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Citations for Addgene :
301 - 350 of 10000+ citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... plentiCRIPSR v2 (a gift from Feng Zhang) was obtained from Addgene (Cat # 52961). pVSV-G and pCMVDR8.91 were a gift from Didier Trono ...
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bioRxiv - Synthetic Biology 2024Quote: ... human Cdh3 (PCAD) was amplified from plasmid pcDNA3 P-cad [bought from Addgene, catalog number #47502] ...
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bioRxiv - Cell Biology 2024Quote: ... Human NLRP3 from pEGFP-C2-NLRP3 (gift from Christian Stehlik (Addgene plasmid # 73955)) was inserted into an mCherry-C2 mammalian vector by Gibson assembly cloning (NEB ...
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bioRxiv - Microbiology 2020Quote: ... and the matrix protein from vesicular stomatitis virus was amplified from pVSV eGFP dG (a gift from Connie Cepko; Addgene plasmid #31842) as described above using the primers listed in Table S2 ...
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bioRxiv - Cell Biology 2021Quote: ... a gift from Dr. Rick Horwitz) and EGFP-RLC (no. 35680; a gift from Dr. Tom Egelhoff) were obtained from Addgene (Watertown, MA). 1-2 μg of plasmid was used to transfect 500,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5-10 μL ...
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bioRxiv - Neuroscience 2023Quote: ... The pX458-CAG-dCas9KRAB-2A-GFP plasmid was generated by subcloning the CAG promoter from pCAGGS and the dCas9KRAB sequence from CRISPR-dCas9KRAB (a gift from Kuniya Abe) into the pX458 vector (Addgene plasmid #48138). For CRISPR interference experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... pRRL-hPGK-mCherry-P2A-PuroR-T2A-FLAG-APEX2 was created by PCR amplifying hPGK-mCherry-P2A-PuroR-T2A from a synthesised gene fragment purchased from GeneWiz and APEX2 from pcDNA3-FLAG-APEX2-NES (a gift from Alice Ting67, Addgene plasmid # 49386) followed by insertion into PCR linearised pRRL backbone ...
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bioRxiv - Cancer Biology 2023Quote: Inducible Myc constructs were created from synthetic fragments (Twist Biosciences) sub-cloned into a general backbone derived from LT3GEPIR (gift from J. Zuber; Addgene plasmid #111177) using Gibson Assembly reagents (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... was amplified from pSMART-Rps3-HaloTag7-3xFlag (gift from Heeseon An) and P2A-puro was amplified from lentiCRISPRv2 puro (Addgene, cat # 98290). The amplified PCR products were cloned using GeneArt™ Seamless Cloning and Assembly Enzyme Mix (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... FOXM1 coding sequence including a Flag-tag was used from a Flag-FOXM1 plasmid (#153136 from Addgene; kind gift from Stefan Koch) (Moparthi and Koch ...
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bioRxiv - Cell Biology 2024Quote: ... along with DsRed (excised from the tdTomato sequence, derived from pCDH-EF1-Luc2-P2A-tdTomato, Addgene number 72486, a gift from Kazuhiro Oka), under the tight TRE promoter ...
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bioRxiv - Molecular Biology 2021Quote: ... The mCherry expression cassette from pCAGGS-mCherry (a gift from Phil Sharp, Addgene #41583) was inserted between the homology arms ...
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bioRxiv - Cancer Biology 2021Quote: ... ZsGreen was subcloned from pHIV-zsGreen (gift from Bryan Welm & Zena Werb (Addgene #18121))79.
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bioRxiv - Genomics 2021Quote: ... and HP1α(Δ1-72) were cloned from GFP-HP1α (gift from Tom Misteli, Addgene plasmid #17652
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bioRxiv - Neuroscience 2020Quote: ... rTetR and tight TRE promoter from pCW57.1 (a gift from David Root, Addgene #41393); hPKG promoter (M60581.1) ...
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bioRxiv - Cell Biology 2021Quote: ... into an N1 cloning vector (cut from Addgene #54642, a gift from Michael Davidson), and then replacing mCherry with mOrange2 (cloned from Addgene #57969 ...
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bioRxiv - Cell Biology 2021Quote: ... into an N1 cloning vector (cut from Addgene #54642, a gift from Michael Davidson). mOrange2-Parkin was constructed by first inserting mCherry-Parkin (cloned from Addgene #59419 ...
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bioRxiv - Molecular Biology 2021Quote: ... iRFP720 was obtained by PCR from iRFP720-N1 (gift from Vladislav Verkhusha, Addgene #45461).
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bioRxiv - Neuroscience 2021Quote: ... TVA-mCherry was amplified by PCR from a plasmid (gift from Dr. Uchida, Addgene plasmid #38044 ...
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bioRxiv - Biochemistry 2021Quote: The Cas12a gene from Francisella novicida was amplified from the plasmid pFnCpf1_min (Addgene #69975) (1 ...
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bioRxiv - Bioengineering 2021Quote: ... The Spike protein from pcDNA3.1-SARS2-Spike was a gift from Fang Li (Addgene plasmid # 145032 ...
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bioRxiv - Cell Biology 2022Quote: ... OMP25 was PCR amplified from paGFP-Omp25 (gift from D. Sabatini, Addgene plasmid #69598) and cloned with XhoI/BamHI into mMaple-C1 ...
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bioRxiv - Cell Biology 2022Quote: ... RA was PCR amplified from RA-NES (gift from R. Campbell, Addgene plasmid #61019) (Ding et al. ...
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bioRxiv - Cell Biology 2022Quote: ... GB was PCR amplified from GB-NES (gift from R. Campbell, Addgene plasmid #61017) (Ding et al. ...
