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Citations for Addgene :
151 - 200 of 2714 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: HEK293T cells were grown in 10-cm dishes to 80% confluency before transfection with the lentiviral vector (10 µg) with packaging vectors including pMD2.G (3 µg, Addgene plasmid # 12259), psPAX2 (6 µg ...
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bioRxiv - Developmental Biology 2021Quote: We grew HEK293T cells in 10-cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 µg) with packaging vectors including pMD2.G (3 µg, Addgene plasmid # 12259), psPAX2 (6 µg ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293T cells were seeded at 80% density in a 10-cm tissue cell culture treated dish and transfected with the 6 µg of expression plasmid and packaging plasmids 2 µg of pMD2.G (Addgene, cat# 12259) and 4 µg of psPAX2 (Addgene ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were co-transfected with lentiviral constructs for overexpression with the packaging vectors Δ8.9 and VSV-G (Addgene) using FuGENE®-6 (Promega) ...
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bioRxiv - Genomics 2021Quote: ... the plasmids were co-transfected with the packaging plasmids psPAX2 and pMD2.G (Addgene #12260 and #12259, respectively) into 293FT cells (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... then 5μg of desired lentiviral vector was co-transfected with 2.5μg of envelope protein vector pMD2.G (Addgene:12258), and 2.5μg of the packaging vector psPAX2 (Addgene ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells were used to package lentiviruses by co-transfecting viral packaging plasmids pCMV-VSV-G (Addgene #8454), psPAX2 (Addgene #12260 ...
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bioRxiv - Bioengineering 2023Quote: ... Lentiviruses were packaged using 293FT cells by co-transfection of lentiviral transfer plasmids with pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260 ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviral particles were generated by co-transfecting the respective vectors with the packaging plasmids pMD2.G (RRID: Addgene_12259) and psPAX2 (RRID ...
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bioRxiv - Molecular Biology 2024Quote: ... Parental HEK293 cells were co-transfected with 1.0 µg each of PBKS-Cas9-2A-eGFP plasmid (Addgene plasmid #68371) and plasmid encoding for gRNA ...
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bioRxiv - Molecular Biology 2022Quote: ... a 2 mL transfection mixture containing lentiviral packaging plasmids 7.5 µg pMD2.G (#12259, Addgene), 11.4 µg pMDLg-RRE (#12251 ...
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bioRxiv - Bioengineering 2024Quote: ... and the following morning the cells were transfected with 12.1 µg pMD2.G (Addgene #12259), 18.15 µg psPAX2 (Addgene #12260 ...
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bioRxiv - Cell Biology 2024Quote: ... then transfecting with 1 µg equimolar packaging mix (pMDL, Addgene 1251; pRSV, Addgene1253; pVSV-g) and rtTA (phr_tet3g ...
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bioRxiv - Cell Biology 2021Quote: ... The plasmids were then co-transfected separately with third-generation lentiviral packaging and envelope vectors: pMD2.G (Addgene #12259), pRSV-Rev (Addgene #12253) ...
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bioRxiv - Microbiology 2020Quote: ... were co-transfected with pLenti plasmids and the packaging plasmids psPAX2 and pMD2G/VSV-G (Addgene Plasmids #12259-60) to produce lentiviral particles ...
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bioRxiv - Cell Biology 2022Quote: Lentivirus was generated by co-transfecting HEK293T cells with two packaging plasmids (pCMV-VSV-G and delta8.9, Addgene #8454) and the desired transfer plasmid using TransIT-293 transfection reagent (Mirus) ...
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bioRxiv - Cancer Biology 2024Quote: ... Lentiviral particles were generated by co-transfecting LentiGuide-sgRNA constructs and packaging constructs (psPAX2, RRID:Addgene_12260; and pMD2.G, RRID:Addgene_12259) to 293T cells ...
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bioRxiv - Cell Biology 2023Quote: ... The lentiviral vectors were co-transfected into 293T cells with the packaging vectors pCMV-dR8.2 dvpr and pCMV-VSV-G (Addgene). Virus-infected cells were selected with 2 μg/ml puromycin ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293-FT cells at 70% confluence were co-transfected with the plasmid of interest (0.50 μg DNA/ reaction) and lentiviral packaging vectors (pMD2.G Addgene plasmid #12259 ...
