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Citations for Addgene :
201 - 250 of 2714 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The following day, cells were co-transfected (using Lipofectamine 3000 treatment, as described above) with pMD2.g (envelope plasmid, Addgene #12259), psPAX2 (packaging plasmid ...
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bioRxiv - Genomics 2023Quote: ... Lentivirus containing the landing pad sequence was produced by co-transfecting 293T cells at ∼50% confluence with psVSV-G (Addgene #12259), psPAX2 (Addgene #12260) ...
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bioRxiv - Microbiology 2022Quote: Lentiviral particles were generated by co-transfection of the expression constructs and 2nd generation packaging plasmids (psPAX2, Addgene#12260 & pMD2.G, Addgene#12259), using jetPEI DNA transfection reagent (Polyplus transfection ...
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bioRxiv - Neuroscience 2023Quote: ... pLVX-UbC-rtTA-Htt-Q94-CFP vector was co-transfected in HEK293T cells using Lipofectamine2000 with packaging vectors pMD2.G (Addgene, #12259) and psPAX2 (Addgene ...
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bioRxiv - Molecular Biology 2024Quote: HEK293 cells in 15cm dishes at ∼80% confluency were co-transfected with 12μg (each) lentiviral vector together with 9μg psPAX2 and 3μg pMD2.G (Addgene, 12260 and 12259, respectively). 6 h later ...
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bioRxiv - Biochemistry 2024Quote: ... HEK-293T cells were co-transfected with UVSSA- or UVSSA-K414R-encoding plasmid together with packaging plasmid pMD2.G (Addgene, #12259) and psPAX2 (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... Lentiviruses were produced by co-transfecting HEK293T cells with the packaging plasmids psPAX2 and pMD2.G (Addgene plasmid # 12260 and 12259) and the transfer plasmid LentiCRISPRv2-gRNAs ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids co-expressing SEPT2-sfCherry2 and SEPT6 were co-transformed with plasmids co-expressing SEPT7 and SEPT9_i3 (Addgene #174501) to generate recombinant SEPT2-sfCherry2 octamers-9_i3 ...
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bioRxiv - Bioengineering 2023Quote: ... followed by addition of the plasmid cocktail containing 4 µg of pMD2.G (a gift from Didier Trono; Addgene plasmid # 12259; http://n2t.net/addgene:12259; RRID:Addgene_12259), 10 µg of psPAX2 (a gift from Didier Trono ...
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bioRxiv - Cell Biology 2023Quote: Lentiviral particles were prepared in HEK293T cells using standard calcium-phosphate transfection of the lentivector with packaging plasmids pMD2.G (Trono lab, Addgene #12259) and pCMV-dR8.74 (Trono lab ...
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bioRxiv - Cell Biology 2022Quote: ... were co-transformed with plasmids co-expressing SEPT6 and SEPT7 (Addgene #174499), or SEPT7 and SEPT9_i3 (Addgene #174501) ...
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bioRxiv - Molecular Biology 2021Quote: VSV-G envelope pseudo-typed lentivirus for the generation of stable cell lines was produced by co-transfection of each transfer plasmid with pCMVdR 8.91 71 and pCMV-VSV-G (gift from Prof. Weinberg, Addgene plasmid # 8454 72). 72 h post-transfection ...
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bioRxiv - Immunology 2021Quote: Lentiviruses were generated by co-transfection of lentiCRISPR v2 constructs and packaging plasmids (psPAX2, Addgene no. 12260 and pMD2.G, Addgene no. 12259), using PEI DNA transfection reagent (Shanghai maokang biotechnology) ...
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bioRxiv - Immunology 2023Quote: ... were co-transfected with the pHR plasmids and second-generation lentiviral packaging plasmids pMD2.G and psPAX2 (Addgene plasmid #12259 and #12260) using Genejuice transfection reagent (EMD Millipore ...
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bioRxiv - Microbiology 2023Quote: Lentiviruses were generated by co-transfection of vector constructs and second-generation packaging plasmids (psPAX2, Addgene no. 12260 and pMD2.G, Addgene no. 12259), using jetPEI DNA transfection reagent (Polyplus transfection) ...