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bioRxiv - Immunology 2022Quote: Class-switched VRC07 constructs were generated from human isotype backbone plasmids obtained from Addgene. The different human heavy chain constant regions were PCR amplified from the Addgene plasmids and inserted into the previously described AAV transfer vector 11 encoding the VRC07 heavy chain variable region and VRC07 kappa light chain ...
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bioRxiv - Genomics 2022Quote: ... The SpCas9-NG ORF was subcloned from pX330-SpCas9-NG obtained from Addgene (#117919), and assembled with TEF1 promoter and CYC1 terminator (Zhang et al. ...
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bioRxiv - Microbiology 2022Quote: LentiCas9-Blast plasmid was a gift from Feng Zhang and was obtained from Addgene (Addgene plasmid # 52962 ...
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bioRxiv - Molecular Biology 2022Quote: ... we amplified cpGFP and FHA1 from pcDNA3.1(+)-ExRai-AKAR2 (gift from Jin Zhang, Addgene plasmid # 161753 ...
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bioRxiv - Neuroscience 2024Quote: ... and using EcoRI into pAAV_hSyn_mEGFP (generated from moxBFP, a gift from Erik Snapp, Addgene plasmid #68064 ...
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bioRxiv - Molecular Biology 2023Quote: ... the Kozak-3XFLAG fragment (from c3GIC9, Addgene #62191, a gift from Lukas Dow 109), the PRKRA CDS (amplified from a Mael-/- testis cDNA sample ...
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bioRxiv - Cell Biology 2023Quote: ... The following plasmids were both gifts from Michael Nick Boddy and bought from Addgene: pFA6a-KanMX-tetO-pCyc1 (TetP ...
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bioRxiv - Immunology 2023Quote: ... psPAX2 and pMD2.G (gifts from from Didier Trono, Addgene plasmids # 12260 and # 12259). Lentiviral particles were harvested from the supernatant and concentrated 100X using PEG precipitation ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid HaloTag-KEAP1 (a gift from Yimon Aye, plasmid # 58240) was purchased from Addgene. HiBit-CDK9 were constructed through the Gibson assembly method ...
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bioRxiv - Genomics 2023Quote: ... and Cas9-gRNA plasmid pX459-EN1201 (backbone from Addgene plamid #62988, guide from Addgene plasmid #9214434 ...
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bioRxiv - Molecular Biology 2023Quote: ... Full-length ELK1 gene was amplified from pCGN-ELK1 purchased from Addgene (plasmid #27156) using a set of primers (Table S1 ...
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bioRxiv - Neuroscience 2023Quote: ... with the CaMKII promoter from mCh-GluA1-CIB (a gift from Matthew Kennedy (Addgene plasmid # 89444 ...
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bioRxiv - Neuroscience 2024Quote: ... we isolated mScarlet from pmScarlet_C1 (a gift from Dr. Dorus Gadella; Addgene plasmid # 85042) through pcr amplification ...
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bioRxiv - Neuroscience 2024Quote: ... HA-SUMO2 was subcloned from pcDNA-HA-SUMO2 (gift from Guy Salvesen, Addgene #48967) and inserted into the pL302 lentiviral backbone (gift from Jacqueline Burré and Thomas Südhof at Stanford) ...
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bioRxiv - Developmental Biology 2024Quote: ... DsRed was amplified from pScarlessHD-sfGFP-DsRed (Addgene #80811, gift from Kate O’Connor-Giles), and homology arms were amplified from genomic DNA of vas-Cas9 III (BDSC #51324) ...
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bioRxiv - Cell Biology 2024Quote: ... Cre recombinase was expressed from a plasmid derived from pPGK-Cre-bpA (Addgene #11543) with an SV40polyA sequence instead of the bpA ...
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bioRxiv - Synthetic Biology 2024Quote: ... human CAV1 (Caveolin-1) was amplified from plasmid mCherry-Caveolin-C10 [bought from Addgene, catalog number #55008] ...
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bioRxiv - Neuroscience 2024Quote: ... were commercially obtained from the Boston Children’s Hospital Viral Core or directly from Addgene. Virus aliquots were stored at −80 °C ...
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bioRxiv - Biochemistry 2024Quote: ... mCherry was subcloned from H2B-mCherry (a gift from Robert Benezra; Addgene plasmid # 20972)88 into pENTR1a by flanking XhoI/XbaI sites prior to transferring to pLenti CMV Blast ...
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bioRxiv - Biochemistry 2024Quote: ... following PCR amplification from the pmCherry-NLS plasmid (a gift from Martin Offterdinger; Addgene plasmid # 39319 ...
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bioRxiv - Cell Biology 2024Quote: ... from the pSpCas9(BB)-2A-Puro (PX459) V2.0 plasmid gifted from Feng Zhang (Addgene plasmid # 62988 ...
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bioRxiv - Genetics 2024Quote: ... used in this study were separately obtained from previously reported plasmids available from Addgene. Other ABE variant expression plasmids were constructed by replacing the deaminase and Cas-nuclease domains using a Gibson assembly of linearized destination vector and PCR amplicons with Gibson-compatible ends for each assembly junction ...
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bioRxiv - Synthetic Biology 2021Quote: ... hCas9 (obtained from Addgene, #41815) was used as the template ...
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bioRxiv - Cancer Biology 2021Quote: ... was obtained from Addgene (30,31). The pGIPZ was purchased from Open Biosystems (vector # RHS4346) ...
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bioRxiv - Cell Biology 2020Quote: ... was obtained from Addgene (#40276) (Mulleder et al. ...
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bioRxiv - Cell Biology 2020Quote: ... LifeAct-GFP was from Addgene.