...
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bioRxiv - Genetics 2021Quote: ... These DNA fragments were subsequently assembled into a XbaI/AscI-linearized w+attB plasmid (Addgene, Watertown ...
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bioRxiv - Genetics 2021Quote: ... is intended for small-medium sized donor DNAs (<15kb) while the p5155 (Addgene reference 175391) is a low-copy version for use with larger donor DNAs (>15kb) ...
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bioRxiv - Cancer Biology 2021Quote: ... as oligonucleotide DNA sequences and cloned into pSpCas9(BB)-2A-GFP (PX458) (Addgene; Cat#: 48138) to construct two active CRISPR vectors ...
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bioRxiv - Plant Biology 2020Quote: ... Basic DNA parts were selected from GoldenBraid 2.0 kit from Diego Orzaez (Addgene kit # 1000000076) or MoClo Toolkit ...
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bioRxiv - Developmental Biology 2022Quote: E.coli strain BL21-DE3 was transformed with plasmid DNA pAG-MNase-6xHis (Addgene, plasmid #123461). Recombinant pAG-MNase was purified from cells grown in LB medium to OD600 0.6 at 37°C ...
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bioRxiv - Microbiology 2022Quote: pEMG-Tel (pEMGT) was constructed by cloning a DNA fragment from pMo130-TelR (Addgene, #50799) (bearing the Tel resistance marker ...
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bioRxiv - Cancer Biology 2022Quote: ... Oligonucleotide pairs were manufactured by Integrated DNA Technologies (IDT) and cloned into lentiCRISPRv2 (Addgene: #52961) as previously described (Sanjana et al. ...
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bioRxiv - Plant Biology 2021Quote: ... The final T-DNA vectors were generated using pDe-Cas9 as template (Addgene ID 61433) (Fauser et al. ...
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bioRxiv - Developmental Biology 2019Quote: ... the template DNA (pT3TS-nls-zCas9-nls; kind gift of Wenbiao Chen (Addgene plasmid # 46757) was linearized by XbaI ...