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bioRxiv - Immunology 2023Quote: ... a 4-1BB costimulatory domain and the CAR construct utilized for animals R.301-304 additionally expressed EGFRt.23 CARs were encoded by an xHIV plasmid which was co-transfected with an HIV-1 Rev/Tat and VSV-G envelope plasmid (RRID:Addgene_138479) for lentiviral production as previously described.23
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bioRxiv - Cell Biology 2024Quote: ... and 24 hours later the target plasmid was co-transfected together with the pCMV-VSV-G (Addgene plasmid # 8454) (80 ...
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bioRxiv - Cell Biology 2023Quote: HEK293T-LentiX cells were co-transfected for 12 h with 10 µg psPAX2 (kind gift from Didier Trono, Addgene #12260), 2.5 µg pMD2.G (kind gift from Didier Trono ...
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bioRxiv - Cell Biology 2024Quote: ... cells were seeded per well of a 6-well plate and co-transfected with 2.8 µg pHAGE-mKeima-LGALS3 (Addgene; 175780), 2.3 µg pPAX2 (Addgene ...
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bioRxiv - Cancer Biology 2020Quote: ... to co-transfect pBABE-puro or pBABE-puro.SLX4IP.3xFLAG with pCMV-VSV-G (at a ratio of 6:1, Addgene#8454) into GP2-293 cells (Clontech) ...
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bioRxiv - Cancer Biology 2020Quote: ... and lentiviral particles for sgRNA were produced in HEK293T cells by co-transfecting pLXsgRNA plasmid with pMD2.G (Addgene# 12259) and psPAX2 (Addgene #12260).(Supplementary table 2a ...
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bioRxiv - Cancer Biology 2022Quote: ... and each plasmid was packaged separately in 293T cells via co-transfection with polyethylenimine alongside pCMV-VSV-G (Addgene #8454) envelope plasmid and pCMV-dR8.2 dvpr (Addgene #8455 ...
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bioRxiv - Microbiology 2021Quote: ... The library lentivirus was generated by co-transfection of the library plasmid mixture with two lentiviral packaging plasmids pR8.74 and pVSV-G (Addgene, 12259) as a proportion of 10:10:1 into HEK293T cells ...
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bioRxiv - Immunology 2020Quote: ... Generation of shRNA IFIT1 cells: 293T cells were co-transfected with psPax2 and pMD2.G (Addgene plasmids #12260 and #12259), as well as pLKO.1 (50 ...
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bioRxiv - Immunology 2022Quote: ... Each pHIV-EGFP construct was co-transfected with package plasmids pCMV-VSV-G (addgene, 8454) and pCMV-dR8.2 dvpr (Addgene, 8455) into HEK 293T cells for 96 h ...
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bioRxiv - Cancer Biology 2020Quote: HEK293T cells were co-transfected with pLX304 plasmids containing constructs of interest and the packaging plasmids pMD2.G (Addgene, #12259) and psPAX (Addgene ...
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bioRxiv - Genetics 2021Quote: ... Lentivirus was produced by polyethylenimine (PEI) co-transfection of the desired construct and two packaging vectors VSV-G (Addgene #8454) and psPAX2 (Addgene #12260 ...
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bioRxiv - Cancer Biology 2021Quote: ... Lentivirus was produced by co-transfection of the TRIPZ shRNA with pMD2.G and psPAX2 (gifts from Didier Trono, Addgene plasmid #12259 and #12260 ...
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bioRxiv - Molecular Biology 2021Quote: The pLKO.1-plasmids for REST/NRSF knockdown were co-transfected into HEK293T cells with the psPAX2 and pMD2.G helper plasmids (Addgene) using Lipofectamine 2000 (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: The lentiviral particles expressing individual cDNA labelled with an mCherry marker were generated by co-transfection of the cDNA plasmid mixture with two lentiviral packaging plasmids pR8.74 and pVSV-G (Addgene, 12259) as a proportion of 10:10:1 into HEK293T cells ...
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral constructs were packaged in 293FT cells using co-transfection of pLVX constructs with with psPAX2 and pMD2.G (Addgene). Virus titers were estimated using puromycin resistance in HEK-293T cells ...