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bioRxiv - Cancer Biology 2022Quote: ... Lentivirus was generated using 293FT cells by co-transfection of guide plasmid with psPAX2 and pMD2.G (Addgene, 12260 and 12259, respectively). Conditioned media was harvested ...
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bioRxiv - Immunology 2024Quote: Lentiviral particles containing the miBds libraries were produced by lipofectamine-based co-transfection of HEK293 cells with 3rd generation packaging plasmids pMD2.G (Addgene, Cat.#12259), pMDLg/pRRE (Addgene ...
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bioRxiv - Molecular Biology 2024Quote: Lentiviral expression vectors were co-transfected into 293FT cells with the lentiviral packaging plasmids psPAX2 and pMD2.G (Addgene #12260 and #12259). Briefly ...
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bioRxiv - Cell Biology 2024Quote: Second generation lentiviral particles were prepared by co-transfecting HEK-293T cells with the plasmid of interest (pMD2.G (Addgene, cat. #12259), psPAX2 (Addgene ...
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bioRxiv - Bioengineering 2023Quote: ... followed by addition of the plasmid cocktail containing 4 µg of pMD2.G (a gift from Didier Trono; Addgene plasmid # 12259 ...
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bioRxiv - Microbiology 2024Quote: ... before co-transfection the next day with 15 µg of the pLVX-3xHA-TurboID-RAB11A plasmid along with 10 and 5 µg of the pΔ8.74 (Addgene #22036) and pMD2.G (Addgene #12259 ...
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bioRxiv - Cell Biology 2022Quote: ... The control virus with VSV-G as the tropism and expression of mCherry was generated by co-transfection of pLV-mCherry and pMD2.G vector (Addgene, Watertown, MA, USA) into the Phoenix cells ...
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bioRxiv - Cell Biology 2020Quote: ... the Mouse GeCKOv2 CRISPR knockout pooled library plasmids were co-transfected with packaging plasmids pCMV-VSV-G and pCMV-dR8.2 dvpr (Gifts from Bob Weinberg, Addgene plasmid #8485 and #8455) into HEK293T cells ...
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bioRxiv - Microbiology 2022Quote: Lentiviral particles were generated by co-transfection of the expression constructs and 2nd generation packaging plasmids (psPAX2, Addgene#12260 & pMD2.G, Addgene#12259), using jetPEI DNA transfection reagent (Polyplus transfection ...
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bioRxiv - Cell Biology 2020Quote: ... and VSV-G (VSV-G: pMD2.G, Addgene, 12259) was performed using lipofectamine 2000® (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... and VSV-G (VSV- G: pMD2.G, Addgene, 12259) was performed using Lipofectamine 2000® (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral particles were produced in HEK293T cells by transfection with 0.2 µg of pCMV-VSV-G (Addgene, #8454, MA, USA), 2 µg of psPAX2 (Addgene ...
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bioRxiv - Neuroscience 2024Quote: ... 200 µg of pH GT1-ademo/dF6 helper and 70 µg of pGP-AAV-syn-jGCaMP7b-WPRE (Plasmid Number 104489, Addgene)56 and mix with 18 ml OptiMEM (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... 2 µg of pX330 plasmid containing sgRNA for the target protein and 0.2 µg of pcDNA3-FKBP-eGFP-HOTag3 (Addgene, #106924) were co-transfected using Lipofectamine 2000 (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The following viruses (Addgene Co) were used ...
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bioRxiv - Genetics 2020Quote: Barcoded MSH2-2A-blR cDNA libraries were packaged into lentivirus by co-transfecting HEK293T/17 cells (ATCC) with the transfer plasmid pool plus envelope and packaging vectors (pMD2.G, Addgene #12259 and psPAX2, #12260). For each pool ...
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bioRxiv - Developmental Biology 2023Quote: ... lentiviral supernatants were generated by transfecting one 10-cm plate of HEK-293T cells at 90% confluency with 3 µg pMD2.G (Addgene #12259), 9 µg psPAX2 (Addgene #12260) ...
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bioRxiv - Cancer Biology 2020Quote: ... MSCV vector containing either nothing (empty vector) or the CTAG1A gene were co-transfected in 293FT with pMD2.G and pUMVC (Addgene #8449, gift from Bob Weinberg) at a ratio of ∼1.5:1.2:1.8 ...
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bioRxiv - Microbiology 2022Quote: Lentivirus was produced in HEK293T by co-transfection of cDNA containing lentiviral plasmid together with helper plasmids pMD2.G (Addgene #12259, gift from Didier Trono) and pCMV-dR8.91 (Life Science Market ...
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bioRxiv - Cancer Biology 2022Quote: ... at 70-80% confluency were co-transfected with the lentiviral plasmid and lentiviral helper plasmids (psPAX2: Addgene #12260 and pMD2.G: Addgene #12259, gifts from Didier Trono) using PureFection (SBI System Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: Retrovirus vectors were generated by co-transfecting the LKB1-containing plasmids with the pCMV-VSV-G packaging plasmid (Addgene 8454, a gift from Robert Weinberg) into GP2-293 cells (Takara 631458) ...
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bioRxiv - Cancer Biology 2023Quote: ... Transfer lentiviral plasmids (2500 ng) were diluted in 200 µl serum-free medium together with 225 ng pCMV-VSV-G (Addgene #8454) and 2250 ng psPAX2 (Addgene #12260 ...
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bioRxiv - Cell Biology 2021Quote: ... The co-expression plasmid of free Clover and mRuby2 was constructed by inserting an internal ribosome entry site (cloned from Addgene #127332; a gift from Jeremy Wilusz) between the Clover and mRuby2 of pcDNA3.1-Clover-mRuby2 at the AgeI site ...
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bioRxiv - Neuroscience 2020Quote: ... we co-electroporated pmRFP-LC3 (Addgene, #21075 ...
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bioRxiv - Cell Biology 2022Quote: ... and co-injection markers (pGH8 Addgene #19359 ...
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bioRxiv - Cell Biology 2024Quote: ... co-transfections with mRFPRuby-LifeAct (Addgene plasmid #51009 originally from Rusty Lansford ...
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bioRxiv - Cancer Biology 2020Quote: ... 5000 ng of psPAX2 (Addgene plasmid #12260), 500 ng of pMD2.G (Addgene plasmid #12259) ...
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bioRxiv - Cancer Biology 2021Quote: ... 5000 ng of psPAX2 (Addgene plasmid #12260), 500 ng of pMD2.G (Addgene plasmid #12259) ...
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bioRxiv - Microbiology 2022Quote: All lentiviruses vector particles were generated in HEK-293T cells by co-transfection with plasmids pMD2.g and psPAX2 (Addgene # 12259, #12260, gift of Didier Trono), exactly as described previously (43) ...
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bioRxiv - Molecular Biology 2021Quote: ... with VSV-G envelope (pMD2.G; Addgene) and packaging (psPAX2 ...
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bioRxiv - Microbiology 2022Quote: ... and pMD2.G (encoding VSV-G) (Addgene) were transfected in equimolar amounts using Lipofectamine 3000 (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... VSV-G (pMD2.G, Addgene Plasmid #12259) in 1:1:1 molar ratio) ...
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bioRxiv - Microbiology 2023Quote: ... VSV-G plasmid (pMD2.G Addgene 12259), and pCMV delta R8.2 (Addgene 12263) ...
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bioRxiv - Neuroscience 2024Quote: ... and VSV-G (pMD2.G, Addgene, #12259). Cells were seeded in a 15 cm dish in 22.5 ml medium (DMEM with 4.5g/l glucose and pyruvate ...
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bioRxiv - Microbiology 2023Quote: ... a vesicular stomatitis virus G (VSV-G) envelope expression vector (pMD2.G, #12259, Addgene) and a lentiviral transfer vector containing the transgene in a ratio of 2:1:3 by calcium phosphate transfection ...