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bioRxiv - Genomics 2024Quote: ... Lentivirus was produced in HEK293T cells co-expressing the shRNA plasmid together with psPAX2 packaging plasmid and pVSV-G envelope plasmid (Addgene). Virus was concentrated using Lenti-X Concentrator (Takara ...
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bioRxiv - Cell Biology 2023Quote: ... the lentiviral vectors encoding each SLX4 cDNA (kindly provided by Dr. Minoru Takata) were individually co-transfected with two assistant vectors pMD2.G (#12259, Addgene) and psPAX2 (#12260 ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentivirus supernatants were prepared by co-transfecting the Tet-pLKO-puro plasmid along with helper plasmids pVSV-G (Addgene #138479) and p8.92 (Addgene #132929 ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviral supernatants were by transient co-transfection of individual constructs with packaging plasmids (psPAX2, Addgene #12260 and pMD2.G, Addgene #12259 into HEK-293T cells using X-tremeGENE HP DNA transfection reagent (#06366236001 ...
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bioRxiv - Immunology 2023Quote: ... LentiCRISPRv2–sgRNA Myc transfer plasmids was co-transfected with packaging plasmids psPAX2 and pMD2.G (Addgene plasmids #12259 and #12260) into HEK293T cells ...
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bioRxiv - Cell Biology 2023Quote: After production of recombinant lentiviruses in HEK293T cells by co-transfection of the pLentiCRISPRv2 constructs with pMD2.G and psPAX2 (Addgene plasmids #12259 and #12260 ...
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bioRxiv - Neuroscience 2024Quote: ... Lentivirus encoding both LifeAct-RFP and EB3-YFP (cloned in a PLV-mCherry derived vector) were produced by co-transfection with the psPAX2 and pCMV-VSV-G helper plasmids (Addgene plasmids # 12260 and # 8454 ...
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bioRxiv - Molecular Biology 2024Quote: ... Lentivirus was produced by co-transfecting HEK293T cells with pCMV-VSV-G (a gift from Bob Weinberg; Addgene plasmid #8454), psPAX2 (a gift from Didier Trono ...
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bioRxiv - Cell Biology 2024Quote: ... control or mir-218-1-overexpressing HTR8 cells were seeded into 12-well plates and were co-transfected with 1 µg/mL of 5X NFκB luciferase reporter (64) (Addgene plasmid #12453) construct and 20 ng/mL of Renilla luciferase vector (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... The vector V2 CRISPR DNA Plasmid (1ug) was co-transfected in 293T cells along with 3 µg of the viral envelope PMD2 (Addgene # 12259) and 4 µg of the viral packing PsPAX (Addgene #12260 ...
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bioRxiv - Biophysics 2023Quote: ... HEK293T cells were seeded at a confluency of ∼70 % in T75 flasks and co-transfected 6 hours later with 7.5 µg of the lentiviral packaging vector psPAX2 (gift from Didier Trono - Addgene plasmid # 12260) and 15 µg of the plasmid encoding the respective viral surface protein (pCMV14-3X-Flag-SARS-CoV-2 S was a gift from Zhaohui Qian - Addgene plasmid # 145780 ...
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bioRxiv - Cancer Biology 2022Quote: ... 73 µg psPAX2 and 39.5 µg pMD2.G (gifts from Didier Trono (Addgene plasmid # 12260; http://n2t.net/addgene:12260; RRID:Addgene_12260)) using CaPO4 precipitation transfection 44 ...
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bioRxiv - Cell Biology 2024Quote: ... and 7 µg pMD2.G (gift from Didier Trono, Addgene plasmid #12259; http://n2t.net/addgene:12259; RRID:Addgene_12259) using JetPEI (Polyplus Transfection ...
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bioRxiv - Microbiology 2021Quote: ... the mutant constructs were co-transfected with psPAX2 and also the VSVg plasmid pMD2.G (Addgene; courtesy of Dr Didier Trono) at a molar ratio of 2:1:1 to increase the infection rate ...
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bioRxiv - Microbiology 2022Quote: Lentiviral particles were generated by co-transfection of the expression constructs and 2nd generation packaging plasmids (psPAX2, Addgene#12260 & pMD2.G, Addgene#12259), using jetPEI DNA transfection reagent (Polyplus transfection ...